In Vivo Evaluation of Strontium-Containing Nanostructured Carbonated Hydroxyapatite

2016 ◽  
Vol 696 ◽  
pp. 212-222
Author(s):  
Gabriel Maia Kammer ◽  
Suelen Cristina Sartoretto ◽  
Rodrigo Resende ◽  
Marcelo Uzeda ◽  
Jhonathan Raphael Nascimento ◽  
...  
Keyword(s):  
Sham Group ◽  
Subcutaneous Tissue ◽  
Biological Response ◽  
Experimental Period ◽  
Giant Cells ◽  
Control Group ◽  
X Ray Diffraction ◽  
Carbonated Hydroxyapatite ◽  
X Ray

Bone tissue is a composite material that has hydroxyapatite (HA) as its main inorganic phase component. The biological apatites have low crystallinity and contain cationic and anionic substitutions in their structure, which differ from the available synthetic ceramics. The purpose of this study is to evaluate the biocompatibility of nanostructured carbonated hydroxyapatite microspheres containing 5 wt% strontium (SrcHA) compared with the biocompatibility of carbonated hydroxyapatite (cHA), both synthesized at 37°C and non-sintered, used to control stoichiometric HA microspheres in subcutaneous tissue of mice. The biomaterials (BM) were characterized using X-ray Diffraction (XRD), Vibrational Spectroscopy in an Infrared Fourier Transform (VSIRFT) and Scanning Electron Microscopy (SEM). Forty five balb-C mice were randomly divided into four groups of 15 animals each: SrcHA, cHA, HA, and without material implantation (Sham group). All samples were histologically processed for descriptive evaluation of the biological effect. At each experimental period (1, 3 and 9 weeks), there was a higher biosorption of the tested biomaterials observed in contrast with the HA. The cHA group was the only group completely phagocytosed by macrophages and giant cells after 9 weeks. All biomaterials proved to be biocompatible, and the cHA and SrcHA 3% groups exhibited a faster bioabsorption in comparison with the control group. The doping of strontium did not cause a greater biological response after the 3 experimental periods.

In Vivo Evaluation of Zinc-Containing Nanostructured Carbonated Hydroxyapatite

2016 ◽  
Vol 696 ◽  
pp. 223-229 ◽  
Author(s):  
Simone Ribeiro ◽  
Suelen Cristina Sartoretto ◽  
Rodrigo Resende ◽  
Marcelo Uzeda ◽  
Adriana Terezinha Alves ◽  
...  
Keyword(s):  
Sham Group ◽  
Subcutaneous Tissue ◽  
Descriptive Analysis ◽  
Microscopic Analysis ◽  
Experimental Period ◽  
Negative Control ◽  
X Ray Diffraction ◽  
Carbonated Hydroxyapatite ◽  
Low Solubility

The hydroxyapatite (HA) is a biocompatible and bioactive biomaterial used as bone substitute, however, the high crystallinity of HA and consequently its low solubility may be a limitation for its clinical use. In order to improve the biosorption of HA, the partial substitutions in the chemical structure and doping with small amounts of impurities have been study. The objective of this study was to evaluate the biocompatibility of 3% Zinc-containing nanostructured carbonated hydroxyapatite (ZncHA) compared with the carbonated hydroxyapatite (cHA), both synthesized at 37°C and non-sintered, using as control the stoichiometric HA microspheres in subcutaneous of mice. The X-ray Diffraction (XRD) and Vibrational Spectroscopy in Infra Red Fourier Transform (FTIR) were used to characterize the biomaterials. In vivo test was performed in BALB/c mice by implanting of HA, cHA and ZncHA spheres in the subcutaneous tissue for 1, and 9 weeks (n=5). The negative control consisted in incision without material implantation (Sham group). The samples were histological processed to descriptive analysis of biological effect. The microscopic analysis showed a similar granulation reaction between groups at the first experimental period. In 9 weeks there was a time dependent biosorption of cHA compared with other groups. In conclusion, the biomaterials tested were biocompatible and cHA group showed a significant biosorption in comparison with HA and ZncHA groups. The doping of zinc did not influence the biocompatibility of biomaterial, however, change the biosorption response

Effects of dietary alteration of bicarbonate and magnesium on rat bone

1986 ◽  
Vol 250 (2) ◽  
pp. F302-F307 ◽  
Author(s):  
J. M. Burnell ◽  
C. Liu ◽  
A. G. Miller ◽  
E. Teubner
Keyword(s):  
Crystal Structure ◽  
Bone Formation ◽  
X Ray Diffraction ◽  
X Ray ◽  
Growing Rats ◽  
Final Composition ◽  
Rat Bone

To study the effects of bicarbonate and magnesium on bone, mild acidosis and/or hypermagnesemia were produced in growing rats by feeding ammonium chloride and/or magnesium sulfate. Bone composition, quantitative histomorphometry, and mineral x-ray diffraction (XRD) characteristics were measured after 6 wk of treatment. The results demonstrated that both acidosis (decreased HCO3) and hypermagnesemia inhibited periosteal bone formation, and, when combined, results were summative; and the previously observed in vitro role of HCO3- and Mg2+ as inhibitors of crystal growth were confirmed in vivo. XRD measurements demonstrated that decreased plasma HCO3 resulted in larger crystals and increased Mg resulted in smaller crystals. However, the combined XRD effects of acidosis and hypermagnesemia resembled acidosis alone. It is postulated that the final composition and crystal structure of bone are strongly influenced by HCO3- and Mg2+, and the effects are mediated by the combined influence on both osteoblastic bone formation and the growth of hydroxyapatite.

scholarly journals Effect of the suspension of Ag-incorporated TiO2 nanoparticles (Ag-TiO2 NPs) on certain growth, physiology and phytotoxicity parameters in spinach seedlings

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0244511
Author(s):  
Fernando Gordillo-Delgado ◽  
Jakeline Zuluaga-Acosta ◽  
Gonzalo Restrepo-Guerrero
Keyword(s):  
Particle Size ◽  
Titanium Dioxide Nanoparticles ◽  
Germination Rate ◽  
Sol Gel ◽  
Control Group ◽  
Human Consumption ◽  
X Ray Diffraction ◽  
X Ray ◽  
Growth Physiology ◽  
Powder Samples

In this work, the effect of the inoculation of silver-incorporated titanium dioxide nanoparticles (Ag-TiO2 NPs) in spinach seeds was evaluated on certain growth, physiology and phytotoxicity parameters of the plants. This is an important crop for human consumption with high nutritional value due to their low calorie and fat content, providing various vitamins and minerals, especially iron. These NPs were obtained by means of the sol-gel method and heat treatment; the resulting powder material was characterized using X-ray diffraction and scanning electron microscopy and the influence of these NPs on plants was measured by estimating the germination rate, monitoring morphological parameters and evaluating phytotoxicity. The photosynthetic activity of the spinach plants was estimated through the quantification of the Ratio of Oxygen Evolution (ROE) by the photoacoustic technique. Samples of TiO2 powder with particle size between 9 and 43 nm were used to quantify the germination rate, which served to determine a narrower size range between 7 and 26 nm in the experiments with Ag-TiO2 NPs; the presence of Ag in TiO2 powder samples was confirmed by energy-dispersive X-ray spectroscopy. The analysis of variance showed that the dependent variable (plant growth) could be affected by the evaluated factors (concentration and size) with significant differences. The statistical trend indicated that the application of the Ag-TiO2 NPs suspension of lowest concentration and smallest particle size could be a promoting agent of the growth and development of these plants. The inoculation with NPs of 8.3 nm size and lowest concentration was related to the highest average ROE value, 24.6 ± 0.2%, while the control group was 20.2 ± 0.2%. The positive effect of the Ag-TiO2 NPs treatment could be associated to the generation of reactive oxygen species, antimicrobial activity, increased biochemical attributes, enzymatic activity or improvements in water absorption.

Structure of Carbonated Hydroxyapatite Based on Rietveld Method

2008 ◽  
Vol 368-372 ◽  
pp. 1187-1189
Author(s):  
Xu Ran ◽  
Jun Guo Ran ◽  
Li Gou ◽  
Ji Yong Chen ◽  
Jiao Min Luo
Keyword(s):  
Crystal Structure ◽  
Sintering Temperature ◽  
Rietveld Method ◽  
Rietveld Analysis ◽  
Structure Parameters ◽  
X Ray Diffraction ◽  
Carbonated Hydroxyapatite ◽  
X Ray ◽  
Quantitative Results ◽  
Distortion Index

The crystalline structures of B-type carbonated hydroxyapatite (CHA) powders sintered at 700, 900 and 1100°C, respectively, were studied by Rietveld analysis of powder X-ray diffraction (XRD) data. A series of structure parameters, including lattice parameters (a and c), bond length and the distortion index of PO4 tetrahedron (Dind) were calculated by Rietveld method to characterize the fine structure of CHA. The broadening effect of XRD reflections was separated to calculate the micro-strain and crystalline size. The results showed that CHA become more stable with the increase of sintering temperature, but the CO3 2- is almost lost at temperature of 1100°C. The quantitative results about crystal structure of CHA based on crystalline structure simulated by Rietveld method are obtained.

Thiosemicarbazonate complexes with affinity for amyloid-β fibers: synthesis, characterization and biological studies

2019 ◽  
Vol 11 (19) ◽  
pp. 2527-2546 ◽  
Author(s):  
Arantxa Pino-Cuevas ◽  
Paula D Raposinho ◽  
Célia Fernandes ◽  
António Paulo ◽  
Ulrich Abram ◽  
...  
Keyword(s):  
Binding Affinity ◽  
Blood Brain Barrier ◽  
Free Ligand ◽  
Amyloid Β ◽  
Molecular Structures ◽  
X Ray Diffraction ◽  
X Ray ◽  
Biological Studies ◽  
Similar Affinity

Aim: Obtain radioimages of amyloid-β fibers using 99mTc-complexes. Methodology: Tridentate thiosemicarbazone and thiocarbonohydrazone ligands containing fragments (stilbene, azobenzene, benzothiazole or benzoxazole) with affinity for amyloid-ß fibers and its Re(I) complexes have been prepared. The molecular structures of several ligands and complexes were determined by x-ray diffraction. Binding affinity studies toward Aß1-42 fibers were performed for the ligands and Re(I) complexes. The ability of formation of some 99mTc(I) complexes, their biodistribution and in vivo stability have been established. Results & conclusion: Complexes of stilbene and benzothiazole thiosemicarbazonates show similar affinity for amyloid-β fibers to the free ligand. These 99mTc complexes present a reasonable in vivo stability and a low capability to cross the blood–brain barrier although not sufficient to brain amyloid imaging.

Histologic and Ultrastructural Analysis in a Case of Massive Failure of Zirconia Ball Head

2002 ◽  
Vol 12 (4) ◽  
pp. 388-393
Author(s):  
G. Maccauro ◽  
L. Petrella ◽  
L. Proietti ◽  
V. De Santis ◽  
C. Piconi
Keyword(s):  
Zirconium Oxide ◽  
Internal Surface ◽  
Giant Cells ◽  
Sem Analysis ◽  
Ultrastructural Analysis ◽  
Periprosthetic Tissue ◽  
X Ray ◽  
Internal Part ◽  
Scanning Electron

The in vivo histological and ultrastructural analysis of periprosthetic tissue collected at time of revision surgery for failure of a 28 mm yttria-tetragonal zirconium oxide polycrystal (YTZP) ball head, 2 years after implantation is reported. Histologic analysis showed Zirconia particles, rounded to polygonal ranging from 2μm to more than 10μm intra or extra cellular in a stroma without vessels with few giant cells. Scanning electron microscopy (SEM) and energy dispersive X-ray analysis (EDAX) showed the different shape from rounded to polygonal of Zirconia. SEM analysis of the internal part of Zirconia ball head showed the presence of different fracture lines especially in the internal surface

In Vivo Biological Behavior of Calcium Phosphate Cements with Different Ca/P Ratio

2008 ◽  
Vol 396-398 ◽  
pp. 237-240
Author(s):  
Sybele Saska ◽  
N.S. Nunes ◽  
Emelly Aveiro ◽  
Cristina A.C. Pavan ◽  
S.H. Santagneli ◽  
...  
Keyword(s):  
Thermogravimetric Analysis ◽  
31P Nmr ◽  
Phosphate Buffer Solution ◽  
Biological Behavior ◽  
X Ray Diffraction ◽  
Buffer Solution ◽  
X Ray ◽  
Calcium Phosphate Cements ◽  
Subcutaneous Tissues

Bioceramics with different Ca/P ratio were prepared from a mechanical mixture of NaPO3, CaCO3, Ca(OH)2 and phosphate buffer solution and implanted in rats subcutaneous tissues. The cements were characterized by Thermogravimetric analysis (TG-TDA), X-ray diffraction and 31P-NMR. The implant sites were excised after 1, 4 and 16 weeks, fixed, dehydrated, included in paraffin wax for serial cutting and examined under the light transmitted microscope. They were biocompatible and biodegradable when implanted in rat subcutaneous. None of the materials induced ectopic osteogenesis. According to the results, the studied materials seem to be able for manufacturing reabsorbable bone implants.

Compositional and architectural variation in urinary calculi from nucleus to periphery: an integrated IR spectroscopy, scanning electron microscopy and powder X-ray diffraction approach

2015 ◽  
Vol 48 (6) ◽  
pp. 1794-1804 ◽  
Author(s):  
Paramita Chatterjee ◽  
Samiran Pramanik ◽  
Alok Kumar Mukherjee
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Kidney Stones ◽  
Urinary Calculi ◽  
X Ray Diffraction ◽  
Carbonated Hydroxyapatite ◽  
X Ray ◽  
The Core ◽  
Different Parts ◽  
Scanning Electron

A combination of IR spectroscopy, scanning electron microscopy (SEM) and powder X-ray diffraction has been used to analyze the compositional and architectural variation across the different parts (core, middle and outer layers) of five human urinary calculi (KS1–KS5) from eastern India. Rietveld quantitative phase analysis using X-ray powder diffraction revealed that the composition of the core regions in KS1–KS3 and KS5 is exclusively whewellite, whereas in KS4 it is a mixture of whewellite (84.5 wt%) and carbonated hydroxyapatite (15.5 wt%). While one of the renal stones, KS1, is composed of only whewellite in all three regions, a distinct variation in phase composition from the core towards the periphery has been observed in KS2–KS5. A drastic change in phase composition has been noted in KS5, with the major constituent phases in the core, middle and outer layers as whewellite (100.0 wt%), anhydrous uric acid (60.7 wt%) and carbonated hydroxyapatite (69.6 wt%), respectively. The crystallite size of whewellite in different parts of the kidney stones varies between 91 (1) and 167 (1) nm, while the corresponding sizes of the anhydrous uric acid in KS5 and carbonated hydroxyapatite in KS3 are 107 (1) and 18 (1)–20 (1) nm, respectively. SEM images of the kidney stones showed different levels of organization, resulting from an agglomeration of crystallites with diverse shapes and sizes.

Strategy of an in vivo Study of the Evolution of Drugs via X-Ray Diffraction with Synchrotron Radiation

2018 ◽  
Vol 82 (7) ◽  
pp. 895-897
Author(s):  
M. R. Sharafutdinov ◽  
B. P. Tolochko ◽  
K. E. Kuper ◽  
A. I. Ancharov
Keyword(s):  
Synchrotron Radiation ◽  
In Vivo Study ◽  
X Ray Diffraction ◽  
X Ray
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