Cytocompatiblity of Ceramic Nanoparticles to Various Types of Cells

2016 ◽  
Vol 696 ◽  
pp. 234-237
Author(s):  
Shigeaki Abe ◽  
Nobuki Iwadera ◽  
Mami Mutoh ◽  
Tomohiko Katsurayama ◽  
Shino Morimoto ◽  
...  
Keyword(s):  
Particle Size ◽  
Fluorescence Microscopy ◽  
Cell Viability ◽  
Copper Oxide ◽  
Cuo Nanoparticles ◽  
Lung Epithelial Cells ◽  
Ceramic Nanoparticles ◽  
Lung Epithelial ◽  
Confocal Fluorescence ◽  
Different Types

In this study, we investigated the cytocompatibility of ceramic nanoparticles on different types of cells. All ceramics nanoparticles investigated in this study except Copper oxide (CuO) exhibited good cytocompatibility and cell viability (90% or more) even at 20 ppm concentration. In contrast, CuO nanoparticles caused cell inflammation, and their effect depended on their particle size. Confocal fluorescence microscopy measurements indicated that some particles had penetrated into the cells. These results indicate that except CuO nanoparticles, all other ceramic nanoparticles reported herein exhibited excellent cytocompatibility even for lung epithelial cells.

scholarly journals Size-dependent cytotoxicity of copper oxide nanoparticles in lung epithelial cells

2016 ◽  
Vol 3 (2) ◽  
pp. 365-374 ◽  
Author(s):  
Amaraporn Wongrakpanich ◽  
Imali A. Mudunkotuwa ◽  
Sean M. Geary ◽  
Angie S. Morris ◽  
Kranti A. Mapuskar ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Copper Oxide ◽  
Copper Oxide Nanoparticles ◽  
Cuo Nanoparticles ◽  
Lung Epithelial Cells ◽  
Oxide Nanoparticles ◽  
Size Dependent ◽  
Lung Epithelial

The increasing use of copper oxide (CuO) nanoparticles (NPs) in medicine and industry demands an understanding of their potential toxicities.

scholarly journals Cytotoxic Effects of Air Freshener Biocides in Lung Epithelial Cells

2013 ◽  
Vol 8 (9) ◽  
pp. 1934578X1300800
Author(s):  
Jung-Taek Kwon ◽  
Mimi Lee ◽  
Gun-Baek Seo ◽  
Hyun-Mi Kim ◽  
Ilseob Shim ◽  
...  
Keyword(s):  
Dna Damage ◽  
Epithelial Cells ◽  
Cell Viability ◽  
Lung Epithelial Cells ◽  
Ros Generation ◽  
Dependent Manner ◽  
Cytotoxic Effects ◽  
Lung Epithelial ◽  
Dose Dependent ◽  
A549 Lung

This study evaluated the cytotoxicity of mixtures of citral (CTR) and either benzisothiazolinone (BIT, Mix-CTR-BIT) or triclosan (TCS, Mix-CTR-TCS) in human A549 lung epithelial cells. We investigated the effects of various mix ratios of these common air freshener ingredients on cell viability, cell proliferation, reactive oxygen species (ROS) generation, and DNA damage. Mix-CTR-BIT and Mix-CTR-TCS significantly decreased the viability of lung epithelial cells and inhibited cell growth in a dose-dependent manner. In addition, both mixtures increased ROS generation, compared to that observed in control cells. In particular, cell viability, growth, and morphology were affected upon increase in the proportion of BIT or TCS in the mixture. However, comet analysis showed that treatment of cells with Mix-CTR-BIT or Mix-CTR-TCS did not increase DNA damage. Taken together, these data suggested that increasing the content of biocides in air fresheners might induce cytotoxicity, and that screening these compounds using lung epithelial cells may contribute to hazard assessment.

Evaluation of the Biocompatibility of Ceramic Nanoparticles with A549 Lung Epithelial Cells

2016 ◽  
Vol 8 (4) ◽  
pp. 310-315 ◽  
Author(s):  
Nozomu Kiyama ◽  
Shigeaki Abe ◽  
Shuichi Yamagata ◽  
Yasuhiro Yoshida ◽  
Junichiro Iida
Keyword(s):  
Epithelial Cells ◽  
Lung Epithelial Cells ◽  
Ceramic Nanoparticles ◽  
Lung Epithelial ◽  
A549 Lung

Preparation of Copper Oxide (CuO) Nanoparticles and their Bactericidal Activity

2012 ◽  
pp. 326-332
Author(s):  
F. M. Al-Marzouki ◽  
O. A. Al-Hartomy ◽  
M. A. Shah
Keyword(s):  
Particle Size ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Copper Oxide ◽  
Bactericidal Activity ◽  
Copper Powder ◽  
Low Temperatures ◽  
Cost Effective ◽  
Cuo Nanoparticles ◽  
Uniform Particle Size

Single crystalline nanoparticles of copper oxide (CuO) having almost uniform particle size of ~40±10nm have been synthesized by a facile and versatile route. The technique employed is free from toxic solvents, organics, and amines, and is based on a simple reaction of copper powder and de-ionized water (DI) at very low temperatures of 180oC. The morphology, chemical composition, and crystalline structure of the nanoparticles were carefully investigated by the various characterization techniques. Besides simplicity, the advantages of producing nanoparticles by this method are that it is easeful, flexible, fast, cost effective, and pollution free. The synthesized nanoparticles are under investigations for various applications including their antibacterial activity.

Genotoxic potential of copper oxide nanoparticles in human lung epithelial cells

2010 ◽  
Vol 396 (2) ◽  
pp. 578-583 ◽  
Author(s):  
Maqusood Ahamed ◽  
Maqsood A. Siddiqui ◽  
Mohd J. Akhtar ◽  
Iqbal Ahmad ◽  
Aditya B. Pant ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Copper Oxide ◽  
Human Lung ◽  
Copper Oxide Nanoparticles ◽  
Lung Epithelial Cells ◽  
Oxide Nanoparticles ◽  
Genotoxic Potential ◽  
Lung Epithelial ◽  
Human Lung Epithelial Cells

Preparation and Stability Characterization of Copper Oxide Nanofluid by Two Step Method

2015 ◽  
Vol 832 ◽  
pp. 139-143 ◽  
Author(s):  
A.L. Subramaniyan ◽  
Arun Kumar ◽  
Sethupathi ◽  
T. Sorna Kumar ◽  
R. Ilangovan
Keyword(s):  
Particle Size ◽  
Copper Oxide ◽  
Copper Chloride ◽  
Sol Gel ◽  
Cuo Nanoparticles ◽  
Step Method ◽  
Sem Image ◽  
Particle Size Analyzer ◽  
Negative Zeta Potential

Copper oxide (CuO) nanofluids are prepared by two step method. CuO nanoparticles are prepared by sol gel method with Copper chloride as precursor and NaOH as a reducing agent. The prepared CuO nanoparticles are characterized by XRD, SEM and Particle size analyzer. XRD measurements reveal a grain size of 72nm .SEM image reveals an inhomogenous mixture of particles from 500 nm to 3500 nm with irregular morphology and few traces of CuO cones.. The particle size distribution of CuO particles is in the range of 1000-1600nm which is in accordance with the SEM results.CuO –Ethylene Glycol nanofluids are prepared by two step method by ultrasonication of obtained CuO particles.The stability of CuO nanofluid is given with a negative zeta potential of-22.5 mv.

scholarly journals Puerarin Inhibits Ferroptosis and Inflammation of Lung Injury Caused by Sepsis in LPS Induced Lung Epithelial Cells

2021 ◽  
Author(s):  
Baiye Xu ◽  
Haidao Wang ◽  
Zhen Chen
Keyword(s):  
Epithelial Cells ◽  
Lung Injury ◽  
Cell Viability ◽  
A549 Cell ◽  
A549 Cells ◽  
Total Iron ◽  
Lung Epithelial Cells ◽  
Expression Level ◽  
Lung Epithelial ◽  
Related Proteins

Abstract Background: Ferroptosis is a new type of programmed cell death, which plays an important role in lung injury caused by sepsis. Studies have reported that Puerarin (Pue) can treat lung injury caused by sepsis in children, but whether it plays a role by regulating iron death has not been reported.Methods: LPS induced human alveolar epithelial cell A549 to form a model of lung injury caused by sepsis. MTT detected the effect of Pue on A549 cell viability and the effect of Pue on LPS-induced A549 cell viability. The effects of Pue on LPS-induced inflammatory cytokines TNF-α, IL-8, IL-1β in A549 cells were determined by ELISA assay. The expression level of MDA was detected by TBARS colorimetric quantitative detection kit. GSH kit was used to detect the expression of GSH in cells. The iron kit detected the total iron level and the expression level of ferric divalent ions in the cells. DCFH-DA fluorescent probe was used to detect ROS levels. Western blot was used to detect the expression of ferroptosis-related proteins in cells. Results: Pue alleviated LPS-induced injury and inflammatory response in A549 cells, and Pue reduced the expression of ROS, MDA and GSH in LPS-induced A549 cells. In addition, Pue reduced total iron levels and ferrous ion levels in LPS-induced A549 cells, and decreased the expression of iron ferroptosis-related proteins. Conclusion: Puerarin inhibited ferroptosis and inflammation of lung injury caused by sepsis in children in LPS induced lung epithelial cells.

Sign in / Sign up

Export Citation Format

Share Document