Antibacterial Properties of Silver Nanoparticles Synthesized Using Piper betle L. Leaf Extract

2021 ◽  
Vol 1020 ◽  
pp. 236-242
Author(s):  
Tien Hieu Vu ◽  
Van Huan Bui ◽  
Ngoc Thang Nguyen
Keyword(s):  
Silver Nanoparticles ◽  
Pathogenic Bacteria ◽  
Leaf Extract ◽  
Biomedical Application ◽  
Antibacterial Properties ◽  
Silver Ions ◽  
Infrared Spectrometry ◽  
Piper Betle ◽  
Plasmon Band ◽  
Biosynthesized Agnps

The green synthesis of silver nanoparticles (AgNPs) using herbal plants has gained much attention due to their potential widespread applications, especially in biomedical application to control pathogenic microbes. The aim of our study was to evaluate the antibacterial properties of synthesized AgNPs using aqueous leaf extract of Piper betle L., an important medicinal plant. The AgNPs were identified by UV-Visible spectrometry (UV-Vis), X-ray diffraction (XRD), transmission electron microscopy (TEM) and Fourier transform infrared spectrometry (FT-IR). The presence of surface plasmon band around 420-460 nm indicated AgNPs formation. Spherical nature, unique size-distribution and crystal structure of the AgNPs with diameter around 10-20 nm were affirmed by TEM and XRD analyses. The FTIR measurements showed the presence of bioactive compounds in the extract responsible for the efficient reduction of silver ions and stabilization of the AgNPs. The results from the antimicrobial assays suggested that the biosynthesized AgNPs were potent against pathogenic bacteria including Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus.

scholarly journals Green Biosynthesis of Silver Nanoparticles Using Leaf Extract of Carissa carandas L. and Their Antioxidant and Antimicrobial Activity against Human Pathogenic Bacteria

Biomolecules ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 299
Author(s):  
Reetika Singh ◽  
Christophe Hano ◽  
Gopal Nath ◽  
Bechan Sharma
Keyword(s):  
Silver Nanoparticles ◽  
Pathogenic Bacteria ◽  
Leaf Extract ◽  
Room Temperature ◽  
Xrd Analysis ◽  
Antibacterial Activities ◽  
Effect Of Temperature ◽  
Human Pathogenic Bacteria ◽  
Carissa Carandas ◽  
Antioxidant And Antimicrobial Activity

Carissa carandas L. is traditionally used as antibacterial medicine and accumulates many antioxidant phytochemicals. Here, we expand this traditional usage with the green biosynthesis of silver nanoparticles (AgNPs) achieved using a Carissa carandas L. leaf extract as a reducing and capping agent. The green synthesis of AgNPs reaction was carried out using 1mM silver nitrate and leaf extract. The effect of temperature on the synthesis of AgNPs was examined using room temperature (25 °C) and 60 °C. The silver nanoparticles were formed in one hour by stirring at room temperature. In this case, a yellowish brown colour was developed. The successful formation of silver nanoparticles was confirmed by UV–Vis, Fourier transform infrared (FT-IR) and X-ray diffraction (XRD) analysis. The characteristic peaks of the UV-vis spectrum and XRD confirmed the synthesis of AgNPs. The biosynthesised AgNPs showed potential antioxidant activity through DPPH assay. These AgNPs also exhibited potential antibacterial activity against human pathogenic bacteria. The results were compared with the antioxidant and antibacterial activities of the plant extract, and clearly suggest that the green biosynthesized AgNPs can constitute an effective antioxidant and antibacterial agent.

Immobilization of Silver Nanoparticles on Chitosan-Coated Silica-Gel-Beads and the Antibacterial Activity

2021 ◽  
Vol 892 ◽  
pp. 36-42
Author(s):  
Muhammad Iqbal Hidayat ◽  
Muhammad Adlim ◽  
Ilham Maulana ◽  
Muhammad Zulfajri
Keyword(s):  
Silver Nanoparticles ◽  
Silica Gel ◽  
Low Cost ◽  
Antibacterial Properties ◽  
Chitosan Film ◽  
Silver Ions ◽  
Filter Material ◽  
Silver Ion ◽  
Gel Beads ◽  
Metal Colloid

Silver nanoparticles (Ag0) have attracted the most attention due to their broad antimicrobial application and outstanding activity. The silver nanoparticles are usually in colloidal form, then immobilization the colloid onto solid support is still interesting to explore. In this work, a new method for silver colloidal nanoparticle immobilization on silica gel beads (SiG), which was then symbolized as Ag0-[chi-SiG] was conducted and characterized successfully. The finding proved that SiG must be coated with three chitosan film layers to give stable support for silver nanoparticles. This coating method caused the chitosan completely covered SiG, and the chitosan film provides coordination bonding for silver ions. The most appropriate solvent for silver ion impregnation on the surface of chi-SiG is methanol compared to other solvents. Tungsten lamp as the photo-irradiation, which is low cost and environmentally friendly has been proven effective for silver ion reduction, as shown by silver metal colloid UV-Vis surface plasmon resonance at 400-700 nm. Ag0-[chi-SiG] showed the antibacterial properties of inhibiting the growth Staphylococcus aureus and Escherichia coli; then it provides the potential application for antibacterial filter material. According to the weight comparison between antibacterial standard and Ag content, then Ag0-[chi-SiG] has two and five times higher of exhibiting zone for each bacteria.

scholarly journals Synthesis of Silver Nano Particles from Adansonia digitata Leaf Extract and Its Antimicrobial Properties

2020 ◽  
pp. 10-22
Author(s):  
A. O. Akintola ◽  
A. M. Azeez ◽  
B. D. Kehinde ◽  
I. C. Oladipo
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Aspergillus Niger ◽  
Pathogenic Bacteria ◽  
Leaf Extract ◽  
Biomedical Application ◽  
Nano Particles ◽  
Diffusion Method ◽  
Adansonia Digitata ◽  
Inhibition Zones

Silver nano particles (AgNPs) were green synthesized using Adansonia digitata leaf extract. The synthesized silver nano particles were characterized in terms of synthesis, size, shape, morphology and capping functionalities by UV-Visible Spectroscopy, Scanning Electron Microscopy (SEM) and Fourier Transform Infrared Spectroscopy (FTIR). Antimicrobial activity of the synthesized silver nano particles was investigated by well diffusion method. The antibacterial activity of the nano particle was studied against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeurigunosa, Salmonella typhi and Klebsiella pneumonae while the antifungal activity was studied against Candida albicans, Aspergillus niger, Penicillum notatum and Rhizopus stolomifer. The synthesized AgNPs was active against all the studied microorganisms. Staphylococcus aureus was the most susceptible bacterium (inhibition zones ranging from 12.00 to 28.00 mm, MIC: 30 µl, MBC: 50 µl) while Aspergillus niger was the most susceptible fungi (inhibition zones ranging from 10.00 to 18.00 mm, MIC: 90 µl, MFC: 120 µl. In conclusion the synthesized silver nanoparticles was found to have antimicrobial activity against the pathogenic bacteria and fungi tested and hence has a great potential in biomedical application for the treatment of microbial infections.

scholarly journals Microwave-Assisted Green Synthesis of Silver Nanoparticles Using Juglans regia Leaf Extract and Evaluation of Their Physico-Chemical and Antibacterial Properties

Antibiotics ◽  
2018 ◽  
Vol 7 (3) ◽  
pp. 68 ◽  
Author(s):  
Mahsa Eshghi ◽  
Hamideh Vaghari ◽  
Yahya Najian ◽  
Mohammad Najian ◽  
Hoda Jafarizadeh-Malmiri ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Juglans Regia ◽  
Antibacterial Properties ◽  
Infrared Analysis ◽  
Ag Nps ◽  
Stabilizing Agents ◽  
Agno3 Solution ◽  
Transmission Electron ◽  
Vis Spectroscopy

Silver nanoparticles (Ag NPs) were synthesized using Juglans regia (J. regia) leaf extract, as both reducing and stabilizing agents through microwave irradiation method. The effects of a 1% (w/v) amount of leaf extract (0.1–0.9 mL) and an amount of 1 mM AgNO3 solution (15–25 mL) on the broad emission peak (λmax) and concentration of the synthesized Ag NPs solution were investigated using response surface methodology (RSM). Fourier transform infrared analysis indicated the main functional groups existing in the J. regia leaf extract. Dynamic light scattering, UV-Vis spectroscopy and transmission electron microscopy were used to characterize the synthesized Ag NPs. Fabricated Ag NPs with the mean particle size and polydispersity index and maximum concentration and zeta potential of 168 nm, 0.419, 135.16 ppm and −15.6 mV, respectively, were obtained using 0.1 mL of J. regia leaf extract and 15 mL of AgNO3. The antibacterial activity of the fabricated Ag NPs was assessed against both Gram negative (Escherichia coli) and positive (Staphylococcus aureus) bacteria and was found to possess high bactericidal effects.

Enhancement of antibacterial properties of silver nanoparticles–ceftriaxone conjugate through Mukia maderaspatana leaf extract mediated synthesis

2015 ◽  
Vol 121 ◽  
pp. 135-141 ◽  
Author(s):  
Muthukumar Harshiny ◽  
Manickam Matheswaran ◽  
Gangasalam Arthanareeswaran ◽  
Shanmugam Kumaran ◽  
Shanmuganathan Rajasree
Keyword(s):  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Antibacterial Properties

scholarly journals Antimicrobial Potential of Silver Nanoparticles Synthesized Using Medicinal Herb Coptidis rhizome

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2268 ◽  
Author(s):  
Garima Sharma ◽  
Ju-Suk Nam ◽  
Ashish Sharma ◽  
Sang-Soo Lee
Keyword(s):  
Silver Nanoparticles ◽  
Aqueous Extract ◽  
Inductively Coupled Plasma ◽  
Antimicrobial Property ◽  
Spherical Shape ◽  
Silver Ions ◽  
Average Diameter ◽  
Xrd Analysis ◽  
Visible Absorption ◽  
Biosynthesized Agnps

Coptidis rhizome contains several alkaloids that are bioactive agents of therapeutic value. We propose an eco-friendly method to synthesize biocompatible silver nanoparticles (AgNPs) using the aqueous extract of Coptidis rhizome. Silver ions were reduced to AgNPs using the aqueous extract of Coptidis rhizome, indicating that Coptidis rhizome can be used for the biosynthesis of AgNPs. The time and the concentration required for conversion of silver ions into AgNPs was optimized using UV-absorbance spectroscopy and inductively coupled plasma spectroscopy (ICP). Biosynthesized AgNPs showed a distinct UV-Visible absorption peak at 420 nm. ICP analysis showed that the time required for the completion of biosynthesis was around 20 min. Microscopic images showed that nanoparticles synthesized were of spherical shape and the average diameter of biosynthesized AgNPs was less than 30 nm. XRD analysis also confirmed the size of AgNps and revealed their crystalline nature. The interaction of AgNPs with phytochemicals present in Coptidis rhizome extract was observed in FTIR analysis. The antimicrobial property of AgNPs was evaluated using turbidity measurements. Coptidis rhizome-mediated biosynthesized AgNPs showed significant anti-bacterial activities against Escherichia coli and Staphylococcus aureus that are commonly involved in various types of infections, indicating their potential as an effective anti-bacterial agent.

scholarly journals Effect of feed supplementation with biosynthesized silver nanoparticles using leaf extract of Morus indica L. V1 on Bombyx mori L. (Lepidoptera: Bombycidae)

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Sudip Some ◽  
Onur Bulut ◽  
Kinkar Biswas ◽  
Anoop Kumar ◽  
Anupam Roy ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Bombyx Mori ◽  
Leaf Extract ◽  
Silver Ions ◽  
Average Diameter ◽  
Face Centered Cubic ◽  
Tem Analysis ◽  
Beneficial Effects ◽  
Selected Area Electron Diffraction ◽  
Face Centered

Abstract Herein, we report the synthesis of silver nanoparticles (AgNPs) by a green route using the aqueous leaf extract of Morus indica L. V1. The synthesized AgNPs exhibited maximum UV-Vis absorbance at 460 nm due to surface plasmon resonance. The average diameter (~54 nm) of AgNPs was measured from HR-TEM analysis. EDX spectra also supported the formation of AgNPs, and negative zeta potential value (−14 mV) suggested its stability. Moreover, a shift in the carbonyl stretching (from 1639 cm−1 to 1630 cm−1) was noted in the FT-IR spectra of leaf extract after AgNPs synthesis which confirm the role of natural products present in leaves for the conversion of silver ions to AgNPs. The four bright circular rings (111), (200), (220) and (311) observed in the selected area electron diffraction pattern are the characteristic reflections of face centered cubic crystalline silver. LC-MS/MS study revealed the presence of phytochemicals in the leaf extract which is responsible for the reduction of silver ions. MTT assay was performed to investigate the cytotoxicity of AgNPs against two human cell lines, namely HepG2 and WRL-68. The antibacterial study revealed that MIC value of the synthesized AgNPs was 80 µg/ml against Escherichia coli K12 and Staphylococcus aureus (MTCC 96). Finally, the synthesized AgNPs at 10 µg/ml dosages showed beneficial effects on the survivability, body weights of the Bombyx mori L. larvae, pupae, cocoons and shells weights via enhancing the feed efficacy.

scholarly journals Degradation of Methylene Blue Using Biologically Synthesized Silver Nanoparticles

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
M. Vanaja ◽  
K. Paulkumar ◽  
M. Baburaja ◽  
S. Rajeshkumar ◽  
G. Gnanajobitha ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Nitrate Solution ◽  
Reaction Medium ◽  
Silver Ions ◽  
Silver Nitrate Solution ◽  
X Ray ◽  
Synthesis Of Nanoparticles ◽  
Aqueous Leaf Extract

Nowadays plant mediated synthesis of nanoparticles has great interest and achievement due to its eco-benign and low time consuming properties. In this study silver nanoparticles were successfully synthesized by usingMorinda tinctorialeaf extract under different pH. The aqueous leaf extract was added to silver nitrate solution; the color of the reaction medium was changed from pale yellow to brown and that indicates reduction of silver ions to silver nanoparticles. Thus synthesized silver nanoparticles were characterized by UV-Vis spectrophotometer. Dispersity and morphology was characterized by scanning electron microscope (SEM); crystalline nature and purity of synthesized silver nanoparticles were revealed by X-ray diffraction (XRD) and energy dispersive X-ray spectroscopy (EDX). FTIR spectrum was examined to identify the effective functional molecules responsible for the reduction and stabilization of silver nanoparticles synthesized by leaf extract. The photocatalytic activity of the synthesized silver nanoparticles was examined by degradation of methylene blue under sunlight irradiation. Green synthesized silver nanoparticles were effectively degrading the dye nearly 95% at 72 h of exposure time.

scholarly journals A Green Approach to Synthesize Silver Nanoparticles in Starch-co-Poly(acrylamide) Hydrogels by Tridax procumbens Leaf Extract and Their Antibacterial Activity

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Siraj Shaik ◽  
Madhusudana Rao Kummara ◽  
Sudhakar Poluru ◽  
Chandrababu Allu ◽  
Jaffer Mohiddin Gooty ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Silver Ions ◽  
Inhibition Zone ◽  
Zone Method ◽  
Green Approach ◽  
Tridax Procumbens ◽  
Poly Acrylamide

A series of starch-co-poly(acrylamide) (starch-co-PAAm) hydrogels were synthesized by employing free radical redox polymerization. A novel green approach, Tridax procumbens (TD) leaf extract, was used for reduction of silver ions (Ag+) into silver nanoparticles in the starch-co-PAAm hydrogel network. The formation of silver nanoparticles was confirmed by UV-visible spectroscopy (UV-Vis), thermogravimetric analysis (TGA), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (X-RD) studies. 22% of weight loss difference between hydrogel and silver nanocomposite hydrogel (SNCH) clearly indicates the formation of silver nanoparticles by TGA. TEM images indicate the successful incorporation of silver nanoparticles ranging from 5 to 10 nm in size and spherical in shape with a narrow size distribution. These developed SNCHs were used to study the antibacterial activity by inhibition zone method against gram-positive and gram-negative bacteria such as Bacillus and Escherichia coli. The results indicated that these SNCHs can be used potentially for biomedical applications.

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