Immobilization of Silver Nanoparticles on Chitosan-Coated Silica-Gel-Beads and the Antibacterial Activity

2021 ◽  
Vol 892 ◽  
pp. 36-42
Author(s):  
Muhammad Iqbal Hidayat ◽  
Muhammad Adlim ◽  
Ilham Maulana ◽  
Muhammad Zulfajri
Keyword(s):  
Silver Nanoparticles ◽  
Silica Gel ◽  
Low Cost ◽  
Antibacterial Properties ◽  
Chitosan Film ◽  
Silver Ions ◽  
Filter Material ◽  
Silver Ion ◽  
Gel Beads ◽  
Metal Colloid

Silver nanoparticles (Ag0) have attracted the most attention due to their broad antimicrobial application and outstanding activity. The silver nanoparticles are usually in colloidal form, then immobilization the colloid onto solid support is still interesting to explore. In this work, a new method for silver colloidal nanoparticle immobilization on silica gel beads (SiG), which was then symbolized as Ag0-[chi-SiG] was conducted and characterized successfully. The finding proved that SiG must be coated with three chitosan film layers to give stable support for silver nanoparticles. This coating method caused the chitosan completely covered SiG, and the chitosan film provides coordination bonding for silver ions. The most appropriate solvent for silver ion impregnation on the surface of chi-SiG is methanol compared to other solvents. Tungsten lamp as the photo-irradiation, which is low cost and environmentally friendly has been proven effective for silver ion reduction, as shown by silver metal colloid UV-Vis surface plasmon resonance at 400-700 nm. Ag0-[chi-SiG] showed the antibacterial properties of inhibiting the growth Staphylococcus aureus and Escherichia coli; then it provides the potential application for antibacterial filter material. According to the weight comparison between antibacterial standard and Ag content, then Ag0-[chi-SiG] has two and five times higher of exhibiting zone for each bacteria.

scholarly journals Study of Antibacterial Properties of Ziziphus mauritiana based Green Synthesized Silver Nanoparticles against Various Bacterial Strains

2020 ◽  
Vol 12 (4) ◽  
pp. 1484 ◽  
Author(s):  
M. Asimuddin ◽  
Mohammed Rafi Shaik ◽  
Neeshat Fathima ◽  
M. Shaistha Afreen ◽  
Syed Farooq Adil ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Metallic Nanoparticles ◽  
Low Cost ◽  
Preliminary Investigation ◽  
Antibacterial Properties ◽  
Silver Ions ◽  
Ziziphus Mauritiana ◽  
Bacterial Strains ◽  
Vis Spectroscopy ◽  
Ft Ir

Due to their low cost and environmentally friendly nature, plant extracts based methods have gained significant popularity among researchers for the synthesis of metallic nanoparticles. Herein, green synthesis of silver nanoparticles was performed using the aqueous solution of Ziziphus mauritiana leaves extract (ZM-LE) as a bio-reducing agent. The as-obtained silver nanoparticles were characterized by using UV-Vis spectroscopy, XRD (X-ray diffraction), TEM (transmission electron microscopy), and FT-IR (Fourier-transform infrared spectroscopy). In addition, the effects of the concentrations of the leaves extract, silver nitrate, and the temperature on the preparation of nanoparticles were also investigated. In order to determine the nature of secondary metabolites present in leaves extract, a preliminary investigation of phytoconstituents was carried out using different methods including Folin-Ciocalteu and AlCl3 methods. The results have indicated the presence of a considerable amount of phenolic and flavonoid contents in the leaves extract, which are believed to be responsible for the reduction of silver ions and stabilization of resulting nanoparticles. Indeed, the FT-IR spectrum of silver nanoparticles also confirmed the presence of residual phytomolecules of leaves extract as stabilizing ligands on the surface of nanoparticles. The antibacterial properties of as-obtained silver nanoparticles were tested against various bacterial strains including Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Bacillus subtilis. The nanoparticles strongly inhibited the growth of S. aureus with a minimum inhibitory concentration (MIC) of 2.5 μg/ml and moderately inhibited the growth of E. coli with a MIC of 5 μg/ml.

scholarly journals Printable Resin Modified by Grafted Silver Nanoparticles for Preparation of Antifouling Microstructures with Antibacterial Effect

Polymers ◽  
2021 ◽  
Vol 13 (21) ◽  
pp. 3838
Author(s):  
Hazem Idriss ◽  
Roman Elashnikov ◽  
Silvie Rimpelová ◽  
Barbora Vokatá ◽  
Petr Haušild ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Effect ◽  
Low Cost ◽  
Antibacterial Properties ◽  
Silver Ions ◽  
Polymerization Process ◽  
Resin Matrix ◽  
Bacterial Strains ◽  
Ion Release ◽  
3D Printed

The usage of three-dimensional (3D) printed materials in many bioapplications has been one of the fastest-growing sectors in the nanobiomaterial industry in the last couple of years. In this work, we present a chemical approach for grafting silver nanoparticles (AgNPs) into a resin matrix, which is convenient for 3D printing. In this way, the samples can be prepared and are able to release silver ions (Ag+) with excellent antibacterial effect against bacterial strains of E. coli and S. epidermidis. By the proposed process, the AgNPs are perfectly mixed and involved in the polymerization process and their distribution in the matrix is homogenous. It was also demonstrated that this approach does not affect the printing resolution and the resin is therefore suitable for the construction of microstructures enabling controlled silver ion release and antifouling properties. At the same time the physical properties of the material, such as viscosity and elasticity modulus are preserved. The described approach can be used for the fabrication of facile, low-cost 3D printed resin with antifouling-antibacterial properties with the possibility to control the release of Ag+ through microstructuring.

Practical Method to Synthesize Silver Nanoparticles for Electro-Conductive Inkjet Inks in Aqueous Medium

2012 ◽  
Vol 441 ◽  
pp. 356-359
Author(s):  
Jun Natsuki ◽  
Takao Abe
Keyword(s):  
Silver Nanoparticles ◽  
Reduction Method ◽  
Low Cost ◽  
Silver Ions ◽  
Practical Method ◽  
Water Soluble ◽  
High Yield ◽  
Environment Protection ◽  
Uniform Particles ◽  
Short Time

This paper describes a new method to prepare silver nanoparticles useful for printing electronic circuits. We have tried to develop a silver nitrate reduction method, with which a certain reducing agent has played an important role in the reduction of silver ions in an aqueous solution. The reduction has occurred rapidly at room temperature and the silver particles have been separated very easily from the solution in a short time. In this process, any organic solvent has not been used and separation of silver nanoparticles has not been complicated. All chemicals used in this study have been water-soluble. Small and relatively uniform particles of a diameter lower than 10 nm can be obtained with high purity and high yield. These can result in low cost, safe treatment and good for environment protection. This method of producing colloidal silver nanoparticles will find practical use in electronics applications.

Characterization and antibacterial properties of Eriobotrya japonica extract loaded silver-nanoparticles

2020 ◽  
Vol 16 ◽  
Author(s):  
Arfaa Sajid ◽  
Qaisar Manzoor ◽  
Anam Sajid ◽  
Muhammad Imran ◽  
Shanza Khalid ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Silver Ions ◽  
Eriobotrya Japonica ◽  
X Ray Diffraction ◽  
Effective Utilization ◽  
E Coli ◽  
Elongated Nanoparticles

Background:: Currently, developing methods for the formation of nanoparticles with antimicrobial properties based on green chemistry are the research hotspots. In this research green biosynthesis of Eriobotrya japonica extract loaded silver nanoparticles and their characterization were the main objectives to achieve. Methods:: Green synthesis of E. japonica leaves extract-loaded silver nanoparticles (AgNPs) was carried out and its effect on bacterial growth was examined. The reduction of silver ions in solution was observed using UV-Vis spectrophotometer. The properties of AgNPs were assessed using Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD). Also, their antibacterial effects were checked against Staphylococcus aureus and Escherichia coli. Results:: It was revealed that 5-50 nm sized spherical to elongated nanoparticles were synthesized that possessed comparatively better antibacterial potential against E. coli and S. aureus than conventional extract of the E. japonica leaves. Conclusions:: Green synthesis and effective utilization of Eriobotrya japonica extract loaded silver nanoparticles is a promising approach for nanoparticle production avoiding negative environmental impacts.

scholarly journals Electrospun Silver Coated Polyacrylonitrile Membranes for Water Filtration Applications

Membranes ◽  
2018 ◽  
Vol 8 (3) ◽  
pp. 59 ◽  
Author(s):  
Shalv Parekh ◽  
Rebecca David ◽  
Kranthi Bannuru ◽  
Lakshminarasimhan Krishnaswamy ◽  
Avinash Baji
Keyword(s):  
Silver Nanoparticles ◽  
Low Cost ◽  
Silver Ions ◽  
Antimicrobial Effect ◽  
Water Filtration ◽  
Porous Membranes ◽  
Oh Groups ◽  
Underdeveloped Countries ◽  
Latex Beads ◽  
Particulate Filtration

The scarcity of drinking water and the contamination of water sources in underdeveloped countries are serious problems that require immediate low-tech and low-cost solutions. In this study, we fabricated polyacrylonitrile (PAN) porous membranes coated with silver nanoparticles (AgNP) and demonstrated their use for water filtration and water treatment applications. The membranes were prepared by electrospinning a PAN solution and treating in a hydroxylamine (NH2OH) aqueous solution to form –C(NH2)N–OH groups that were used for functionalization (Ag+ ions) of the membrane. The coordinated silver ions were then converted to silver nanoparticles. The microstructure of the membrane, water permeability, antimicrobial effect (using Escherichia coli), and particulate filtration capabilities were studied. This study verified that the membrane demonstrated a 100% reduction for Gram-negative bacteria with an effective filtration rate of 8.0 mL/cm2 min. Furthermore, the membrane was able to eliminate 60% of latex beads as small as 50 nm and over 80% of the 2 µm beads via gravity filtration. This study demonstrated that PAN–AgNP membranes can be employed as antimicrobial membranes for the filtration of water in underdeveloped countries.

Effect of silver nanoparticles on biofilms of microorganisms (review)

10.12737/8117 ◽  
2015 ◽  
Vol 9 (1) ◽  
pp. 0-0
Author(s):  
Гладких ◽  
P. Gladkikh
Keyword(s):  
Silver Nanoparticles ◽  
High Efficiency ◽  
Antibacterial Properties ◽  
Silver Ions ◽  
Experimental Models ◽  
Human Pathogens ◽  
Antibiotic Resistant ◽  
Medical Supplies ◽  
Antibiotic Drugs

Nanotechnology enter in all spheres of human activity, particularly in medicine. Using nanoprogress allows the use of various nanostructures in the traditional methods therapy of various pathologies. The growing resistance to antibiotic drugs among human pathogens strains of microorganisms requires the development of alternatives. Silver nanoparticles are current contenders for the role of an alternative to modern antibiotics. They are able to transfer therapeutic agents, act on the membrane of microbial cells, disrupting their livelihoods and leading to death, as they are less toxic than silver ions. The use of nanoparticles is an effective tool in combating formed biofilms on medical supplies, and prevent to their education. Nanoparticles have proved positively against many antibiotic-resistant strains of microorganisms, including planktonic phase P. aureginosa. Synergistic effect observed while using of nanoparticles and various antibiotics. Antibacterial properties studied in experimental models of peritonitis and meningoencephalitis. In case of peritonitis high efficiency.

scholarly journals Effects of Polydopamine Microspheres Loaded with Silver Nanoparticles on Lolium multiflorum: Bigger Size, Less Toxic

Toxics ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 151
Author(s):  
Xinrui Wang ◽  
Hongyong Luo ◽  
Weihua Zheng ◽  
Xinling Wang ◽  
Haijun Xiao ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Shoot Growth ◽  
Antibacterial Property ◽  
Lolium Multiflorum ◽  
Rapid Development ◽  
Antibacterial Properties ◽  
Silver Ions ◽  
Green Method ◽  
Adverse Impacts ◽  
Root And Shoot Growth

The rapid development of nanotechnology and its widespread use have given rise to serious concerns over the potential adverse impacts of nanomaterials on the Earth’s ecosystems. Among all the nanomaterials, silver nanoparticles (AgNPs) are one of the most extensively used nanomaterials due to their excellent antibacterial property. However, the toxic mechanism of AgNPs in nature is still unclear. One of the questions under debate is whether the toxicity is associated with the size of AgNPs or the silver ions released from AgNPs. In our previous study, a sub-micron hybrid sphere system with polydopamine-stabilized AgNPs (Ag@PDS) was synthesized through a facile and green method, exhibiting superior antibacterial properties. The current study aims to explore the unique toxicity profile of this hybrid sphere system by studying its effect on germination and early growth of Lolium multiflorum, with AgNO3 and 15 nm AgNPs as a comparison. The results showed the seed germination was insensitive/less sensitive to all three reagents; however, vegetative growth was more sensitive. Specifically, when the Ag concentration was lower than 40 mg/L, Ag@PDS almost had no adverse effects on the root and shoot growth of Lolium multiflorum seeds. By contrast, when treated with AgNO3 at a lower Ag concentration of 5 mg/L, the plant growth was inhibited significantly, and was reduced more in the case of AgNP treatment at the same Ag concentration. As the exposures of Ag@PDS, AgNO3, and AgNPs increased, so did the Ag content in the root and shoot. In general, Ag@PDS was proven to be a potential useful hybrid material that retains antibacterial property with light phytotoxicity.

scholarly journals Effect of Exchangeable Ions in Natural and Modified Zeolites on Ag Content, Ag Nanoparticle Formation and Their Antibacterial Activity

Materials ◽  
2021 ◽  
Vol 14 (15) ◽  
pp. 4153
Author(s):  
Neli Mintcheva ◽  
Marinela Panayotova ◽  
Gospodinka Gicheva ◽  
Orlin Gemishev ◽  
Georgy Tyuliev
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Agents ◽  
Diffuse Reflectance ◽  
Antibacterial Properties ◽  
Silver Ions ◽  
Modified Zeolite ◽  
End Of Treatment ◽  
Modified Zeolites ◽  
Size And Morphology ◽  
Microscopic Techniques

To broaden the application of silver nanoparticles (AgNPs), which are well-known antibacterial agents, they are supported on different substrates to prevent aggregation, increase their surface area and antibacterial efficiency, and to be separated from the system more effectively at the end of treatment. To produce nanocomposites that consist of silver nanoparticles on natural and modified zeolites, silver ions (Ag+) were loaded onto zeolite (natural, Na-modified, H-modified) and then thermally reduced to AgNPs. The effect of the exchangeable cations in zeolite on Ag+ uptake, AgNPs formation, size and morphology was investigated by the TEM, SEM, EDX, XPS, UV-vis, XRD and BET methods. The silver amount in the nanocomposites decreased in the following order Na-modified zeolite > natural zeolite > H-modified zeolite. Microscopic techniques showed formation of AgNPs of 1–14 nm on natural and Na-modified zeolite, while the diameter of metal particles on H-modified zeolite was 12–42 nm. Diffuse reflectance UV-vis and XPS methods revealed the presence of both silver ions and AgNPs in the materials indicating that partial reduction of Ag+ ions took place upon heating at 400 °C in air. Additionally, antibacterial properties of the nanocomposites were tested against Escherichia coli, and it was found that Ag–containing composites originating from the Na-modified zeolite demonstrated the highest activity.

scholarly journals Antibacterial Effect of Colloidal Suspensions Varying in Silver Nanoparticles and Ions Concentrations

Nanomaterials ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 31
Author(s):  
Varvara Platania ◽  
Alexandra Kaldeli-Kerou ◽  
Theodora Karamanidou ◽  
Maria Kouki ◽  
Alexander Tsouknidas ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Effect ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Colloidal Suspensions ◽  
Silver Ions ◽  
Cellular Level ◽  
Bacterial Strains ◽  
Three Samples ◽  
Flow Filtration

A lot of effort has been dedicated recently to provide a better insight into the mechanism of the antibacterial activity of silver nanoparticles (AgNPs) colloidal suspensions and their released silver ionic counterparts. However, there is no consistency regarding whether the antibacterial effect displayed at cellular level originates from the AgNPs or their ionic constitutes. To address this issue, three colloidal suspensions exhibiting different ratios of AgNPs/silver ions were synthesized by a wet chemistry method in conjunction with tangential flow filtration, and were characterized and evaluated for their antimicrobial properties against two gram-negative, Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa), and two gram-positive, Staphylococcus aureus (S. aureus) and Staphylococcus epidermidis (S. epidermidis), bacterial strains. The produced samples contained 25% AgNPs and 75% Ag ions (AgNP_25), 50% AgNPs and 50% Ag ions (AgNP_50), and 100% AgNPs (AgNP_100). The sample AgNP_100 demonstrated the lowest minimum inhibitory concentration values ranging from 4.6 to 15.6 ppm for all four bacterial strains, while all three samples indicated minimum bactericidal concentration (MBC) values ranging from 16.6 ppm to 62.5 ppm against all strains. An increase in silver ions content results in higher bactericidal activity. All three samples were found to lead to a significant morphological damage by disruption of the bacterial cell membranes as analyzed by means of scanning electron microscopy (SEM). The growth kinetics demonstrated that all three samples were able to reduce the bacterial population at a concentration of 3.1 ppm. SEM and growth kinetic data underline that S. epidermidis is the most sensitive among all strains against the investigated samples. Our results showed that all three AgNPs colloidal suspensions exhibited strong antibacterial properties and, thus, they can be applied in medical devices and antimicrobial control systems.

Sign in / Sign up

Export Citation Format

Share Document