Characteristic of Biomaterial from Fermentation Product and its Effectto Enzyme Activity of Clarias sp.

2021 ◽  
Vol 1028 ◽  
pp. 333-338
Author(s):  
Yuli Andriani ◽  
Walim Lili ◽  
Irfan Zidni ◽  
Risdiana
Keyword(s):  
Enzyme Activity ◽  
Digestive Tract ◽  
Stability Test ◽  
Durability Test ◽  
Physical Quality ◽  
Sinking Rate ◽  
Average Value ◽  
Protease Enzyme ◽  
Breaking Time ◽  
Fermented Products

The physical characteristics of fermented products from restaurant waste food with BIOMS probiotic at a dose of 8% which was incubated for 7 days were investigated in order to study effect of fermentation to the physical characteristic of biomaterial and its effect to enzyme activity of Clarias sp. Fermented products are added to the feed with doses of 0, 10%, 20%, 30% and 40% and physical tests include breaking time, sinking rate test, durability test, and stability test. Furthermore, the feed was given to catfish for 40 days and the enzyme activity (amylase and protease) was analyzed in its digestive tract at the end of the study. It is showed that the addition of fermented products decreased the physical quality of the feed. The best physical quality was found in the control feed with an average value of breaking time of 22.98 hours, an average value of sinking rate of 0.53 cm/s, a durability test of 97.23 %, and a stability test value of 93.53% (10 minutes), 88.8% (30 minutes), and 76.26% (60 minutes). For feed products with doses of fermented products of 10%, 20%, 30%, and 40%, the values ​​of breaking time and sinking rate, durability and stability were lower compared to that of control feed products. Meanwhile, the enzyme activity in the digestive tract of catfish shows that giving a dose of 20% fermented products in the feed resulted in the best composition of amylase and protease enzyme activity of 9.021 units/mL and 275 units/mL. These results indicated that improvements are needed in the fermentation process to produce better physical quality and suitable for catfish feed.

scholarly journals Distribusi Bakteri Penghasil Enzim Ekstraseluler Pada Saluran Pencernaan Lobster Mutiara (Panulirus ornatus)

2019 ◽  
Vol 5 (2) ◽  
pp. 71
Author(s):  
Fatur Rahman ◽  
Ismiati Ismiati ◽  
Arbai Nurhasanah
Keyword(s):  
Enzyme Activity ◽  
Digestive Tract ◽  
Extracellular Enzymes ◽  
Extracellular Enzyme ◽  
Extracellular Enzyme Activity ◽  
Bacterial Isolates ◽  
Clear Zone ◽  
Zone Diameter ◽  
Protease Enzyme ◽  
Proteolytic Bacteria

The activity of the digestive function of animals is influenced by the secretion of extracellular enzymes from bacteria in the digestive tract. This study aims to evaluate the distribution of bacteria producing protease enzyme, amylase and lipase from the digestive tract of pearl lobster, Panulirus ornatus. Bacterial isolates that have extracellular enzyme activity are based on their ability to form clear zones in the test media. The results showed that of 51 bacterial isolates from the digestive tract of P. ornatus, proteolytic bacteria were 27.45%, amylolytic bacteria were 23.53% and lipolytic bacteria were 21.77%. Based on bacterial dominance in the gastrointestinal segment, namely the cardiac, piloric and intestinal sections, it was dominated by amylolytic bacteria at 33.33%, proteolytic at 37.50% and lipolytic at 29.41%. The activity of proteolytic, amylolytic and lipolytic bacteria based on the highest clear zone diameter was achieved respectively by SP5 isolates of 12 mm, SK10 isolates of 21 mm and SU15 isolates of 20 mm. The three bacterial isolates were potential as probiotic aquacultur candidates

Effect of Adding Fermented Restaurant Waste Meal with Different Concentration to Physical Quality of Fish Pellet

2020 ◽  
pp. 1-7 ◽  
Author(s):  
Meri Alex Sandra ◽  
Yuli Andriani ◽  
Kiki Haetami ◽  
Walim Lili ◽  
Irfan Zidni ◽  
...  
Keyword(s):  
Stability Control ◽  
Control Fish ◽  
Fish Feed ◽  
Durability Test ◽  
Physical Quality ◽  
Sinking Rate ◽  
Significant Difference ◽  
Rate Test ◽  
Restaurant Waste

This study aims to evaluate the physical quality of fish feed pellets formulated with different concentration of fermented restaurant waste meal (FRWM). The treatments given consisted of treatment A (control, without adding FRWM), treatment B (adding FRWM 10%), treatment C (adding FRWM 20%), treatment D (adding FRWM 30%) and treatment E (adding FRWM 40%). The study was conducted from July to October 2019 at the Aquaculture Laboratory and Hatchery in Ciparanje of the Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran. The parameters observed include the breaking rate test, sinking rate test, durability test, and stability test. Results showed that the use of FRWM given significant difference (p<0.05) for breaking rate and stability value. However, result showed that the sinking rate and durability value generally did not show significant difference (p>0.05). The FRWM can be used up to a concentration of 30% which gives effect to the sinking rate of the fish pellet which is similar to the control fish pellet namely 0.38 cm/s while the concentration of the meal of the FRWM gives the closest value of breaking rate and stability control as much as treatment B (adding FRWM 10%) with a break rate for 19.93 hours and stability in water for 10 minutes by 91.66%, 30 minutes by 87.60%, and for 60 minutes by 75.26%. The different adding concerntration of FRWM gives effect to the quality of fish pellet while more than 10% can decrease its quality. The difference were show in all treatment in some parameters observed.

Digestive enzyme and gut surfactant activity of detritivorous gizzard shad (Dorosoma cepedianum)

2000 ◽  
Vol 57 (6) ◽  
pp. 1113-1119 ◽  
Author(s):  
James C Smoot ◽  
Robert H Findlay
Keyword(s):  
Enzyme Activity ◽  
Protease Activity ◽  
Feeding Strategy ◽  
Digestive Enzyme ◽  
Artificial Substrates ◽  
Gizzard Shad ◽  
Dorosoma Cepedianum ◽  
Digestive Physiology ◽  
Protease Enzyme ◽  
Surfactant Activity

Measuring digestive enzyme and surfactant activities tested specialization of gizzard shad (Dorosoma cepedianum) digestive physiology to a detritivorous feeding strategy. Digestive enzyme activity was measured in adult and larval gizzard shad using fluorescently labeled artificial substrates. Surfactant activity in gizzard shad was measured by comparing gut juice drop diameters over a range of dilutions. Enzyme activity in the ceca region of adult gizzard shad was high for esterase, beta-glucosidase, lipase, and protease. Enzyme activity was lower in posterior intestine sections than in anterior intestine sections, although protease activity remained high for the greatest distance in the intestine. Micelles were detected in adult gizzard shad gut juice, and surfactant activity was greatest in the ceca region. Larval gizzard shad protease activity was similar to that of adult fish, and surfactants were below their critical micelle concentration. Gizzard shad coupled digestive physiology with gut anatomy to obtain nutrients from detritus, and these adaptations may explain elevated growth rates observed in these fish when they are planktivorous.

Separation and characterization of venom components in Loxosceles reclusa—II. Protease enzyme activity

Toxicon ◽  
1979 ◽  
Vol 17 (6) ◽  
pp. 529-537 ◽  
Author(s):  
Yaw-Shong Jong ◽  
B.R. Norment ◽  
James R. Heitz
Keyword(s):  
Enzyme Activity ◽  
Protease Enzyme ◽  
Loxosceles Reclusa

scholarly journals Desensitization of Capsaicin-Sensitive Afferents Accelerates Early Tumor Growth via Increased Vascular Leakage in a Murine Model of Triple Negative Breast Cancer

2021 ◽  
Vol 11 ◽  
Author(s):  
Noémi Bencze ◽  
Csaba Schvarcz ◽  
Gábor Kriszta ◽  
Lea Danics ◽  
Éva Szőke ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Enzyme Activity ◽  
Tumor Growth ◽  
Triple Negative ◽  
Vascular Leakage ◽  
Size Difference ◽  
Control Group ◽  
Protease Enzyme

There is growing interest in the role of nerve-driven mechanisms in tumorigenesis and tumor growth. Capsaicin-sensitive afferents have been previously shown to possess antitumoral and immune-regulatory properties, the mechanism of which is currently poorly understood. In this study, we have assessed the role of these terminals in the triple negative 4T1 orthotopic mouse model of breast cancer. The ultrapotent capsaicin-analogue resiniferatoxin (RTX) was used for the selective, systemic desensitization of capsaicin-sensitive afferents. Growth and viability of orthotopically implanted 4T1 tumors were measured by caliper, in vivo MRI, and bioluminescence imaging, while tumor vascularity and protease enzyme activity were assessed using fluorescent in vivo imaging. The levels of the neuropeptides Calcitonin Gene-Related Peptide (CGRP), Substance P (SP), and somatostatin were measured from tumor tissue homogenates using radioimmunoassay, while tumor structure and peritumoral inflammation were evaluated by conventional use of CD31, CD45 and CD3 immunohistology. RTX-pretreated mice demonstrated facilitated tumor growth in the early phase measured using a caliper, which was coupled with increased tumor vascular leakage demonstrated using fluorescent vascular imaging. The tumor size difference dissipated by day seven. The MRI tumor volume was similar, while the intratumoral protease enzyme activity measured by fluorescence imaging was also comparable in RTX-pretreated and non-pretreated animals. Tumor viability or immunohistopathological profile was measured using CD3, CD31, and CD45 stains and did not differ significantly from the non-pretreated control group. Intratumoral somatostatin, CGRP, and SP levels were similar in both groups. Our results underscore the beneficial, antitumoral properties of capsaicin sensitive nerve terminals in this aggressive model of breast cancer, which is presumed to be due to the inhibition of tumor vascular bed disruption. The absence of any difference in intratumoral neuropeptide levels indicates non-neural sources playing a substantial part in their expression.

scholarly journals Karakteristik Sifat Mekanik Wadah Makanan dari Limbah Daun Pisang Kering

2021 ◽  
Vol 6 (1) ◽  
pp. 27-34
Author(s):  
Zilmi Azyurah Rahman ◽  
Hernawati Hernawati ◽  
Ayusari Wahyuni
Keyword(s):  
Water Absorption ◽  
Absorption Test ◽  
Steel Mold ◽  
Physical Quality ◽  
Large Plate ◽  
Average Value ◽  
Water Absorption Test ◽  
Quality Of Food ◽  
Food Media

The objective of this research is to determine the physical quality of food media, consisting of small plate, large plate and small bowl. The method used of this research is testing the water absorption and resistance of the food media’s temperature. The process of making a food media using a stainless steel mold which is heated using a food thermometer with 100   and a load of 10 kg as a pressure, then it is printed for 5 minutes with 4 times taking the data for each food media. There are two testing of food media, the first test is water absorption with 29.5  for 125 miles into a small plate, 200 miles into a small bowl and 250 miles into a large plate for 60 minutes, the average value of the water absorption test for the Non-woven food media consists of three forms food medias have 0.00  as the same result, meanwhile woven media such as small plate has 65.00 , large plate has 44.83 and small bowl has 66.68 .  Second test is resistance of the food medias based on temperature parameters. The temperatures used were 40, 60, 80 for non-woven food media consists of three forms food media which had few change after drying, while for woven food media consists three forms of food media had the same results, that is seepage (leaking) in the food media.

scholarly journals Screening Cellulolytic Bacteria from the Digestive Tract Snail (Achatina fulica) and Test the Ability of Cellulase Activity

2016 ◽  
Vol 8 (3) ◽  
pp. 386
Author(s):  
Wijanarka Wijanarka ◽  
Endang Kusdiyantini ◽  
Sarjana Parman
Keyword(s):  
Enzyme Activity ◽  
Digestive Tract ◽  
Generation Time ◽  
Cellulase Activity ◽  
Biology Education ◽  
Activity Measurement ◽  
Bacterial Isolates ◽  
Achatina Fulica ◽  
Cellulolytic Bacteria ◽  
Activity Measurements

<p>On the research of enzyme production levels observed cellulase produced by bacteria in the digestive tract of the isolation of the Snail (<em>Achatina fulica</em>). Isolation of bacteria based on the ability of bacteria to grow on CMC media. The purpose of this study was to determine cellulase activity by cellulolytic bacteria. Some bacterial isolates were identified as cellulolytic bacteria, they were KE-B1, KE-B2, KE-B3, KE-B4, KE-B5, and KE-B6. Isolates KE-B6 was the best isolates. Furthermore KE-B6 isolates were grown on media production to determine the pattern of growth and enzyme activity. Measurement of cell growth was conducted by inoculating starter aged 22 hours at CMC production of liquid medium. Cellulase enzyme activity measurements was performed by the DNS method. The results showed that the highest activity by new isolate bacteria KE-B6 and its value of the activity of 0.4539 U/mL, growth rate (µ) 0.377/hour and generation time (g) 1.84 hour. This research expected cellulase of producing bacteria were easy, inexpensive and efficient. This enzyme can be used as an enzyme biolytic once expected to replace expensive commercial enzyme. The biotylic enzyme can be applied to strains improvement (protoplast fusion).</p><p><strong>How to Cite</strong></p><p>Wijanarka, W., Kusdiyantini, E. &amp; Parman, S. (2016). Screening Cellulolytic Bacteria from the Digestive Tract Snail (<em>Achatina fulica</em>) and Test the Ability of Cellulase Activity. <em>Biosaintifika: Journal of Biology &amp; Biology Education</em>, 8(3), 386-392. </p>

scholarly journals Influence of enzymes and extraction conditions on high yield of cottonseed milk

2021 ◽  
Vol 42 (4(SI)) ◽  
pp. 1195-1200
Author(s):  
S. Thirukkumar ◽  
◽  
G. Hemalatha ◽  
S. Vellaikumar ◽  
M. Murugan ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Protein Content ◽  
Milk Yield ◽  
Hydrolytic Enzymes ◽  
Control Sample ◽  
High Yield ◽  
Chemical Characteristics ◽  
Protease Enzyme ◽  
Physico Chemical ◽  
Incubation Temperatures

Aim: This research aimed to optimize suitable hydrolytic enzymes for maximizing cottonseed milk extracts for high cottonseed milk yield, protein content and low gossypol level. Methodology: Known amount of cottonseed was soaked for 90 min at 32°C and blended (cottonseed:water@1:6). Different aliquots of the blended cottonseed slurry were treated with 1% of enzymes viz., protease, cellulase and α-amylase enzyme at pH 7.0 followed by incubation at 40 and 52°C for 2.30 hr for the extraction of cottonseed milk. The enzyme activity of extracted milk was subsequently inactivated by pasteurization (90°C, 5 min). Further analysis of physico-chemical characteristics was also carried. The control sample included milk extraction from non-enzyme treated cottonseed milk extract (30±2°C). Results: Among different treatments, cottonseed milk extraction using protease enzyme at 40°C incubation showed the highest milk yield (86.71%) with the lowest sedimentation (3.72%). Further incubation 40°C and 52°C showed the highest protein content of 2.10 and 2.27 g 100 ml-1 and gossypol reduction of 40.36 and 35.22%, respectively, in the cottonseed milk extract. Meanwhile, cellulase and α-amylase enzymes treated samples at both incubation temperatures showed poor physico-chemical characteristics as compared to control. Interpretation: Protease enzyme seems to be the most suitable for optimum or higher extraction of cottonseed milk.

scholarly journals Production of Garbage Enzyme from Different Fruit and Vegetable Wastes and Evaluation of its Enzymatic and Antimicrobial Efficacy

2019 ◽  
Vol 6 ◽  
pp. 113-118 ◽  
Author(s):  
Karuna Neupane ◽  
Rama Khadka
Keyword(s):  
Antimicrobial Activity ◽  
Enzyme Activity ◽  
Carica Papaya ◽  
Salmonella Typhi ◽  
Ananas Comosus ◽  
Antimicrobial Efficacy ◽  
Brown Sugar ◽  
E Coli ◽  
Protease Enzyme ◽  
Vegetable Wastes

Objectives: To evaluate the enzymatic and antimicrobial efficacy of enzyme from garbage produced from different fruits and vegetable wastes. Methods: This study was conducted from October-2018 to February-2019 in the laboratory of Padma Kanya Multiple College, Bagbazar, Kathmandu, Nepal. This study was carried for production, analysis of enzymatic and antimicrobial efficacy by using yeast (Saccharomyces cerevisae) and bacteria (Bacillus species) in 5 fruits peels, Mosambi (Citrus limetta), Pomegranate (Punica granatum), Pineapple (Ananas comosus), Papaya (Carica papaya) and mixed fruits collected from fresh fruit stall and vegetable peels collected from college’s hostel. The fermentation mixture was made in the ratio 1:3:10 (1 part brown sugar, 3 parts fruits/vegetable peels and 10 parts water) and left for 3 months for fermentation. Results: After fermentation, enzyme activity (amylase, protease, caseinase, cellulase and lipase) and antimicrobial efficacy (S. aureus, S. aureus (ATCC 25923), Bacillus spp, Salmonella Typhi, E. coli, E. coli (ATCC 25922), Shigella spp, Pseudomonas aeruginosa) were analyzed. All the samples showed amylase and caseinase enzyme activity, only Pineapple (Ananascomosus), Papaya (Carica papaya) and Mixed fruit showed protease enzyme activity while only Pomegranate (Punicagranatum) showed lipase enzyme activity. In antimicrobial efficacy test, garbage enzyme produced from vegetable sample didn’t show antimicrobial activity with bacteria used except E. coli (ATCC 25922)and S. aureus (ATCC 25923). Similarly, garbage enzyme produced from Mixed fruit and Papaya (Carica papaya) didn’t show antimicrobial activity with Salmonella Typhi and S. aureus (ATCC 25923) respectively but garbage enzyme from other wastes showed antimicrobial activity with bacteria used in test. Conclusion: Different fruits and vegetables wastes showed different enzyme activity and antimicrobial activity.

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