scholarly journals Influence of enzymes and extraction conditions on high yield of cottonseed milk

2021 ◽  
Vol 42 (4(SI)) ◽  
pp. 1195-1200
Author(s):  
S. Thirukkumar ◽  
◽  
G. Hemalatha ◽  
S. Vellaikumar ◽  
M. Murugan ◽  
...  

Aim: This research aimed to optimize suitable hydrolytic enzymes for maximizing cottonseed milk extracts for high cottonseed milk yield, protein content and low gossypol level. Methodology: Known amount of cottonseed was soaked for 90 min at 32°C and blended (cottonseed:water@1:6). Different aliquots of the blended cottonseed slurry were treated with 1% of enzymes viz., protease, cellulase and α-amylase enzyme at pH 7.0 followed by incubation at 40 and 52°C for 2.30 hr for the extraction of cottonseed milk. The enzyme activity of extracted milk was subsequently inactivated by pasteurization (90°C, 5 min). Further analysis of physico-chemical characteristics was also carried. The control sample included milk extraction from non-enzyme treated cottonseed milk extract (30±2°C). Results: Among different treatments, cottonseed milk extraction using protease enzyme at 40°C incubation showed the highest milk yield (86.71%) with the lowest sedimentation (3.72%). Further incubation 40°C and 52°C showed the highest protein content of 2.10 and 2.27 g 100 ml-1 and gossypol reduction of 40.36 and 35.22%, respectively, in the cottonseed milk extract. Meanwhile, cellulase and α-amylase enzymes treated samples at both incubation temperatures showed poor physico-chemical characteristics as compared to control. Interpretation: Protease enzyme seems to be the most suitable for optimum or higher extraction of cottonseed milk.

Author(s):  
Р.Х. КАНДРОКОВ ◽  
С.Е. ТЕРЕНТЬЕВ ◽  
Н.В. ЛАБУТИНА ◽  
М.Ш. БЕГЕУЛОВ ◽  
И.Г. БЕЛЯВСКАЯ ◽  
...  

В России наблюдается повышенный интерес к продуктам питания, включающим нетрадиционное сырье. Спрос на продукты с добавкой семян конопли (Cannabis sativa L.) обусловлен их питательной ценностью и низкой аллергенностью. Исследованы химические и физико-химические показатели пшенично-конопляной муки, полученной из помольной смеси зерна яровой пшеницы сорта Радмира и семян конопли сорта Сурская в соотношении соответственно 95 : 5, 92,5 : 7,5 и 90 : 10%. В качестве контрольного образца была пшеничная мука без добавок. Размол помольных пшенично-конопляных смесей различного соотношения и зерна пшеницы проводили на мельницах лабораторного помола (МЛП-4) с нарезными (драные системы) и микрошероховатыми вальцами (размольные системы). Исследование химических и физико-химических свойств образцов пшенично-конопляной и пшеничной муки проводили на инфракрасном анализаторе SpectraStar 2500 XL. Установлено, что добавление семян конопли в помольную смесь существенно снижает выход пшенично-конопляной муки по сравнению с выходом пшеничной муки (при добавке 5–7,5% семян конопли выход снижается на 4,3–4,4%, а при добавке 10% семян конопли – на 10,6%), однако повышается содержание жира и белка во всех потоках пшенично-конопляной муки, полученной как с драных, так и с размольных систем. По сравнению с содержанием жира и белка в муке из зерна пшеницы, составившим 1,12 и 11,57% соответственно, при добавлении 5% семян конопли в помольную пшенично-конопляную зерновую смесь средневзвешенное содержание жира в пшенично-конопляной муке составило 3,76%, а содержание белка – 12,74%, при добавлении 7,5% конопли в помольную смесь средневзвешенное содержание жира в пшенично-конопляной муке составило 4,35%, а белка – 12,7%, при добавлении 10% конопли в помольную смесь средневзвешенное содержание жира в пшенично-конопляной муке составило 4,97%, а содержание белка – 12,4%. Повышение содержания жира и белка в пшенично-конопляной муке будет способствовать повышению пищевой ценности хлебобулочного изделия из нее. Increased interest in food products, including non-traditional raw materials, is observe among the population of Russia. The demand for products with the addition of hemp seeds (Cannabis sativa L.) is due to their nutritional value and low allergenicity. Chemical and physico-chemical parameters of wheat-hemp flour obtained from a grind mixture of Radmira spring wheat grain and Surskaya hemp seeds in the ratio: 95 : 5, 92,5 : 7,5 and 90 : 10%, respectively, were investigated. Wheat flour without additives was use as a control sample. Grinding of grind wheat-hemp mixtures of various ratios and wheat grains was carried out in laboratory grind mills with rifled (torn systems) and micro-roughened rollers (grinding systems). The study of chemical and physico-chemical properties of wheat-hemp and wheat flour samples was carried out on the SpectraStar 2500 XL infrared analyzer. It was found that the addition of hemp seeds to the grind mixture significantly reduces the yield of wheat-hemp flour compared with the yield of wheat flour (with the addition of 5–7,5% of hemp seeds, the yield decreases by 4,3–4.4%, and with the addition of 10% of hemp seeds – by 10,6%), however increases the fat and protein content in all streams of wheat-hemp flour obtained from both torn and grinding systems. Compared with the fat and protein content in wheat flour, which amounted to 1,12 and 11,57% respectively, when adding 5% hemp to the wheat-hemp grain mixture, the weighted average fat content in wheat-hemp flour was 3,76%, and the protein content was 12,74%, when adding 7,5% hemp to the grind mixture, the weighted average fat content in wheat-hemp flour was 4,35%, and protein – 12,7%, and when adding 10% the weighted average fat content in wheat-hemp flour in the grind mixture was 4,97%, and the protein content was 12,4%. An increase in the fat and protein content in wheat and hemp flour will contribute to an increase in the nutritional value of a bakery product made from it.


1975 ◽  
Vol 38 (7) ◽  
pp. 401-405
Author(s):  
M. W. PULLE ◽  
K. INO

Physico-chemical parameters that influence dough attributes and baking quality were investigated in single and composite flours. Considerable variation in the starch and protein components was observed in the different formulations of wheat and substituent flours. Incorporation of kurakkan, sorghum, or manioc flour increased gelatinization temperature, viscosity, and amylose content irrespective of the extent of substitution. Although increases in amylose content were within tolerable limits, changes in gelatinization temperature and viscosity characteristics necessitate use of chemical agents and modified technology for preparation of acceptable products. A marked reduction in protein content was revealed in manioc-wheat composites, wherein nutritive fortification should comprise an additional requisite.


Author(s):  
Janki Suthar ◽  
A.H. Jana ◽  
Hiral Modha ◽  
Smitha Balakrishnan

The research was carried out to ascertain the feasibility of preparing paneer from milk blend containing low pressure homogenized milk to avail the benefits rendered by homogenization. Standardized milk was subjected to two-stage homogenization (4.90 and 0.98 MPa respectively) and then blended with unhomogenized standardized milk in three proportions (i.e. 3:7, 4:6 and 1:1, w/w). It was necessary to add calcium chloride to the milk blend to improve the firmness of resultant paneer. The experimental paneer samples obtained from ‘milk blend’ containing homogenized milk, as well as control sample (only from unhomogenized milk) were studied for their proximate composition, physico-chemical characteristics, textural properties and sensory quality. Paneer obtained from milk blend (homogenized:unhomogenized; 4:6 w/w) resulted in greater fat recovery and moisture content culminating in significantly higher yield compared to control paneer (CP). The hardness of paneer obtained from blended milks was lower, but the springiness of BMP3:7 sample was greater than that of CP. The total sensory score of experimental paneer BMP4:6 was similar to the score associated with CP, but greater than the scores associated with paneer samples BMP3:7 and BMP1:1. Blending of homogenized (low pressure) milk with unhomogenized milk in 4:6 proportion helped in obtaining paneer with superior fat recovery and yield compared to use of unhomogenized milk, without any adverse effect on sensory properties and with concomitant cost savings.


2020 ◽  
Vol 09 ◽  
Author(s):  
Shirin Saberianpour ◽  
Leila Abkhooie ◽  
Babak Elyasifar ◽  
Azita Dilmaghani

Background : Proteases are the most important industrial enzymes with diverse applications in. Bacteria, such as Bacillus, commonly used to produce protease for industrial purposes. Proteases are commercially exploited in large-scale, especially in pharmaceutical, food, leather and detergent industries. Objective: The aim of this study was screening and optimization of protease enzyme activity produced by local bacteria. Method: In this research, the effect of incubation time, temperature and initial pH were investigated to improve the extracellular protease enzyme activity by two bacteria, named Bacillus subtilis strain DAR and Alkalihalobacillus hwajinpoensis strain 3NB. These two isolates have already been isolated and registered from Iran. Results: The results indicated that the optimum incubation time for protease activity in B. subtilis strain DAR is 36 h in contrast to 40 h in Alkalihalobacillus hwajinpoensis strain 3NB. The optimum incubation temperatures for enzyme activity for B. subtilis and Alkalihalobacillus hwajinpoensis is 50°C and 40°C, respectively. Optimum pH conditions for protease activity for both of the bacteria is 8. Conclusion: In current study, we investigated the optimum incubation time, pH and temperature for best protease activity. Further studies are recommended to improve protease activity through changing enzyme substrates.


LWT ◽  
2011 ◽  
Vol 44 (8) ◽  
pp. 1827-1834 ◽  
Author(s):  
Fatma Abbès ◽  
Mohamed Ali Bouaziz ◽  
Christophe Blecker ◽  
Manel Masmoudi ◽  
Hamadi Attia ◽  
...  

2020 ◽  
Vol 9 (1) ◽  
pp. 1047
Author(s):  
Jayadi Jayadi ◽  
Amrah Husma ◽  
Nursyahran Nursyahran

Marosatherina ladigesi’s original habitat is in the freshwater of South Sulawesi as endemic fish in the wallacea zone Indonesia, with a local name called beseng-beseng fish. M.ladigesi was already in the category of at threat of extinction.The purpose of this study was to investigate the effect of different protein levels on survival rate, growth performance, body protein composition and enzyme activity of protease, lipase and amylase of beseng-beseng fish. The research method was using Completely Randomized Design with three treatments and three replications. Treatment of the study: protein content of feed A: 40%, B: 50% and C: 60%. Fish maintenance takes 90 days. The results showed that protein content had a significant effect (P<0,05) on absolute body growth, specific growt rate, survival rate, body protein composition and protease enzyme activity of juvenile beseng-beseng fish. The best growth performance is obtained at 50% and 60% protein content, while the survival and body protein composition and the best protease enzyme activity at 60% protein content.


1971 ◽  
Vol 103 (3) ◽  
pp. 454-457 ◽  
Author(s):  
Hans Laufer ◽  
Ki Ssu Schin

AbstractChanges in the hydrolytic enzymes, ribonuclease and acid phosphatase were investigated in the salivary gland of Chironomus tentans since these lysosomal enzymes may participate in hormonally stimulated tissue breakdown. Quantitative assays revealed 9- and 12-fold increases in the specific activity of these enzymes during pupation while the protein content of the gland was decreasing. These increases cannot be accounted for by decreased protein but may represent an activation, accumulation, or synthesis which seems to be important in gland breakdown at metamorphosis.


2019 ◽  
Vol 26 (01) ◽  
pp. 65-73
Author(s):  
Sandagdorj B ◽  
Baigalmaa Ts ◽  
Purevsuren D ◽  
Burenjargal S ◽  
Gombojav A ◽  
...  

To deepen understandings on changes in mare milk yield and composition along with lactation,  physico-chemical characteristics and freshness of raw mare milk this study was conducted. The first  trial on the change in mare milk yield and composition was conducted along with different lactation  period using 7 adult mares. Milk yield was determined volumetrically after 2 hour foal separation.  Hand milking with milk ejection by foal was applied. Milk yield reached at peak in August which  corresponds to around 90th days of lactation. Lactose content reaches its max value at 90th day of  lactation also and it remains as high as its peak until the milking season ends while the yield was  decreased significantly (p≤0.05). Fat and protein contents were gradually decreased to the end of  milking season. To conduct quality evaluation of raw mare milk it is vitally important to know  physico-chemical characteristics. To clarify did this second trial in comparison with cow milk.  Results were indicated that pH value is higher in mare milk and acidity and viscosity is lower in mare  milk than those in cow milk. A hygienically obtained mare milk samples were used in this experiment.  Acidity, pH and Total Bacterial Counts were monitored with the reference to raw horse milk standard  of Mongolia. The results were indicated that hygienically obtained mare milk can be kept for 12 hours  at room temp. In case of refrigerated condition it kept its acceptable freshness up 10 days of storage.  Монгол гүүний саамны гарц, найрлага, физик-химийн шинж чанар, шинэлэг байдлыг тодорхойлсон дүн Хураангуй: Монгол гүүний саалийн хугацаанд (6-10-р саруудад) саамны гарц, найрлага түүний физик-химийн үзүүлэлт, хадгалалтын нөхцөл саамны шинэлэг байдалд хэрхэн нөлөөлөхийгтодорхойлох зорилгоор энэхүү судалгааны ажлыг хийж гүйцэтгэлээ. Судалгаанд 7-12 насны 7 гүүг ашиглав. Саамны гарц лактацийн 90 дэх хоногт хамгийн өндөр,120 болон 150 дах хоногуудад гарцын хэмжээ буурсан хэдий ч лактозын агууламж тогтворжиж байв. Физик-химийн зарим үзүүлэлтийг тодруулахын тулд үнээний сүүтэй харьцуулахад гүүний саам нь исгэлэн, рН, өнгө, урсанги чанар зэрэг үзүүлэлтүүдээр ялгаатай байв. Саамыг хадгалалтын хоёр өөр нөхцөл, хэмд хадгалж туршихад тасалгааны нөхцөлд 12 цаг хүртэл, хөргүүрийн нөхцөлд 10 хоног хүртэл шинэлэг байдал нь хадгалагдаж чадаж байлаа. Түлхүүр үг: Хадгалалт, саамны өнгө, саамны уураг, саамны лактоз


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