Investigation of Antimicrobial Activity of Encapsulated Essential Oils

2007 ◽  
Vol 555 ◽  
pp. 429-434 ◽  
Author(s):  
Marko Rakin ◽  
Lj. Mojović ◽  
Suzana Dimitrijević ◽  
K. Mihajlovski ◽  
S. Siler Marinković
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Rosmarinus Officinalis ◽  
Yeast Cells ◽  
Bacterial Cells ◽  
Active Components ◽  
Abies Sibirica ◽  
Alginate Capsules ◽  
Long Time ◽  
Medical Textile

Antimicrobial activity of essential oils obtained from many plants has been known for a long time. However, the use of essential oils as active components of biomedical textile have recently gained popularity and aroused scientific interest. The antimicrobial activity of two essential oils, Rosmarinus officinalis and Abies sibirica, respectively, was detected using two indicator strains: Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922, respectively. To achieve a controlled release of antimicrobial activity and to enable utilization of higher concentrations of active ingredient, the essential oils were first encapsulated in beads based on alginate, gelatin and yeast cells, and then bounded to medical textile. The maximum oil content (85.4%) was achieved in alginate capsules. The release of essential oils was followed by determination of viable bacterial cells during a seven-day incubation of beads in saline. Rosmarinus officinalis and Abies sibirica immobilized in the beads showed a significantly prolonged activity, with some gel-dependent variation.

scholarly journals Impact of Altitudinal Variation on the Phytochemical Profile, Anthelmintic and Antimicrobial Activity of Two Pinus Species

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3170
Author(s):  
Wafaa M. Elkady ◽  
Mariam H. Gonaid ◽  
Miriam F. Yousif ◽  
Mahmoud El-Sayed ◽  
Hind A. N. Omar
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Pinus Halepensis ◽  
Biological Activities ◽  
Anthelmintic Activity ◽  
Antimicrobial Activities ◽  
Pinus Pinea ◽  
Active Components ◽  
Pinus Species ◽  
Bacteria And Fungi

Active components from natural sources are the current focus in most pharmacological research to provide new therapeutic agents for clinical use. Essential oils from the Pinus species have been traditionally used in medicine. This study aimed to investigate the chemical profile of two Pinus species, Pinus halepensis L. and Pinus pinea Mill, from different altitudes in Libya and study the effect of environmental conditions on the biological activities of essential oils. A clevenger apparatus was used to prepare the essential oils by hydrodistillation. Analyses were done using GC/MS. Anthelmintic and antimicrobial activities were tested against the earthworm Allolobophora caliginosa, gram-positive bacteria, gram-negative bacteria, and fungi. Different chemical profiles were observed among all tested essential oils, and terpenes were the most dominant class. All studied essential oils from the Pinus species exhibited a remarkable anthelmintic activity compared to the standard piperazine citrate drug. Pinus halepensis from both altitudes showed broad-spectrum antimicrobial activity against all tested microorganisms, while Pinus pinea was effective against only Escherichia coli. From these findings, one can conclude that there are variations between studied species. The essential oil compositions are affected by environmental factors, which consequently affect the anthelmintic and antimicrobial activity.

scholarly journals Allium sativum, Rosmarinus officinalis, and Salvia officinalis Essential Oils: A Spiced Shield against Blowflies

Insects ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 143 ◽  
Author(s):  
Stefano Bedini ◽  
Salvatore Guarino ◽  
Maria Cristina Echeverria ◽  
Guido Flamini ◽  
Roberta Ascrizzi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Foodborne Pathogens ◽  
Allium Sativum ◽  
Insect Pests ◽  
Meat Products ◽  
Rosmarinus Officinalis ◽  
Salvia Officinalis ◽  
Meat Processing ◽  
Toxic Activity

Blowflies are known vectors of many foodborne pathogens and unintentional human ingestion of maggots by meat consumption may lead to intestinal myiasis. In fact, the control of insect pests is an important aspect of industrial and home-made food processing and blowflies (Diptera: Calliphoridae), which are among the most important pests involved in the damage of meat products. Most spices, largely used in food preparations and industry, contain essential oils that are toxic and repellent against insects and exert antimicrobial activity. In this study, we assessed the electro-antennographic responses, the oviposition deterrence, the toxicity, and the repellence of the essential oils (EOs) of Allium sativum L., Salvia officinalis L., and Rosmarinus officinalis L. against the blowfly Calliphora vomitoria L. We tested the EOs antibacterial and antifungal properties and the efficacy of an A. sativum EO-charged mist sprayed in the tunnel entryway of a meat processing room to form an olfactive barrier against the entrance of flies. The results showed that the EOs are perceived by female blowfly’ antennae and exert an evident repellent activity against them completely deterring the oviposition for up to 24 h starting from the concentration of 2.5 μL cm−2 EO. The EOs also exhibited toxic activity by both topical application (LD50 from 0.44 to 1.97 μL insect−1) and fumigation (LC50 from 1.76 to 31.52 μL L−1) against adults of C. vomitoria and were able to exert a clear antimicrobial activity toward pathogens. Lastly, the EO-charged mist was able to reduce by about 40% the presence of Calliphoridae in the meat processing room of a dry-ham factory.

scholarly journals Essential Oils with High Activity against Stationary Phase Bartonella henselae

Antibiotics ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 246 ◽  
Author(s):  
Xiao Ma ◽  
Wanliang Shi ◽  
Ying Zhang
Keyword(s):  
Stationary Phase ◽  
Essential Oils ◽  
High Activity ◽  
High Throughput Screening ◽  
Bartonella Henselae ◽  
Current Treatment ◽  
Bacterial Cells ◽  
Active Components ◽  
Chronic Infections ◽  
Cinnamon Bark

Bartonella henselae is a fastidious Gram-negative intracellular bacterium that can cause cat scratch disease, endocarditis in humans and animals, as well as other complications, leading to acute or chronic infections. The current treatment for Bartonella infections is not very effective presumably due to bacterial persistence. To develop better therapies for persistent and chronic Bartonella infections, in this study, with the help of SYBR Green I/PI viability assay, we performed a high-throughput screening of an essential oil library against the stationary phase B. henselae. We successfully identified 32 essential oils that had high activity, including four essential oils extracted from Citrus plants, three from Origanum, three from Cinnamomum, two from Pelargonium, and two from Melaleuca, as well as frankincense, ylang-ylang, fir needle, mountain savory (winter), citronella, spearmint, elemi, vetiver, clove bud, allspice, and cedarwood essential oils. The minimal inhibitory concentration (MIC) determination of these 32 top hits indicated they were not only active against stationary phase non-growing B. henselae but also had good activity against log-phase growing B. henselae. The time-kill assay showed 13 active hits, including essential oils of oregano, cinnamon bark, mountain savory (winter), cinnamon leaf, geranium, clove bud, allspice, geranium bourbon, ylang-ylang, citronella, elemi, and vetiver, could eradicate all stationary phase B. henselae cells within seven days at the concentration of 0.032% (v/v). Two active ingredients, carvacrol and cinnamaldehyde, of oregano and cinnamon bark essential oils, respectively, were shown to be very active against the stationary phase B. henselae such that they were able to eradicate all the bacterial cells even at the concentration ≤ 0.01% (v/v). More studies are needed to identify the active components of some potent essential oils, decode their antimicrobial mechanisms, and evaluate their activity against Bartonella infections in animal models.

scholarly journals CHEMICAL COMPOSITION OF ESSENTIAL OILS FROM PANTELLERIA ISLAND AUTOCHTHONOUS AND NATURALIZED SPICES AND EVALUATION OF THEIR INDIVIDUAL AND COMBINED ANTIMICROBIAL ACTIVITIES

2019 ◽  
pp. 45-58
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Antimicrobial Activities ◽  
Rosmarinus Officinalis ◽  
Minimum Inhibitory Concentrations ◽  
Food Borne Pathogens ◽  
Food Borne ◽  
Rosmarinus Officinalis L ◽  
Origanum Majorana

In this study, the antimicrobial activity of the essential oils (EOs) from Origanum majorana L. and Rosmarinus officinalis L. growing in Pantelleria (Sicily, Italy) were tested alone and in combination against some prokaryotic and eukaryotic food-borne pathogens. The chemical composition of the EOs as well as the minimum inhibitory concentrations (MIC) against the most sensitive strains were also determined. Both EOs showed interesting antimicrobial effects against all bacteria and yeasts tested. MIC was in the range 1.25–2.50 µl/ml. Interestingly, O. majorana was particularly rich in thymol acetate, while carvacrol was present at very low percentages. Also R. officinalis EOs composition was different from rosemary collected in different areas, as being particularly rich in caryophyllene. Furthermore, the antimicrobial activity of the combination of O. majorana and R. officinalis EOs indicated their potential as food biopreservatives.

Antibacterial Activity of Rosmarinus officinalis and Lavandula angustifolia Essential Oils Against Selected Poultry Pathogenic Bacteria

2021 ◽  
Vol 1 (30) ◽  
pp. 49-57
Author(s):  
F.Moukhfi N.Chadli
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Antioxidant Properties ◽  
Rosmarinus Officinalis ◽  
Poultry Feed ◽  
Gas Chromatography Mass Spectrometry ◽  
Bacterial Strains ◽  
Lavandula Angustifolia

Abstract- Poultry is the host of many species of bacteria and the intestine is the privileged place of their colonization and their persistence. These bacteria are the cause of several cases of food poisoning in humans through the consumption of eggs or soiled chicken meat. In addition, these bacteria develop resistance to antibiotics that are adjusted to poultry feed as growth promoters. Essential oils are considered as important secondary metabolites for plant defense by their antimicrobial and antioxidant properties. These essential oils may be considered as a source of natural antimicrobials for the conservation of poultry food. The aim of our study is to isolate and identify bacterial strains isolated from poultry and to determine the antibacterial and antioxidant activities of Rosmarinus officinalis and Lavandula angustifolia essential oils on these bacterial strains.Essential oils of Moroccan Rosmarinus officinalis and Lavandula angustifolia were extracted by hydrodistillation. The identification of their chemical composition are performed by gas chromatography-mass spectrometry. Antimicrobial activity of extracted essential oils against Staphylococcus aureus, Clostridium perfringens, Escherichia coli and Salmonella entiritidis was evaluated by aromatogram test and Microdilution in a liquid medium. The identification of strains are performed by several test: Gram staining, Kligler test, Catalase test, test Mobility and Api Gallery 20E. The results show that essential oils tested have a considerable antibacterial activity against all isolated bacterial strains. Keywords: Poultry feed, Essential oils, Bacterial strains, Antibiotic resistance, Antimicrobial activity.

scholarly journals Antimicrobial Activity of Blended Essential Oil Preparation

2012 ◽  
Vol 7 (10) ◽  
pp. 1934578X1200701 ◽  
Author(s):  
Sarin Tadtong ◽  
Supatcha Suppawat ◽  
Anchalee Tintawee ◽  
Phanida Saramas ◽  
Suchada Jareonvong ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Volume Ratio ◽  
Antimicrobial Effect ◽  
Antimicrobial Activities ◽  
Active Components ◽  
Linalyl Acetate ◽  
Lavender Oil ◽  
Agar Disc

Antimicrobial activities of two blended essential oil preparations comprising lavender oil, petigrain oil, clary sage oil, ylang ylang oil and jasmine oil were evaluated against various pathogenic microorganisms. Both preparations showed antimicrobial activity in the agar disc diffusion assay against the Gram-positive bacteria, Staphylococcus aureus ATCC6538 and S. epidermidis isolated strain, the fungus, Candida albicans ATCC10231, and the Gram-negative bacterium, Escherichia coli ATCC25922, but showed no activity against Pseudomonas aeruginosa ATCC9027. The minimum inhibitory concentration (MIC) of these preparations was evaluated. By the broth microdilution assay, preparation 1, comprising lavender oil, clary sage oil, and ylang ylang oil (volume ratio 3:4:3), exhibited stronger antimicrobial activity than preparation 2, which was composed of petigrain oil, clary sage oil, and jasmine oil (volume ratio 3:4:3). Moreover, the sum of the fractional inhibitory concentrations (Σfic) of preparation 1 expressed a synergistic antimicrobial effect against the tested microorganisms (Σfic<1). The blended essential oil preparations, characterized for their components by GC/MS, contained linalyl acetate, and linalool as major components. Our experiments showed that the differential antimicrobial effect of either blended oil preparations or single/pure essential oils may be influenced by the amount of linalool and linalyl acetate, and the number of active components in either the blended preparations or single/pure essential oils. In addition, blended oil preparations expressed synergistic antimicrobial effect by the accumulation of active components such as linalool and linalyl acetate and combining active constituents of more than one oil.

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