Effect of TiO2 and SiO2 on Surface Reactivity of Calcium Phosphate Glasses in Simulated Body Fluid

2008 ◽  
Vol 587-588 ◽  
pp. 138-142 ◽  
Author(s):  
A.M.B. Silva ◽  
Rui N. Correia ◽  
J.M. Oliveira ◽  
Maria Helena F.V. Fernandes
Keyword(s):  
Simulated Body Fluid ◽  
Inductively Coupled Plasma ◽  
Body Fluid ◽  
Spectroscopic Studies ◽  
Surface Reactivity ◽  
Phosphate Glasses ◽  
Inductively Coupled ◽  
System A ◽  
Dissolution Behaviour

This study compares the in vitro behaviour in SBF of glasses from two different systems, TiO2-CaO-P2O5 and SiO2-CaO-P2O5 with the same TiO2 and SiO2 molar content, in order to evaluate the effect of TiO2 and SiO2 on the surface reactivity of those glasses. The glass formation regions in both systems were observed for compositions with less than 40 mol% TiO2 and 40 mol% SiO2, respectively. Four glasses with similar TiO2 and SiO2 molar contents have been selected for comparison. These glasses are equimolar in CaO and P2O5 with TiO2 or SiO2 varying from 4 to 33 mol %. Powder glasses were immersed in Simulated Body Fluid (SBF) and kept at 37°C for different times, up to 14 days. Surfaces were observed by Scanning Electron Microscopy (SEM) and specimen ion leaching to SBF was studied by Inductively Coupled Plasma (ICP) spectroscopy. Preliminary spectroscopic studies by Raman were performed to identify the structure of the glasses. For glasses of the SiO2-CaO-P2O5 system a significant dissolution of all ions was observed together with the formation of phosphoric acid. In opposition, the immersion of TiO2-CaO-P2O5 glasses produced a small Ca consumption and stable Ti and P concentrations, indicating the formation of a Ca-P rich layer on these glasses. The observed differences in the dissolution behaviour are tentatively explained in terms of the glass structures obtained by spectroscopy.

scholarly journals Metal ions leachables from fake orthodontic braces incubated in simulated body fluid

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Riyam Haleem ◽  
Noor Ayuni Ahmad Shafiai ◽  
Siti Noor Fazliah Mohd Noor
Keyword(s):  
Simulated Body Fluid ◽  
Metal Ions ◽  
Inductively Coupled Plasma ◽  
Metal Ion ◽  
Body Fluid ◽  
Atomic Emission ◽  
Analysis Data ◽  
Inductively Coupled ◽  
Significant Release ◽  
Control Samples

Abstract Background The demand for fake braces usage in Southeast Asia are increasing but lack of certification and information on fake braces as medical devices from regulated bodies raised a concern towards its safety. The aim of this study was to determine the types of metal ion leachable from removable fake braces based on heavy metal ions present in metallic materials, immersed in simulated body fluid (SBF) and analysed using inductively coupled plasma atomic emission spectroscopy. Methods Three sets of fake braces and one control were dissembled to only their brackets and archwires and immersed separately in SBF. They were placed in an incubator shaker at a temperature of 37 °C at 50 rpm. A 3.0 ml measurement of SBF was taken out from the sample containers at days 7, 14 and 28 and kept at − 20 °C for further analysis. Data were analysed using SPSS version 26.0 (IBM, Armonk, USA) (P < 0.05). Descriptive and one-way ANOVA analyses with Bonferroni post hoc tests were used to assess the significant differences between the metal ions released in SBF from the control samples and fake braces. Results All 23 elements under investigation except Si ions were detected from the control samples and fake braces. There were significant increased K ions and reduced levels of Mg ions from the fake archwires and brackets. Most ions released were less than 10 mg/L (Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Mo, Cd, Pb, Al) or 1 mg/L (Li, Ba) into the SBF medium. Conclusion There were significant release of Ca and K ions from the fake samples. Elements such as Li, Ba, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Mo, Cd and Sb had increased in concentration at day 7 and the concentration plateaued until day 28.

IN VITRO OSTEO-INDUCTIVE CHARACTERIZATION OF NANO-GRADE PEARL POWDERS

2011 ◽  
Vol 23 (02) ◽  
pp. 135-140
Author(s):  
Mei-Ju Hou ◽  
Chi-Jen Shih
Keyword(s):  
Inductively Coupled Plasma ◽  
Body Fluid ◽  
X Ray Diffraction ◽  
X Ray ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Hydroxyl Apatite ◽  
Inductive Behavior

The main objective of this study is to characterize the in vitro osteo inductive behavior of pearl nano crystallites. The results obtained from X-ray diffraction, Fourier transform infrared (FTIR) spectra, and inductively coupled plasma mass (ICP-MS) analysis demonstrate that the pearls can induce the formation of a hydroxyl apatite (HA) layer on their surface in simulated body fluid (SBF), even after only short soaking periods. Further, MC3T3-E1 cells can easily attach and spread on the pearl powders after 1 h of cultivation.

IN-VITRO BEHAVIOUR OF BIPHASIC CALCIUM PHOSPHATE WITH DIFFERENT RATIO OF SILICA CONTENT IN SIMULATED BODY FLUID

2015 ◽  
Vol 23 (1) ◽  
pp. 1-14
Author(s):  
Sudirman Sahid ◽  
◽  
Nor Shahida Kader Bashah ◽  
Salina Sabudin ◽  
◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Simulated Body Fluid ◽  
Body Fluid ◽  
Biphasic Calcium Phosphate ◽  
Silica Content

scholarly journals Tetracalcium Phosphate/Monetite/Calcium Sulfate Hemihdrate Biocement Powder Mixtures Prepared by the One-Step Synthesis for Preparation of Nanocrystalline Hydroxyapatite Biocement-Properties and In Vitro Evaluation

Materials ◽  
2021 ◽  
Vol 14 (9) ◽  
pp. 2137
Author(s):  
Lubomir Medvecky ◽  
Maria Giretova ◽  
Radoslava Stulajterova ◽  
Lenka Luptakova ◽  
Tibor Sopcak
Keyword(s):  
Simulated Body Fluid ◽  
Body Fluid ◽  
Calcium Sulfate ◽  
Powder Mixtures ◽  
Liquid Component ◽  
Tetracalcium Phosphate ◽  
Nanocrystalline Hydroxyapatite ◽  
One Step ◽  
Calcium Sulfate Hemihydrate

A modified one-step process was used to prepare tetracalcium phosphate/monetite/calcium sulfate hemihydrate powder cement mixtures (CAS). The procedure allowed the formation of monetite and calcium sulfate hemihydrate (CSH) in the form of nanoparticles. It was hypothesized that the presence of nanoCSH in small amounts enhances the in vitro bioactivity of CAS cement in relation to osteogenic gene markers in mesenchymal stem cells (MSCs). The CAS powder mixtures with 15 and 5 wt.% CSH were prepared by milling powder tetracalcium phosphate in an ethanolic solution of both orthophosphoric and sulfuric acids. The CAS cements had short setting times (around 5 min). The fast setting of the cement samples after the addition of the liquid component (water solution of NaH2PO4) was due to the partial formation of calcium sulfate dihydrate and hydroxyapatite before soaking in SBF with a small change in the original phase composition in cement powder samples after milling. Nanocrystalline hydroxyapatite biocement was produced by soaking of cement samples after setting in simulated body fluid (SBF). The fast release of calcium ions from CAS5 cement, as well as a small rise in the pH of SBF during soaking, were demonstrated. After soaking in SBF for 7 days, the final product of the cement transformation was nanocrystalline hydroxyapatite. The compressive strength of the cement samples (up to 30 MPa) after soaking in simulated body fluid (SBF) was comparable to that of bone. Real time polymerase chain reaction (RT-PCR) analysis revealed statistically significant higher gene expressions of alkaline phosphatase (ALP), osteonectin (ON) and osteopontin (OP) in cells cultured for 14 days in CAS5 extract compared to CSH-free cement. The addition of a small amount of nanoCSH (5 wt.%) to the tetracalcium phosphate (TTCP)/monetite cement mixture significantly promoted the over expression of osteogenic markers in MSCs. The prepared CAS powder mixture with its enhanced bioactivity can be used for bone defect treatment and has good potential for bone healing.

scholarly journals In Vitro Ion Release of Wires in Removable Orthodontic Appliances

Materials ◽  
2021 ◽  
Vol 14 (12) ◽  
pp. 3402
Author(s):  
Lena Wepner ◽  
Harald Andreas Färber ◽  
Andreas Jaensch ◽  
Anna Weber ◽  
Florian Heuser ◽  
...  
Keyword(s):  
Mechanical Loading ◽  
Corrosive Medium ◽  
Inductively Coupled Plasma ◽  
Petri Dish ◽  
Orthodontic Appliances ◽  
Testing Time ◽  
Ion Release ◽  
Inductively Coupled ◽  
Corrosive Solution

Various orthodontic wire compositions and configurations are present on the market for removable appliances; however, there have still been only few studies focusing on the effect of resin color and additives such as glitter on corrosion of metallic wires under different conditions. Thus, the aim of the study was to compare concentrations of released ions (aluminium, chromium, nickel) in a corrosive medium under three different conditions: non-loaded wires, loaded wires, and non-loaded wires treated with Kukis® cleaning tablets. Six different wires made of three types of steel alloy were embedded in PMMA resin leaving one centimetre of each wire emerging from the resin to come into contact with the corrosive medium. Glitter particles were added to half of the produced test specimens. For the unloaded test series, five specimens of each group were covered in a petri dish with 50 mL of corrosive medium (pH 2.3) following EN-ISO 10271 for seven days at 37 °C. The wires for the mechanically loaded test specimens overlapped the resin by 5 cm and were clamped into a time-switched electric drive for a defined period of time before the samples were taken after a testing time of 7 days. In the third group, unloaded test specimens were transferred from their petri dishes into the prepared Kukis® solution every 24 h before being stored in the corrosive medium. Inductively coupled plasma mass spectrometry (ICP-MS) was used to quantify the specific ions in the corrosive solution. Statistical analysis showed that the mechanical loading of all wires could significantly raise the diffusion of ions into the corrosive medium. The colour of the resin did not affect the concentration of the released ions. The Kukis® cleaning tabs could not lower the corrosion of the tested metals, as some of the wires were corroded even more using the brace cleanser. Glitter-containing test specimens showed significantly higher amounts of aluminium. Mechanical loading as well as the presence of glitter particles in the resin significantly affected ion concentrations.

scholarly journals Preparation and Degradation Characteristics of MAO/APS Composite Bio-Coating in Simulated Body Fluid

Coatings ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 667
Author(s):  
Zexin Wang ◽  
Fei Ye ◽  
Liangyu Chen ◽  
Weigang Lv ◽  
Zhengyi Zhang ◽  
...  
Keyword(s):  
Simulated Body Fluid ◽  
Composite Coating ◽  
Body Fluid ◽  
Composite Coatings ◽  
Degradation Process ◽  
Ceramic Coatings ◽  
Immersion Test ◽  
Substrate Material ◽  
Atmospheric Plasma

In this work, ZK60 magnesium alloy was employed as a substrate material to produce ceramic coatings, containing Ca and P, by micro-arc oxidation (MAO). Atmospheric plasma spraying (APS) was used to prepare the hydroxyapatite layer (HA) on the MAO coating to obtain a composite coating for better biological activity. The coatings were examined by various means including an X-ray diffractometer, a scanning electron microscope and an energy spectrometer. Meanwhile, an electrochemical examination, immersion test and tensile test were used to evaluate the in vitro performance of the composite coatings. The results showed that the composite coating has a better corrosion resistance. In addition, this work proposed a degradation model of the composite coating in the simulated body fluid immersion test. This model explains the degradation process of the MAO/APS coating in SBF.

scholarly journals Chemistry of Tropical Eucheumatoids: Potential for Food and Feed Applications

Biomolecules ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 804
Author(s):  
Andrea Ariano ◽  
Nadia Musco ◽  
Lorella Severino ◽  
Anna De Maio ◽  
Annabella Tramice ◽  
...  
Keyword(s):  
Chemical Analysis ◽  
Inductively Coupled Plasma ◽  
Mineral Content ◽  
Kappaphycus Alvarezii ◽  
Optical Emission ◽  
Essential Elements ◽  
Feed Additive ◽  
Inductively Coupled ◽  
Food And Feed

The use of seaweeds as additives in animal nutrition may be a valid option to traditional feed as they represent a rich source of minerals, carbohydrates and antioxidants. The aim of this study was to analyze the chemical composition and in vitro antioxidant capacity of two tropical eucheumatoids, Kappaphycus alvarezii and Kappaphycus striatus, in Malaysian wild offshore waters. The chemical analysis was performed via inductively coupled plasma–optical emission spectroscopy for evaluating the concentration of toxic (Cd, Pb, Hg, As) and essential elements (Mn, Fe, Cu, Ni, Zn, Se); NMR spectroscopy was used for carrageenans investigation. Furthermore, the soluble and fat-soluble antioxidant capacities were determined by FRAP, DPPH and ABTS assays. The chemical analysis revealed a higher content of trace elements in K. alvarezii as compared to K. striatus, and both exhibited a high mineral content. No significant differences in metal concentrations were found between the two species. Both samples showed a mixture of prevailing κ- and t-carrageenans. Finally, the levels of soluble and fat-soluble antioxidants in K. alvarezii were significantly higher than in K. striatus. Our findings suggest that K. alvarezii could be used as a potential feed additive because of its favorable chemical and nutritional features.

scholarly journals DNA-Platinum Thin Films for Use in Chemoradiation Therapy Studies

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Mohammad Rezaee ◽  
Elahe Alizadeh ◽  
Darel Hunting ◽  
Léon Sanche
Keyword(s):  
Inductively Coupled Plasma ◽  
Reaction Times ◽  
Substantial Effect ◽  
Chemotherapeutic Agents ◽  
Chemoradiation Therapy ◽  
Inductively Coupled ◽  
Dna Films ◽  
Effects Of Radiation ◽  
Dna Platination

Dry films of platinum chemotherapeutic drugs covalently bound to plasmid DNA (Pt-DNA) represent a useful experimental model to investigate direct effects of radiation on DNA in close proximity to platinum chemotherapeutic agents, a situation of considerable relevance to understand the mechanisms underlying concomitant chemoradiation therapy. In the present paper we determine the optimum conditions for preparation of Pt-DNA films for use in irradiation experiments. Incubation conditions for DNA platination reactions have a substantial effect on the structure of Pt-DNA in the films. The quantity of Pt bound to DNA as a function of incubation time and temperature is measured by inductively coupled plasma mass spectroscopy. Our experiments indicate that chemical instability and damage to DNA in Pt-DNA samples increase when DNA platination occurs at37°Cfor 24 hours, the condition which has been extensively used for in vitro studies. Platination of DNA for the formation of Pt-DNA films is optimal at room temperature for reaction times less than 2 hours. By increasing the concentration of Pt compounds relative to DNA and thus accelerating the rate of their mutual binding, it is possible to prepare Pt-DNA samples containing known concentrations of Pt while reducing DNA degradation caused by more lengthy procedures.

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