scholarly journals PHYTOCHEMICAL ANALYSIS AND ANTIBACTERIAL ACTIVITY OF ERYNGIUM PYRAMIDALE BOISS. & HAUSSKN

2021 ◽  
Vol 66 (2) ◽  
pp. 5230-5236
Author(s):  
Mohammad Nejati ◽  
Shiva Masoudi ◽  
Dara Dastan ◽  
Nasrin Masnabadi
2011 ◽  
Vol 3 (12) ◽  
pp. 57-59 ◽  
Author(s):  
K. Riazunnisa K. Riazunnisa ◽  
◽  
U. Adilakshmamma U. Adilakshmamma ◽  
C. Habeeb khadri

2021 ◽  
Vol 2 (1) ◽  
pp. 23-33
Author(s):  
Abdul Aziz ◽  
Veggy Nadya Yuliawan ◽  
Paula Mariana Kustiawan

Propolis is one of the natural products produced by kelulut bees and is still not widely used. The type of stingless bee that is the prima donna in the community is Heterotrigona itama. This study aims to determine the phytochemical content of the n-hexane fraction of Heterotrigona itama bee propolis collected from Kutai Kartanegara, East Kalimantan. The n-hexane fraction was obtained from the methanol extract of H. itama propolis by the liquid-liquid partition method. After obtaining the n-hexane fraction, the research continued with a qualitative phytochemical test to identify the compound and determine total phenolic. Antibacterial activity was determined by the agar well diffusion method with a serial concentration in Escherichia coli bacteria. Qualitative phytochemical analysis in the form of color changes showed that the n-hexane fraction of H. itama propolis contained flavonoids, alkaloids, saponins, and tannins. Based on the results, the total phenolic content of the n-hexane fraction sample was 490 mgGAE/100 g. It caused the n-hexane fraction to have lower phenolic content than the methanol extract (792 mg GAE100 g). Furthermore, this result indicated that the non-polar fraction was not substantial enough to extracted phenolic compounds. It correlated to the antibacterial activity of the n-hexane fraction, which was very weak (2  mm ± 1.5) at  200µg/mL concentration.


Author(s):  
Dhanapaul Vinoth Kumar

The present study was designed to evaluate the phytochemical analysis and antibacterial activity of Swietenia mahagoni leaf extract. The leaf extract was subjected to a variety of phytochemical analysis. The qualitative phytochemical tests exhibited the presence of common phytocompounds like alkaloids, tannins, saponins, phlobatannins, flavanoids, terpenoids and volatile oils as major active constituents. The plant (Swietenia mahagoni) leaf has valuable medicinal uses, hence petroleum ether (18.2g) was found to be a good solvent for the phytochemical and antibacterial study. Similarly, the Methanolic leaf extract of Swietenia mahagoni has highest zone of inhibition on Bacillus subtilis.


Author(s):  
SHUSHIL BHUSAL ◽  
KHAGA RAJ SHARMA

Objectives: The present study was designed to investigate the phytochemical analysis, antioxidant potential, and antibacterial activities of the traditionally used medicinal plant Glycyrrhiza glabra. Methods: The plant secondary metabolites were extracted through cold percolation using methanol (MeOH) as a solvent. The MeOH extract was further fractionated in different solvents in increasing order of polarity. The antioxidant activity was evaluated by 2,2-diphenyl-1-picrylhydrazyl assay. The antibacterial activity was studied by agar well diffusion method. Results: The antioxidant potential IC50 was found 43.13, 104.83, and 200.11 μg/ml for ethyl acetate (EtOAc), MeOH, and chloroform (CHCl3) extracts, respectively. The EtOAc fraction showed the potent antioxidant with IC50 43.13 μg/ml compared to the standard ascorbic acid 58.76 μg/ml. The antimicrobial activity exhibited by MeOH extract against Bacillus subtilis (ATCC 6051) and Staphylococcus aureus (ATCC 6538P) zone of inhibition was 18 mm and 17 mm, for chloroform extracts 15 mm and 13 mm, and for EtOAc fraction 11 mm against Bacillus subtilis. The highest dilution that yielded no single bacteria colony on the nutrient agar plates for Bacillus subtilis and S. aureus of MeOH extract was found 0.39 mg/ml and 6.25 mg/ml, for chloroform extract 3.125 mg/ml and 6.25 mg/ml and EtOAc fraction against Bacillus subtilis was 12.50 mg/ml as minimum bactericidal concentration. Conclusion: The plant extracts showed potent antioxidant and antibacterial activity. The results support for using the G. glabra in bacterial infection which provides partial scientific validation for using the plant against bacterial infections.


2014 ◽  
Author(s):  
Muhammad Muzammal

Comparative study of plant extracts crude and aqueous, methanolic and ethanolic with antibiotics, provide evidence that calotropis procera extracts has the similar antibacterial activity as these antibiotics against test pathogens i.e. Salmonella typhi and E.coli. The analysis of antimicrobial activity of aqueous, methanolic and ethanolic extract of leaves and flower of Calotropis procera was carried out in disc method and also determined MIC value at 600nm through optical density using spectrophotometer. The zone of inhibition produced by extracts was examined and compares it with zone produced by antibiotics. The effect exhibited by ethanolic extract of leaves and flower was significantly greater than the aqueous and methanolic extract of leaves and flower. Crude extracts i.e. latex, leaves, fruit and flower crude extracts. Among them, flower crude extracts shows similar zone of inhibition to test anitibiotics.while in MIC value, we made different concentration of extracts and antibiotics.i.e for crude we made 25%, 50%.75% and 100% concentration of crude juice and for the aqueous, methanolic and ethanolic we made 0.5mg/mL,1mg/mL,2mg/mL,3mg/mL,4mg/mL, 5mg/mL and 6mg/mL in Dimethyl salfoxide, and same antibiotics concentration. Aqueous leaves extracts show MIC at 0.5mg/ml against E.coli while against Salmonella it shows MIC at 1mg/ml... We also determine phytochemical analysis for presences of different compounds in crude extracts. The obtained results provide a support for the use of Calotropis procera, in traditional medicine and suggest its further advance investigation.


2016 ◽  
Vol 8 (3) ◽  
pp. 333 ◽  
Author(s):  
Abdullahi Aliyu ◽  
Alkali BR ◽  
Yahaya MS ◽  
Garba A ◽  
Adeleye SA ◽  
...  

<p>The aqueous and ethanol extracts of the bark of<em> Khaya senegalensis</em> were screened for their phytochemical constituents and preliminary antibacterial activity against <em>Bacillus subtilis, Escherichia coli</em> and<em> Proteus mirabilis. </em>The minimum inhibitory concentration (MIC) of the plant on the tested organisms was determined using multiple tubes method.</p><p>Alkaloids, anthraquinones, glycosides, tannins and steroids were detected in both extracts.</p><p>The ethanol and aqueous extracts of the plant showed antibacterial activity against <em>B. subtilis and E. coli,</em> with the aqueous extracts having more activity than those of ethanol. However the growth of<em> P. mirabilis</em> was not inhibited by either of the extracts. The MIC value was determined to be 50 mg/ml for<em> B. subtilis </em>and<em> E. coli. </em>The results are suggestive of considerable antibacterial activity of<em> K. senegalensis </em>and may justify its use in the treatment of bacterial diseases by herbalists or traditional healers.</p>


2008 ◽  
Vol 9 (1) ◽  
pp. 63-67 ◽  
Author(s):  
U.A. Stephen ◽  
F. Abiodun ◽  
O. Osahon ◽  
E. Ewaen

2013 ◽  
Vol 16 (19) ◽  
pp. 984-990 ◽  
Author(s):  
Kenza Kadi ◽  
Abdelouahab Yahia ◽  
Sofia Hamli ◽  
Laiche Auidane ◽  
Hamid Khabthane ◽  
...  

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