scholarly journals In vitro comparison of antimicrobial effect of sodium hypochlorite solution and Zataria multiflora essential oil as irrigants in root canals contaminated with Candida albicans

2016 ◽  
Vol 19 (1) ◽  
pp. 101 ◽  
Author(s):  
Mahdi Sedigh-Shams ◽  
Parisa Badiee ◽  
Alireza Adl ◽  
MiladDadollahi Sarab ◽  
Abbas Abbaszadegan ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Essential Oil ◽  
Sodium Hypochlorite ◽  
Antimicrobial Effect ◽  
Sodium Hypochlorite Solution ◽  
Zataria Multiflora ◽  
Hypochlorite Solution ◽  
Root Canals ◽  
In Vitro Comparison

scholarly journals The Antibacterial Efficacy of Biopure MTAD in Root Canal Contaminated with Enterococcus faecalis

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Blerim Kamberi ◽  
Donika Bajrami ◽  
Miranda Stavileci ◽  
Shuhreta Omeragiq ◽  
Fatmir Dragidella ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Sodium Hypochlorite Solution ◽  
Antibacterial Efficacy ◽  
Human Teeth ◽  
Exact Test ◽  
Hypochlorite Solution ◽  
Root Canals ◽  
Significant Difference ◽  
Level Of Significance

Aim. The purpose of this in vitro study was to assess the antimicrobial efficacy of Biopure MTAD against E. faecalis in contaminated root canals. Materials and Methods. Forty-two single rooted extracted human teeth were inoculated with E. faecalis and incubated for four weeks. The samples were divided in two control and five experimental groups irrigated with 1.5% sodium hypochlorite solution (NaOCl); 3% NaOCl; BioPure MTAD; 1.5% NaOCl/17% EDTA; or 3% NaOCl/17% EDTA. After a one-week incubation, complete disinfection was confirmed by the absence of turbidity in the incubation media. Dentin shavings were taken from samples with no turbidity to verify whether E. faecalis was present in dentin tubules. Results were analyzed statistically using Fisher's exact test, with the level of significance set at . Results. Statistical analysis of the data obtained at Day 7 and after dentin shaving analysis showed that BioPure MTAD had significantly greater antibacterial activity than 1.5% NaOCl, 1.5% NaOCl/17% EDTA and 3% NaOCl/17% EDTA. No significant difference was detected between MTAD and 3% NaOCl. Conclusions. These findings suggest that BioPure MTAD possesses superior bactericidal activity compared with NaOCl and EDTA against E. faecalis.

scholarly journals IN VITRO EVALUATION OF ANTIMICROBIAL EFFICACY OF DIODE LASER WHEN USED ADJUNCTIVELY WITH SODIUM HYPOCHLORITE, METHYLENE BLUE OR TOLUIDINE BLUE IN ENTEROCOCCUS FAECALIS CONTAMINATED ROOT CANALS

2020 ◽  
pp. 148-151
Author(s):  
N. Shubhashini ◽  
Vinaya Kumar R ◽  
Annapoorna Kini ◽  
Swetha H.B. ◽  
Srikanth Choudhary B.S. ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Diode Laser ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Toluidine Blue ◽  
Antimicrobial Effect ◽  
Positive Control ◽  
Negative Control ◽  
Root Canals

AIM: To evaluate antimicrobial effect of diode laser when used adjunctively with sodium hypochlorite, methylene blue or toluidine blue in Enterococcus faecalis inoculated root canals. METHODOLOGY: 70 extracted uniradicular premolars were prepared with ProTaper files and sterilized. 5 were kept aside as negative control (G1) and the remaining 65 were inoculated with Enterococcus faecalis and incubated for 7 days. Following this, 5 were kept as positive control (G2) and the other 60 were randomly distributed into six groups: G3, diode laser irradiation (1.5W) with sodium hypochlorite; G4, diode laser (3W) with sodium hypochlorite; G5, diode laser (1.5W) with methylene blue; G6, diode laser (3W) with methylene blue; G7, diode laser (1.5W) with toluidine blue; G8, diode laser (3W) with toluidine blue. Subsequently, turbidity was assessed and CFU count determined following intracanal sampling and plating. RESULTS: G4 exhibited significantly lower mean CFUs/mL (P˂0.001) among the experimental groups. G4 demonstrated the most (98.8%) while G7 exhibited the least antibacterial effect (28.66%). Highest turbidity was observed in G5 (0.1310) while the lowest was noted in G4 (0.0287). CONCLUSION: Diode laser used adjunctively with sodium hypochlorite exhibited superior antibacterial efficacy when compared to its use with photosensitizer dyes like methylene blue and toluidine blue.

Evaluation of topical therapies for the treatment of dermatophyte-infected hairs from dogs and cats

1995 ◽  
Vol 31 (3) ◽  
pp. 250-253 ◽  
Author(s):  
N White-Weithers ◽  
L Medleau
Keyword(s):  
Antifungal Activity ◽  
Sodium Hypochlorite ◽  
Povidone Iodine ◽  
Fungal Growth ◽  
In Vitro Study ◽  
Test Period ◽  
Microsporum Canis ◽  
Sodium Hypochlorite Solution ◽  
Hypochlorite Solution

Seven commonly used, topical antifungal products (i.e., lime sulfur, chlorhexidine, captan, povidone-iodine, sodium hypochlorite, and enilconazole solutions, and ketoconazole shampoo) were evaluated for their antifungal activity on Microsporum canis-infected hairs from dogs and cats in an in vitro study. Hairs were soaked or shampooed in each product for five minutes twice a week for four weeks. Of the seven products used in this study, lime sulfur and enilconazole solutions were superior in inhibiting fungal growth; no growth occurred on fungal cultures after two treatments with either product. Chlorhexidine and povidone iodine solutions were effective after four treatments, and sodium hypochlorite solution and ketoconazole shampoo inhibited fungal growth after eight treatments. Captan did not inhibit fungal growth during the test period.

Comparison of the Efficacy of a Hydrogen Peroxide Dry-Mist Disinfection System and Sodium Hypochlorite Solution for Eradication of Clostridium difficile Spores

2009 ◽  
Vol 30 (6) ◽  
pp. 507-514 ◽  
Author(s):  
F. Barbut ◽  
D. Menuet ◽  
M. Verachten ◽  
E. Girou
Keyword(s):  
Hydrogen Peroxide ◽  
Sodium Hypochlorite ◽  
Environmental Contamination ◽  
Bacterial Count ◽  
Sodium Hypochlorite Solution ◽  
Hypochlorite Solution ◽  
Colony Forming Units ◽  
In Situ Experiments

Objective.To compare a hydrogen peroxide dry-mist system and a 0.5% hypochlorite solution with respect to their ability to disinfect Clostridium difficile-contaminated surfaces in vitro and in situ.Design.Prospective, randomized, before-after trial.Setting.Two French hospitals affected by C. difficile.Intervention.In situ efficacy of disinfectants was assessed in rooms that had housed patients with C. difficile infection. A prospective study was performed at 2 hospitals that involved randomization of disinfection processes. When a patient with C. difficile infection was discharged, environmental contamination in the patient's room was evaluated before and after disinfection. Environmental surfaces were sampled for C. difficile by use of moistened swabs; swab samples were cultured on selective plates and in broth. Both disinfectants were tested in vitro with a spore-carrier test; in this test, 2 types of material, vinyl polychloride (representative of the room's floor) and laminate (representative of the room's furniture), were experimentally contaminated with spores from 3 C. difficile strains, including the epidemic clone ribotype 027-North American pulsed-field gel electrophoresis type 1.Results.There were 748 surface samples collected (360 from rooms treated with hydrogen peroxide and 388 from rooms treated with hypochlorite). Before disinfection, 46 (24%) of 194 samples obtained in the rooms randomized to hypochlorite treatment and 34 (19%) of 180 samples obtained in the rooms randomized to hydrogen peroxide treatment showed environmental contamination. After disinfection, 23 (12%) of 194 samples from hypochlorite-treated rooms and 4 (2%) of 180 samples from hydrogen peroxide treated rooms showed environmental contamination, a decrease in contamination of 50% after hypochlorite decontamination and 91% after hydrogen peroxide decontamination (P < .005). The in vitro activity of 0.5% hypochlorite was time dependent. The mean (±SD) reduction in initial log10 bacterial count was 4.32 ± 0.35 log10 colony-forming units after 10 minutes of exposure to hypochlorite and 4.18 ± 0.8 logl0 colony-forming units after 1 cycle of hydrogen peroxide decontamination.Conclusion.In situ experiments indicate that the hydrogen peroxide dry-mist disinfection system is significantly more effective than 0.5% sodium hypochlorite solution at eradicating С difficile spores and might represent a new alternative for disinfecting the rooms of patients with C. difficile infection.

scholarly journals In vitro study of the effect of Er: YAG laser irradiation on the apical sealing of different root canal sealers

2006 ◽  
Vol 14 (4) ◽  
pp. 260-263 ◽  
Author(s):  
Fernanda Vieira Medina ◽  
Manoel Damião Souza-Neto ◽  
Jacy Ribeiro Carvalho-Junior ◽  
Heid Sueli Leme dos Santos ◽  
Melissa Albanese Mezzena ◽  
...  
Keyword(s):  
Laser Irradiation ◽  
Sodium Hypochlorite ◽  
Root Canal ◽  
Er:Yag Laser ◽  
Sodium Hypochlorite Solution ◽  
Laser Application ◽  
Hypochlorite Solution ◽  
Root Canals ◽  
Group I ◽  
Significant Difference

OBJECTIVES: The aim of this study was to determine the effect of Er:YAG laser irradiation used to clean dentinal walls on the apical sealing of root canals filled with different types of sealers. Background Data: Laser application to the dentinal walls removed debris, rendering the root canals free of smear layers and leaving the dentinal canaliculi open. METHODS: Sixty-four maxillary canines obtained from laboratory files were instrumented with K-files (Dentsply, Maillefer, Ballaigues, Switzerland) using the crown-down technique, and irrigated with a 0.5% sodium hypochlorite solution. The specimens were divided into two groups of 32 teeth each. In group I, the teeth were instrumented and irrigated with sodium hypochlorite solution, and divided into four subgroups to be sealed with the different materials (Endofill, N-Rickert, Sealapex and Sealer 26). In group II, the root canals were subjected to Er:YAG laser irradiation (200 mJ, 7 Hz and 60 J total energy), followed by root canal sealing as in group I. RESULTS: The data showed lower levels of apical microleakage in the teeth filled with N-Rickert, Sealapex and Sealer 26 cements than in those sealed with Endofill (p < 0.01). No significant difference in microleakage was observed between teeth irrigated only with 0.5% sodium hypochlorite and those submitted to Er:YAG laser application (p > 0.05). CONCLUSIONS: The Er:YAG laser irradiation applied to the root canal walls was not able to prevent apical microleakage.

scholarly journals Effect of different rotary instrumentation techniques and thermoplastic filling on apical sealing

2004 ◽  
Vol 12 (1) ◽  
pp. 89-92 ◽  
Author(s):  
Mario Roberto Leonardo ◽  
Devanir de Araújo Cervi ◽  
Juliane Maria Guerreiro Tanomaru ◽  
Léa Assed Bezerra da Silva
Keyword(s):  
Methylene Blue ◽  
Sodium Hypochlorite ◽  
Root Canal ◽  
Root Canal Treatment ◽  
Sodium Hypochlorite Solution ◽  
Root Canal Filling ◽  
Hypochlorite Solution ◽  
Root Canals ◽  
Working Length ◽  
Ah Plus

The aim of this study was to evaluate apical sealing after root canal treatment using two different rotary instrumentation techniques and two thermoplastic root canal filling techniques. The study was performed in 115 human extracted mandibular premolars. After coronary access the apical foramen was opened with a # 15 K file 1 mm beyond the apex. Cleaning and shaping was subsequently carried out at the working length, 1 mm from the apex, with ProFile .04/.06 system (Dentsply/Maillefer), Quantec (Analytic Endodontics/Kerr) or by the step-back technique with 1% sodium hypochlorite solution as irrigating solution. The root canals were filled with Thermafil (Dentsply/Maillefer) or Microseal (Analytic Endodontics/Kerr) or by lateral condensation technique using AH Plus sealer (epoxy type). The teeth were immersed in 2% methylene blue under vacuum. Then, they were longitudinally sectioned. The results showed that the association of Profile and Thermafil Plus provide the best results (p<0.05). In conclusion, the association of different rotary instrumentation techniques and different filling systems influenced the apical sealing.

scholarly journals Antimicrobial Effect ofLippia sidoidesand Thymol onEnterococcus faecalisBiofilm of the Bacterium Isolated from Root Canals

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
H. N. H. Veras ◽  
F. F. G. Rodrigues ◽  
M. A. Botelho ◽  
I. R. A. Menezes ◽  
H. D. M. Coutinho ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Essential Oil ◽  
Statistical Difference ◽  
Antimicrobial Effect ◽  
Gas Chromatography Mass Spectrometry ◽  
Periapical Lesions ◽  
Popular Medicine ◽  
Root Canals ◽  
Mucosal Tissues

The speciesLippia sidoidesCham. (Verbenaceae) is utilized in popular medicine as a local antiseptic on the skin and mucosal tissues.Enterococcus faecalisis the bacterium isolated from root canals of teeth with persistent periapical lesions and has the ability to form biofilm, where it is responsible for the failure of endodontic treatments. Essential oil ofL. sidoides(EOLS) and its major component, thymol, were evaluated for reducing the CFU in biofilms ofE. faecalis in vitro. The essential oil was obtained by hydrodistillation and examined with respect to the chemical composition, by gas chromatography-mass spectrometry (GC-MS). The GC-MS analysis has led to the identification of thymol (84.9%) and p-cymene (5.33%). EOLS and thymol reduced CFU in biofilms ofE. faecalis in vitro(time of maturation, 72 h), with an exposure time of 30 and 60 min at concentrations of 2.5 and 10%. There was no statistical difference in effect between EOLS and thymol, demonstrating that this phenolic monoterpene was the possible compound responsible for the antimicrobial activity of EOLS. This study provides a basis for the possible utilization of EOLS as an adjuvant in the treatment of root canals that show colonization byE. faecalis.

scholarly journals Efficacy of continuous apical negative ultrasonic irrigation (CANUI) in penetration of simulated lateral canals in extracted teeth

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Pablo Castelo-Baz ◽  
Francisco J. Rodríguez Lozano ◽  
María J. Ginzo-Villamayor ◽  
Ramón Miguéns Vila ◽  
Juan Seoane-Romero ◽  
...  
Keyword(s):  
Sodium Hypochlorite ◽  
Positive Pressure ◽  
Sodium Hypochlorite Solution ◽  
Passive Ultrasonic Irrigation ◽  
Hypochlorite Solution ◽  
Root Canals ◽  
Working Length ◽  
Ultrasonic Irrigation ◽  
Curved Root Canals

AbstractThe aim of this study was to evaluate the efficacy of continuous apical negative ultrasonic irrigation into simulated lateral canals and the apical third in straight and curved root canals. Two simulated lateral canals were created 2, 4 and 6 mm from the working length in 120 single-rooted teeth (6 canals/tooth, n = 360 straight, n = 360 curved). The teeth were randomly divided into 3 experimental groups: positive pressure irrigation (PPI) (n = 20); passive ultrasonic irrigation (PUI) (n = 20); continuous apical negative ultrasonic irrigation (CANUI) (n = 20). 20% Chinese ink was added to a 5% sodium hypochlorite solution and delivered into the root canals. The results showed a significantly higher (P < 0.05) penetration of irrigant into the lateral canals and up to working length in the CANUI group for straight and curved roots. CANUI improves penetration into the lateral canals and up to the working length of the cleared teeth in straight and curved roots.

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