scholarly journals Evaluation of dentinal defects during root canal preparation using thermomechanically processed nickel-titanium files

2017 ◽  
Vol 11 (02) ◽  
pp. 157-161 ◽  
Author(s):  
Bertan Kesim ◽  
Burak Sagsen ◽  
Tugrul Aslan
Keyword(s):  
Root Canal ◽  
In Vitro Study ◽  
Nickel Titanium ◽  
Rotary Motion ◽  
Control Group ◽  
Continuous Rotation ◽  
Significant Difference ◽  
Group 5 ◽  
Group 2

ABSTRACT Objective: The aim of this study was to compare the incidence of root cracks after root canal instrumentation with thermomechanically processed nickel-titanium (Ni-Ti) files with different instrumentation kinematics. Materials and Methods: A total of 150 extracted mandibular premolars with mature apices and straight root canals were divided into five groups and used in this study. In Group 1, 30 teeth were prepared using hand K-files and assigned to control group, Group 2 was instrumented using K3XF Rotary files (SybronEndo, Glendora, CA, USA) with continuous rotary motion. The teeth in Group 3 were instrumented by ProTaper Next (Dentsply Maillefer, Ballaigues, Switzerland) rotary files which make asymmetric rotary motion, In Group 4, teeth were instrumented by RECIPROC (VDW, Munich, Germany) with reciprocation motion and in Group 5, teeth were instrumented by Twisted File (TF) Adaptive (SybronEndo, Orange, CA, USA) files that use combination of continuous rotation and reciprocation motion (n = 30/per group). All the roots were horizontally sectioned 3, 6, and 9 mm from the apex with a low speed saw under water cooling. Then, the slices were examined through a stereomicroscope to determine the presence of dentinal microcracks.Results: For the apical (3-mm) and coronal (9-mm) sections, the ProTaper Next and TF Adaptive produced significantly more cracks than the hand files, RECIPROC, and K3XF (P < 0.05). There was no significant difference between the experimental groups and control group at the 6-mm level (P > 0.05). Conclusions: Within the limitations of this in vitro study, all thermal-treated Ni-Ti instruments and hand files caused microcracks in root canal dentin.

scholarly journals Orange-Brown Precipitate: A Threat to the Success of Endodontic Treatment – An In Vitro Study

2020 ◽  
Vol 8 (D) ◽  
pp. 53-57
Author(s):  
Shashidhar Chandrashekhar ◽  
Dinesh Rao ◽  
Jyothi Shashidhar ◽  
Smitha Naik
Keyword(s):  
Root Canal ◽  
Isopropyl Alcohol ◽  
Sodium Thiosulfate ◽  
In Vitro Study ◽  
Control Group ◽  
Group 4 ◽  
Root Canal Irrigants ◽  
Group 5 ◽  
Brown Precipitate

AIM: The aim of the present study was to evaluate the effect of various intermediate irrigating solutions in the removal of orange-brown precipitate formed due to alternative use of sodium hypochlorite (NaOCl) and chlorhexidine (CHX) root canal irrigants. METHODS: The root canals of 50 mandibular premolars were prepared using ProTaper Universal Rotary instruments (Dentsply Maillefer, Ballaigues, Switzerland) up to size F3. The roots were randomly divided into five experimental groups specified by the intermediate irrigant used; Group 1: No intermediate irrigant used (control), Group 2: Saline, Group 3: 7% maleic acid (MA), Group 4: 4% sodium thiosulfate, and Group 5: 70% isopropyl alcohol (n = 10). After final irrigation with CHX, the specimens were evaluated for the presence/absence of orange-brown precipitate under stereomicroscope. RESULTS: Mean precipitate for all experimental groups was calculated using ANOVA F test which showed orange-brown precipitate (parachloroanaline) in all groups except Group 4 and Group 5 which were highly significant (p < 0.001) at all the levels of root canal. Intergroup pairwise comparison was done using Tukey’s post hoc test. CONCLUSION: About 7% MA is effective in minimizing the formation of orange-brown precipitate when used as intermediate irrigant between NaOCl and CHX. However, the precipitate was not observed with 4% sodium thiosulfate and 70% isopropyl alcohol.

scholarly journals Evaluation of the apical microleakage of MTA Fillapex, AH26, and Endofill sealers

2020 ◽  
Vol 23 (3) ◽  
Author(s):  
Saeedeh Galledar ◽  
Robab Farhang ◽  
Malek Abazari ◽  
Parisa Negahdar
Keyword(s):  
Root Canal ◽  
Test Group ◽  
In Vitro Study ◽  
Negative Control ◽  
Vitro Study ◽  
Control Group ◽  
Dye Penetration ◽  
Gutta Percha ◽  
Significant Difference

Objective: Proper apical seal plays an important role in the success of root canal treatment. The most common cause of failure of root canal therapy is known as the lack of adequate apical seal. The aim of this in vitro study was to compare the apical microleakage of MTA Fillapex, , and Endofill sealers using dye penetration method. Material and Methods: In this in vitro study, 72 single-rooted extracted human teeth were selected. The teeth were randomly divided into three experimental groups of 20 and two positive and negative control groups of 6. The canals were prepared by step-back technique and then filled with gutta-percha and one of the sealers mentioned. In the positive control group, the canals were filled with gutta-percha without sealer, and in the negative control group, the canals were prepared but not filled. The teeth were immersed in 2% methylene blue dye for 72 hours. The teeth were then cut longitudinally and the level of dye penetration was measured under a stereomicroscope. Data were analyzed by SPSS ver. 19 software, ANOVA and Bonferroni post-hoc tests. Results: The mean level of dye penetration in the Endofill test group was significantly higher than that in the  and MTA Fillapex test groups. While, the observed difference between  and MTA Fillapex groups was not statistically significant (p<0.05). Conclusion: The results of this study showed that  and MTA Fillapex sealers did not show any significant difference in apical seal properties. However, their sealing strength was significantly greater than Endofill sealer.KeywordsAH26 sealer; Endofill; MTA Fillapex; Microleakag

scholarly journals Pulp tissue dissolution capacity of QMix 2in1 irrigation solution

2015 ◽  
Vol 09 (03) ◽  
pp. 423-427 ◽  
Author(s):  
Dilara Arslan ◽  
Mehmet Burak Guneser ◽  
Alper Kustarci ◽  
Kursat Er ◽  
Seyda Herguner Siso
Keyword(s):  
Saline Solution ◽  
In Vitro Study ◽  
Control Group ◽  
Pulp Tissue ◽  
Tissue Samples ◽  
Canal Irrigation ◽  
Root Canal Irrigation ◽  
Significant Difference ◽  
Group 2

ABSTRACT Objective: The aim of this study was to evaluate the tissue dissolution efficacy of four root canal irrigation solutions (sodium hypochlorite [NaOCl], chlorhexidine gluconate [CHX], Octenidine [OCT], and QMix 2in1) on bovine pulp tissue. Materials and Methods: Fifty bovine pulp tissue samples, each weighing 6.55 mg, were prepared and randomly divided into four experimental groups and one control group (n = 10) according to the dissolution irrigants used: (1) 5.25% NaOCl group; (2) 2% CHX group; (3) OCT group; (4) QMix 2in1 group; and (5) control group (saline solution). These samples were then placed into special bovine dentin reservoir models and immersed for 1 h with each test solution (0.1 mL of each) at room temperature. The pulp samples were then blotted dry and weighed again. The percentage of weight loss was calculated. Statistically analyzed with one-way analysis of variance and post-hoc Tukey tests (P = 0.05). Results: Saline solution did not dissolve the bovine pulp tissue. All groups, except OCT, dissolved pulp samples more effectively than the control group (P < 0.05). The highest tissue dissolution was observed in 5.25% NaOCl group (P < 0.05). No statistically significant difference was found between the tissue-dissolving effect between QMix 2in1 and those of 2% CHX. Conclusions: Within the limitations of this in vitro study, NaOCl exhibited the best tissue-dissolving effect out of all solutions tested. CHX and QMix 2in1 were able to dissolve pulp tissue but less than NaOCl. OCT and saline solutions could not exhibit significantly tissue-dissolving effectiveness. This study shown that QMix 2in1 has little capacity to dissolve pulp tissue therefore used alone is not sufficient for this purpose.

scholarly journals Effectiveness of light-emitting diode exposure on photodynamic therapy against Enterococcus faecalis: in vitro study

2020 ◽  
Vol 53 (2) ◽  
pp. 71
Author(s):  
Nanik Zubaidah ◽  
Agus Subiwahjudi ◽  
Dinda Dewi Artini ◽  
Karina Erda Saninggar
Keyword(s):  
Photodynamic Therapy ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Pathogenic Bacteria ◽  
In Vitro Study ◽  
Control Group ◽  
Root Canal Irrigation ◽  
Group I ◽  
Significant Difference

Background: A successful root canal treatment eliminates pathogenic bacteria from infected root canals. The most common bacteria in root canal infections is Enterococcus faecalis (E. faecalis), due to its resistance to medicament and root canal irrigation. A photodynamic therapy (PDT) is a method of root canal disinfection that uses a combination of photosensitisers and light activation to eliminate bacteria in the root canal. The duration of the PDT irradiation results in the production of singlet oxygen and reactive oxygen species (ROS) to eliminate the E. faecalis bacteria. Purpose: To analyse the differences in the duration exposure of photodynamic therapy against the E. faecalis bacteria. Methods: The E. faecalis bacteria culture was divided into seven eppendorf tubes. Group I was a control group, and group II, III, IV, V, VI and VII were treated using PDT consisting of Toluidine Blue O (TBO) photosensitiser and light source irradiation for ten, 20, 30, 40, 50 and 60 seconds, respectively. After incubation, the number of bacteria was calculated by the Quebec Colony Counter and analysed using the Kruskal–Wallis test and the Mann–Whitney test (p <0.05). Results: There was a significant difference between the number of E. faecalis bacteria colonies in each treatment group (p <0.05). Group VI and VII, which had a longer exposure to PDT, showed a smaller amount of E. faecalis bacteria. Conclusion: The longer exposure of PDT results in a smaller amount of E. faecalis bacteria. The light irradiation of 50 seconds is the most effective to eliminate E. faecalis bacteria.

scholarly journals Evaluation of microcrack formation during root canal preparation using hand, rotary files and self-adjusting file in primary teeth: An in vitro study

2021 ◽  
Vol 15 (1) ◽  
pp. 35-41
Author(s):  
Anup Panda ◽  
Krishna Shah ◽  
Varsha Budakoti ◽  
Krishna Dere ◽  
Mira Virda ◽  
...  
Keyword(s):  
Root Canal ◽  
Primary Teeth ◽  
In Vitro Study ◽  
Permanent Teeth ◽  
Control Group ◽  
Root Canal Preparation ◽  
Root Dentin ◽  
Root Canal Morphology ◽  
Group 2

Background. Pediatric endodontics is a field with constant evolution, resulting in the shifting of paradigms from the use of conventional hand files to rotary files for biomechanical preparation in primary teeth. Biomechanical preparation plays a crucial role in the success of root canal treatment. Primary teeth need special attention since they differ from permanent teeth in root canal morphology. Cleaning and shaping of the canals damage the root dentin, leading to dentinal cracks. Newer techniques for root canal preparation, including Ni-Ti rotary files and SAF system, have been developed for use in pediatric endodontics to overcome the drawbacks of conventional methods. The present study compared dentinal defects formed by rotary systems in primary teeth. Methods. Eighty primary teeth were included. The teeth were decoronated with a diamond disc. All the roots were inspected for any pre-existing cracks or craze lines under transmitted light under a stereomicroscope. The specimens were then divided into four groups (n=20): group 1: control, group 2: hand files (HF), group 3: ProTaper files, and group IV: SAF files. The samples were instrumented according to the group they were assigned to. Results. The HF and SAF groups exhibited fewer microcracks. Dentinal microcracks were observed in roots prepared with rotary ProTaper files. There were significant differences between HF/SAF and rotary files (P<0.05). Conclusion. Stainless steel hand K-files and SAF instruments resulted in fewer dentinal damage than the ProTaper Universal files. SAF exhibited satisfactory results with minimal or no crack formation.

scholarly journals Effect of Adhesion Booster on Fluoride-releasing Composite: An in vitro Study

2018 ◽  
Vol 7 (2) ◽  
pp. 28-31
Author(s):  
Varunjeet Chaudhary ◽  
Sanad Singh Solanki ◽  
Varsha Yadav ◽  
Seema Lahoti
Keyword(s):  
Bond Strength ◽  
Shear Bond Strength ◽  
Testing Machine ◽  
In Vitro Study ◽  
Composite Resins ◽  
Control Group ◽  
Bonding Agents ◽  
Significant Difference ◽  
Group 2

Objective: To evaluate the shear bond strength of stainless steel brackets bonded with fluoride-releasing composite resins, comparing effect of adhesion booster and conventional primer.Materials & Method: Sixty extracted premolars were subjected to bracket bonding with fluoride-releasing composite resin; which were bonded by randomly divided into two groups of bonding agents: Group 1- conventional primer as control group, Group 2- adhesion booster. After bonding, the samples were thermocycled (500 cycles) at 5ºC and 55ºC temperatures. After 48 hours they were subjected to shear bond strength testing in occluso-gingival direction, using an MTS 810 Universal Testing Machine with load speed of 0.5 mm/min.Result: Mean shear bond strength was significantly more in samples bonded with adhesion booster (14.792±3.805 Mpa) as compared to conventional primers (11.327±4.047 Mpa). There was statistically significant difference in shear bond strength between the groups (p=0.001).Conclusion: The use of the adhesion booster significantly increased the bond strength of bracket bonded with fluoride-releasing composite.

scholarly journals Effect of photo-activated disinfection on bond strength of three different root canal sealers

2014 ◽  
Vol 08 (01) ◽  
pp. 085-089 ◽  
Author(s):  
Evren Ok ◽  
Huseyin Ertas ◽  
Gokhan Saygili ◽  
Tuba Gok
Keyword(s):  
Bond Strength ◽  
Root Canal ◽  
In Vitro Study ◽  
Significant Difference ◽  
Ah Plus ◽  
Group 3 ◽  
Group 5 ◽  
Root Canal Sealers ◽  
Push Out

ABSTRACT Introduction: The aim of this in vitro study was to evaluate the bond strength of Photo-Activated Disinfection (PAD) system to dentin with different root canal sealers by using a push-out test design. Materials and Methods: A total of 30 extracted mandibular premolar teeth with single and straight roots were used. The crowns were removed and the root canals were prepared by using ProTaper rotary files. The smear layer was removed and the roots were randomly divided into two groups (n = 15) according to the use of PAD system as the final disinfecting agent. Each group was then divided into 6 (n = 5) subgroups and obturated with gutta-percha and 3 different root canal sealers. The groups were Group 1: Sodium hypochlorite (NaOCl) + ethylenediaminetetraacetic acid (EDTA)-AH Plus sealer; Group 2: NaOCl + EDTA + PAD-AH Plus; Group 3: NaOCl + EDTA-Sealapex; Group 4: NaOCl + EDTA + PAD-Sealapex; Group 5: NaOCl + EDTA-mineral trioxide aggregate (MTA)-Fiallapex; and Group 6: NaOCl + EDTA + PAD-MTA-Fillapex. 1-mm thickness horizontal sections (n: 5 × 4 = 20) were sliced for the push-out bond strength measurement. Results: Group 3 and 4 showed significantly lower bond strengths compared with all the other groups (P < 0.05). No statistically significant difference was found among Groups 1, 2 and 5, but there was statistically significant difference between Group 5 and 6 (P < 0.05). Conclusion: This in vitro study indicated that the PAD system adversely affected the bond strength of the MTA Fillapex root canal sealer.

scholarly journals Effect of Artificial Saliva Contamination on Bond Strength to Pulp Chamber Dentin

2008 ◽  
Vol 02 (02) ◽  
pp. 086-090 ◽  
Author(s):  
Hale Ară ◽  
Nazmiye Dönmez ◽  
Sema Belli
Keyword(s):  
Bond Strength ◽  
Artificial Saliva ◽  
In Vitro Study ◽  
Adhesive System ◽  
Control Group ◽  
Pulp Chamber ◽  
Significant Difference ◽  
Group 2 ◽  
Saliva Contamination

ABSTRACTObjectives: The purpose of this in vitro study was to evaluate the effect of artificial saliva contamination on microtensile bond strength to pulp chamber dentin.Methods: Clearfil SE Bond (SEB) (Kuraray, JAPAN) adhesive system and Clearfil Photo Posterior (CPP) (Kuraray, JAPAN) composite resin were used. Twenty extracted caries-free human molar teeth were randomly distributed into four groups. Apart from a control group without contamination (Group 1), primed dentin surfaces were contaminated with artificial saliva (10 s), rinsed, dried, reprimed and bonded (Group 2), coated with adhesive, contaminated with artificial saliva, rinsed, dried, bonding procedures were repeated (Group 3), coated with adhesive, light cured, contaminated with saliva, rinsed, dried, treated with SE primer (SEP) and SEB (Group 4).After 24 hrs, the teeth were prepared for microtensile bond testing and tensile bond strength was measured (1mm/min). The data was calculated as MPa and analyzed using one-way ANOVA and Duncan test (P<.05).Results: The results indicated that Group 2 showed lowest bond strength when compared to the others (P<.05). No statistically significant difference was found between Groups 3 and 4 (P>.05).Conclusions: It was concluded that contamination during priming procedure has a negative effect on bond strength (P<.05). Although contamination of the uncured adhesive was not critical in this study (P>.05) any kind of contamination of the bonding area should, in principle, be avoided. (Eur J Dent 2008;2:86-90)

Comparative Study to Evaluate the Surface Detail Reproduction of Dental Stone after Immersion in Various Different Disinfectant Solutions, Under Stereomicroscope 10 X Magnification –An In-Vitro Study

2017 ◽  
Vol 9 (3) ◽  
Author(s):  
Rathika Rai ◽  
M. A. Easwaran ◽  
K. T. Dhivya
Keyword(s):  
Sodium Hypochlorite ◽  
In Vitro Study ◽  
Control Group ◽  
Immersion Method ◽  
Significant Difference ◽  
Surface Irregularities ◽  
Disinfectant Solution ◽  
Surface Detail ◽  
Group B

Aim: To evaluate the surface detail reproduction of dental stone this is immersed in different disinfectant solution and studied under stereomicroscope. Methodology: Total number of 30 specimens of dental stone (Type III) were made with measurements of 1.5cm diameter and 1cm height .This samples are divided in to 3 groups group A,B,C. were A is immersed in Distilled water which was taken as control group ;B is immersed in 2% Glutaraldehyde and C is immersed in 5%sodium hypochlorite. Each specimen were immersed in the disinfectant solution for 15 minutes and dried under room temperature for 24 hrs. After 24 hrs each specimens are studied under stereomicroscope for surface details. Result: The results showed no significant difference in the surface irregularities and porosities for a group 1 and group 2 except group 3 which showed significant increase in the porosities, surface irregularities and erosions after disinfection with 5% NaHOCl by immersion method. Conclusion: The surface detail reproduction capacity of die stone was adversely affected when 5% Sodium hypochlorite was used as disinfectant solution when compare d to control group and 2% Glutaraldehyde

scholarly journals In vitro study of human osteoblast proliferation and morphology on orthodontic mini-implants

2015 ◽  
Vol 85 (6) ◽  
pp. 920-926 ◽  
Author(s):  
Ricardo Carvalho Bueno ◽  
Roberta Tarkany Basting
Keyword(s):  
Chemical Composition ◽  
In Vitro Study ◽  
Chemical Components ◽  
Control Group ◽  
Osteoblast Proliferation ◽  
Mini Implants ◽  
Significant Difference ◽  
Implant Anchorage ◽  
Positive Effect

ABSTRACT Objective:  To evaluate the proliferation and morphology of human osteoblasts cultured on two brands of mini-implants after 24, 48, and 72 hours, in addition to the chemical composition found on their surface. Materials and Methods:  Two brands of mini-implant (Morelli and Neodent) were evaluated; polystyrene was used as a control group (n  =  3). Osteoblasts were cultured on the surface of sterilized mini-implants in a CO2 incubator at different time periods (24, 48, and 72 hours). Osteoblast proliferation was quantified by scanning electron microscopy using up to 5000× magnification, and cell morphology was analyzed by a single observer. For the chemical analysis, spectroscopy X-ray fluorescence was used to identify and quantify chemical components on the surface of the mini-implants. Results:  Two-way ANOVA showed no significant interaction between the factors studied (P  =  0.686). A Tukey test revealed no significant difference in osteoblast proliferation between the mini-implants at all studied periods; however, a difference in cell proliferation was detected between the Neodent and the control group (P  =  .025). For all groups, time had a direct and positive effect on osteoblast proliferation (P &lt; .001). The significant elements present in both brands of mini-implants were titanium, aluminum, vanadium, and iron. Conclusions:  Osteoblast proliferation was present on the mini-implants studied, which increased over time; however, no significant difference between brands was observed. No difference was seen between the mini-implants evaluated in terms of chemical composition. Cell adhesion after 72 hours suggests that areas of bone remodeling can be achieved, thus initiating the process of mini-implant anchorage.

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