Dietary amino acids affect intestinal Clostridium perfringens populations in broiler chickens

2005 ◽  
Vol 85 (2) ◽  
pp. 185-193 ◽  
Author(s):  
Darryl C Wilkie ◽  
Andrew G Van Kessel ◽  
Lisa J White ◽  
Bernard Laarveld ◽  
Murray D Drew

An experiment was performed to examine the effect of protein source and dietary amino acid profile on intestinal levels of C. perfringens in broiler chickens. Broiler chickens (age = 14 d; n = 192) were fed diets containing 400 g kg-1 crude protein with fish meal, meat/bone meal, feather meal, corn gluten meal, soy protein concentrate, pea protein concentrate, or potato protein concentrate as the primary protein source along with a control diet containing 230 g kg-1 crude protein. The birds were orally inoculated daily, with 1 mL (~1.0 × 108 CFU mL-1) of an overnight culture of C. perfringens between 14 and 21 d of age, killed at 28 d of age and C. perfringens numbers in ileum and cecum were enumerated. Birds fed fish meal, meat/bone meal, feather meal and potato protein concentrate had significantly higher intestinal C. perfringens counts than the birds fed corn gluten meal, soy or pea protein concentrates or the control diet (P < 0.05). The glycine content of the diets and ileal contents was significantly, positively correlated with C. perfringens numbers in ileum and cecum. Dietary glycine may be an important factor in the intestinal overgrowth of C. perfringens in broiler chickens. Key words: Clostridium perfringens, broiler chicken, amino acid, glycine, necrotic enteritis

2021 ◽  
Vol 24 (2) ◽  
pp. 116-123
Author(s):  
S. O. O. Oyewole ◽  
R. I. Salami

An experiment was conducted to evaluate the replacement value of poultry visceral offal meal (PVOM) substituting for fish meal (FM) on an equal protein basis in the diets of unsexed Ross-strain broiler chickens from day-old to 8 weeks of age. One hundred and fifty birds were allotted in groups of 10 into 15 equidimensional floor pens with floor space of 0.189m2 per bird. There were three replicates per treatment diet, with 10 birds per pen serving as a replicate. Five treatment diets, each for the starter and finisher phases of 5 and 3-week duration respectively were fed ad libitum. Diet A (control) contained 8% FM which was gradually replaced with PVOM at 2,3,4,6,6,90 and 9.20% in diets B, C, D and E respectively for both phases. All the diets were isocaloric (ca 12MJ   ME/kg) for both phases and isonitrogenous with 23 and 20% dietary crude protein contents for the starter and finisher diets respectively. Feed intake and feed conversion rations (FCR) were similar (P >0.05) on all diets in support of complete substitution. However , weight gain for 8 weeks on the control diet was better (P <0.05) than those on FM/PVOM- and PVOM-diets whose weight gains were similar (P < 0.05). The mean feed intake values of 4.031, 3.936, 3.760, 3.870 and 3.715 kg/bird were obtained for birds receiving diets A,B,C,D and E respectively while the equivalent FCRs were 2.326, 2.352, 2.233, 2.326 and 2.340. The mean weight gains for birds on diets A,B,C,D and E for 8weeks were 1.824, 1.688, 1.689, 1.676 and 1.590 kg/bird respectively. The dressed weight and weight of back, drumsticks, things and wings were similar (P >0.05) for all diets in favour of complete substitution of FM with PVOM. Mean dressing percentages were 58.24 to 69.07% . It is concluded that PVOM could replace FM completely in broiler diets.   


2019 ◽  
Vol 19 (1) ◽  
pp. 159-172 ◽  
Author(s):  
Tércia Cesária Reis de Souza ◽  
Araceli Aguilera Barreyro ◽  
Sara Rubio Rubio ◽  
Yanier Machado González ◽  
Konisgmar Escobar García ◽  
...  

AbstractTwo experiments were conducted to test if dehydrated porcine plasma (DPP) and potato protein concentrate (PPC) could be used as an alternative to antibiotics in starter diets for piglets. Experiment one was conducted to test if DPP and PPC in an antibiotic-free diet affected pig performance, and faecal consistency. Eighty-four piglets weaned at 22 days and weighing 6.9 kg were used. Piglets were fed for two weeks with one of four diets: a positive control diet with antibiotics (C+); and three other diets without antibiotics added with DPP, PPC, or DPP and PPC (DPP+PPC) to measure the average daily feed intake (ADFI), average daily gain (ADG), feed efficiency (FE), and incidence and severity of diarrhoea (ID and ISD respectively). In experiment two, twenty-four piglets weaned at 17 days and weighing 5.7 kg, were implanted at 21 days of age with a T-cannula at the terminal ileum to measure the apparent ileal digestibility (AID) and the apparent total tract digestibility (ATTD) of nutrients. Piglets were fed one of four diets: a positive control diet with antibiotics (C+); a negative control diet without antibiotics (C–), and two diets without antibiotics added with DPP, or PPC. The results of experiment one showed that the DPP diet was the most consumed diet during the first week, and the ADG and FE were similar among treatments. During the second week and the total experimental period the ADFI, ADG, and FE were similar among diets. The ID was lower in the C+ diet than other diets. The ISD was lower in the C+ diet than DPP and DPP+PPC diets; piglets fed PPC diet were similar to piglets fed C+ and DPP and DPP+PPC diets. The results of digestibility showed that crude protein AID was higher in piglets fed C+ and PPC diets than C– and DPP diets. Dry matter ATTD and energy ATTD were higher for piglets fed PPC than other diets. Further, crude protein ATTD of DPP and PPC diets tended to have a similar digestibility to that of C+ diet. The results suggest that PPC is a potential controller of post-weaning diarrhoea.


2011 ◽  
Vol 40 (5) ◽  
pp. 939-946 ◽  
Author(s):  
Felipe Barbosa Ribeiro ◽  
Eduardo Arruda Teixeira Lanna ◽  
Marcos Antonio Delmondes Bomfim ◽  
Juarez Lopes Donzele ◽  
Moisés Quadros ◽  
...  

It was determined in this work the coefficients of apparent and true digestibility of protein and amino acids of five feeds (corn, wheat bran, soybean meal, corn gluten meal, fish meal) in Nile tilapia. It was used 252 reverted Thai strain Nile tilapia (Oreochromis niloticus) in growth phase, with weight of 310 ± 9.68 g, distributed in experimental randomized blocks design, with five treatments, six replicates per treatment and seven fish per experimental unit. Each experimental diet contained a single source of protein, composed by the studied ingredients. An additional group of fish was fed protein-free diet for quantification of the endogenous fraction and determination of true digestibility coefficients. Digestibility was estimated by the indirect method by using chromium oxide at the concentration of 0.50% of the diet as a marker, performing fecal collection at every four hour interval by using decantation technique. Coefficients of apparent digestibility of the protein and amino acids are: corn, 83.57 and 82.45%; wheat bran 82.87 and 81.47%; soybean meal 91.12 and 89.41%; corn gluten meal 90.07 and 87.78%; fish meal 83.53 and 81.65% respectively. Coefficients of true digestibility of protein and the mean of the amino acids are: corn, 90.02 and 89.60%; wheat bran 89.62 and 89.14%; soybean meal 93.58 and 91.88%; corn gluten meal 92.50 and 90.34%; fish meal 86.01 and 84.27%, respectively.


2017 ◽  
Vol 41 (3) ◽  
pp. 243 ◽  
Author(s):  
Apriliana Devi Anggraini ◽  
Ferry Poernama ◽  
Chusnul Hanim ◽  
Nanung Danar Dono

This study was aimed to determine the effects of protease supplementation in diets with agricultural-livestock by products on the growth performance in broiler chickens. The commercial protease used in current study was produced by Bacillus licheniformis which based on keratinase. The treatment diets were a control basal diet without agricultural-livestock by-products and protease supplementation (P1); P1 + 0.05% protease; basal diet with meat bone meal (MBM) and distiller’s dried grain with soluble (DDGS) suppementation (P3); P3 + 0.05% protease (P4); basal diet with hydrolized chicken feather meal (HCFM) and DDGS supplementation (P5); P5 + 0.05% protease. The obtained data were statistically analyzed using Complete Randomized Design, and analyzed subsequently by Orthogonal Contrats Test. Results showed that protease supplementation in the diets containing agricultural-livestock by-products did not affect growth performance of broiler chickens in starter phase and overall age phase. However, in diets containing MBM, protease supplementation reduced the nett gain and feed conversion ratio (FCR) in finisher phase (P<0.05). In diets containing HCFM, protease supplementation increased nett gain and FCR (P<0.05), compared to those of containing MBM. It could be concluded that qualified HCFM, MBM, and DDGS can be used as alternatives for protein-energy source feed stuffs in starter phase, as they did not reduce growth performance of broiler chickens. Protease used in this study might be more effective in diets containing HCFM than those of containing MBM and DDGS.Keywords: Agricultural-livestock by-products, Broilers chickens, Growth performance, Protease.


2017 ◽  
Vol 43 (3) ◽  
pp. 457-465
Author(s):  
Luis Daniel Espinosa-Chaurand ◽  
Antonio Silva-Loera ◽  
Zaúl García-Esquivel ◽  
Lus Mercedes López-Acuña

In diets for Totoaba macdonaldi juveniles (26.3 ± 4.7g y 13.6 ± 1cm) the partial replacement of fishmeal protein (HP) with shrimp head meal (HCC) was evaluated, over their growth, survival, fed conversion (FCA) and chemical composition of tissues and the apparent digestibility coefficient of dry matter (CDA), protein (CDAP) and lipids (CDAL) of these diets. The HCC used were from the whole shrimp head sun dried (F) and smashed shrimp head dehydrated in a hot air drier. Diets were isoproteic (55.5% crude protein), isolipídic (15% lipids) and isocaloric (4.6 kcal g-1) replacing 0% (control diet; DC), 15% (F15 and M15) and 30% (F30 and M30) of the HP protein by the HCC. At 57th day, survival with HCC (99.44 ± 1.92%) was higher than DC (88.89 ± 3.85 %). The gain weight, weight specific growth (TCE) and total intake were not statistically different (P > 0.05) between organisms feed with HCC, however with the M30 diet the TCE had higher average (0.99 ± 0.06) and growth (19.82 ± 1.64 g/fish). With diet M30 the FCA was the best significantly (1.61 ± 0.13) and the higher CDA (66.18 ± 1.28), CDAP (86.51 ± 0.53) and CDAL (72.29 ± 1.10). It concluded that replaced protein of HP by HCC in diet for juvenile totoaba improved the growth and CDAs, yielding better results with the inclusion of macerated HCC with a replacement level of 30%.


1988 ◽  
Vol 51 (10) ◽  
pp. 807-810 ◽  
Author(s):  
DILEK HEPERKAN ◽  
ÍHSAN ALPERDEN

Level of mold contamination and mycoflora were determined for 144 mixed feed and feed ingredients, including corn, sunflower cake, soja cake, meat and bone meal, and fish meal. Four samples were found to be free of mold. Among the feed samples examined, the mold count has been found to be low (102 to 103 colonies/g) for fish meal, high (104 to 105 colonies/g) for meat-bone meal and sunflower cake, and extremely high (more than 105 colonies/g) for soja cake, corn and mixed feed. The predominant flora in the feed samples consisted of Penicillium, Aspergillus, Fusarium, Mucor and Eurotium, respectively. The most frequently encountered species was found to be Penicillium aurantiogriseum, followed by Aspergillus flavus.


2008 ◽  
Vol 43 (2) ◽  
pp. 271-274 ◽  
Author(s):  
Luís Gustavo Tavares Braga ◽  
Ricardo Borghesi ◽  
José Eurico Possebon Cyrino

The objective of this work was to determine the nutritional value of different protein sources for "dourado" (Salminus brasiliensis). Thirty juveniles per group (33.51±1.4 g) were hand fed on a reference diet (70%) added of tested ingredients (30%) and chromium oxide III (0.1%). Apparent digestibility coefficients of the gross energy (ADC GE), crude protein (ADC CP) and amino acids of the tested ingredients were evaluated. Corn gluten meal yielded the best results for ADC GE and ADC CP (95.7 and 96.9%, respectively) amongst plant ingredients. Spray-dried blood meal yielded the best values of ADC GE and ADC CP amongst animal ingredients (94.1 and 96.3%, respectively). Wheat bran yielded poorest ADCs coefficients (77 for ADC GE and 88.2% for ADC CP).


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