Effects of moist heat treatment on crude protein composition and degradability of field peas

1998 ◽  
Vol 78 (3) ◽  
pp. 453-456 ◽  
Author(s):  
A. F. Mustafa ◽  
D. A. Christensen ◽  
J. J. McKinnon
Keyword(s):  
Heat Treatment ◽  
Crude Protein ◽  
Protein Quality ◽  
Protein Composition ◽  
Steam Pressure ◽  
Heating Time ◽  
Neutral Detergent Fiber ◽  
Moist Heat ◽  
Field Peas

A study was conducted to determine the effects of moist heat treatment (autoclaving at 127 °C with a steam pressure of 117 kPa) of field peas for 10, 20 and 30 min on crude protein (CP) fractions, in situ ruminal CP degradability and post-ruminal availability of rumen undegraded protein. Heat treatment decreased (P < 0.05) buffer-soluble CP and increased (P < 0.05) the amount of CP associated with the neutral detergent fiber fraction without affecting that associated with acid detergent fiber. Ruminal CP degradability decreased (P < 0.05) while post-ruminal availability of rumen undegraded CP increased (P < 0.05) in cubic fashion as heating time increased. It was concluded that autoclaving of field peas for up to 30 min will reduce ruminal CP degradability without affecting protein quality of rumen undegraded protein. Key words: Pea, heat treatment, ruminal degradability, intestinal availability

Effect of heating on canola meal protein degradation in the rumen and digestion in the lower gastrointestinal tract of steers

1992 ◽  
Vol 72 (1) ◽  
pp. 83-88 ◽  
Author(s):  
S. A. Moshtaghi Nia ◽  
J. R. Ingalls
Keyword(s):  
Heat Treatment ◽  
Protein Digestibility ◽  
Steam Pressure ◽  
Heating Time ◽  
Canola Meal ◽  
Gi Tract ◽  
Moist Heat ◽  
Heat Treated ◽  
Meal Protein

Commercially available canola meal (CM) was moist heat treated at 127 °C with steam-pressure of 117 kPa for 15, 30, 45, 60 and 90 min. The effect of moist heat treatment of CM on rumen degradability and lower gastrointestinal (GI) tract digestibility of rumen escape dry matter (DM) and nitrogen (N) was determined using three rumen and two duodenal cannulated steers. Rumen degradability was estimated by incubating sample-filled small nylon bags in the rumen of steers for 10 and 16 h. Lower GI tract digestibility was estimated using a sequence of ruminal in situ incubation, in vitro incubation in an acid-pepsin solution and a mobile nylon bag technique. DM and N degradation of treated CM were significantly (P < 0.01) reduced in the rumen while the digestibility of DM and N in the lower GI tract was increased at all treatment times. At 16 h incubation, N disappearance declined in the rumen from 74.4 to 18.9% and increased in the lower GI tract from 16.2 to 64.2% for control and for CM heat treated for 45 min, respectively. Analysis of heat treated CM showed a significant reduction (P < 0.01) in the soluble N while the concentration of pepsin insoluble N, acid detergent insoluble N and neutral detergent insoluble N were increased as heating time increased. These increases in general were not associated with a decrease in N disappearance in the lower GI tract. Moist heat treatment was effective in increasing rumen escape of CM protein without adverse effect on protein digestibility in the lower GI tract. Key words: Canola meal, rumen degradability, post ruminal digestion, moist heat treatment, insoluble nitrogen

scholarly journals Evaluation of the Nutrient Composition, In Vitro Fermentation Characteristics, and In Situ Degradability of Amaranthus caudatus, Amaranthus cruentus, and Amaranthus hypochondriacus in Cattle

Animals ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 18
Author(s):  
Kim Margarette C. Nogoy ◽  
Jia Yu ◽  
Young Gyu Song ◽  
Shida Li ◽  
Jong-Wook Chung ◽  
...  
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Gas Production ◽  
Nutrient Composition ◽  
Neutral Detergent Fiber ◽  
Amaranthus Cruentus ◽  
Amaranthus Caudatus ◽  
Amaranthus Hypochondriacus

The amaranth plants showed high potential feed value as forage for ruminants. An in-depth study of this plant, particularly in cattle, will help extend its utilization as an alternative protein and fiber feed source in cattle feeding. In this study, the nutrient compositions of three different species of amaranth, Amaranthus caudatus L., Amaranthus cruentus L., and Amaranthus hypochondriacus L.—two varieties for each species, A.ca 74, A.ca 91, A.cu 62, A.cu 66, A. hy 30, and A. hy 48—were evaluated. The in vitro technique was used to evaluate the fermentation characteristics such as total gas production, total volatile fatty acids (VFA) concentration, pH, and ammonia concentration of the rumen fluid. Moreover, the effective degradabilities of dry matter (EDDM) and crude protein (EDCP) of the amaranth forages were determined through in situ bag technique. The amaranth forages: A. caudatus, A. cruentus, and A. hypochondriacus showed better nutritive value than the locally produced forages in Chungcheong province of Korea. The CP of the amaranth ranged from 11.95% to 14.19%, and the neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents ranged from 45.53% to 70.88% and 34.17% to 49.83%, respectively. Among the amaranth varieties, A. hypochondriacus 48 showed the most excellent ruminant feed nutrient quality (CP, 14.19%; NDF, 45.53%; and ADF, 34.17%). The effective degradabilities of dry matter (EDDM; 33–56%) and crude protein EDCP (27–59%) of the amaranth were lower compared to other studies, which could be due to the maturity stage at which the forages were harvested. Nonetheless, A. hypochondriacus 48 showed the highest EDDM (56.73%) and EDCP (59.09%). The different amaranth species did not differ greatly in terms of total VFA concentration or molar proportions, total gas production, or ammonia-N concentration. The high nutrient composition, and highly effective degradability of dry matter and crude protein, coupled with the favorable fermentation characteristics, suggest that the amaranth forages showed good to excellent feed quality for cattle.

scholarly journals SYNCHRONIZATION OF CARBOHYDRATE AND PROTEIN SUPPLY IN THE SUGARCANE BAGASSE BASED RATION ON IN SITU NUTRIENT DEGRADABILITY

2016 ◽  
Vol 41 (1) ◽  
pp. 28-36 ◽  
Author(s):  
F. F. Sani ◽  
L. K. Nuswantara ◽  
E. Pangestu ◽  
F. Wahyono ◽  
J. Achmadi
Keyword(s):  
Sugarcane Bagasse ◽  
Crude Protein ◽  
Degradation Kinetics ◽  
Nitrogen Supply ◽  
Neutral Detergent Fiber ◽  
Synchronization Index ◽  
In Situ Degradation ◽  
Protein Supply ◽  
Kinetics Of

Two adult male sheeps fitted with rumen cannula were used in two experiments to study the effects of synchronization of carbohydrate and nitrogen supply in sugarcane bagasse based total mixed ration (TMR) on in situ nutrient degradation. The first experiment was aimed to create three TMR with different synchronization index. Ingredient feedstuffs of TMR were evaluated for its in situ organic matter (OM) and nitrogen (N) degradation kinetics. On the basis of the OM and N degradation kinetics of feedstuffs, three sugarcane bagasse based TMR were formulated with synchronization indexes of 0.37; 0.50; and 0.63; respectively. The TMR had similar levels of crude protein (CP), total digestible nutrients, and neutral detergent fiber (NDF). In the second experiment, the three TMR with different synchronization index were evaluated for in situ degradability characteristics of OM, CP, NDF, and sulfur. The in situ degradation of OM in TMR were decreased (P<0.05) with the increasing of synchronization index. The higher synchronization index in TMR increased (P<0.05) CP degradation of CP. The NDF degradation decreased slightly by the alteration of synchronization index in TMR. The higher synchronization index in TMR reduced (P<0.05) in situ sulfur degradation, and this may not support to effects of synchronization of carbohydrate and nitrogen supply.  

scholarly journals Nutritive value of sugarcane silages with different bacterial additives and fermentation periods

2020 ◽  
Vol 21 ◽  
Author(s):  
Ivone Rodrigues da Silva ◽  
Francirose Shigaki ◽  
Rosane Cláudia Rodrigues ◽  
Ana Paula Ribeiro Jesus ◽  
Clésio dos Santos Costa ◽  
...  
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Nutritional Value ◽  
Nutritive Value ◽  
Soluble Fraction ◽  
B Value ◽  
Neutral Detergent Fiber ◽  
Acid Detergent Fiber ◽  
Fermentation Period

ABSTRACT The objective of this study was to evaluate the nutritive value of sugarcane silage with or without inoculation with P. acidipropionici or L. buchneri, over three fermentation periods. The experimental design was completely randomized in a 3 x 3 inoculant by fermentation period factorial arrangement (without inoculant, inoculant 1, inoculant 2; x three fermentation periods, 10, 60, 90 days). Values of pH, dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), hemicellulose (HEM) and lignin were determined and in situ DM degradability profiles were modelled for parameters a, b and c, potential degradation (A) and effective degradability (ED). The 90 day fermentation yielded a lower pH for both inoculants. There was an interaction between inoculant and fermentation period (P < 0.05) for DM content, with a reduction in silage DM without the additive at 90 days. The CP, HEM, ADF and lignin contents of sugarcane were not influenced by the treatments. The addition of P. acidipropionici provided the lowest NDF content at 10 days and presented a higher fraction a, potential degradation and ED. At 60 days, there was no variation in soluble fraction, the control silage showed a higher fraction b, higher potential degradation and ED. At 90 days of fermentation, L. buchneri silages presented a higher fraction a, degradation rate and DE and a higher b value was obtained in the silage without inoculant. Inoculants are effective in maintaining the silage DM content and nutritional value during prolonged fermentation periods.

Effect of heat treatment and heat treatment in combination with lignosulfonate on in situ rumen degradability of canola cake crude protein, lysine, and methionine

2020 ◽  
Vol 100 (1) ◽  
pp. 165-174 ◽  
Author(s):  
Piotr Micek ◽  
Katarzyna Słota ◽  
Paweł Górka
Keyword(s):  
Heat Treatment ◽  
High Temperature ◽  
Crude Protein ◽  
Dry Matter ◽  
Increased Temperature ◽  
Heat Treated ◽  
Cold Pressed ◽  
Temperature Heating ◽  
High Temperature Heating

The aim of this study was to determine the effect of heat treatment alone or in combination with the addition of lignosulfonate (LSO3) on canola cake protein, lysine, and methionine degradation in the rumen. Cold-pressed canola cake was left untreated, heated at 90, 110, 130, or 150 °C, or processed with 5% of LSO3 (in dry matter) and then heated. Effective rumen degradability of crude protein (CP), lysine, and methionine was less for treated than untreated canola cake (P < 0.05) and decreased with increased temperature of heating, but particularly when canola cake was heated at 150 °C (quadratic, P < 0.01). In general, effective rumen degradability of CP, lysine, and methionine was less for canola cake heated at 130 °C in combination with LSO3 compared with canola cake heat treated only (quadratic × LSO3 interaction, P ≤ 0.07). Results of this study indicate that high temperature heating (130 °C or greater for 60 min) may be necessary to protect canola cake protein from degradation in the rumen, and the combination of heat treatment and LSO3 may be more effective in protecting canola cake protein, lysine, and methionine from degradation in the rumen than the use of heat treatment only.

Effect of adding lignosulfonate and heat to canola screenings on ruminal and intestinal disappearance of dry matter and crude protein

2000 ◽  
Vol 80 (1) ◽  
pp. 215-219 ◽  
Author(s):  
Marina A. G. von Keyserlingk ◽  
E. Weurding ◽  
Mary Lou Swift ◽  
C. F. Wright ◽  
J. A. Shelford ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Gastrointestinal Tract ◽  
Key Words ◽  
Crude Protein ◽  
Dry Matter ◽  
Holstein Cows ◽  
Lower Gastrointestinal Tract ◽  
Rumen Degradation ◽  
Heat Treated

The effect of adding 5% calcium lignosulfonate (LSO3) and heat to canola screenings on in situ ruminal and intestinal dry matter (DM) and crude protein (CP) disappearance in Holstein cows was investigated. Treatments consisted of untreated canola screenings, canola screenings that had been heated to 100 °C for 30 or 60 min and canola screenings that had 5% (wt wt−1) LSO3 added and were subsequently heated at 100 °C for 0, 30 or 60 min. Results indicate that treatment of canola screenings with 5% LSO3 and heated to 100 °C for 60 min was effective in reducing rumen degradation of DM and CP with a corresponding increase in disappearance in the lower gastrointestinal tract when compared to untreated and heat-treated canola screenings. Key words: Canola screenings, rumen by-pass protein, heat treatment, lignosulfonate

Factors influencing composition and ruminal degradability of barley straw

1999 ◽  
Vol 79 (3) ◽  
pp. 343-351 ◽  
Author(s):  
G. W. Mathison ◽  
R. Soofi-Siawash ◽  
E. K. Okine ◽  
J. Helm ◽  
P. Juskiw
Keyword(s):  
Crude Protein ◽  
Nutritive Value ◽  
Neutral Detergent Fiber ◽  
Barley Straw ◽  
Medium Length ◽  
Key Words Barley ◽  
Ruminant Animals ◽  
Ruminal Degradability ◽  
Barley Genotypes

Chemical composition and in situ ruminal degradability characteristics for 25 and 40 genotypes of barley straw were measured for crops grown in 1994 and 1995, respectively. Straw from semidwarf genotypes contained more crude protein and less neutral detergent fiber (NDF) than that from genotypes with medium straw length and effective ruminal degradability was 9% higher (P < 0.01). Straw from two-row genotypes contained 19% more crude protein (4.4 vs. 3.7%) and 3% less NDF (75.2 vs. 77.6%) than that from six-row genotypes and effective ruminal degradability was 6% higher (P < 0.01) when genotypes with medium-length straw only were considered. Genotypes with smooth awns had higher rates of degradation but contained less of the slowly degraded fraction than straw with rough awns when straws of medium straw length were compared (P < 0.05). Straw from hulless genotypes contained more (P = 0.006) NDF than that from covered varieties. Both ambient and soil temperature were positively related (P < 0.05) to effective ruminal degradability of straw, possibly because of associated greater leafiness when temperatures were higher and there was more light. It was concluded that it is possible to select barley genotypes that have superior straw nutritive value for ruminant animals. Key words: Barley, straw, degradability, cattle, genotype, environment

Effect of extrusion on ruminal and intestinal disappearance of amino acids in white lupin seed

1992 ◽  
Vol 72 (1) ◽  
pp. 89-96 ◽  
Author(s):  
P. Cros ◽  
R. Moncoulon ◽  
C. Bayourthe ◽  
M. Vernay
Keyword(s):  
Amino Acids ◽  
Crude Protein ◽  
Protein Quality ◽  
Score Test ◽  
White Lupin ◽  
Lupin Seed ◽  
Chemical Score ◽  
Ruminal Degradation ◽  
Ruminal Digestion

The effect of extruding white lupin seed (WLS) at 120 or 150 °C on the degradability of crude protein in the rumen and the intestine of nonlactating Holstein cows was determined in situ. Nylon bags were incubated in the rumen for 16 h and then introduced into the small intestine, through a duodenal cannula, for subsequent recovery in feces. Extrusion of WLS at 120 or 150 °C decreased the degradability of crude protein in the rumen (86.9–73.6 vs. 98.4%) and increased the amount of crude protein disappearing in the intestine (11.8–25.0 vs. 0.8%). Extrusion did not alter the amino acid (AA) profile of WLS, although the AA composition of the WLS protein that escaped ruminal digestion differed markedly both quantitatively and qualitatively, from its initial composition. Extruding WLS increased intestinal disappearance of most of the AA, but variation in disappearance among AA was substantial. Thus, WLS proteins that are not degraded in the rumen differ in their potential as a source of absorbable AA in the intestine from the original source. Comparison of essential AA profiles of original sources to that of milk indicated that the sulphur AA were first-limiting in WLS, with valine, lysine and leucine being second through fourth limiting. The estimated AA chemical score (test-to-milk ratio) for original WLS was 64%. For the rumen undegraded protein fractions disappearing in the intestine, the limiting AAs in descending order were: sulphur AA, valine, phenylalanine plus tyrosine and threonine for raw WLS; sulphur AA, lysine, valine and histidine for WLS extruded at 120 °C and lysine, valine, histidine and sulphur AA for WLS treated at 150 °C. The corresponding AA chemical scores were: 63, 76 and 72%. Consequently, after extrusion, the ruminally undegraded protein of WLS that disappeared in the intestine showed a higher protein quality. Key words: Cow, lupin, seed, amino acids, extrusion, ruminal degradation, intestinal disappearance

scholarly journals Sugarcane Production Based on Mineral and Organic Nitrogen Fertilizers for Ruminant Feeding

2020 ◽  
Vol 8 (2) ◽  
pp. 678
Author(s):  
Elane Tyara de Jesus Siqueira ◽  
Rosane Claudia Rodrigues ◽  
José Roberto Brito Freitas ◽  
André Da Silva Alves ◽  
Zinaldo Firmino da Silva ◽  
...  
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Poultry Litter ◽  
Swine Manure ◽  
Neutral Detergent Fiber ◽  
Nitrogen Fertilizers ◽  
Acid Detergent Fiber ◽  
N Sources

Sugarcane may be a potential feed source for ruminants in tropical and subtropical areas. An important factor that can influence the nutritional quality of sugarcane for animal feeding is nitrogen fertilization management, however, studies on this subject are still scarce. In this context, the objective of this study was to evaluate the effect of nitrogen sources on the quality and productivity of sugarcane as a forage resource. The variety of sugarcane used was RB92579, with application of nitrogen (N) sources (urea, poultry litter, swine manure) and control (without N application). Stalk productivity and dry matter, crude protein, neutral detergent fiber, acid detergent fiber, lignin, Brix, NDF / Brix ratio, and in situ digestibility were analyzed during incubation periods of 6, 24, and 72 hours. At the end of the study, it was found that the N sources did not interfere with sugarcane stalk productivity (P> 0.05), with an average productivity of 102 t ha-1. The N treatments did not influence (P> 0.05) in the nutritional quality of sugarcane, when evaluating the contents of dry matter, crude protein, neutral detergent fiber, acid detergent fiber, lignin, Brix and NDF / Brix ratio. The dry matter degradation had an effect (P <0.05) for the different N sources and incubation times, where poultry litter and swine manure treatments showed higher values for degradation at 72 hours of incubation. The different N organic sources did not interfere in the chemical composition and productivity of the sugarcane culture, however it presented better results in the in situ degradability of the dry matter. This, it can be recommended that mineral nitrogen fertilizers can be replaced by organic fertilizers.

scholarly journals Analysis of the potentiality haying of native forage species in semiarid region

2017 ◽  
Vol 38 (5) ◽  
pp. 3319
Author(s):  
Enoque De Sousa Leão ◽  
Jacira Neves da Costa Torreão ◽  
Marcos Jácome de Araújo ◽  
Leilson Rocha Bezerra ◽  
Carlo Aldrovandi Torreão Marques ◽  
...  
Keyword(s):  
Crude Protein ◽  
Sun Exposure ◽  
Neutral Detergent Fiber ◽  
Mucuna Pruriens ◽  
Semiarid Region ◽  
Protein Loss ◽  
Forage Plants ◽  
Forage Species ◽  
Number Of Leaves

The objective of this study was to evaluate the potential hay production from Spiny Amaranth (Amaranthus spinosus), Hairy Woodrose (Merremia aegyptia), Malva (Sida galheirensis), Mucuna (Mucuna pruriens) and Ervanço (Froelichia humboldtiana), native forage species of the semiarid region of Brazil by observing morphological components of the plant, such as the dehydration curve, crude protein loss (CPL) curve, chemical composition of plant and hay and hay degradability in situ. There were differences (P < 0.05) among species on leaf, stem and inflorescence quantification with Ervanço, Hairy Woodrose, and Mucuna having a greater number of leaves. There was a linearly increasing response for the dehydration curve of the five forage plants species. Mucuna forage had the greatest hay point at 800 g kg-1 dried matter (DM) after 11.8 hours and Woodrose had a lower dehydration efficiency, which required 25 hours of sun exposure. There was no difference in CPL. Mucuna had the lowest crude protein (CP), neutral detergent fiber (NDF) and acid detergent fiber (ADF) content, 8.4, 67.7 and 73.8 g kg DM-1 in hay in relation to the plant, respectively. Ervanço, Spiny Amaranth, Hairy Woodrose and Malva hay had more of soluble fraction “a” of DM of 31.0, 26.2, 22.1 and 9.7 g kg-1 DM than Mucuna, respectively. Spiny Amaranth and Malva hay had values of 335.4 and 193.2 g kg-1 DM of fraction “b” more than Ervanço hay, respectively. For fraction “a’’ of CP, Spiny Amaranth and Hairy Woodrose hay obtained 312.6 and 227.4 g kg-1 CP more than that observed for Malva, respectively. Mucuna had better hay potential among the forage studied in the semiarid region of Brazil.

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