THE INFLUENCE OF DIETARY MONENSIN ON THE LH RESPONSE TO GnRH OR ESTRADIOL AND THE OVULATORY RESPONSE TO PMSG IN GILTS
To test the effects of monensin on endocrine responsiveness, 43 gilts of Yorkshire and Landrace breeding were individually fed a 16% crude protein basal diet (CT, n = 22) or the basal diet incorporating 33 mg kg−1 monensin (M, n = 21) for 25 d. Gilt weights and feed intakes were recorded at 7-d intervals for 21 d. Monensin did not significantly affect weight gain or feed intake but feed conversion was improved (P < 0.03). At 21 d, M (n = 6) and CT (n = 7) gilts were fitted with vena caval canulae and infused with 50 μg GnRH. Blood samples were obtained at 10-min intervals from 30 min before to 60 min after GnRH infusion. After a 48-h recovery period, these gilts received an injection of estradiol-17 B (E2) and further blood samples taken at the time of injection, at 24 and 36 h then at 6-h intervals until 78 h. All serum samples were assayed for LH and FSH concentrations. The LH response to GnRH was not affected by monensin, peak values being achieved in both groups 30 min after infusion. Serum FSH was not significantly influenced by GnRH in either group. Following E2 injection, the LH surge height was greater (P < 0.09) in monensin-fed gilts and a treatment by time interaction was evident (P < 0.001). Serum FSH was elevated in both groups but was unaffected by monensin. At 21 d, non-blood sampled gilts received an injection of 750 IU PMSG and were slaughtered 10 d later. An ovarian examination indicated that monensin-fed gilts tended (P < 0.1) to have a lower ovulation rate (13.2 ± 1.0 vs. 17.4 ± 1.9 for M and CT gilts, respectively). These data suggest that the feeding of monensin to gilts alters hypothalamic/pituitary responsiveness to stimulation and may enhance growth performance. Key words: Gilts, monensin, LH, FSH