SEED GERMINATION AND SEEDLING GROWTH CHARACTERISTICS OF ATRAZINE-SUSCEPTIBLE AND RESISTANT BIOTYPES OF Brassica campestris

1982 ◽  
Vol 62 (3) ◽  
pp. 733-739 ◽  
Author(s):  
L. R. MAPPLEBECK ◽  
V. SOUZA MACHADO ◽  
B. GRODZINSKI

Seed germination and seedling characteristics of atrazine-susceptible and resistant wild turnip rape biotypes (Brassica campestris L.) were examined. Seeds of the susceptible biotype germinated earlier, and the germinated seeds grew faster and emerged earlier than seeds of the resistant biotype. Seedlings of the susceptible biotype emerged from greater planting depths than the resistant seedlings. Emerged susceptible seedlings were larger, with longer hypocotyls and greater cotyledon area, and produced more dry matter than the resistant biotype seedlings. Differences in water content of germinated seeds of the two biotypes were noted, with the susceptible biotypes consistently showing a higher water content. There was no significant difference in the respiration rate of the two biotypes at the early stage of seedling growth.

1998 ◽  
Vol 78 (4) ◽  
pp. 611-614 ◽  
Author(s):  
Mingchu Zhang ◽  
M. Nyborg ◽  
E. D. Solberg

Seed-placed KCl often adversely affects seed germination and seedling growth because of the high salt index of the material. By coating KCl granules the adverse effect of the KCl can be reduced. Two greenhouse experiments with canola, barley and wheat, and one experiment in the field with barley were conducted to determine if normally adverse levels of seed-placed KCl fertilizer could be reduced when polymer-coated KCl is used. The results show that the number of germinated seeds and seedling dry matter were higher with the seed-placed coated KCl than the seed-placed regular non-coated KCl. Key words: KCl fertilizer, polymer-coated KCl, germination, seedling dry matter


2020 ◽  
Vol 41 (4) ◽  
pp. 1189
Author(s):  
Tatiane Sanches Jeromini ◽  
Thais Soares Pereira ◽  
Cibele Chalita Martins ◽  
Givanildo Zildo da Silva

Bauhinia scandens, an ornamental climbing vine, recently introduced in Brazil, in 2006 which has been gaining prominence due to its anti-tumor medicinal properties. A common feature amongst species of the Fabaceae family is dormancy due to the impermeability of the integument of the seed to water. The demand for seedlings of this species makes it necessary to elucidate the germination process and initial seedling growth. The objective of this study was to determine a method to overcome dormancy, thereby promoting seed germination and initial growth of B. scandens seedlings. The seeds were subjected to one of four treatments, besides the control: mechanical scarification, immersion in water (26 °C for 24 hours), immersion in hot water (86 °C for 24 hours, then 26 °C) and immersion in H2SO4 (95%) for 15, 30, 45, and 60 minutes. The seeds were evaluated for water content, germination (normal, abnormal seedlings, dormant and dead seeds), first count, mean germination time and relative frequency of germination. The seedlings were evaluated for emergence in sand, first count, emergence speed index, length and dry matter mass. B. scandens seeds should be immersed in H2SO4 for 45 and 60 minutes before sowing to overcome dormancy, in order to promote and standardize seed germination and enhance initial growth of the seedlings.


2021 ◽  
Vol 54 (1) ◽  
pp. 71-88
Author(s):  
Eric Bertrand Kouam ◽  
Toscani Ngompe-Deffo ◽  
Honoré Beyegue-Djonko ◽  
Marie Solange Mandou ◽  
Asafor Henry Chotangui ◽  
...  

Abstract Soil salinity represents a major constraint limiting crop production in arid and semi-arid countries. The effect of salinity induced by sodium chloride (NaCl) at five levels (0, 50, 100, 150 and 200 mM) was investigated on four germination traits and thirteen seedling growth characteristics in twenty cowpea [Vigna unguiculata (L.) Walp.] genotypes (ET11, KEB-CP004, KEB-CP006, KEB-CP009, KEB-CP 010, KEB-CP020, KEB-CP033, KEB-CP038, KEB-CP039, KEB-CP045, KEB-CP051, KEB-CP054, KEB-CP057, KEB-CP060, KEB-CP067, KEB-CP068, KEB-CP118, MTA22, NO74 and NO1036). The germination tests were carried out on Petri dishes in the laboratory while seedling growth experiments continued in plastic pots in the greenhouse, both setting up using a randomised complete block design with three replications. Genotypic responses were significant for all germination traits (p < 0.001). Germination percentage, germination rate index, and coefficient of velocity of germination were all decreased by salt stress. However, the mean germination time increased with increasing saline conditions. Significant differences were found between genotypes for most growth attributes. Growth rate (centimeter increased in height per week) decreased significantly with increasing salinity, starting at 100 mM NaCl (24.20% reduction, 2.66 cm / week) with maximum reduction (38.58%) corresponding to 2.16 cm/week observed at 200 mM NaCl, compared to control (3.51 cm/week growth rate). Also, significant decline in shoot weights, number of functional leaves and dry matter production were observed under salinity. Salinity also reduced water content in shoot and root and did not affect root weights. Under salinity, significant correlations were found between all germination variables (p < 0.001). Growth rate was significantly associated with ten out of the twelve other seedling growth traits. Also, the dry matter production under salinity was significantly associated with all other seedling growth characteristics with the exception of root water content. Given the effect of salt stress, cowpea genotypes, namely NO1036, KEB-CP004, KEB-CP038 and KEB-CP051, were the most tolerant while KEB-CP068 and ET11 were the most sensitive ones. The results confirm substantial genetic variation in salt stress tolerance among the studied genotypes. The most tolerant genotypes should be further explored in genetic improvement programs and should be promoted for culture in regions affected by salinity.


2021 ◽  
Vol 25 (8) ◽  
pp. 1547-1550
Author(s):  
O.A. Majekodunmi ◽  
I.O. Abiola ◽  
A.M. Aderemi ◽  
J.O. Adedipe ◽  
O.G. Ogunwale ◽  
...  

This study investigated the effect of sowing media on the seed germination and subsequent seedling growth of Senna fistula L. Treatments were T1 (Top soil), T2 (River sand), T3 (Grinded coconut husk) and T4 (weathered sawdust), replicated two times for seed germination. The treatments were arranged in a Completely Randomized Design (CRD) and the data generated was subjected to analysis of variance (ANOVA) at 5% level of significance, while mean separation where appropriate was done using Least Significant Difference (LSD). The different sowing media used had positive effect on the seed emergence, the seeds planted in river sand had the highest emergence percentage of 70%. The different sowing media used did not have any significant effect on the vegetative growth parameters, statistically, they had same effect on the parameters measured. Nonetheless, seedlings from river sand produced highest number of leave (20.4), seedlings from topsoil produced highest plant height and largest leaf area of l0.62 cm and 37.l4 cm2 respectively. The seedlings from weathered sawdust produced the highest stem diameter of 0.79 mm, while seedlings from grinded coconut husk had lowest values for all the growth parameters measured. Therefore, it is recommended that river sand or weathered sawdust can be used to propagate Senna fistula seeds.


2020 ◽  
Vol 48 (2) ◽  
pp. 938-953
Author(s):  
Taieb TOUNEKTI ◽  
Mosbah MAHDHI ◽  
Zarraq AL-FAIFI ◽  
Habib KHEMIRA

Seeds of three sorghum (Sorghum bicolor (L.) Moench.) varieties from Southwest Saudi Arabia were used to investigate the potential of osmopriming with polyethylene glycol (PEG 8000) to improve germination performance, seed reserve utilization and early seedling growth and drought stress tolerance. The primed (PS) and unprimed (UPS) seeds of the three sorghum varieties were germinated for 8 days under increasing PEG-induced osmotic stress. The treatments were arranged in a completely randomized design, in a factorial arrangement, with three sorghum cultivars (‘Zaydia’, ‘Shahbi’ and ‘Ahmar’) and four osmotic potentials (0.0; -0.4; -0.8 and -1.2 MPa) with four replicates of 50 seeds each. The results showed that drought stress affected seed germination and seedling emergence and establishment, but increased the activity of the antioxidant enzyme catalase (CAT). The strongest inhibition of germination and growth occurred at the highest PEG concentration and a significant difference was noticeable between the studied varieties. We confirmed also that seed osmopriming improved seed germination performance, seedling growth and enhanced the CAT activities while reduced malonyldialdehyde (MDA) accumulation and electrolyte leakage (EL) in the drought-stressed seedlings. Seed priming have enhanced also the α-amylase and total proteases activities in all varieties. The largest increase of these hydrolysing enzymes was shown in ‘Ahmar’. Furthermore, the PEG priming lead to improvement of the weight of utilized (mobilized) seed reserve (WUSR), seed reserve depletion percentage (SRDP) and total seedling dry weight (SLDW) of sorghum seedlings under water stress conditions. Still, the highest values or all three parameters were found in the ‘Ahmar’ variety. Under increasing drought stress conditions, ‘Ahmar’ showed the highest yield stability index (YSI) and the least EL and MDA contents in comparison to the other two varieties during the seedling establishment stage. Therefore, the former variety can tolerate better a rigorous water stress condition. ‘Zaydia’ appears to be the most vulnerable to drought stress. Thus, the use of species or varieties with eminent seed metabolic quality is an advantageous trait in drought-prone regions.


FLORESTA ◽  
2015 ◽  
Vol 45 (4) ◽  
pp. 845 ◽  
Author(s):  
Letícia Ramon De Medeiros ◽  
Manoela Andrade Monteiro ◽  
Patrícia Migliorini ◽  
Marilia Lazarotto ◽  
Lilian De Tunes

AbstractThe objective of this study was to evaluate the germination of Australian cedar seeds (Toona ciliata M. Roem) in three different substrates and two photoperiods and evaluate the effect of salt stress with sodium chloride (NaCl) on the viability and vigor of seeds, conducting two experiments. The first experiment was conducted in a constant temperature room at 25 C and two light regimes 12h 24h white light; and three substrates: sand, between paper and on paper. Evaluations were performed on the seventhand twenty-first day after sowing. Results were expressed as percentage of Normal Seedlings, Abnormal Seedlings and Dead Seeds, Number of True Leaves, Fresh, Dry Matter, Seedling Length and Root. The second experiment used constant 25 C and photoperiod of 12 hours light. With treatments T1 (0mM: distilled water); 25mMNaCl T2; T3 50mMNaCl; T4 and T5 100mMNaCl 75mMNaCl. We evaluated PCG, G, IVG, CP, CR. The photoperiod of 24h light, does not influence the final seed germination and the use of paper on substrate provided the highest percentage of germinated seeds. As to the effect of salt stress, the concentration of 50mMsalt, caused damage to the development of seedlings.ResumoPadronização do teste de germinação e resposta ao estresse salino por NaCl em sementes de Toona ciliata. O objetivo do trabalho foi avaliar a germinação de sementes de cedro australiano (Toona ciliata M. Roem) em três substratos com dois fotoperíodos e avaliar o efeito do estresse salino com cloreto de sódio (NaCl) na viabilidade e vigor de sementes. O experimento I foi conduzido em ambiente com temperatura constante a 25 ºC e dois regimes de luminosidade, 12h de 24h de luz branca; três substratos: areia, entre papel e sobre papel. As avaliações foram realizadas no sétimo e vigésimo primeiro dia após a semeadura. Os resultados expressos em porcentagem de Plântulas Normais, Plântulas Anormais e Sementes Mortas, número de Folhas Verdadeiras, Massa Fresca, Massa Seca, Comprimento de Plântula e Raiz. O experimento II utilizou temperatura constante a 25 ºC e fotoperíodo de 12 horas luz. Com os tratamentos T1 (0 mM: água destilada); T2 25 mMNaCl; T3 50 mMNaCl; T4 75 mMNaCl e T5 100 mMNaCl. Avaliando-se PCG, G, IVG, CP e CR. O fotoperíodo de 24h de luz, não influencia na germinação e o uso do substrato sobre papel proporcionou a maior germinação. Quanto ao efeito do estresse salino, a concentração de 20 mM de sal, prejudicou o desenvolvimento das plântulas.Palavras-chave: Sementes florestais; análise de sementes, vigor, salinidade.


HortScience ◽  
2011 ◽  
Vol 46 (4) ◽  
pp. 651-654 ◽  
Author(s):  
Qi Zhang ◽  
Sheng Wang ◽  
Kevin Rue

Salinity tolerance of 12 turfgrasses in four groups, creeping bentgrass (Agrostis stolonifera L.), fescues (Festuca spp.), kentucky bluegrass (Poa pratesis L.), and alkaligrass [Puccinellia distans (Jacq.) Parl.], was evaluated using three germination methods. Seeds were germinated on 1% agar medium, on germination paper, or in a hydroponic system under salinity levels of 0, 5, 10, 15, or 20 g·L−1 NaCl. Germination rate and seedling growth of each grass were determined. Salinity reduced the final germination rate (FGR), daily germination rate (DGR), and seedling leaf area (LA) in all tests. On agar medium, no significant difference in salinity tolerance was observed among the four turf groups; however, ‘Turf Blue’ kentucky bluegrass with a corn starch-based coating (coated ‘Turf Blue’) showed a significant higher salinity tolerance than the uncoated one. Using germination paper, creeping bentgrass required the highest salinity level to cause 50% reduction in FGR followed by alkaligrass, fescues, and kentucky bluegrass. Kentucky bluegrass required the lowest salinity level (9.5 g·L−1) to reduce DGR by 50%. With the hydroponic system, alkaligrass required a salinity level of 26.3 g·L−1 to reduce FGR by 50%, the highest among the four groups. Alkaligrass showed again the highest salinity tolerance with an average of 12.7 g·L−1 needed to reduce LA by 50%. Among the grasses, coated ‘Turf Blue’ kentucky bluegrass, ‘Declaration’ creeping bentgrass, and ‘Fults’ alkaligrass showed the highest salinity tolerance when evaluated on agar medium, on germination paper, or in the hydroponic system, respectively. The present study determined the salinity tolerance of 12 turfgrasses at seed germination and early seedling growth stages and showed that the germination method was a factor affecting the evaluation result and it should be considered in a seed germination test of turfgrass for salinity tolerance.


2019 ◽  
Vol 17 (2) ◽  
pp. 153-160 ◽  
Author(s):  
Md. Arifur Rahman ◽  
Md. Sohanur Rahman ◽  
K.M. Mohiuddin ◽  
Md. Akhter Hossain Chowdhury ◽  
Md. Abul Khair Chowdhury

Indiscriminate discharge of industrial effluent has become a serious problem for the agro-ecological environment in most of the areas of Bangladesh. The effects of loom-dye effluents on seed germination and early seedling growth of rice (Oryza sativa L.) were investigated by conducting an experiment in the laboratory of the Department of Agricultural Chemistry of Bangladesh Agricultural University, Mymensingh. Three types of loom-dye effluents were applied in sterilized petridishes at different loading ratios. Seven treatments (i.e., T0, T1, T2, T3, T4, T5 and T6 with 0, 5, 10, 25, 50, 75 and 100% effluent, respectively) of each effluent were used following completely randomized design (CRD) and replicated three times. Subsequently, Pb, Fe, Zn, Mn and Cr accumulation were also investigated in the harvested rice seedlings. Rice seed showed a significant difference in germination percentage with varying levels of effluent application at different days after setting of germination. The decreased seed germination rate and seedling growth of rice were observed with increased concentration of loom-dye effluents. The highest germination speed (97.8%) was obtained from control whereas the lowest germination speeds were obtained from T5 of black, and T6 of both black and violet effluents. Phytotoxic effects of loom-dye effluents on germination and radical length were extreme at 100% effluent concentration having the order of black > violet > pink. The maximum radical length (6.4 cm) and plumule length (7.5 cm) were observed with T1 of pink dye effluent whereas the minimum length of radical and plumule were obtained from T6 treatment of the effluents. The highest fresh weight (39.8 mg petridish–1), dry weight (5.7 mg petridish–1) and seedling vigor index (746.7%) were also observed from T1 of pink dye effluent. The maximum uptake of Pb, Fe, Mn and Zn was 0.48, 3.81, 0.79 and 0.13 μg g−1, respectively. The uptake of Cr was below the detectable limit. Total heavy metal accumulation in rice was in the following order: Fe>Mn>Pb>Zn>Cr. Results showed that the higher concentration of loom-dye effluent showed the higher toxic effects on different parameters of germination and early seedling growth compared with the lower effluent concentrations. J. Bangladesh Agril. Univ. 17(2): 153–160, June 2019


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