scholarly journals STANDARDIZATION OF GERMINATION TEST AND RESPONSE TO NACL SALT STRESS IN Toona cliliata SEEDS

FLORESTA ◽  
2015 ◽  
Vol 45 (4) ◽  
pp. 845 ◽  
Author(s):  
Letícia Ramon De Medeiros ◽  
Manoela Andrade Monteiro ◽  
Patrícia Migliorini ◽  
Marilia Lazarotto ◽  
Lilian De Tunes
Keyword(s):  
Salt Stress ◽  
Sodium Chloride ◽  
Seed Germination ◽  
Dry Matter ◽  
Distilled Water ◽  
Germination Test ◽  
Light Regimes ◽  
Seedling Length ◽  
Toona Ciliata ◽  
Germinated Seeds

AbstractThe objective of this study was to evaluate the germination of Australian cedar seeds (Toona ciliata M. Roem) in three different substrates and two photoperiods and evaluate the effect of salt stress with sodium chloride (NaCl) on the viability and vigor of seeds, conducting two experiments. The first experiment was conducted in a constant temperature room at 25 C and two light regimes 12h 24h white light; and three substrates: sand, between paper and on paper. Evaluations were performed on the seventhand twenty-first day after sowing. Results were expressed as percentage of Normal Seedlings, Abnormal Seedlings and Dead Seeds, Number of True Leaves, Fresh, Dry Matter, Seedling Length and Root. The second experiment used constant 25 C and photoperiod of 12 hours light. With treatments T1 (0mM: distilled water); 25mMNaCl T2; T3 50mMNaCl; T4 and T5 100mMNaCl 75mMNaCl. We evaluated PCG, G, IVG, CP, CR. The photoperiod of 24h light, does not influence the final seed germination and the use of paper on substrate provided the highest percentage of germinated seeds. As to the effect of salt stress, the concentration of 50mMsalt, caused damage to the development of seedlings.ResumoPadronização do teste de germinação e resposta ao estresse salino por NaCl em sementes de Toona ciliata. O objetivo do trabalho foi avaliar a germinação de sementes de cedro australiano (Toona ciliata M. Roem) em três substratos com dois fotoperíodos e avaliar o efeito do estresse salino com cloreto de sódio (NaCl) na viabilidade e vigor de sementes. O experimento I foi conduzido em ambiente com temperatura constante a 25 ºC e dois regimes de luminosidade, 12h de 24h de luz branca; três substratos: areia, entre papel e sobre papel. As avaliações foram realizadas no sétimo e vigésimo primeiro dia após a semeadura. Os resultados expressos em porcentagem de Plântulas Normais, Plântulas Anormais e Sementes Mortas, número de Folhas Verdadeiras, Massa Fresca, Massa Seca, Comprimento de Plântula e Raiz. O experimento II utilizou temperatura constante a 25 ºC e fotoperíodo de 12 horas luz. Com os tratamentos T1 (0 mM: água destilada); T2 25 mMNaCl; T3 50 mMNaCl; T4 75 mMNaCl e T5 100 mMNaCl. Avaliando-se PCG, G, IVG, CP e CR. O fotoperíodo de 24h de luz, não influencia na germinação e o uso do substrato sobre papel proporcionou a maior germinação. Quanto ao efeito do estresse salino, a concentração de 20 mM de sal, prejudicou o desenvolvimento das plântulas.Palavras-chave: Sementes florestais; análise de sementes, vigor, salinidade.

scholarly journals Effects of Sodium Chloride and Sodium Sulfate on Haloxylon ammodendron Seed Germination

2020 ◽  
Vol 12 (12) ◽  
pp. 4927
Author(s):  
Zhazira Zhumabekova ◽  
Xinwen Xu ◽  
Yongdong Wang ◽  
Chunwu Song ◽  
Alzhan Kurmangozhinov ◽  
...  
Keyword(s):  
Salt Stress ◽  
Seed Germination ◽  
High Salinity ◽  
Seed Viability ◽  
Gobi Desert ◽  
Distilled Water ◽  
Haloxylon Ammodendron ◽  
High Recovery ◽  
Desert Areas ◽  
Germinated Seeds

Haloxylon ammodendron is a perennial xerophyte that can survive in extremely harsh desert conditions of Central Asia. This study evaluated the effect of salinity, and their ability to recover on seed germination of H. ammodendron, which were collected at three different desert areas, Bakanas takyr plain (H1), Gurbantüngüt Desert (H2), and Gobi Desert (H3), respectively. Seeds were treated with different concentrations of NaCl and Na2SO4 (0.00 (control), 0.05, 0.10, 0.20, 0.40, 0.60, 0.80, 1.00, 1.20, and 1.40 mol/L) to detemine the germination and recover ability to salt stress. The results of the study were that H. ammodendron were more resistant to Na2SO4 than to NaCl. Regarding inhibition of seed germination H. ammodendron was in the following order: H3 > H2 > H1. Based on the tolerance and recovery, seeds can be demonstrated as follows: NaCl: H3 > H1 > H2; Na2SO4: H1 = H2 = H3. Non-germinated seeds in all salt treatments with low osmotic potential showed high recovery when transferred to distilled water, indicating that these treatments were not too toxic to affect seed viability. These results suggest that H. ammodendron can withstand high levels of salinity at three desert environments. Thus, H. ammodendron could be used to reconstruct vegetation and sustanbility development in the desert areas with high salinity.

scholarly journals EFFECTS OF 24-EPIBRASSINOLIDE ON GERMINATION AND GROWTH OF TOMATO SEEDLINGS UNDER SALT STRESS

2021 ◽  
Vol 8 (1) ◽  
pp. e4842
Author(s):  
Sebastião De Oliveira Maia Júnior ◽  
Jailma Ribeiro de Andrade ◽  
Robson Felipe de Lima ◽  
Rafaela Félix Basílio Guimarães ◽  
Allesson Ramos de Souza ◽  
...  
Keyword(s):  
Salt Stress ◽  
Seed Germination ◽  
Dry Matter ◽  
Growth Traits ◽  
Tomato Cultivar ◽  
Tomato Seedlings ◽  
Tomato Seeds ◽  
Seedling Length ◽  
Germination Speed ◽  
Germination And Growth

Salinity is one of the environmental stresses that most affects plants' vital processes, especially germination. Brassinosteroids, including 24-epibrassinolide (EBL), have multiple actions in essential processes in plants. Thus, this research's objective was to evaluate the effects of EBL on tomato seeds' physiological conditioning on germination, growth, and production of dry seedling mass under salinity conditions. The experiment was carried out in a 2x5 factorial scheme, as follows: two concentrations of EBL (0 and 10-6 M) and five levels of salinity (0.5 control; 1.5; 3.0; 4.5 and 6.0 dS m-1), consisting of ten treatments, with four replications of 50 seeds. In isolation, the salt stress reduced the percentage of germination and germination speed index from four to 14 days, besides all the plants' growth traits. In turn, the EBL increased the percentage of germination, germination speed index, seedling length, and radicle dry matter. The application of 10-6 M of EBL in seed immersion no effect on the seed germination of IPA 6 tomato cultivar under salt stress but increases the root length and the dry matter of the seedlings.

scholarly journals Imbibition, Germination, and Early Seedling Growth Responses of Light Purple and Yellow Seeds of Red Clover to Distilled Water, Sodium Chloride, and Nutrient Solution

Sci ◽  
2019 ◽  
Vol 1 (2) ◽  
pp. 51 ◽  
Author(s):  
Ana Costa ◽  
Luís Dias ◽  
Alexandra Dias
Keyword(s):  
Sodium Chloride ◽  
Seed Germination ◽  
Nutrient Solution ◽  
Seedling Growth ◽  
Red Clover ◽  
Superior Performance ◽  
Distilled Water ◽  
Seed Imbibition ◽  
Early Seedling Growth ◽  
Early Seedling

The seeds of red clover are heteromorphic and two color morphs can be visually recognized, light purple and yellow, resulting from heterozygosity and recessive homozygosity at two loci. Here, we report the responses of seed imbibition, seed germination, and early seedling growth of the two morphs to distilled water, sodium chloride, and complete nutrient solution. The sensitivityof red clover seeds to treatments increased with the stage of development in what seems to be a cumulative process. No differences were found in seed imbibition between morphs or between treatments. In seedling growth, on the contrary, treatments were always effective, but differences between morphs were only observed in seeds that were treated with nutrient solution, whereas in the intermediate stage of seed germination, the effects by treatments were observed together with the appearance of differences between morphs in distilled water and in the treatment by sodium chloride solution. Simultaneously, the superior performance of the yellow morph that was found in germination, which appears to be a trait stable across cultivars of red clover seeds, turned into a superior performance of the light purple morph in seedling growth.

scholarly journals Effects of light, temperature, salinity, and maternal habitat on seed germination of Aeluropus lagopoides (Poaceae): an economically important halophyte of arid Arabian deserts

Botany ◽  
2020 ◽  
Vol 98 (2) ◽  
pp. 117-125 ◽  
Author(s):  
Arvind Bhatt ◽  
Sanjay Gairola ◽  
María Mercedes Carón ◽  
Andrea Santo ◽  
Valentina Murru ◽  
...  
Keyword(s):  
Control Group ◽  
Distilled Water ◽  
Light Regimes ◽  
Aeluropus Lagopoides ◽  
Percent Germination ◽  
Recovery Percentage ◽  
Desert Habitat ◽  
Arid Desert ◽  
Saline Solutions ◽  
Germinated Seeds

In this study, salt tolerance during germination of Aeluropus lagopoides (L.) Trin. was tested using fresh seeds collected from three different maternal habitats under three thermoperiods and two light regimes. Additionally, we tested the ability of non-germinated seeds that had been exposed to different concentrations of NaCl to recover their germination in distilled water. The results showed a significant effect of seed source, temperature, and salinity, and some of their two- and three-way interactions on final germination and recovery percentage. The seeds from non-saline provenances had the highest percentages for germination (ca. 79%) under the 35/25 °C temperature regime, whereas the lowest percentages for germination (ca. 21%) was recorded for seeds from saline conditions under the 25/15 °C treatment. Additionally, percent germination was significantly lower for the seeds incubated in the saline solutions (100, 200, 400, and 600 mmol/L NaCl) and germinated under colder conditions (15/25 °C), compared with the seeds incubated in non-saline solutions (control group, 0 mmol/L NaCl) and germinated under warmer conditions (35/25 °C). The highest recovery percentage was recorded for seeds of the hyper-saline habitat incubated at 35/25 °C. Thus, seeds maintained their viability despite experiencing a range of saline conditions and were able to germinate upon the arrival of suitable conditions, which can be an adaptation to its saline arid desert habitat.

scholarly journals Maturation and germination of Vernonanthura discolor seeds

2016 ◽  
Vol 40 (2) ◽  
pp. 164-172
Author(s):  
Camila Ribeiro de Souza Grzybowski ◽  
Rosemeire Carvalho da Silva ◽  
Elisa Serra Negra Vieira ◽  
Maristela Panobianco
Keyword(s):  
Native Species ◽  
Dry Matter ◽  
Reproductive Development ◽  
Dry Weight ◽  
Harvest Time ◽  
Germination Test ◽  
Physiological Maturity ◽  
Light Regimes ◽  
Blotting Paper ◽  
Periodic Monitoring

ABSTRACT Vernonanthura discolor is a native species with potential for reclamation of degraded lands, and the manufacture of wood panels. This study has aimed to evaluate the process of maturation and germination of Vernonanthura discolor seeds, determining the harvest time, and conducting the germination test. A periodic monitoring of the reproductive development of sources of Vernonanthura discolor was conducted, located in the municipality of Bocaiúva do Sul, PR, Brazil; and starting from the anthesis, weekly collections of seeds were performed to evaluate the percentage of dry matter, germinating power, and vigor, in order to identify the physiological maturity of the seeds. In the germination test, two combinations of four temperatures (20, 30, 20-30 and 30 °C), and two light regimes were tested, seeking to define the best methodology and evaluation dates of the test. It was concluded that the seeds physiological maturity Vernonanthura discolor reached 45 days after the anthesis, when the maximum values of dry weight, germination and vigor are achieved. Germination test may be conducted on blotting paper at 20 or 25 °C with a supply of light, or at 25 °C in the dark, the first count being on the 13th and the last one on the 29th day after seeding.

scholarly journals Rate and Synchrony of Seed Germination Influence Growth of Hydroponic Lettuce

HortScience ◽  
1999 ◽  
Vol 34 (1) ◽  
pp. 100-103
Author(s):  
Ahmet Korkmaz ◽  
Wallace G. Pill ◽  
Bruce B. Cobb
Keyword(s):  
Seed Germination ◽  
Growth Response ◽  
Germination Rate ◽  
Seedling Emergence ◽  
Time Range ◽  
Fresh Weight ◽  
Shoot Fresh Weight ◽  
Seedling Length ◽  
Emergence Rate ◽  
Germinated Seeds

The effect of seed germination rate, or of seedling emergence rate, was studied in relation to subsequent plant growth of `Cortina' lettuce (Lactuca sativa L.). Seedling growth response to selection by time of germination was assessed by imbibing seeds at 5 °C to increase the time range for germination. Germinated seeds were removed daily and transferred to “slants” (germination paper held at 20° from vertical) at 15 °C. Five days after each transfer, root and hypocotyl lengths were measured. As days required for germination increased, root lengths decreased and hypocotyl lengths increased, resulting in no change in total seedling length. The relation between rate of seedling emergence from raw or pelleted seeds of the same lot and shoot fresh weight was examined using commercially practiced hydroponic techniques. Shoot fresh weight at 10 and 21 days after planting was related inversely and linearly to the day of emergence for both seed treatments. In the same study, the coefficient of variation of shoot fresh weight was positively related to time of seedling emergence only at 10 days. Germinated seeds were selected after 1 and 2 days of imbibition; subsequent seedling emergence rate and shoot fresh weight at 25 days were recorded. First-day germinated seeds had faster and more synchronous emergence, and produced heavier and more uniform shoots. Discarding slow-to-germinate seeds should enhance seedling emergence and growth.

Chloride salts inhibit emergence and seedling growth of chickpea rather than germination

2017 ◽  
Vol 40 (04) ◽  
Author(s):  
Hamdi Özaktan ◽  
Cemalettin Yasar Çiftçi Çiftçi ◽  
Mehmet Demir KAYA ◽  
Sati Uzun ◽  
Oguzhan Uzun ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Seedling Growth ◽  
Genotypic Variation ◽  
Germination Percentage ◽  
Distilled Water ◽  
Germination Test ◽  
Seedling Length ◽  
Mean Germination Time ◽  
Chloride Salts ◽  
Chickpea Cultivars

The effects of chloride salts (NaCl, MgCl2 and CaCl2) with the same electrical conductivity (EC) values on germination, emergence, seedling growth and Cl content of seedling in chickpea cultivars (Akçin 91, Aziziye, Gökçe, Inci, Iºýk-05 and Yaºa-05) were investigated. The EC values of the chloride salts were adjusted to 4, 8 and 16 dS m-1 and the distilled water was served as control. Germination percentage, mean germination time, radicula and plumula lengths, fresh and dry radicula and plumula weight, chloride (Cl-) content of seedling in germination test, and seedling length, fresh and dry seedling weight in emergence test were measured. The results revealed that increasing salt doses caused a significant reduction in parameters of all cultivars. The dose of 16 dS m-1 from each salt was found to be lethal. Cl- content of seedling was increased from 0.05 ppm in control to 4.10 ppm in 16 dS m-1; resulting in decreasing germination, emergence and seedling growth. The cultivar Aziziye presented better performance and gave higher emergence rates in NaCl, while Iºýk-05 in CaCl2 and Gökçe in MgCl2; indicating that there was a genotypic variation towards different salt sources. It was concluded that emergence and seedling growth were severely influenced by increasing salinity without changing germination and emergence was not possible at 16 dS m-1.

Seed germination and seedling dry matter production of canola, barley and wheat as affected by seed-placed KCl and polymer-coated KCl

1998 ◽  
Vol 78 (4) ◽  
pp. 611-614 ◽  
Author(s):  
Mingchu Zhang ◽  
M. Nyborg ◽  
E. D. Solberg
Keyword(s):  
Adverse Effect ◽  
Seed Germination ◽  
Key Words ◽  
Seedling Growth ◽  
Dry Matter ◽  
Dry Matter Production ◽  
Greenhouse Experiments ◽  
Matter Production ◽  
Salt Index ◽  
Germinated Seeds

Seed-placed KCl often adversely affects seed germination and seedling growth because of the high salt index of the material. By coating KCl granules the adverse effect of the KCl can be reduced. Two greenhouse experiments with canola, barley and wheat, and one experiment in the field with barley were conducted to determine if normally adverse levels of seed-placed KCl fertilizer could be reduced when polymer-coated KCl is used. The results show that the number of germinated seeds and seedling dry matter were higher with the seed-placed coated KCl than the seed-placed regular non-coated KCl. Key words: KCl fertilizer, polymer-coated KCl, germination, seedling dry matter

SEED GERMINATION AND SEEDLING GROWTH CHARACTERISTICS OF ATRAZINE-SUSCEPTIBLE AND RESISTANT BIOTYPES OF Brassica campestris

1982 ◽  
Vol 62 (3) ◽  
pp. 733-739 ◽  
Author(s):  
L. R. MAPPLEBECK ◽  
V. SOUZA MACHADO ◽  
B. GRODZINSKI
Keyword(s):  
Seed Germination ◽  
Water Content ◽  
Seedling Growth ◽  
Dry Matter ◽  
Brassica Campestris ◽  
Early Stage ◽  
Growth Characteristics ◽  
Significant Difference ◽  
Seedling Characteristics ◽  
Germinated Seeds

Seed germination and seedling characteristics of atrazine-susceptible and resistant wild turnip rape biotypes (Brassica campestris L.) were examined. Seeds of the susceptible biotype germinated earlier, and the germinated seeds grew faster and emerged earlier than seeds of the resistant biotype. Seedlings of the susceptible biotype emerged from greater planting depths than the resistant seedlings. Emerged susceptible seedlings were larger, with longer hypocotyls and greater cotyledon area, and produced more dry matter than the resistant biotype seedlings. Differences in water content of germinated seeds of the two biotypes were noted, with the susceptible biotypes consistently showing a higher water content. There was no significant difference in the respiration rate of the two biotypes at the early stage of seedling growth.

Overcoming Dormancy and Influence of Light on the Physiological Quality of Senna cana (Fabaceae) Seeds

2019 ◽  
pp. 1-9
Author(s):  
Jordânia X. Medeiros ◽  
Ana L. P. Feliciano ◽  
Valderez P. Matos ◽  
Girlânio H. Silva ◽  
Yana S. Lopes ◽  
...  
Keyword(s):  
Seed Germination ◽  
Seed Dormancy ◽  
Native Species ◽  
Red Light ◽  
Distilled Water ◽  
Light Regimes ◽  
Germination Process ◽  
Physiological Quality ◽  
Mean Germination Time

Environmental factors affect the germination process, like the presence of seed coat and the quality of light; these informations are still scarce for many native species from Brazil, especially for Senna cana, which there are no adequate standards and methodologies to be used in germination tests. The aim of this research was to recommend adequate pre-germinative treatment(s) to overcome seed dormancy, and determine the degree of influence of different light regimes in seed germination of S. cana. Two experiments were carried out: T1-evaluation of different methods of dormancy overcoming (intact seeds (control), T2-imbibition of the intact seeds for 24 hours (in distilled water), T3-scarified seeds with sandpaper n° 100 in the hilum opposite region, T4-scarified seeds with sandpaper n° 100 in the region the hilo opposite region and imbibition in water (in distilled water) for 24 hours; T5-imbibition in water at 80°C); 2-Influence of light quality on seed germination and vigor (white light, red light, far red light and absence of light). The evaluated parameters were: first germination count, percentage of germination, IVG (Germination speed index), MGT (Mean germination time). Treatments were compared by Tukey at 5% probability. The best method for overcoming seed dormancy was mechanical scarification with sandpaper nº 100. The germination of the seeds of S. cana can be classified as indifferent to the luminosity, despite germinative response be greater under white and red light.

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