EFFECTS OF SOIL TEMPERATURE ON PHOSPHORUS EXTRACTABILITY. I. EXTRACTIONS AND PLANT UPTAKE OF SOIL AND FERTILIZER PHOSPHORUS

1984 ◽  
Vol 64 (2) ◽  
pp. 241-254 ◽  
Author(s):  
S. C. SHEPPARD ◽  
G. J. RACZ

The effect of 10, 15, 20 and 25 °C soil temperatures on the extractability of soil and fertilizer phosphorus (P) was examined in two soils, one containing free carbonate (pH 7.8) and the other non-carbonated (pH 6.9). The time course of fixation and desorption reactions were monitored. The extractability of P was also assessed using sodium bicarbonate (NaHCO3) extractions, desorption curves, and short-term uptake by wheat seedlings. Phosphorus-32 was used throughout. Opposing effects of temperature were found. An increase in incubation temperature from 10 to 25 °C decreased the amount of applied P extracted probably due to accelerated fixation reactions. This effect was established 1 day after the P was applied and persisted for 57 days. An increase in extraction temperature over the corresponding incubation temperature increased the extractability of P, indicating endothermic desorption reactions. This effect was established 1 h after the extraction began and persisted for 48 h. Hence, the net effect of temperature on the extractability of P will depend upon the balance of these opposing processes. The time course of these processes had two phases. The effects of temperature were established during the initial phase (< 1 day) of each process. The reactions continued more slowly after the initial phase but the later phases were not significantly affected by temperature. The P-desorption buffer capacity increased as temperature increased in the fertilized, carbonated soil. Isotopic exchange of applied 32P with native soil 31P increased as temperature increased. Isotopic exchange appeared to be more extensive when measured by plant uptake as opposed to NaHCO3 extraction, suggesting that the plants had access to a larger pool of soil 31P. Key words: Temperature, soil phosphorus, fertilizer, incubation, desorption, plant uptake, wheat

1996 ◽  
Vol 86 (6) ◽  
pp. 715-720 ◽  
Author(s):  
Martin P. Wellby ◽  
Matthew Baylis ◽  
Peter Rawlings ◽  
Philip S. Mellor

AbstractCulicoides variipennis sonorensis Wirth & Jones and C. nubeculosus (Meigen) were orally infected with African horse sickness virus (AHSV) type 9 and subsequently incubated at 10, 15, 20 and 25°C (R.H. 80%±10%). A time course of infection rates and virus titres was recorded by assaying flies individually or in pools, and survival rates of flies were also estimated. Survival rates at 10, 15 and 20°C were very similar and 80–90% of flies remained alive after 14 days; at 25°C after the same period survival was reduced to 40%. None of the C. nubeculosus became persistently infected with AHSV, but the virus took longer to clear as the incubation temperature dropped. At temperatures of 10, 15, 20 and 25°C virus was undetectable on days 12, 8, 5, and 4 days post infection (dpi), respectively. In C. v. sonorensis both the infection rate and rate of virogenesis were related to temperature. At 25°C a maximum mean titre of 104.3 TCID50/fly was reached by 9 dpi and the infection rate remained between 60 and 80%. At 20°C virogenesis was slower and a maximum mean titre of 104.3 TCID50/fly was reached only after 23 days; the infection rate was also reduced to 50–70%. At 15°C there was an overall decline in virus titre with time, although between 12 and 15 dpi some pools of flies contained 103.0–104.0 TCID50/fly, demonstrating that virogenesis can occur. The infection rate at this temperature decreased dramatically to 0–15% after 9 dpi. At 10°C there was no detectable virogenesis and all pools tested at 13 dpi were negative. The apparent infection rate dropped to 0–5% between 13 and 35 days post infection. However, when surviving flies were then returned to 25 °C for 3 days the infection rate increased to 15.5%. It therefore appears that at low temperatures the virus does not replicate but infectious virus may persist at a level below that detectable by the usual assay systems. The implications of these findings for the epidemiology of AHS are discussed.


2002 ◽  
Vol 48 (11) ◽  
pp. 2017-2022 ◽  
Author(s):  
Diane M Hill ◽  
Lisa J Johnson ◽  
Paul J Burns ◽  
Angela M Neale ◽  
Denise M Harmening ◽  
...  

Abstract Background: The accuracy of homocysteine (Hcy) results is currently compromised by the requirement to separate the plasma within 1 h of sample collection. We studied the effect of temperature on the stability of plasma Hcy over a 72-h time course in blood collected into evacuated tubes containing either EDTA alone or both EDTA and 3-deazaadenosine (3DA). Methods: We recruited 100 volunteers, including both diseased and healthy individuals with a range of baseline plasma Hcy values, from two centers. Blood samples were collected into tubes containing EDTA, and EDTA plus 3DA and stored at ambient temperature (20–25 °C) or refrigerated (2–8 °C). Aliquots of blood were centrifuged at various times up to 72 h, the plasma was removed, and Hcy was measured by HPLC. Results: Plasma Hcy measurement covering the sample collection and storage conditions during the whole time course was possible on samples from 59 of those recruited. One-way ANOVA for repeated measures within subjects revealed that only samples that were collected into tubes containing EDTA plus 3DA and stored refrigerated were stable over 72 h (P = 0.2761). Conclusions: A combination of 3DA and storage at 2–8 °C will allow collection of samples for plasma Hcy measurement outside of the hospital setting and wider population screening.


1984 ◽  
Vol 64 (2) ◽  
pp. 255-263 ◽  
Author(s):  
S. C. SHEPPARD ◽  
G. J. RACZ

The change in the extractability of soil phosphorus (P) in response to temperature was examined in 12 Manitoba soils. These soils varied in carbonate and P contents. The soil P was labelled with 32P to facilitate measurements. Sodium bicarbonate extractions, anion exchange resin extractions, P desorption curves and short-term plant uptake using wheat were used to measure P extractability. An increase in soil temperature increased the extractability of P. This was apparent for P extracted by NaHCO3 only in soils low in P. The P extracted by resin appeared to respond similarly but was quite variable. Effects of temperature on the desorption curve parameters were significant only in soils high in P. The latter may reflect the detection limits for P using the desorption curve extraction system. Plant uptake was closely correlated to root growth. Both increased markedly as temperature increased. However, in certain soils the increase in P uptake due to temperature was far greater than the corresponding increase in root growth. The estimates of the labile pool accessed by plants increased as temperature increased. The principle hypothesis, that the effect of temperature on P extractability changed from soil to soil, was confirmed. The only controlling soil factor that could be identified was the basic soil P content. Key words: Temperature, soil phosphorus, carbonated, non-carbonated, plant uptake, wheat


MRS Advances ◽  
2020 ◽  
Vol 5 (63) ◽  
pp. 3389-3395
Author(s):  
R. González-Díaz ◽  
D. Fernández-Sánchez ◽  
P. Rosendo-Francisco ◽  
G. Sánchez-Legorreta

AbstractIn this work, the first results of the effects of temperature during the production of Se2- ions and the effect during the interaction of Cd2+ and Se2- ions in the synthesis process of CdSe nanoparticles are presented. The synthesis of CdSe was carried out by the colloidal technique, in the first one we used a temperature of 63 °C to produce Se2- ions and in the second one an interaction temperature of 49 °C. The samples were characterized using a Scanning Electron Microscope (SEM) and a Scanning Tunneling Microscope (STM). From the SEM micrographs it was possible to identify the thorns formation and irregular islands. STM micrographs reveal elliptical shapes with a regular electron cloud profile.


Weed Science ◽  
1979 ◽  
Vol 27 (6) ◽  
pp. 595-598 ◽  
Author(s):  
T. V. Toai ◽  
D. L. Linscott

We studied the effects of temperature (5, 10, 20, and 30 C) on the phytotoxic activity of decaying quackgrass [Agropyron repens (L.) Beauv.] leaves and rhizomes that were incubated in soils for 0, 1, 2, 4, and 6 weeks. Alfalfa (Medicago sativa L.) seeds were grown for 96 h in water, water extracts of control soils, and water extracts of soil with quackgrass rhizomes or leaves. Dried quackgrass rhizomes and leaves contained water-soluble toxins that inhibited alfalfa seedling development and growth. There was a strong interaction between incubation time and temperature on the development of additional toxins by decomposing quackgrass. High incubation temperature (30 C) accelerated toxin formation and ultimate decay. Intermediate temperature (20 C) delayed toxin formation and decay. Low incubation temperatures (5 C and 10 C) prevented formation of additional toxin. In all extracts of quackgrass and soil that had been incubated for 6 weeks, normal alfalfa seedling number equaled that in water. However, seedling growth varied with incubation temperatures.Treatment of quackgrass with glyphosate [N-(phosphonomethyl) glycine] in the greenhouse did not influence the toxicity of decaying quackgrass leaves. The highest toxic effect was noted after 1 week of decay on the soil surface.


2009 ◽  
Vol 40 (1-6) ◽  
pp. 141-149 ◽  
Author(s):  
Kazi Suleman Memon ◽  
Abdul Jaleel Jarwar ◽  
Mehruinsa Memon ◽  
Zia‐ul‐hassan

1999 ◽  
Vol 82 (3) ◽  
pp. 1198-1208 ◽  
Author(s):  
Kaoru Yoshida ◽  
Yoshiki Iwamoto ◽  
Sohei Chimoto ◽  
Hiroshi Shimazu

Omnipause neurons (OPNs) are midline pontine neurons that are thought to control a number of oculomotor behaviors, especially saccades. Intracellular recordings were made from OPNs in alert cats to elucidate saccade-associated postsynaptic events in OPNs and thereby determine what patterns of afferent discharge impinge on OPNs to cause their saccadic inhibition. The membrane potential of impaled OPNs exhibited steep hyperpolarization before each saccade that lasted for the whole period of the saccade. The hyperpolarization was reversed to depolarization by intracellular injection of Cl− ions, indicating it consisted of temporal summation of inhibitory postsynaptic potentials (IPSPs). The duration of the saccade-related hyperpolarization was almost equal to the duration of the concurrent saccades. The time course of the hyperpolarization was similar to that of the radial eye velocity except for the initial phase. During the falling phase of eye velocity, the correlation between the instantaneous amplitude of hyperpolarization and the instantaneous eye velocity was highly significant. The amplitude of hyperpolarization at the eye velocity peak was correlated significantly with the peak eye velocity. The time integral of the hyperpolarization was correlated with the radial amplitude of saccades. The initial phase disparity between the hyperpolarization and eye velocity was due to the relative constancy of peak time (∼20 ms) of the initial steep hyperpolarization regardless of the later potential profile that covaried with the eye velocity. The initial steep hyperpolarization led the beginning of saccades by 15.9 ± 3.8 (SD) ms, which is longer than the lead time for medium-lead burst neurons. These results demonstrate that the pause of activity in OPNs is caused by IPSPs initiated by an abrupt, intense input and maintained, for the whole duration of the saccade, by afferents conveying eye velocity signals. We suggest that the initial sudden inhibition originates from central structures such as the superior colliculus and frontal eye fields and that the eye velocity-related inhibition originates from the burst generator in the brain stem.


2021 ◽  
Vol 9 (2) ◽  
pp. 186
Author(s):  
Dimas Anggi Ananta ◽  
G.P. Ganda Putra ◽  
I Wayan Arnata

Cocoa pod husk is a by-product of cocoa processing which is quite abundant and has not been used optimally. Cacao pod husk can be used more optimally by extracting, its content of polyphenol compounds which can be used as natural antioxidants. The aim of this study were to determine the effect of temperature and maceration time of cocoa pod husk extract as a source of antioxidants and to determine the best type of temperature and maceration time to produce cocoa pod husk extract as a source of antioxidants. This experiment was designed by using factorial randomized block design. The first factor was type of maceration temperature consisting of 30±2°C, 45±2°C and 60±2°C. The second factor was maceration time, which were done for 24, 36 and 48 h. The data were analyzed with analysis of variance and continued with the Tukey test. The results showed that the temperature and time of maceration had a very significant effect on yield, total phenolic and antioxidant capacity of cocoa pod husk extract. Interactions between treatments had a very significant effect on total phenolic and antioxidant capacity but did not significantly affect the yield of cocoa pod husk extract. The best treatment for producing cocoa pod husk extract as a source of antioxidants was using maceration temperature 60±2°C and maceration time for 36 h with yield characteristics 5,28±0,15%, total phenolic at 168.16±0,06 mg GAE/g and capacity antioxidant 130.94±0.84 mg GAEAC/g. Keywords: Cocoa pod husk, extraction, temperature, time, antioxidants.


2020 ◽  
Vol 287 (1932) ◽  
pp. 20200992 ◽  
Author(s):  
Anthony T. Breitenbach ◽  
Amanda W. Carter ◽  
Ryan T. Paitz ◽  
Rachel M. Bowden

Most organisms are exposed to bouts of warm temperatures during development, yet we know little about how variation in the timing and continuity of heat exposure influences biological processes. If heat waves increase in frequency and duration as predicted, it is necessary to understand how these bouts could affect thermally sensitive species, including reptiles with temperature-dependent sex determination (TSD). In a multi-year study using fluctuating temperatures, we exposed Trachemys scripta embryos to cooler, male-producing temperatures interspersed with warmer, female-producing temperatures (heat waves) that varied in either timing during development or continuity and then analysed resulting sex ratios. We also quantified the expression of genes involved in testis differentiation ( Dmrt1 ) and ovary differentiation ( Cyp19A1 ) to determine how heat wave continuity affects the expression of genes involved in sexual differentiation. Heat waves applied during the middle of development produced significantly more females compared to heat waves that occurred just 7 days before or after this window, and even short gaps in the continuity of a heat wave decreased the production of females. Continuous heat exposure resulted in increased Cyp19A1 expression while discontinuous heat exposure failed to increase expression in either gene over a similar time course. We report that even small differences in the timing and continuity of heat waves can result in drastically different phenotypic outcomes. This strong effect of temperature occurred despite the fact that embryos were exposed to the same number of warm days during a short period of time, which highlights the need to study temperature effects under more ecologically relevant conditions where temperatures may be elevated for only a few days at a time. In the face of a changing climate, the finding that subtle shifts in temperature exposure result in substantial effects on embryonic development becomes even more critical.


2020 ◽  
Author(s):  
Lei Qin ◽  
Qiang Sun ◽  
Jiani Shao ◽  
Yang Chen ◽  
Xiaomei Zhang ◽  
...  

Abstract Background: The effects of temperature and humidity on the epidemic growth of coronavirus disease 2019 (COVID-19)remains unclear.Methods: Daily scatter plots between the epidemic growth rate (GR) and average temperature (AT) or average relative humidity (ARH) were presented with curve fitting through the “loess” method. The heterogeneity across days and provinces were calculated to assess the necessity of using a longitudinal model. Fixed effect models with polynomial terms were developed to quantify the relationship between variations in the GR and AT or ARH.Results: An increased AT dramatically reduced the GR when the AT was lower than −5°C, the GR was moderately reduced when the AT ranged from −5°C to 15°C, and the GR increased when the AT exceeded 15°C. An increasedARH increased theGR when the ARH was lower than 72% and reduced theGR when the ARH exceeded 72%.Conclusions: High temperatures and low humidity may reduce the GR of the COVID-19 epidemic. The temperature and humidity curves were not linearly associated with the COVID-19 GR.


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