scholarly journals Ultrastructural Analysis of the Ontogenetic Development of Shoot Induced from Embryonic Axes of Costa Rican Bean Varieties (<i>Phaseolus vulgaris</i> L.) under <i>in Vitro</i> Conditions by Scanning Electronic Microscopy

2012 ◽  
Vol 03 (04) ◽  
pp. 489-494 ◽  
Author(s):  
Marisol Jiménez ◽  
Andrés Gatica ◽  
Ethel Sánchez ◽  
Marta Valdez
Keyword(s):  
Phaseolus Vulgaris ◽  
Costa Rican ◽  
Ultrastructural Analysis ◽  
Ontogenetic Development ◽  
Scanning Electronic Microscopy ◽  
Electronic Microscopy ◽  
Embryonic Axes

WHEAT ANDROCLINIC CALLI: DATA OF SCANNING ELECTRONIC MICROSCOPY

2021 ◽  
Vol 0 (4) ◽  
pp. 41-47
Author(s):  
O.A. SELDIMIROVA ◽  
Keyword(s):  
Culture Medium ◽  
Scanning Electronic Microscopy ◽  
Electronic Microscopy ◽  
Spring Soft Wheat ◽  
Fundamental Possibility ◽  
Morphological Status ◽  
Different Types ◽  
Morphogenesis In Vitro ◽  
Morphogenic Callus

The processes of formation different types of calli, as well as the morphogenesis pathways in morphogenic calli, were studied by scanning electron microscopy (SEM) during anther culture in vitro in hybrid line Fotos of spring soft wheat. The microspore haploid origin of calli has been proven. The morphological status of the obtained calli was determined. It was shown that morphogenic callus consists of small densely packed meristematic cells covered with extracellular substance. This type of calli was obtained using a variant of the Potato II induction culture medium, added by 1.0 mg/l synthetic auxin 2,4-D. Nonmorphogenic callus consists of large, elongated, loosely located cells with a smooth surface. This type of calli was obtained using a variant of the Potato II culture medium, added by 2.0 mg/l 2,4-D. It was found that the introduction of various IAA concentrations into the Blaydes nutrient medium for regeneration in morphogenic calli implements the following pathways of morphogenesis in vitro: embryoidogenesis (without IAA addition), gemmorhizogenesis (0.5 mg/l), and rhizogenesis (1.5 mg/l). Revealed degenerative changes in cells of nonmorphogenic calli. The fundamental possibility of regulating of the morphogenesis pathways of in vitro of morphogenic calli in the direction necessary for research in biotechnological research has been confirmed.

Synthesis of Selenium Nanoparticles in the Presence of Oyster Polysaccharides and the Antioxidant Activity

2014 ◽  
Vol 522-524 ◽  
pp. 1143-1146
Author(s):  
Meng Tao Yang ◽  
Feng Li ◽  
Yu Chao Wang ◽  
Q.Y. Liu
Keyword(s):  
Antioxidant Activity ◽  
Sodium Selenite ◽  
Soft Template ◽  
Radical Scavenger ◽  
Dpph Radical ◽  
Scanning Electronic Microscopy ◽  
Electronic Microscopy ◽  
Simple Method ◽  
Electronic Microscope

A simple method for synthesis of nanoselenium using oyster polysaccharides as soft template was investigated. The uniform stable selenium polysaccharides were obtained under the condition of reaction temperature 40°C, reaction time 3 h-5 h, the content of oyster polysaccharides 400mg/L and the content of sodium selenite 1mM. The size distribution and morphology of the product were confirmed by scanning electronic microscopy (SEM) and tansmission electronic microscope (TEM). The antioxidant activity of nanoselenium polysaccharides were studied in vitro. The results showed that nanoselenium polysaccharide is an effective hydroxyl radical and DPPH radical scavenger.

scholarly journals Critical Factors for Metformin Osmotic Controlled Release Pump

2020 ◽  
pp. 1-12
Author(s):  
García-Flores Mirna ◽  
Melgoza-Contreras Luz María ◽  
Hernández-Baltazar Efrén
Keyword(s):  
Drug Release ◽  
Release Rate ◽  
Osmotic Pump ◽  
Critical Factors ◽  
Scanning Electronic Microscopy ◽  
Electronic Microscopy ◽  
Drug Release Profile ◽  
Osmotic Pumps ◽  
The Impact

Aim: Studied the critical factors in the design of an osmotic pump with metformin release rate constant at 4%/hr in diabetes mellitus within 24 hr. with the goal to reduce daily medications. Study Design: Experimental design 32 Methodology: In vitro drug release profiles for 24h. The effects of different formulation variables, that is, concentration of hydrophilic polymer, diameter of drug releasing orifice and coating thickness, on the drug release profile were investigated. Also, the impact of pH, osmotic pressure and morphology with stereo microscopy and scanning electronic microscopy of the osmotic pumps were investigated. At last, osmotic pumps surface was analyze with scanning electronic microscopy. Results: Metformin osmotic pump were successfully prepared in this study to overcome the weak point of multiple doses and great concentration fluctuation of metformin. The formulation determined finally have a release orifice of 700 mm and 3.0% of weight gain, achieved the desired effect which can realize the constant drug release rate at the first 24 hr. Conclusion: The developed osmotic systems have a linear release near 4%/hr. and demonstrated that the behavior was independent of the agitation intensity and the pH of the gastrointestinal apparatus.

scholarly journals Release of the Diclofenac Sodium by Nanofibers of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Obtained from Electrospinning and Solution Blow Spinning

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Michelle Andrade Souza ◽  
Karine Yamamura Sakamoto ◽  
Luiz Henrique Capparelli Mattoso
Keyword(s):  
Diclofenac Sodium ◽  
X Ray Diffraction ◽  
Fiber Morphology ◽  
Scanning Electronic Microscopy ◽  
Electronic Microscopy ◽  
Polymer Nanofibers ◽  
Scanning Calorimetry ◽  
X Ray ◽  
Solution Blow Spinning

Electrospun fibers are explored as a new system for controlled drug delivery. Novel techniques capable of obtaining polymer nanofibers have been reported in the literature. They include solution blow spinning (SBS), which is a technique to produce polymer nanofibers in the same range as electrospinning, using pressurized gas instead of high voltage. The present study investigates release characteristics of diclofenac sodium encapsulated at three concentrations (5, 10, and 20% w/v) in poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanofibers made by electrospinning and SBS and determines the drug’s effect on fiber morphology and structural properties. PHBV nanofibers were characterized using scanning electronic microscopy, differential scanning calorimetry, and X-ray diffraction, and the release profile was examined via UV-Vis spectrophotometry. Both electrospinning and SBS encapsulated diclofenac sodium in PHBV membranes efficiently and effectively. The profile of thein vitrorelease of diclofenac sodium was dependent on drug concentration and temperature. The drug reduced crystallinity and increased flexibility.

In vitro therapeutic properties of different fractions of Phaseolus vulgaris seeds as affected by the distribution of phytochemicals

2017 ◽  
Vol 42 (2) ◽  
pp. e12485
Author(s):  
Usha Rani Telikicherla ◽  
Mahadeva Naika ◽  
Anilakumar Kandangath Raghavan ◽  
Sashikala Vadakkoot Balakrishnan
Keyword(s):  
Phaseolus Vulgaris ◽  
Therapeutic Properties

scholarly journals Tribological Performance of CoCrMo Alloys with Boron Additions in As-Cast and Heat-Treated Conditions

Metals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 355
Author(s):  
Marco A. L. Hernandez-Rodriguez ◽  
Diego E. Lozano ◽  
Gabriela M. Martinez-Cazares ◽  
Yaneth Bedolla-Gil
Keyword(s):  
Boron Content ◽  
Tribological Performance ◽  
Secondary Phases ◽  
Scanning Electronic Microscopy ◽  
Electronic Microscopy ◽  
Hardness Tests ◽  
Heat Treated ◽  
Cocrmo Alloys ◽  
Cast Condition ◽  
Ball On Disc

The present study evaluates the effect of boron additions on the tribological performance of CoCrMo alloys. The alloys were prepared with boron ranging from 0.06 to 1 wt%. The materials were characterized using metallographic techniques, scanning electronic microscopy, and roughness and hardness tests. Tribological evaluation was made by means of ball-on-disc tests for sliding distances of 4, 8 and 12 km. The samples were in the as-cast condition and after a heat treatment at 1200 °C for 1 h, finished by water quenching. The results showed that wear resistance was influenced by the microstructure and the number of secondary phases. The volume loss decreased as the boron content increased. Due to hard phases, abrasion wear was observed. Delamination fatigue was also detected after long sliding distances. Both wear mechanisms diminished in higher boron content alloys.

scholarly journals Potencial in vitro de Bacillus spp. no controle de Curtobacterium flaccumfaciens pv. flaccumfaciens em feijoeiro-comum

2016 ◽  
Vol 42 (4) ◽  
pp. 360-362
Author(s):  
Evelynne Urzêdo Leão ◽  
◽  
Júlio Cesar da Silva ◽  
Fabíola Rodrigues Medeiros ◽  
Gabriela Silva Santa Rosa Macêdo ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Bacillus Licheniformis ◽  
Bacillus Spp

RESUMO O feijoeiro-comum (Phaseolus vulgaris) é uma cultura de grande relevância na alimentação da população brasileira. A murcha-de-curtobacterium ou murcha bacteriana, causada por Curtobacterium flaccumfaciens pv. flaccumfaciens (Cff). é uma doença vascular que acomete o feijoeiro causando danos significativos. Neste contexto, o objetivo deste trabalho foi avaliar a ação in vitro de Bacillus spp. na inibição de dois isolados de Cff, colonização do sistema radicular e desenvolvimento de plântulas de feijoeiro-comum. Foram realizados dois ensaios in vitro para verificar a atividade antagônica dos isolados Bacillus licheniformis, B. subtilis e B. subtilis + B. lichenformis a dois isolados de Cff. Todos os isolados de Bacillus spp. apresentaram inibição no crescimento dos isolados de Cff. Não foi observada a colonização das raízes das plântulas de feijoeiro-comum, pelos isolados bacterianos avaliados.

Sign in / Sign up

Export Citation Format

Share Document