Sodium Fluoride Induces Hepato-Renal Oxidative Stress and Pathophysiological Changes in Experimental Animals

Abstract Background Fluoride may induce oxidative stress and apoptosis. It may also lead to neurobehavioural defects including neuromuscular damage. The present study aimed to explore the effects of sub lethal concentrations of sodium fluoride (NaF) on the lifespan and climbing ability of Drosophila melanogaster. In total, 0.6 mg/L and 0.8 mg/L of NaF were selected as sublethal concentrations of NaF for the study. Lifespan was measured and climbing activity assay was performed. Results The study showed significant decrease in lifespan of flies treated with fluoride. With increasing age, significant reduction in climbing activity was observed in flies treated with sodium fluoride as compared to normal (control) flies. Flies treated with tulsi (Ocimum sanctum) and NaF showed increase in lifespan and climbing activity as compared to those treated with NaF only. Lipid peroxidation assay showed significant increase in malondialdehyde (MDA) values in the flies treated with NaF as compared to control. The MDA values decreased significantly in flies treated with tulsi mixed with NaF. Conclusions The results indicate that exposure to sub lethal concentration of NaF may cause oxidative stress and affect the lifespan and climbing activity of D. melanogaster. Tulsi extract may help in reducing the impact of oxidative stress and toxicity caused by NaF.

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In Vivo Protective Effects of Gallic Acid Isolated from Peltiphyllum Peltatum Against Sodium Fluoride-Induced Oxidative Stress in Rat Erythrocytes

Archives of Industrial Hygiene and Toxicology ◽

10.2478/10004-1254-64-2013-2353 ◽

2013 ◽

Vol 64 (4) ◽

pp. 553-559 ◽

Cited By ~ 12

Author(s):

Seyed Fazel Nabavi ◽

Solomon Habtemariam ◽

Antoni Sureda ◽

Akbar Hajizadeh Moghaddam ◽

Maria Daglia ◽

...

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Superoxide Dismutase ◽

Vitamin C ◽

Gallic Acid ◽

Sodium Fluoride ◽

Enzyme Activities ◽

Control Group ◽

Rat Erythrocytes ◽

Pre Treatment

Abstract Gallic acid has been identified as an antioxidant component of the edible and medicinal plant Peltiphyllum peltatum. The present study examined its potential protective role against sodium fluoride (NaF)-induced oxidative stress in rat erythrocytes. Oxidative stress was induced by NaF administration through drinking water (1030.675 mg m-3 for one week). Gallic acid at 10 mg kg-1 and 20 mg kg-1 and vitamin C for positive controls (10 mg kg-1) were administered daily intraperitoneally for one week prior to NaF administration. Thiobarbituric acid reactive substances, antioxidant enzyme activities (superoxide dismutase and catalase), and the level of reduced glutathione were evaluated in rat erythrocytes. Lipid peroxidation in NaF-exposed rats significantly increased (by 88.8 %) when compared to the control group (p<0.05). Pre-treatment with gallic acid suppressed lipid peroxidation in erythrocytes in a dose-dependent manner. Catalase and superoxide dismutase enzyme activities and glutathione levels were reduced by NaF intoxication by 54.4 %, 63.69 %, and 42 % (p<0.001; vs. untreated control group), respectively. Pre-treatment with gallic acid or vitamin C significantly attenuated the deleterious effects. Gallic acid isolated from Peltiphyllum peltatum and vitamin C mitigated the NaF-induced oxidative stress in rat erythrocytes.

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Neuroprotective effects of silymarin on sodium fluoride-induced oxidative stress

Journal of Fluorine Chemistry ◽

10.1016/j.jfluchem.2012.06.029 ◽

2012 ◽

Vol 142 ◽

pp. 79-82 ◽

Cited By ~ 15

Author(s):

Seyed Mohammad Nabavi ◽

Antoni sureda ◽

Seyed Fazel Nabavi ◽

Ali Mohammad Latifi ◽

Akbar Hajizadeh Moghaddam ◽

...

Keyword(s):

Oxidative Stress ◽

Sodium Fluoride ◽

Neuroprotective Effects

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Protective Role of Gallic Acid on Sodium Fluoride Induced Oxidative Stress in Rat Brain

Bulletin of Environmental Contamination and Toxicology ◽

10.1007/s00128-012-0645-4 ◽

2012 ◽

Vol 89 (1) ◽

pp. 73-77 ◽

Cited By ~ 39

Author(s):

Seyed Fazel Nabavi ◽

Solomon Habtemariam ◽

Mahtab Jafari ◽

Antoni Sureda ◽

Seyed Mohammad Nabavi

Keyword(s):

Oxidative Stress ◽

Rat Brain ◽

Gallic Acid ◽

Sodium Fluoride ◽

Protective Role

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Sodium fluoride induces nephrotoxicity via oxidative stress-regulated mitochondrial SIRT3 signaling pathway

Scientific Reports ◽

10.1038/s41598-017-00796-3 ◽

2017 ◽

Vol 7 (1) ◽

Cited By ~ 30

Author(s):

Chao Song ◽

Beibei Fu ◽

Jingcheng Zhang ◽

Jiamin Zhao ◽

Mengke Yuan ◽

...

Keyword(s):

Oxidative Stress ◽

Signaling Pathway ◽

Sodium Fluoride

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Perbedaan Kadar Glutation (GSH) Hepar Tikus Putih Jantan (Rattus norvegicus) yang diinduksi Parasetamol Dosis Toksik dengan Pemberian Ekstrak Daun Kelor (Moringa oleifera)

Jurnal Ilmiah Universitas Batanghari Jambi ◽

10.33087/jiubj.v20i1.881 ◽

2020 ◽

Vol 20 (1) ◽

pp. 247

Author(s):

Nur Insani ◽

H.M.T Kamaluddin ◽

Swanny Swanny

Keyword(s):

Oxidative Stress ◽

Treatment Group ◽

Leaf Extract ◽

The Body ◽

Control Group ◽

Bb Rat ◽

Toxic Dose ◽

Experimental Animals ◽

Significant Difference ◽

Group Ii

Glutathione (GSH) transferase deficiency due to paracetamol exposure causes further oxidative stress to liver necrosis. To reduce oxidative stress that can cause damage to the liver of the body requires antioxidants. One plant to treat liver disease is the kelor leaf (because it has an active flavonoid material also has antioxidant activity). This study was conducted to determine the difference of glutathione hepar levels of male white rat induced paracetamol toxic dose by giving kelor leaf extract. The type of research is experimental laboratory in vivo with rancagan randomized post test only control group design. With the stages as follows 1.Leaf Extract Kelor with Ethanol 96%, 2.Perpeteration of experimental animals, 3.Treatment of experimental animals by giving extract of 3-dose of kelor leaf that is KP I 250 mg / 200 gr BB rat, KP II 500 mg / 200 gr BB rat, KP III 1000 mg / 200 gr BB rat for 14 days combined with paracetamol dose 2 gr / 200 gr BB rat compared with the negative control group (group given only paracetamol dose 2 gr / 200 gr BB rat) and control group positif only fed regular feed for 14 days). The result showed that there was a significant difference mean of GSH levels between all treatment groups obtained p = 0,000 (p <α) p values smaller than 0.05. There was the highest increase of GSH in treatment group II (142,7525 μmol / mg) and lowest dose of GSH in positive control group (57,1812 μmol / mg), dose paracetamol toxic dosage and kelor leaf extract 500 mg / gr BB rat can increase GSH hepar p = 0,000 (p <α) p less than 0 , 05. The conclusion of the test results showed that giving of kelor leaf extract at dose of treatment group II can increase GSH hepar level significantly

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Protective Role of Gallic Acid Isolated from Peltiphyllum Peltatum Against Sodium Fluoride-Induced Oxidative Stress in Rat's Heart

Letters in Drug Design & Discovery ◽

10.2174/157018013804999690 ◽

2013 ◽

Vol 10 (3) ◽

pp. 277-282

Author(s):

Solomon Habtemariam ◽

Antoni Sureda ◽

Akbar Hajizadeh Moghaddam ◽

Seyed Fazel Nabavi ◽

Seyed Mohammad Nabavi ◽

...

Keyword(s):

Oxidative Stress ◽

Gallic Acid ◽

Sodium Fluoride ◽

Protective Role

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Effect of packing on changes in erythrocyte osmotic fragility and malondialdehyde concentration in donkeys administered with ascorbic acid

Onderstepoort Journal of Veterinary Research ◽

10.4102/ojvr.v79i1.413 ◽

2012 ◽

Vol 79 (1) ◽

Cited By ~ 3

Author(s):

Folashade Olaifa ◽

Joseph O. Ayo ◽

Suleiman F. Ambali ◽

Peter I. Rekwot

Keyword(s):

Oxidative Stress ◽

Ascorbic Acid ◽

Osmotic Fragility ◽

Blood Samples ◽

Experimental Animals ◽

Erythrocyte Osmotic Fragility ◽

Nacl Concentration ◽

Experimental Subjects ◽

Apparently Healthy

Experiments were performed with the aim of investigating the effect of packing on erythrocyte osmotic fragility (EOF) and malondialdehyde (MDA) concentration in donkeys, and the effect of ascorbic acid (AA). Twelve apparently healthy donkeys raised under the traditional extensive system served as experimental subjects. Six donkeys administered orally with AA (200 mg/kg) and subjected to packing were used as experimental animals, whilst six others not administered with AA served as controls. Blood samples were collected pre- and post-packing from all the donkeys for the determination of MDA and EOF. At 0.3% Sodium Chloride (NaCl) concentration, the percentage haemolysis was 93.69% ± 2.21% in the control donkeys and the value was significantly (P < 0.05) higher than the value of 71.31% ± 8.33%, recorded in the experimental donkeys. The post-packing MDA concentration obtained in the control donkeys was 39.62 µmol ± 4.16 µmol, and was not significantly different (P > 0.05) from the value of 35.97 µmol ± 2.88 µmol recorded in the experimental donkeys. In conclusion, the increase in haemolysis obtained in the donkeys suggested that packing induced oxidative stress, which was ameliorated by AA administration.

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