Comparative study on spawning success of paradise fish (Macropodus opercularis) using synthetic hormone and natural simulation

2020 ◽  
Vol 35 (1) ◽  
pp. 83-89
Author(s):  
P.C. Onuoha ◽  
K.O. Elezuo ◽  
E.G. Okeke

Synthetic hormones are widely used for induced breeding with significant successes. This study compared spawning successes in paradise fish (Macropodus opercularis) using synthetic hormone (Ovaprim) and natural simulation in glass tanks (0.3m x 0.6m). The experiment was conducted in two parts using two males and two females brood fish. The weights of the fish were between 4.0 and 5.0g for the males and 5.0 and 6.0g for the females. For the induced spawning, Ovaprim was injected intramuscularly according to the fish body weight after which they were left to spawn. The group on natural spawning were not induced by any hormone. Hatching of the eggs occurred within 48 hours at a temperature of 28.80C. Total number of eggs obtained by artificial and natural breeding were 430 and 322eggs respectively. Percentage fertilization in artificial breeding was 63.26% while 41.93% was recorded in natural breeding. Fry were raised on compounded diet for 6 weeks. Results showed that artificially bred fish had a higher mean weight gain (2.31 g) and survival rate (48.17%) than those from natural breeding having 2.10 g and 44.26% respectively. The results suggest that artificial breeding using synthetic hormone (Ovaprim) yielded better spawning success and survival rate than natural process. Keywords: Paradise fish, natural breeding, hormone, fertilization, survival

2019 ◽  
Vol 6 (2) ◽  
pp. 93
Author(s):  
Hadra Fi Ahlina ◽  
Yoyon Riono ◽  
Syaiful Ramadhan Harahap

AbstrakIkan betutu Oxyeleotris marmorata merupakan ikan lokal potensial menjadi komoditas budidaya. Performa pertumbuhan dan kelangsungan hidup dapat ditingkatkan dengan mengembangkan wadah budidaya. Tujuan penelitian adalah mengetahui jenis wadah budidaya yang optimal dalam mendukung pertumbuhan dan kelangsungan hidup. Perlakuan yang di uji adalah kolam tanah, kolam hapa dan kolam terpal berukuran 3 m x 2 m x 1 m. Ukuran benih yang digunakan 15±1,39 cm dengan bobot 250,04±1,70 g dengan padat tebar 25 ekor/kolam. Selama 120 hari masa pemeliharaan, pakan yang diberikan adalah ikan rucah sebanyak 30% dari bobot tubuh dengan frekwensi 2x sehari. Hasil penelitian menunjukkan perbedaan jenis wadah berpengaruh nyata terhadap pertambahan bobot dan laju pertumbuhan spesifik, namun tidak berpengaruh nyata terhadap kelangsungan hidup. Pertambahan bobot terbaik terdapat pada perlakuan jenis wadah kolam tanah sebesar 53,86±1,10 g dengan laju pertumbuhan spesifik 0,45±0,010 %bobot tubuh/hari dan kelangsungan hidup 85,33±6,11%. Kualitas air pada seluruh wadah pemeliharaan masih mendukung pertumbuhan dan kelangsungan hidup. Hasil penelitian ini dapat dijadikan informasi dasar dalam pemilihan wadah budidaya yang efektif dan efisien dalam pengembangan budidaya ikan betutu secara optimal.Kata kunci: ikan betutu; pertumbuhan; kelangsungan hidup; wadahAbstractSand goby (Oxyeleotris marmorata. Blkr) is a potential fish species for aquaculture in Indonesia. However, the growth and survival rate performance can be improved by developing cultivation containers. The research objective was to determine the optimal type of cultivation container to produce high growth and survival rate. The ponds treatments used in this experiment were soil pond, hapa pond and tarpaulin pond with measuring 3 m x 2 m x 1 m. The initial fish length average was 15±1.39 cm, with the initial body weight average of 250.04±1.70 g with the stocking density of 25 individual/pond. During 120 days of the rearing period, the fish were fed with trash fish with a proportion of 30% of body weight with the frequency of feeding 2x a day. The results showed differences in the type of container significantly affected weight gain and specific growth rates but did not significantly affect survival rate. The best weight gain was found in the treatment of soil pond containers at 53.86 ± 1.10 g with a specific growth rate of 0.45 ± 0.010% body weight/day and survival rate of 85.33 ± 6.11%. Water quality in all containers still supports growth and survival rate. The results of this study can be used as necessary information in the selection of effective and efficient aquaculture containers to produce optimal sand goby culture.Keywords: sand goby; growth; survival rate; container


2015 ◽  
Vol 2 (1) ◽  
pp. 169-175
Author(s):  
Gias Uddin Ahmed ◽  
Habiba Aktar ◽  
Sudristi Chakma ◽  
Neaz Al Hasan ◽  
Mohammad Shamsuddin

Correction: On 29th April 2015 the page numbers of this article were changed from 167-173 to 169-175An investigation was conducted to determine the comparative growth study of supermale tilapia and monosex tilapia in earthen mini ponds from May-July 2012. Four treatments were considered having two replicates. For supermale tilapia treatments were named as ST1 and ST2 and for monosex tilapia were MT1 and MT2. All the fish were of same age group having mean body weight of 1.4 g. Feeding frequency in all the treatments were two times a day. Fish were fed diet at a rate of 30% of their body weight for the first thirty days that was gradually reduced to 15% for the next thirty days and 5% till the termination of the experiment. Final weight, weight gain, average daily weight gain, % weight gain and production of supermale tilapia were significantly (p<0.05) higher than those of monosex tilapia. But SGR (% day), FCR and survival rate of supermale tilapia were not significantly (p>0.05) varied. However, the result of the present study showed that the best weight gain of 124.85 g was observed in ST1 after 90 days culture period. Average weight gain (g) were 1.39, 1.16, 1.14 and 1.05, SGR (per day) were 2.17, 2.09, 2.08 and 2.04%, FCR were 2.98, 2.65, 2.84 and 2.57, survival rate were 96, 94.50, 95 and 91% and fish production were 5053.92, 8926.10, 4108.07 and 7821.41 kg/ha in ST1, ST2, MT1 and MT2 respectively. The present research findings suggested that supermale tilapia has significantly high growth potential compare to monosex tilapia under mini ponds culture condition.Res. Agric., Livest. Fish.2(1): 169-175, April 2015


Author(s):  
N Islam ◽  
MF Islam ◽  
MS Islam ◽  
MI Miah

An experiment on induced breeding of menoda catfish, Hemibagrus menoda was conducted during the period from May 2017 to July 2018 by using commercial Ovupin hormone (GnRHa, Gonadotropin Releasing Hormone Analogues) to attain its optimum dose. The study consists of three treatments, each with three replications. The objective of the experiment was to find out the effective dose for induced breeding. The breeding parameters were determined in terms of ovulation, fertilization, hatching, and survival rate. A total of 36 males and 18 females lived brood fish were kept in the ratio of 2♂:1♀ for breeding purpose. The commercial Ovupin hormone were injected at the doses of 4 (T1), 6 (T2), 7 (T3) mL Ovupin·kg-1body weight for female and 1.5(T1), 2 (T2), 3(T3) mL Ovupin·kg-1 body weight for male in a triplicate replication. In this study, it was found that treatment T2 demonstrated the best spawning performance for both male and female and were statistically significant (p<0.05). The investigation indicated that the dose of Ovupin hormone 6mL Ovupin·kg-1body weight for female and 2 mL Ovupin·kg-1 body weight for male determines the highest spawning performance of menoda catfish which should be recommended for high quality eggs and larvae. J. Biodivers. Conserv. Bioresour. Manag. 2020, 6(2): 11-18


2019 ◽  
Vol 7 (2) ◽  
Author(s):  
D K Purnamasari ◽  
Erwan ◽  
K G Wiryawan ◽  
Nurmaya ◽  
Syamsuhaidi

Penelitian ini bertujuan untuk mengetahui pengaruh media tumbuh yang berasal dari pakan berbeda yang ditambahkan ampas tahu terhadap pertumbuhan dan produksi larva dan survival rate dari pupa. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan 3 perlakuan 9 ulangan dan masing-masing ditumbuhkan larva sebanyak 100 g (umur 45 hari). Adapun perlakuan yang diterapkan adalah perlakuan A= dedak padi 500 g + ampas tahu 800 g; perlakuan B= jagung giling halus 500 g + ampas tahu 800 g; perlakuan C= pakan komplit 500 g + ampas tahu 800 g. Variabel yang diamati yaitu konsumsi pakan, panjang badan, pertambahan bobot badan (PBB), konversi pakan, dan survival rate. Hasil penelitian menunjukkan bahwa konsumsi pakan, panjang badan, pertambahan bobot badan secara statistik berbeda secara nyata (P<0,05) dengan perlakuan C memiliki nilai tertinggi dibandingkan perlakuan lainnya. Konversi pakan berbeda secara nyata (P<0,05) dengan perlakuan A memiliki nilai konversi terendah. Survival rate secara statistik berbeda secara nyata (P<0,05) dengan bahwa perlakuan B memiliki nilai tertinggi. Kesimpulan dari penelitian ini bahwa penggunaan media pakan komplit + ampas tahu adalah yang terbaik untuk pertumbuhan dan perkembangan larva. Kata kunci: Pertumbuhan, Survival Rate, Larva, Tenebrio Molitor, Media Pakan This study aims was to determine the effect of growing media derived from different feeds added to tofu waste on growth and larval production and the survival rate of pupae. The study used a Completely Randomized Design (CRD) with 3 treatments 9 replications and each of them grew as many as 100 g (age 45 days). The treatment conducted treatment A (rice bran 500 g + tofu waste 800 g); treatment B (fine corn 500 g + tofu waste 800 g); and treatment C ( complete feed 500 g + tofu waste 800 g). The variables observed were, feed consumption, body length, body weight gain, feed conversion, and survival rate. The variables observed were, feed consumption, body length, body weight gain, feed conversion, and survival rate. The results of this study was showed that feed consumption, body length, body weight gain were statistically significantly different (P <0.05) with treatment C had the highest value compared to other treatments. Feed conversion was significantly different (P <0.05) with treatment A had the lowest conversion value. The survival rate was statistically significantly different (P <0.05) with that treatment B had the highest value. The conclusion of this study is that the use of complete feed media + tofu waste is the best for the growth and development of larvae. Keywords: growth, survival rate, larvae, Tenebrio molitor, feed media_________________________________________________________


Author(s):  
E. Grinberg

The article contains and summarizes typical biotechnical errors and violations that are made on a daily and routine basis by fish breeders. Deviations from the biological basis of fish breeding are also shown and possible consequences of such errors or violations are predicted. Their correction, prevention or minimization of consequences, will significantly improve the quality of sexual products and offspring from producers, increase the linear and weight gain of juveniles during rearing, improve the feed payment coefficient, reduce the time of rearing, improve the epizootic situation by developing immunity in fry and mobilizing other protective reactions of their body, significantly reduce waste at all stages of the production process, increasing the survival rate of fry by 10–15 %.


2012 ◽  
Vol 4 (1) ◽  
Author(s):  
Muhammad Marzuqi ◽  
Ni Wayan Widya Astuti ◽  
Ketut Suwirya

<p>The grouper fish culture was developed after its succesful seed production in hatchery well known. In grow-out culture grouper, the protein requirement and feeding rate have to know well in order to understand the effectiveness on feed utilization . The experiment was designed by factorial design with the first factor as 3 dietary protein (36%, 42%, 48%) and the second factor as 3 feeding rate ( 1,5%, 2,0%, 2,5%). Ten of juvenile tiger grouper (102, 51-102, 73 g of body weight) were stocked in 27 polycarbonate tanks with 400 l volume each. Each tank was equiped with aeration and flow-through water system. Fish was fed three time per day i.e. 08:00, 11:30 and 15:30 Central Indonesia Standard Time for 16 weeks. Every week was measured body weight, survival rate, and feed quantity. The parameter used in this experiment were weight gain, spesific growth rate, feed efficiency and survival rate. The results of the experiment showed that the combination of protein level (36%, 42%, 48%) with feed ratio (1.5%, 2.0%, 2.5%) gave response on growth, weight gain and survival rate. Combination dietary protein level of 48% and feed ratio 1.5% gave higher weight gain and survival rate with values of 104.05 ± 5.89% and 96.67 ± 5.57 %, respectively.</p><p>Keywords: Dietary protein and feeding rate , growth, tiger grouper (Epinephelus fuscoguttatus)</p>


Vaccines ◽  
2020 ◽  
Vol 8 (3) ◽  
pp. 452
Author(s):  
Xingju Song ◽  
Xu Yang ◽  
Taotao Zhang ◽  
Jing Liu ◽  
Qun Liu

Eimeria tenella (E. tenella) is a highly pathogenic and prevalent species of Eimeria that infects chickens, and it causes a considerable disease burden worldwide. The secreted proteins and surface antigens of E. tenella at the sporozoite stage play an essential role in the host–parasite interaction, which involves attachment and invasion, and these interactions are considered vaccine candidates based on the strategy of cutting off the invasion pathway to interrupt infection. We selected two highly expressed surface antigens (SAGs; Et-SAG13 and Et-SAG) and two highly expressed secreted antigens (rhoptry kinases Eten5-A, Et-ROPK-Eten5-A and dense granule 12, Et-GRA12) at the sporozoite stage. Et-ROPK-Eten5-A and Et-GRA12 were two unexplored proteins. Et-ROPK-Eten5-A was an E. tenella-specific rhoptry (ROP) protein and distributed in the apical pole of sporozoites and merozoites. Et-GRA12 was scattered in granular form at the sporozoite stage. To evaluate the potential of rEt-ROPK-Eten5-A, rEt-GRA12, rEt-SAG13 and rEt-SAG proteins as a coccidiosis vaccine, the protective efficacy was examined based on survival rate, lesion score, body weight gain, relative body weight gain and oocyst output. The survival rate was significantly improved in rEt-ROPK-Eten5-A (100%) and rEt-GRA12 (100%) immune chickens compared to the challenged control group (40%). The average body weight gains of rEt-ROPK-Eten5-A, rEt-GRA12, rEt-SAG13 and rEt-SAG immunized chickens were significantly higher than those of unimmunized chickens. The mean lesion score and oocyst output of the rEt-ROPK-Eten5-A immunized chickens were significantly reduced compared to unimmunized challenged chickens. These results suggest that the rEt-ROPK-Eten5-A protein effectively triggered protection against E. tenella in chickens and provides a useful foundation for future work developing anticoccidial vaccines.


Author(s):  
Muhammad Marzuqi ◽  
Ni Wayan Widya Astuti ◽  
Ketut Suwirya

The grouper fish culture was developed after its succesful seed production in hatchery well known. In grow-out culture grouper, the protein requirement and feeding rate have to know well in order to understand the effectiveness on feed utilization . The experiment was designed by factorial design with the first factor as 3 dietary protein (36%, 42%, 48%) and the second factor as 3 feeding rate ( 1,5%, 2,0%, 2,5%). Ten of juvenile tiger grouper (102, 51-102, 73 g of body weight) were stocked in 27 polycarbonate tanks with 400 l volume each. Each tank was equiped with aeration and flow-through water system. Fish was fed three time per day i.e. 08:00, 11:30 and 15:30 Central Indonesia Standard Time for 16 weeks. Every week was measured body weight, survival rate, and feed quantity. The parameter used in this experiment were weight gain, spesific growth rate, feed efficiency and survival rate. The results of the experiment showed that the combination of protein level (36%, 42%, 48%) with feed ratio (1.5%, 2.0%, 2.5%) gave response on growth, weight gain and survival rate. Combination dietary protein level of 48% and feed ratio 1.5% gave higher weight gain and survival rate with values of 104.05 ± 5.89% and 96.67 ± 5.57 %, respectively.Keywords: Dietary protein and feeding rate , growth, tiger grouper (Epinephelus fuscoguttatus)


2017 ◽  
Vol 53 ◽  
pp. 115-119
Author(s):  
A. V. Dymchuk ◽  
O. I. Lyubynskyy

The studies of the dynamics results of body weight, absolute and daily average, relative increases averaging bulls of Ukrainian black-and-white and Ukrainian red-and-white dairy cattle. Researches are conducted in the conditions of POSP «Napadivske» Kalyinivka district of the Vinnytsya area after materials of primary pedigree account. In an economy use the whole-year of the same type feeding. In the structure of ration 40% make the concentrated sterns and also high-quality silage and soilage. Average daily increases of bulls for period of age to realization for a slaughter made over 1000 grammes. The dynamics of growth of living mass, absolute, average daily and relative increases of bulls, is analysed Ukrainian black-and-white and Ukrainian red-and-white dairy cattle. Living mass and weight gain researches of age to 12-monthly age with an interval in 3 months after the generally accepted methods. Materials of researches are worked out the methods of mathematical statistics with the use of programmatic package of Statistica 8.0. Living mass of bulls of the second group at birth made 36,3 kg, that anymore on 0,8 kg by comparison to new-born bulls of the first group. In all next periods living mass of animals of the second group was greater as compared to the animals of the same age of the first. In 3 months advantage made 1,7 kg, in 6 months – 4,6 kg (P>0,95), in 9 months – 8,8 kg (P>0,95), in 12 months – 12,5 kg (P>0,99). Difference of absolute increases of bulls of the second group above the bulls of the same age of the first of age to to 3-monthly age made 0,8 kg in behalf of animals of the second group. In a period 3-6 months a difference is after this index between the first and second groups made 2,9 kg in behalf of the last. In periods 6-9 and reliable advantage of animals of the second group is 9-12 months set above the bulls of the same age of the first on 4,1 kg (P>0,95) and 3,7 kg (P>0,95) accordingly. In a period of age to 18-monthly age absolute increases of bulls of the second group were greater by comparison to bulls first on 12,5 kg (R>0,99). Average daily increases of bulls of the second group of age to 3-monthly age made 763,0 grammes, that anymore on 8,9 grammes by comparison to bulls of the first group. In periods 3-6 months the average daily increases of animals of the second group prevailed the bulls of the same age of the first on 32,6 grammes. In a period 6-9 months the average daily increases of animals of the second group were made by 1045,9 grammes, that for certain anymore on 45,9 grammes (P>0,95) by comparison to the bulls of the same age of the first. Maximal increases of bulls were in a period 9-12 months and made 1210,4 grammes in the animals of the second group and 1168,9 grammes – for the bulls of the same age of the first, that for certain anymore on 41,5 grammes (P>0,95). In a period of age to 12-monthly age the average daily increases of bulls of the second group were made by 970,6 grammes, that was for certain anymore on 31,8 grammes (R>0,99) by comparison to the bulls of the same age of the first group. Relative increases of bulls of both groups were high. From birth to 3-monthly age greater increases had bulls of the first group – 97,8%, and in periods 3-6, 6-9 and 9-12 months greater were increases of bulls of the same age of the second group – 56,4; 40,1 and 32,4% accordingly. For all probed periods of reliable advantage it is not discovered between the compared groups. It is set researches, that at the identical terms of feeding and maintenance more rapid bulls grow Ukrainian red-and-white dairy cattle. In all age-old periods they prevailed the bulls of the same age of the Ukrainian black-and-white dairy cattle, in a that number, beginning from 9-monthly age, advantage was reliable (P>0,95–0,99). Average daily increases of bulls of two breeds were the least in a period of age to to 3-monthly age – 754,1-763,0 grammes, and most in a period 9-12 months – 1168,9–1210,4 grammes.


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