scholarly journals Nutritional composition, antioxidant assay and α-glucosidase inhibitory flavonoids from the fruits of Carissa edulis Vahl (Apocynaceae)

2021 ◽  
Vol 18 (2) ◽  
pp. 122-132
Author(s):  
Olalekan Stephen Ojerinde ◽  
Dalen Dafam Gwatau ◽  
Kakjing Dadul Falang ◽  
Patricia O. Odumosu ◽  
Jacob Adegboyega Kolawole
Keyword(s):  
Nutritive Value ◽  
Spectroscopic Analysis ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Proximate Analysis ◽  
Nutritional Composition ◽  
Total Phenolic ◽  
Meoh Extract ◽  
Microbial Infections ◽  
Total Flavonoids Content

Carissa edulis Vahl (Apocynaceae) is a plant whose leaves, roots and fruits are traditionally employed for the treatment of malaria, microbial infections, headache, cough, fever and ulcer among many others. Fresh fruits of Carissa edulis were crushed and extracted with 70 % methanol for 72 hours to obtain MeOH extract. Proximate analysis as well as mineral composition of the fresh fruits were determined. Total phenolic content (TPC), total flavonoids content (TFC) and antioxidant activity (DPPH, FRAP & Ferrous chelating) of the MeOH extract were determined, while the α-glucosidase inhibitory activity of the extract was conducted using bioautographic assay. The structure of compounds isolated were elucidated by 1D NMR spectroscopic analysis and high-resolution mass spectrometric (HRESIMS) data. The results showed that the nutritive value of the fresh Carissa edulis is 43.43 kcal/100 g, while the extract demonstrated good antioxidant activities (DPPH, IC50 = 87.98 μg/ml; FRAP, EC50 = 464.33 μg/ml & Ferrous chelating, EC50 = 294.55 μg/ml). Further study of the MeOH extract of the fruit led to the isolation of rhamnetin-3-β-D-glucopyranoside, peonidin-3-rutinoside and malvidin-3-O-β-D-(6”-acetylglucoside). These results suggest that the fruits of Carissa edulis are nutritious and possess potential antidiabetic effect which corroborated the ethnomedical uses of the fruits. Keywords: Carissa edulis; α-Glucosidase; Anthocyanins; Nutritional composition; Antioxidant properties

scholarly journals Comparison of selected local honey with Manuka honey based on their nutritional and antioxidant properties

Food Research ◽  
2020 ◽  
Vol 4 (S1) ◽  
pp. 205-213
Author(s):  
T.K. Zae ◽  
A. Azlan ◽  
A. Abu Bakar Sajak ◽  
E.K. Hock ◽  
N.L. Chin ◽  
...  
Keyword(s):  
Phenolic Content ◽  
Antioxidant Activities ◽  
Energy Content ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Proximate Analysis ◽  
Total Phenolic ◽  
Acid Oxidation ◽  
Manuka Honey ◽  
Sugar Analysis

This study aimed to determine and compare the proximate composition, sugars content and antioxidant properties of selected Malaysian raw honey (Tualang, Gelam, Kelulut, Wild, and Pineapple) with Manuka honey. Proximate analysis (energy, carbohydrate, protein, fat, ash, moisture and total dietary fibre), sugar analysis (fructose, sucrose, glucose and maltose), total phenolic content (TPC) and antioxidant analysis (DPPH radical scavenging activity and linoleic acid oxidation assay) were conducted on the honey samples. The proximate analysis and sugar analysis results for all honeys were within Codex Alimentarius Commission guidelines. In general, Malaysian honeys exhibited lower level of carbohydrates (80.27% to 82.32%), energy (324.18 kcal/100 g to 331.20 kcal/100 g), ash (0.17% to 0.28%) and protein (0.24% to 0.26%, except for Gelam 0.45%) but higher in moisture (17.07% to 19.08%) and fat (0.10% to 0.36%) contents compared to Manuka honey. No significance differences (P>0.05) was detected in total sugar among the honey samples. Overall, among the selected honeys, Kelulut honey has the highest energy content, lowest moisture level, fat content and fructose level. Meanwhile, Wild honey showed the highest phenolic content and antioxidant activities.

scholarly journals Effect of particle size on phytochemical composition and antioxidant properties of Sargassum cristaefolium ethanol extract

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
E. S. Prasedya ◽  
A. Frediansyah ◽  
N. W. R. Martyasari ◽  
B. K. Ilhami ◽  
A. S. Abidin ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Particle Size ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Particle Sizes ◽  
Epicatechin Gallate ◽  
Particle Size Reduction ◽  
Phytochemical Composition

AbstractSample particle size is an important parameter in the solid–liquid extraction system of natural products for obtaining their bioactive compounds. This study evaluates the effect of sample particle size on the phytochemical composition and antioxidant activity of brown macroalgae Sargassum cristaefolium. The crude ethanol extract was extracted from dried powders of S.cristeafolium with various particle sizes (> 4000 µm, > 250 µm, > 125 µm, > 45 µm, and < 45 µm). The ethanolic extracts of S.cristaefolium were analysed for Total Phenolic Content (TPC), Total Flavonoid Content (TFC), phenolic compound concentration and antioxidant activities. The extract yield and phytochemical composition were more abundant in smaller particle sizes. Furthermore, the TPC (14.19 ± 2.08 mg GAE/g extract to 43.27 ± 2.56 mg GAE/g extract) and TFC (9.6 ± 1.8 mg QE/g extract to 70.27 ± 3.59 mg QE/g extract) values also significantly increased as particle sizes decreased. In addition, phenolic compounds epicatechin (EC), epicatechin gallate (ECG), epigallocatechin (EGC), and Epigallocatechin gallate (EGCG) concentration were frequently increased in samples of smaller particle sizes based on two-way ANOVA and Tukey’s multiple comparison analysis. These results correlate with the significantly stronger antioxidant activity in samples with smaller particle sizes. The smallest particle size (< 45 µm) demonstrated the strongest antioxidant activity based on DPPH, ABTS, hydroxyl assay and FRAP. In addition, ramp function graph evaluates the desired particle size for maximum phytochemical composition and antioxidant activity is 44 µm. In conclusion, current results show the importance of particle size reduction of macroalgae samples to increase the effectivity of its biological activity.

scholarly journals Nutritional Composition,α-Glucosidase Inhibitory and Antioxidant Activities ofOphiopogon japonicusTubers

2015 ◽  
Vol 2015 ◽  
pp. 1-7
Author(s):  
Yancui Wang ◽  
Feng Liu ◽  
Zongsuo Liang
Keyword(s):  
Amino Acids ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Nutritional Composition ◽  
Total Phenolic ◽  
Ophiopogon Japonicus ◽  
Nutritional Compounds ◽  
Chloroform Methanol ◽  
Phenolic And Flavonoid

Ophiopogon japonicustubers have been widely used as food and traditional Chinese medicine in China. However, their nutritional composition has not been fully reported yet. This study aimed to analyze the nutritional composition ofO. japonicustubers. Theα-glucosidase inhibitory and antioxidant activities of the extracts obtained fromO. japonicustubers were also evaluated byin vitroassays. The results indicated thatO. japonicustubers are rich in carbohydrates, proteins, minerals, and amino acids. Among four extracts, the n-butanol fraction (nBF) and chloroform/methanol extract (CME) ofO. japonicustubers had high amounts of total phenolic and flavonoid contents and exhibited goodα-glucosidase inhibitory and antioxidant activities. Theα-glucosidase inhibition of nBF was higher than acarbose. Overall,O. japonicustubers are full of nutritional compounds and have goodα-glucosidase inhibitory and antioxidant activities.

scholarly journals Evaluation of Antioxidant Properties ofPhaulopsis fascisepalaC.B.Cl. (Acanthaceae)

2009 ◽  
Vol 6 (2) ◽  
pp. 227-231 ◽  
Author(s):  
S. A. Adesegun ◽  
A. Fajana ◽  
C. I. Orabueze ◽  
H. A. B. Coker
Keyword(s):  
Ascorbic Acid ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Chelating Ability

The antioxidant activities of crude extract ofPhaulopsis fascisepalaleaf were evaluated and compared with α-tocopherol and BHT as synthetic antioxidants and ascorbic acid as natural-based antioxidant.In vitro, we studied its antioxidative activities, radical-scavenging effects, Fe2+-chelating ability and reducing power. The total phenolic content was determined and expressed in gallic acid equivalent. The extract showed variable activities in all of thesein vitrotests. The antioxidant effect ofP. fascisepalawas strongly dose dependent, increased with increasing leaf extract dose and then leveled off with further increase in extract dose. Compared to other antioxidants used in the study, α-Tocopherol, ascorbic acid and BHT,P. fascisepalaleaf extract showed less scavenging effect on α,α,-diphenyl-β-picrylhydrazyl (DPPH) radical and less reducing power on Fe3+/ferricyanide complex but better Fe2+-chelating ability. These results revealed thein vitroantioxidant activity ofP.fascisepala.Further investigations are necessary to verify these activitiesin vivo.

scholarly journals ANTIOXIDANT PROFILE AND PHYTOCHEMICAL CONTENT OF DIFFERENT PARTS OF SUPER RED DRAGON FRUIT (HYLOCEREUS COSTARICENSIS) COLLECTED FROM WEST JAVA-INDONESIA

2017 ◽  
Vol 10 (12) ◽  
pp. 290 ◽  
Author(s):  
Irda Fidrianny ◽  
Nadia Ilham ◽  
Rika Hartati
Keyword(s):  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Phytochemical Content ◽  
Dragon Fruit ◽  
Dpph Method ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging ◽  
Different Parts

Objectives: The goals of this research were to observe antioxidant properties from different parts of super red dragon fruit (Hylocereus costaricensis) using two antioxidant testing methods which were 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS).Methods: Antioxidant activities were determined using DPPH and ABTS assays, total phenolic content (TPC) using Folin–Ciocalteu reagent, flavonoid content by Chang’s method.Results: Inhibitory concentration 50% (IC50) of DPPH scavenging activity of all of the extracts in the range of 2.69 μg/ml was −94.17 μg/ml. The ethyl acetate peel extract of super red dragon fruit expressed the highest TPC (4.56 g GAE/100 g) and the highest total flavonoid content (12.63 g QE/100 g). TPC in flesh extract of super red dragon fruit had a negative and significant correlation with their IC50 of ABTS. The IC50 of DPPH and IC50 of ABTS of flesh extract of super red dragon fruit showed positive and significant correlation.Conclusion: All different parts extracts of super red dragon fruit (except n-hexane flesh extract) were categorized as a very strong antioxidant by DPPH method. Phenolic compounds in flesh extract of super red dragon fruit were the major contributor in antioxidant activities by ABTS method. DPPH and ABTS showed linear results in antioxidant activities of super red dragon fruit flesh extract.

scholarly journals Comparative Study on the Nutritional and Antioxidant Components of Fruit Parts of Citrullus lanatus

2020 ◽  
pp. 39-51
Author(s):  
Stanley Kanayochukwu Nnenne ◽  
Kingsley Ikechukwu Ubaoji ◽  
Uchechukwu Chibuzo Ogbodo ◽  
Victor Henry Azubuike Enemor ◽  
Adebayo Afees Oladejo
Keyword(s):  
Citrullus Lanatus ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Proximate Analysis ◽  
Nutritional Composition ◽  
The People ◽  
Dpph Scavenging Activity ◽  
Student’S T ◽  
Dpph Scavenging ◽  
Student’S T Test

Aim: Citrullus lanatus is a fruit widely consumed for its pulp though incompletely, as its other parts (seed and rind) are discarded and may possibly offer bioactive compounds involved in ameliorating certain disease conditions. Hence, this study aimed at comparatively investigating the nutritional composition and antioxidant properties of the seed, pulp and rind of C. lanatus so as to inform the inclusion of these different parts into the fare of the people. Methodology: Standard analytical methods of Association of Official Analytical Chemists were used to determine proximate, phytochemical, vitamin, mineral and antioxidant aspects of the fruit parts. The results were analyzed using Student’s t-test at .05. Results: The proximate analysis showed a high moisture value in the pulp (93.34 +/- .82) followed by the rind (77.11 +/- 3.44) and the seed (10.00 +/- .48). High crude protein and fiber contents were both noted in the seed followed by the rind and pulp. Mineral determinations revealed potassium to be abundant in the rind (452.31mg/kg) than the seed (305.7mg/kg) and the pulp (100.5mg/kg), followed by calcium occurring more in the rind (292.61mg/kg) than in the pulp (257.21mg/kg) and seed (227.45mg/kg) and then manganese being the least concentrated among the minerals. The investigated phytochemical principles revealed the highest concentration of cardiac glycosides in the seed (14.82 +/- .66) than the rind (1.95 +/- 0.80) and pulp (1.10 +/- 0.17), followed by saponins occurring most in the rind (12.05 +/- 3.91) than the seed (10.17 +/- 0.63) and pulp (.13 +/- .01). The seed had the highest DPPH scavenging activity followed by the rind and pulp respectively. The seed also showed a higher reducing power and lipid peroxidation capacities than the other fruit parts suggesting a potent property for antioxidant activity in the fruit parts of C. lanatus. Conclusion: The findings imply that both seed and rind of C. lanatus can be good sources of nutritional, phytochemical and antioxidant components in addition to the pulp which is commonly consumed and these may be implicated in management of certain diseases with further evidential research.

scholarly journals UPLC-QTOF-MS/MS and GC-MS Characterization of Phytochemicals in Vegetable Juice Fermented Using Lactic Acid Bacteria from Kimchi and Their Antioxidant Potential

Antioxidants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1761
Author(s):  
Moeun Lee ◽  
Jung Hee Song ◽  
Eun Ji Choi ◽  
Ye-Rang Yun ◽  
Ki Won Lee ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Phenyllactic Acid ◽  
Antioxidant Power ◽  
Vegetable Juice ◽  
Daucus Carota L ◽  
Ferric Reducing Antioxidant Power

This study aims to investigate fermentative metabolites in probiotic vegetable juice from four crop varieties (Brassica oleracea var. capitata, B. oleracea var. italica, Daucus carota L., and Beta vulgaris) and their antioxidant properties. Vegetable juice was inoculated with two lactic acid bacteria (LAB) (Companilactobacillus allii WiKim39 and Lactococcus lactis WiKim0124) isolated from kimchi and their properties were evaluated using untargeted UPLC-QTOF-MS/MS and GC-MS. The samples were also evaluated for radical (DPPH• and OH•) scavenging activities, lipid peroxidation, and ferric-reducing antioxidant power. The fermented vegetable juices exhibited high antioxidant activities and increased amounts of total phenolic compounds. Fifteen compounds and thirty-two volatiles were identified using UPLC-QTOF-MS/MS and GC-MS, respectively. LAB fermentation significantly increased the contents of d-leucic acid, indole-3-lactic acid, 3-phenyllactic acid, pyroglutamic acid, γ-aminobutyric acid, and gluconic acid. These six metabolites showed a positive correlation with antioxidant properties. Thus, vegetable juices fermented with WiKim39 and WiKim0124 can be considered as novel bioactive health-promoting sources.

Total Phenolic, Antioxidant Activities and Flavonoid Contents of Herbal Syrups

2019 ◽  
Vol 800 ◽  
pp. 60-64
Author(s):  
Fredijs Dimiņš ◽  
Ingrīda Augšpole
Keyword(s):  
Antiradical Activity ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Scavenging Activity ◽  
Total Phenols ◽  
Total Phenolic Compounds ◽  
Trolox Equivalent

The aim of the study was to characterize antioxidative properties and antiradical activity of the herbal syrups collection in Latvia. Spectrophotometry was used to characterize antioxidant properties of herbal syrups. Antiradical activity, total phenols and content of flavonoids were determined spectrophotometrically. The antiradical scavenging activity was measured by the DPPH (2.2-diphenyl-1-picrylhydrazyl) reaction. The herbal syrups antiradical scavenging activity was estimated by the amount of DPPH of reagent using in reaction with a sample of vegetable syrup (in percent) and by Trolox equivalent. The total phenolic compounds were determined by using the Folin – Ciocalteu method. The total phenolic content was expressed in mg of the gallic acid equivalents. The content of flavonoids was determined by using reaction between the AlCl3 and flavonoids. Antiradical scavenging activity by Trolox equivalent of the herbal syrups was found from 81 to be 130 mg 100 g-1 herbal syrups. The content of total phenols was found from 22 to be 282 mg GAE 100 g-1, but content of total flavonoids by quercetin equivalent was found from 1 to 44 mg QE 100 g-1. In case of increased disease problems, syrups with increased antioxidant content should be used. However, the specificity of each disease should be taken into account as well as the content of certain compounds in the plants.

Antioxidant, hypoglycemic and anti-hypertensive properties of extracts derived from peel, fruit and kernel of Salak

2020 ◽  
Vol 122 (10) ◽  
pp. 3029-3038
Author(s):  
Seok Shin Tan ◽  
Seok Tyug Tan ◽  
Chin Xuan Tan
Keyword(s):  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Alpha Amylase ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Total Phenolic ◽  
Distilled Water ◽  
Antioxidant Power ◽  
Content Type ◽  
Fruit Extracts ◽  
Significant Difference

PurposeSalak (Salacca zalacca) is an underutilised fruit. The bioactivities of this fruit have rarely been studied scientifically. Thus, the present study aimed to determine the antioxidant activity of extracts derived from the peel, fruit and kernel of the Salak fruit, as well as the hypoglycemic and anti-hypertensive properties of Salak peel extracts.Design/methodology/approachThe peel, fruit and kernel of the Salak were extracted using distilled water, methanol and ethanol. Antioxidant activities, angiotensin-converting enzyme (ACE) and alpha-amylase inhibition properties of the extracts were estimated via in vitro standard methods. Besides, the total phenolic content (TPC) and total flavonoid content (TFC) of the extracts were also determined in the present study. The antioxidant activities of different parts of Salak extracts were determined by ferric-reducing antioxidant power (FRAP) and Trolox equivalent antioxidant capacity (TEAC) methods. Percent of radical scavenging properties were calculated via DPPH assay. The hypoglycemic and anti-hypertensive properties of Salak peel were evaluated using alpha-amylase inhibition and ACE assays, respectively.FindingsFruit extracts of Salak in methanol were found to exhibit the highest TPC (10.27 ± 0.12 mg GAE/g), TFC (11.04 ± 0.89 mg CE/g) and antioxidant properties amongst all samples whereby the TPC and TFC were strongly correlated with antioxidant activities. On the other hand, distilled water extracted Salak kernel showed to have the lowest TPC (0.53 ± 0.05 mg GAE/g), TFC (0.37 ± 0.01 mg CE/g) and antioxidant properties amongst all the Salak extracts. Peel extracts exhibit comparable antioxidant activities with fruit extracts in the current findings. In addition, peel extracts indicated some extend of ACE and alpha-amylase inhibition activities regardless of the solvents used. Methanol and ethanol peel extracts indicated no significant difference (p < 0.05) ACE (98%) and alpha-amylase (90%) inhibition activities. However, distilled water extracted Salak peel showed significantly lower ACE and alpha-amylase inhibition in comparison to methanol and ethanol peel extracts.Originality/valueThe present findings suggested that the fruit of Salak exhibits the highest antioxidant properties, followed by the peel and lastly, the kernel, which shows the lowest antioxidant properties amongst all the samples. The results also indicated that the peel extracts have ACE and alpha-amylase inhibition activities.

scholarly journals A Comparative Study on Antioxidant Properties of Two Phyllanthus Species Growing in Bangladesh

2013 ◽  
Vol 11 (2) ◽  
pp. 191-197 ◽  
Author(s):  
Ishrat Nimmi ◽  
Ismet Ara Jahan ◽  
M Hemayet Hossain ◽  
Mohammad Burhan Uddin ◽  
M Sohel Rana ◽  
...  
Keyword(s):  
Comparative Study ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Test Methods ◽  
Total Phenolic ◽  
Solvent Volume

A comparative study of antioxidant properties on two varieties of Phyllanthus (Phyllanthus niruiri L. & Phyllanthus urinaria L. leaves) growing in Bangladesh was done in the present study. Five complimentary test methods namely DPPH free radical scavenging activity, reducing power assay, total antioxidant capacity, total phenolic and flavonoid contents determination were used for the evaluation of antioxidant properties of two species of Phyllanthus. In the present investigation for the evaluation of antioxidant activities the concentration is expressed by the ratio of crude sample per solvent volume instead of extract weight per solvent volume. Based on these concentrations the investigated P. niruri L. and P. urinaria L. leaves extracts exhibited significant results. At 2 mg/ml concentration DPPH radical scavenging capacity of the methanol extract of both the plants was found to show significant (>90%) activity which is comparable to Ascorbic acid and BHA. In case of reducing power tests for both the extract, the activity of P. urinaria is comparable to that of BHA and AA and that of P. niruiri is comparable to BHA. P. niruiri showed better antioxidant potential than that of P. urinaria based on the observed results of their corresponding methanol extract. DOI: http://dx.doi.org/10.3329/dujps.v11i2.14579 Dhaka Univ. J. Pharm. Sci. 11(2): 191-197, 2012 (December)

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