Development of a modified environment for oyster mushroom (Pleurotus ostreatus) production system for rural use in Southeast Nigeria

This study investigated a Pleurotus ostreatus production in Nsukka, Nigeria, using the locally available materials. A modified controlled environmental system capable of maintaining the micro elements (temperature and relative humidity) suitable for cultivation of oyster mushroom was developed. The system was tested using substrates of biomaterial origin (saw dust and rice bran) at different levels of treatments. The substrates used were fermented sawdust supplemented with rice bran at a spawning level of 0%, 5%, 10%, 15%, and 20%. The steps involved in the cultivation were composting, bagging, spawning, incubation and cropping the substrates. The experiment was laid out in a completely randomized design in five replications and results analysed at P < 0.05 significant difference. The biological efficiency was determined to establish the minimum suitable nutrient supplementation levels and the best yield of Pleurotus ostreatus. The nutritional compositions of the yield were analysed. The highest mean yield of 24.5 g of fresh mushrooms was observed with the substrate mixture T3 (15% rice bran and 85% saw dust) and lowest yield of 8.6 g observed in the T0 (without rice bran). The effects of temperature and relative humidity on growth of oyster mushroom were evaluated. The result of this study showed that small-scale, indoor oyster mushroom production appears to be economically feasible in Nsukka.

Download Full-text

Pengaruh penambahan serbuk jagung pada komposisi media tanam terhadap pertumbuhan miselium bibit F1 jamur tiram putih (Pleurotus ostreatus)

BIOMA Jurnal Ilmiah Biologi ◽

10.26877/bioma.v10i2.7429 ◽

2021 ◽

Vol 10 (2) ◽

pp. 158-170

Author(s):

Muhaeming Muhaeming ◽

Jamilah Jamilah ◽

Zulkarnaim Zulkarnaim

Keyword(s):

Experimental Study ◽

Analysis Of Variance ◽

Pleurotus Ostreatus ◽

Medium Composition ◽

Oyster Mushroom ◽

Control Treatment ◽

Mycelium Growth ◽

Randomized Design ◽

Significant Difference ◽

Completely Randomized Design

Penambahan nutrisi perlu dilakukan untuk meningkatkan produktivitas pertumbuhan jamur tiram putih (Pleurotus ostreatus). Salah satu bahan yang dapat digunakan adalah serbuk jagung. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan serbuk jagung pada komposisi media tanam terhadap pertumbuhan miselium bibit F1 jamur tiram putih (Pleurotus ostreatus) dan mengetahui konsentrasi serbuk jagung yang paling baik digunakan pada pertumbuhan miselium bibit F1 jamur tiram putih (Pleurotus ostreatus). Penelitian ini mengunakan Rancangan Acak Lengkap (RAL) 4 perlakuan dan 3 ulangan sehingga diperoleh 12 satuan percobaan; P0 (perlakuan kontrol), P1 (25%), P2 (50%), dan P3 (100%). Variabel yang diukur adalah persentase pertumbuhan miselium yang diamati pada hari ke-6, 12 dan 18 dan waktu penyebaran miselium Hari Setelah Inokulasi (HSI). Data hasil penelitian dianalisis menggunakan analysis of variance (ANOVA) dengan uji lanjut menggunakan uji Beda Nyata Terkecil (BNT). Hasil penelitian menunjukkan bahwa ada pengaruh pada pertumbuhan miselium bibit F1 jamur tiram putih (Pleurotus ostreatus) dengan penambahan serbuk jagung pada komposisi media tanam. Selain Itu, konsentrasi serbuk jagung yang memberikan hasil yang paling baik terhadap pertumbuhan miselium adalah P2 dengan konsentrasi 50%. Kata kunci : miselium F1; Pleurotus ostreatus; serbuk jagung ABSTRACTAdditional nutrition needs to be done to increase the productivity of oyster mushroom (Pleurotus ostreatus) growth. One of the materials that can be used is corn powder. This study aimed to determine the effect of corn powder on the planting medium composition towards mycelium growth of oyster mushroom F1 seeds (Pleurotus ostreatus) and to find the best concentration of corn powder to grow mycelium seeds F1 oyster mushroom (Pleurotus ostreatus). This research was an experimental study using a Completely Randomized Design (CRD) with 4 treatments namely; P0 (control treatment), P1 (25%), P2 (50%), and P3 (100%), and 3 repetitions so that obtained 12 experimental units. The variables measured were the mycelium growth percentage on days 6, 12, and 18, and the mycelium widespread time after the day of inoculation. The data were analyzed using ANOVA then continued on the Least Significant Difference (LSD) test. The results showed that the addition of corn powder on oyster mushroom growing media affected mycelium growth of the oyster mushroom F1 seeds (Pleurotus ostreatus). It also found that the P2 with a 50% concentration of corn powder gave the widest mycelium growth. Keywords: corn powder; mycelium; Pleurotus ostreatus

Download Full-text

Maize Residue as a Viable Substrate for Farm Scale Cultivation of Oyster Mushroom(Pleurotus ostreatus)

Advances in Agriculture ◽

10.1155/2015/213251 ◽

2015 ◽

Vol 2015 ◽

pp. 1-6 ◽

Cited By ~ 5

Author(s):

Abena O. Adjapong ◽

Kwame D. Ansah ◽

Faustina Angfaarabung ◽

Henry O. Sintim

Keyword(s):

Rice Bran ◽

Pleurotus Ostreatus ◽

Oyster Mushroom ◽

Biological Efficiency ◽

Growth Media ◽

Organic Substrates ◽

Mushroom Cultivation ◽

Fruiting Bodies ◽

Growing Media ◽

Maize Cob

In the search for alternatives to sawdust as growing media in commercial mushroom cultivation, three organic substrates obtainable as crop residue, maize husk, maize cob, and maize stalk, with each being supplemented with rice bran, were evaluated as growth media for the oyster mushroom,Pleurotus ostreatus(Kummer). For the tested alternatives to sawdust, the harvested weight of fruiting bodies that sprouted on a kilogram maize husk media per crop (32.99 g) was the highest. Sawdust media supported significantly (P<0.001) heavier fruiting bodies (42.18) than the maize residues. The peak mushroom harvests for the various substrates were obtained between the first and seventh fruiting body flushes. The biological efficiency of the substrates, which measured usable nutrients indicated that maize stalk supplemented with rice bran, was 39% compared to that of the sawdust media (60%). The maize husk media and the maize cob media had biological efficiencies of 32% and 9.5%, respectively. These results indicate that two of the tested growing media (maize stalk or husk) produced mushrooms with yield characteristics that were comparable to the well-used sawdust in the cultivation of oyster mushrooms. The environmental and economic parameters involved in the use and carting of sawdust make these on-farm crop residues a viable alternative for mushroom cultivation in especially nonforest zones of Ghana.

Download Full-text

Performance of oyster mushroom (Pleurotus ostreatus) grown on different fingermillet husk substrates

Journal of Agriculture and Natural Resources ◽

10.3126/janr.v4i1.33370 ◽

2021 ◽

Vol 4 (1) ◽

pp. 291-300

Author(s):

Priyanka Sanjel ◽

Ram Kumar Shrestha ◽

Jiban Shrestha

Keyword(s):

Rice Bran ◽

Wheat Bran ◽

Pleurotus Ostreatus ◽

Finger Millet ◽

Growth And Yield ◽

Oyster Mushroom ◽

Control Treatment ◽

Biological Efficiency ◽

Oilseed Cake ◽

Mushroom Yield

Substrate type is one of the major factors affecting the growth and yield of oyster mushroom (Pleurotus ostreatus). Five substrates; Finger millet husk, Fingermillet husk + Molasses, Fingermillet husk + Rice bran, Fingermillet husk + Wheat bran, Fingermillet husk +Mustard oilseed cake, were evaluated for growth and productivity of oyster mushroom. The experiment was conducted in a completely randomized design with each treatment replicated seven times. Additives were added on finger millet husk @5% of its dry weight. Standard cultivation practice was followed with steam sterilization and spawning was done in poly bags containing 2.5 kg of wet substrate. Data were collected on time taken for full spawn run, fresh mushroom yield, biological efficiency and cropping duration upto three flushes. Molasses took the least duration (16.86 days) for full spawn run which was found to be statistically at par with wheat bran(17.13 days) and rice bran(17.75 days) whereas control treatment took the longest duration (21.62 days). Rice bran produced the highest fresh mushroom yield (793.04g/bag) with highest biological efficiency (137.92%), which was statistically at par with control, molasses and wheat bran. Similarly, rice bran had least cropping duration (66.62 days) followed by control (67.88 days), molasses (69.14 days), wheat bran (70.12 days) and mustard oilseed cake (73.86 days). The lowest fresh yield (521.84 g/bag) with lowest biological efficiency (90.75%) and the longest cropping duration (73.86 days) was observed on mustard oilseed cake supplementation. This study revealed that molasses, rice bran and wheat bran accelerated spawn run whereas mustard oilseed cake supplementation produced lowest fresh mushroom yield with least biological efficiency and highest cropping duration showing that it was ineffective for increasing yield and productivity of oyster mushroom.

Download Full-text

Formulation of Inoculum a white oyster Mushroom (Pleurotus ostreatus) on powder

Biocelebes ◽

10.22487/bioceb.v13i3.14972 ◽

2019 ◽

Vol 13 (3) ◽

pp. 288-296

Author(s):

Khadijah ◽

Umrah ◽

Orryani Lambui

Keyword(s):

Production Process ◽

Incubation Period ◽

Rice Bran ◽

Incubation Time ◽

Natural Science ◽

Pleurotus Ostreatus ◽

Oyster Mushroom ◽

Viability Test ◽

Mushroom Production ◽

Randomized Design

The study about the formulation of inoculum a white oyster mushroom (Pleurotus ostreatus) on flour. was conducted at Biotechnology laboratory of Departement , Faculty of Mathematic and Natural Science, Tadulako University. This study were aimed to oyster mushroom on production. on and to abridge the cycle of oyster mushroom production process of white.peplication used Compeletely Randomized Design (CRD) consist of six treatments and tree by comparing between (sawduts,rice,bran, and comflour) P1(50% : 27% : 23% , P2 (50% : 30% : 20%), P3 (50% : 33% : 17%), P4 (50% : 36% : 14% ), P5 (50% : 39% : 11%), P6 (50% : 42% : 8%). There are four parameters used in this study (1) .The growth of white oyster mushroom inoculum (2) the incubation time. and, (3) coloni forming unit (CFU), (4) Viability test .The resulth showed that the best growth mycelium was 1,3cm/days with incubation period was 26 days, the highest total of CFU in P5 was 12,7x 108 CFU/ml and was in P3 was 3,3 x 108 CFU/ml .The inoculum viability test of production media in P5, was faster in incubation period is 18 days.

Download Full-text

Effect of Various Substrates on Growth and Yield Performance of Oyster Mushroom (Pleurotus ostreatus) in Chitwan, Nepal

International Journal of Applied Sciences and Biotechnology ◽

10.3126/ijasbt.v6i3.20859 ◽

2018 ◽

Vol 6 (3) ◽

pp. 215-219

Author(s):

Hom Prasad Sitaula ◽

Roshan Dhakal ◽

Geetesh DC ◽

Dharmendra Kalauni

Keyword(s):

Pleurotus Ostreatus ◽

Growth And Yield ◽

Oyster Mushroom ◽

Biological Efficiency ◽

Paddy Straw ◽

Yield Performance ◽

Randomized Design ◽

Stalk Diameter ◽

Maize Cob ◽

Colonization Period

An experiment was conducted at Rampur, Chitwan, Nepal to find out the growth and yield performance of oyster mushroom (Pleurotus ostreatus) in Completely Randomized Design (CRD).The treatment includes the four different substrate i.e. paddy straw (100%), maize cob+ paddy straw (1:1), sugarcane bagasses+paddy straw (1:1) and sawdust+ paddy straw (1:1). The parameters taken for the observation during the experiment were colonization period, fruit initiation period, length of stalk, diameter of stalk, diameter of pileus, fresh weight of the first and second flush of mushroom and also the biological efficiency (BE) of various substrates. Among the used substrates, the time for colonization and fruit initiation was found to be shorter in case of the paddy straw i.e.18.25 days and 21.75 days respectively. However, the length of stalk was highest in sugarcane bagasses+paddy straw (1:1) i.e.6.10 cm, but the diameter of stalk and pileus were highest in paddy straw i.e.0.80 cm and 7.90 cm respectively. Similarly, the biological efficiency was found to be highest in case of the paddy straw (96.29688%) followed by maize cob+paddy straw (1:1), sugarcane bagasses+ paddy straw (1:1) and sawdust+ paddy straw (1:1) respectively.Int. J. Appl. Sci. Biotechnol. Vol 6(3): 215-219

Download Full-text

Oyster Mushroom (Pleurotus ostreatus) Cultivation Using Sawdust and Different Organic Manures

Asian Food Science Journal ◽

10.9734/afsj/2021/v20i530299 ◽

2021 ◽

pp. 67-74

Author(s):

O. A. Orngu ◽

I. E. Mbaeyi-Nwaoha ◽

B. O. Unagwu ◽

V. E. Etim

Keyword(s):

Pleurotus Ostreatus ◽

Total Yield ◽

Good Alternative ◽

Oyster Mushroom ◽

Growth Time ◽

Average Weight ◽

Cow Dung ◽

Organic Manures ◽

Saw Dust ◽

Significant Difference

Aims: The aim was to evaluate the performance of cultivating oyster mushroom (Pleurotus ostreatus) using sawdust and different organic manures. Study Design: The experimental design used was the complete randomized design (CRD) and the Data obtained was subjected to Analysis of Variance (ANOVA) followed by Turkey’s Least Significant Difference(LSD) test to compare treatment means; differences was considered significant at 95% (P≤0.05) (SPSS Version 21 software). Place and Duration of Study: National Biotechnology Development Agency, South East Center, University of Nigeria, Nsukka between September 2019 and November 2019. Methodology: Saw dust was prepared as substrate with different organic manures and coded SD, Saw dust; SDP, Sawdust+ Poultry; SDC, Saw dust + cow dung; SDD, Saw dust+ pig dung) in the ratio of 100:0 and 50:50 respectively to cultivate oyster mushroom (Pleurotus ostreatus). Subsequently, the rate of growth, time of harvest, yield and average weight were recorded and proximate composition determined using standard methods. Results: Oyster mushroom harvested showed Moisture content ranged (4.63-7.14%), ash content (4.84-6.77%), crude fat (0.98-3.28%), fiber (16.02-18.23%), protein (19.27-33.41%) and carbohydrate (38.18%-48.89%). Average weight yield was highest in saw dust substrate (10.2g) with total yield (980g) and least average weight (7.5g) and total yield (105g) in sawdust and poultry litters substrate. Oyster mushroom from all the substrate differed significantly (p<0.05). Conclusion: Sawdust was the most suitable substrate for oyster mushroom cultivation though sawdust and pig dung combination can be a good alternative.

Download Full-text

Growth performance and blood profile of rabbit bucks in two housing types on aqueous extract of oyster mushroom (Pleurotus ostreatus Jacq ex fr.)

Nigerian Journal of Animal Production ◽

10.51791/njap.v46i1.1316 ◽

2020 ◽

Vol 46 (1) ◽

pp. 51-60

Author(s):

O. M. Sogunle ◽

T. S. Aremu ◽

O. O. Olatubosun ◽

C. P. Njoku ◽

O. M. Sogunle ◽

...

Keyword(s):

Growth Performance ◽

Pleurotus Ostreatus ◽

Octadecadienoic Acid ◽

Conversion Ratio ◽

Oyster Mushroom ◽

Feed Conversion ◽

Blood Profile ◽

Mushroom Extract ◽

Randomized Design ◽

Completely Randomized Design

The growth performance and blood profile of forty-eight (48), six (6) weeks old rabbit bucks of cross (Chinchilla and New Zealand White) were assessed for 10 weeks. The bucks were allotted on weight equalization basis in a 2 x 4 factorial experimental layout composed of 24 rabbits into steel hutches and wooden hutches each and on four (4) varying levels (0, 5, 10 and 15 mL/litre of water, respectively) of oyster mushroom (Pleurotus ostreatus Jacq ex fr.) extract. Six (6) bucks were assigned to each treatment group and replicated three times with two (2) bucks per replicate in a Completely Randomized Design. The phytochemicals of the oyster mushroom extract showed that 9,12-Octadecadienoic acid (Z, Z)- methyl ester was the most abundant. Results showed that the feed conversion ratio was significantly (P<0.05) best (6.08) in bucks reared in wooden cage and administered 15 mL oyster mushroom extract. Significantly (P<0.05) highest triglycerides (81.00 mg/dL) was obtained in bucks on steel hutch and on 5 mL oyster mushroom extract and lowest (55.00 mg/dL) in bucks on steel hutch and on 10 mL oyster mushroom extract. The lowest (P<0.05) lymphocytes (59.00%) were recorded in bucks on wooden cage and administered 10 mL oyster mushroom extract. It was concluded that oyster mushroom extract up to 15 mL/litre of water administered in either steel hutch or wooden hutch did not impair the growth performance and blood profile of rabbit bucks.

Download Full-text

DEKOLORISASI LIMBAH BATIK MENGGUNAKAN LIMBAH MEDIUM TANAM Pleurotus ostreatus PADA WAKTU INKUBASI YANG BERBEDA

Scripta Biologica ◽

10.20884/1.sb.2014.1.1.29 ◽

2014 ◽

Vol 1 (1) ◽

pp. 73

Author(s):

Fitriya Yuni Wulandari ◽

Nuniek Ina Ratnaningtyas ◽

Ratna Stia Dewi

Keyword(s):

Incubation Time ◽

Confidence Level ◽

Pleurotus Ostreatus ◽

Waste Treatment ◽

Time Treatment ◽

Randomized Design ◽

Significant Difference ◽

Treatment Technologies ◽

Difference Test ◽

Honest Significant Difference Test

The batik waste industry is one source of water pollution. It must undergo treatment before discharge into the environment. Batik waste treatment technologies can be done biologically, chemically, physically, or combination of three processes. This research applied a combination of biology and physic technologies, namely used spent mushroom of Pleurotus ostreatus as the decolorization agent. Mycelium of P. ostreatus was used as biological agent, and cellulose from spent mushroom was used as physical agent. The aims of this research were to determine the effect of spent mushroom with variation incubation time on batik waste decolorization, and to know the optimum incubation time with highest decolorization batik waste percentage using spent mushroom of P. ostreatus. This research used experimental method with completely randomized design (CRD). The data obtained were statistically analyzed using Varian Test (ANOVA) on confidence level of 95% and 99% and continued with Honest Significant Difference Test (BNJ) with 95% confidence level. The result showed that variation incubation time treatment of batik waste decolorization used spent mushroom of P. ostreatus provide a highly signification effect on batik waste decolorization. The optimum incubation time was 60 hour (LW5), with decolorization percentage was 85,64%.

Download Full-text

Grey Oyster Mushroom Biofarm for Small-Scale Entrepreneurship

Advances in Agriculture ◽

10.1155/2019/6853627 ◽

2019 ◽

Vol 2019 ◽

pp. 1-6

Author(s):

Desta Berhe Sbhatu ◽

Haftom Baraki Abraha ◽

Hiluf Tekle Fisseha

Keyword(s):

Wheat Bran ◽

Oyster Mushroom ◽

Small Scale ◽

Optimum Conditions ◽

Home Based ◽

Randomized Design ◽

Polyethylene Bags ◽

Completely Randomized Design ◽

Aseptic Conditions ◽

Growing Mass

This paper reports the productivity of a small-scale pilot biofarm of grey oyster mushroom (Pleurotus sajor-caju (Fr.) Sing.). The pilot was tested in Mekelle city (Ethiopia) in a brick-walled dark room. Growing structures were constructed by erecting three wood poles and fixing them with three wooden side bars at multiple locations to make a prism-shaped rack with multiple triangular open shelves, each capable of carrying one bag of spawned substrate. Mushroom substrates were prepared from maize stalk and wheat bran supplement. Pasteurized chopped maize stalk and wheat bran were mixed at the ratio of 10:0, 9:1, 8:2, and 7:3—yielding four treatments. Five kilograms of substrate was taken from each treatment and was mixed with one kilogram of gypsum to produce a growing mass. Each mass was spawned with 200 g of inoculum under aseptic conditions and put in polyethylene bags. The treatments were replicated thrice and the bags were put on the growth racks in completely randomized design. The growing room was maintained at optimum conditions. Maize stalk substrates supplemented with 10% and 20% of wheat bran have resulted in statistically comparable productivities but statistically significantly higher than those grown on nonsupplemented and highly supplemented maize stalk substrates (p ≤ 0.05). The ingenuity of the design and the convenience of the construction of the racks, the availability of the substrates, and the simplicity of the management and maintenance of the biofarm rendered the piloted design suitable for home-based and small- and medium-scale mushroom biofarm entrepreneurship.

Download Full-text

MORPHOLOGICAL RESPONSES OF KING OYSTER MUSHROOM AGAINST AUGMENTATION OF COTTON WASTE WITH CITRUS PEEL

Pakistan Journal of Phytopathology ◽

10.33866/phytopathol.030.01.0442 ◽

2018 ◽

Vol 30 (1) ◽

pp. 99

Author(s):

Muhammad M. Jahangir ◽

Muhammad A. Khatana ◽

Nasir A. Khan ◽

Ghufrana Samin ◽

Khurram Ziaf ◽

...

Keyword(s):

Oyster Mushroom ◽

Biological Efficiency ◽

Mycelium Growth ◽

Citrus Peel ◽

Morphological Attributes ◽

Mean Values ◽

Randomized Design ◽

Cotton Waste ◽

Growth Initiation ◽

King Oyster Mushroom

The utilization of agro-industrial waste materials for production of mushrooms has become a crucial research precedence recently. Pleurotus eryngii is considered one of the most famous mushrooms because of nutraceutical and high nutritional value. Therefore, a study was conducted in Medicinal and Mushroom Lab to evaluate the morphological attributes of king oyster mushroom against cotton waste (CW) and citrus peel (CP) based substrates. CW alone and its different combinations with CP i.e. T0: CW (100 %), T1: CW (98%) + CP (2%), T2: CW (96%) + CP (4%), T3: CW (94%) + CP (6%), T4: CW (92%) + CP (8%) and T5: CW (90%) + CP (10%) were used in experiment. The experiment was conducted by applying 2-factor factorial under completely randomized design with 5 replications. Different morphological attributes viz. spawn running and mycelium growth initiation, duration to complete mycelium growth (days), pinhead development, pinheads/bag, duration to complete flushes (1st,2nd, 3rd), mushroom fresh weight, yield/bag and biological efficiency were calculated. Results were analyzed by ANOVA and mean values are equated by LSD test at 5% probability level. Results indicated that spawn running, the growth of mycelium, duration to complete flushes, pinhead development and duration, yield and biological efficiency all were significantly influenced by treatment T2 (CW 96% + CP 4%) as compared to control. Overall results revealed that combinations of citrus peel and cotton waste can be utilized as a promising substrate for king oyster mushroom production.

Download Full-text