Occurrence of Enteric Bacteria and Viruses in Oysters

1980 ◽  
Vol 43 (2) ◽  
pp. 111-113 ◽  
Author(s):  
MARK D. SOBSEY ◽  
CAMERON R. HACKNEY ◽  
ROBERT J. CARRICK ◽  
BIBEK RAY ◽  
MARVIN L. SPECK
Keyword(s):  
Fecal Coliform ◽  
Enteric Bacteria ◽  
Total Coliform ◽  
Fecal Pollution ◽  
Enteric Pathogens ◽  
The Other ◽  
Fecal Coliforms ◽  
Sample Collection ◽  
Closed Area ◽  
Vibrio Parahemolyticus

Enteric bacteria and virus levels were determined in oysters from paired stations that were opened or closed for commercial shellfishing on the basis of total coliform levels in the water. Six pairs of stations were sampled quarterly over a 1-year period. Enteric viruses were found in 3 of 24 50-g oyster samples from closed areas and in none of 23 samples from open areas. Salmonella was found in 2 of 47 samples of 40 g each, one from an open and the other from a closed area. Although enteric pathogens of fecal origin were found only in oysters that exceeded the recommended market limit of 230 fecal coliforms per 100 g of meat, the fecal coliform levels in some virus-positive samples were much lower than those in Salmonella-positive samples. Vibrio parahemolyticus levels were similar in oysters from both open and closed beds, indicating no particular association with fecal pollution. However, there was a marked seasonal variation in V. parahemolyticus levels. Total but not fecal coliform levels in oysters from open beds correlated with the occurrence of rainfall 1 or 2 days before sample collection. Neither total nor fecal coliform levels in oysters from closed beds correlated with rainfall. These findings suggest that fecal coliforms levels in oysters are less influenced by rainfall than are total coliforms, and therefore may be a more specific indicator of recent fecal pollution.

Microbiological performance and salmonella dynamics in a wastewater depuration pond system of southeastern spain

1995 ◽  
Vol 31 (12) ◽  
pp. 239-248 ◽  
Author(s):  
Ana Emparanza-Knörr ◽  
Francisco Torrella
Keyword(s):  
Fecal Coliform ◽  
No Reduction ◽  
Agar Medium ◽  
Microbiological Quality ◽  
Total Coliform ◽  
Fecal Coliforms ◽  
Selective Media ◽  
E Coli ◽  
The Village ◽  
Final Effluent

The Salmonella presence and the microbiological quality indicators, total and fecal coliforms and coliphages of E. coli C, have been studied in a overloaded wastewater lagoon system treating urban wastewatrers of the village of Guardamar del Segura (Alicante, Spain). Classical microbiological technology to detect salmonellae was used, including pre-enrichment, enrichment, selective media plating and biochemical and serological confirmation. Water was physicochemically characterized according to COD, SS, temperature, pH and dissolved oxygen. The selective migration step through Rappaport-Vassiliadis semisolid agar medium was essential for the consistent detection of Salmonella in the different lagoon effluents. Total and fecal coliform levels of up to 105-106 MPN/100 ml were detected in the final effluent. High coliphage concentrations of 103-104 pfu/ml were also found in the effluent waters. Salmonella was always detected in 100 ml samples and eventually reached an order of value of 103 MPN/100 ml. Total coliform reduction was higher in the anaerobic ponds whereas fecal coliforms were more efficiently eliminated in the facultative (mostly “anoxic”) lagoons. Coliphage reduction was higher in the facultative lagoons when compared to the anaerobic ponds. On many occasions, no reduction or eventual increment of the concentration of salmonellae was detected in the effluents from the anaerobic ponds compared to concentrations of the patohogen in the influent raw wasterwaters. The possibility exists for a capacity of Salmonella to multiply in the anoxic phase of the wastewater treatment, but the presence of microorganisms in raw sewage waters which could maskSalmonella detection with the enrichment methodology employed cannot be ruled out.

Validity of Members of the Total Coliform and Fecal Coliform Groups for Indicating the Presence of Salmonella in the Quahaug, Mercenaria mercenaria

1976 ◽  
Vol 39 (5) ◽  
pp. 322-324 ◽  
Author(s):  
W. H. ANDREWS ◽  
C. D. DIGGS ◽  
J. J. MIESCIER ◽  
C. R. WILSON ◽  
W. N. ADAMS ◽  
...  
Keyword(s):  
Fecal Coliform ◽  
Mercenaria Mercenaria ◽  
Quality Standard ◽  
Total Coliform ◽  
Fecal Coliforms ◽  
Most Probable Number ◽  
Wholesale Market ◽  
Probable Number ◽  
Hard Shell ◽  
Relationship Of

To determine the relationship of most probable number (MPN) of the total and fecal coliform groups in shellfish and shellfish-growing waters to the presence of Salmonella in quahaugs (Mercenaria mercenaria), a microbiological survey of 214 samples of the quahaug, or hard-shell clam, was done over 24 months. For purposes of this study, waters were classified as safe for shellfish harvesting by one of two criteria: (a) a total coliform MPN of ≤ 70/100 ml of water or (b) a fecal coliform MPN value of ⩽ 14/100 ml of water. None of the quahaug samples harvested from waters meeting these standards contained Salmonella. Additionally, Salmonella was not detected in any of the quahaug samples meeting the wholesale market quality standard of 230 fecal coliforms per 100 g of shellfish as specified by the National Shellfish Sanitation Program. An increase in the total coliform and fecal coliform MPN of the waters more closely paralleled an increase in the fecal coliform MPN, as compared to the total coliform MPN of the quahaug meats. Five Salmonella serotypes, including Salmonella parathyphi B, were found singly and in combination in five (2.3%) of the quahaug samples in this survey.

scholarly journals Comparison of Membrane Filtration and Replica Plate Technique to Detect Fecal Coliform

2018 ◽  
Vol 17 (2) ◽  
pp. 25-31 ◽  
Author(s):  
Bikram Gautam ◽  
Rameshwar Adhikari
Keyword(s):  
Fecal Coliform ◽  
Membrane Filtration ◽  
Pearson Correlation ◽  
Total Coliform ◽  
Fecal Coliforms ◽  
Kathmandu Valley ◽  
Local Market ◽  
Extrinsic Factors ◽  
Colony Forming Units ◽  
Routine Basis

Introduction: Membrane filtration (MF) is one of the widely used technique on a routine basis. On the other hand, replica plate (RP) technique can be used to transfer existing bacterial colonies in two plates which even allows pinpointing the original colony. The aim of this study is to comparatively detect the cfu/100 mL of fecal coliform using membrane filtration and replica plate techniques.Methods: In the study, a total of 25 bottled water were selected from the local market in Kathmandu valley. The total coliform count was detected using MF, while fecal coliform was detected using both MF and RP technique.Results: It was found that the average cfu/100 mL for total coliform, fecal coliform (MF) and fecal coliform (RP) were 143.38, 49.82 and 51.00 respectively. Pearson correlation coefficient calculated between total coliform and fecal coliform (MF), total coliform and fecal coliform (RP), fecal coliform (MF) and fecal coliform (RP) were found to be 0.695, 0.733 and 0.990 respectively; implying a positive correlation Conclusions: It has been demonstrated that intrinsic and extrinsic factors influence colony forming units. Furthermore, RP is a more sensitive method for screening fecal coliforms although both MF and RP can be efficiently used.

scholarly journals Toxicological risks of water and human health, contribution to the biological treatments in the dam of Ain Zada

2017 ◽  
Vol 1 (2) ◽  
pp. 35-36
Author(s):  
Bariza Gourari ◽  
Djamel Anteur
Keyword(s):  
Fecal Coliform ◽  
Pathogenic Bacteria ◽  
Culture Medium ◽  
Health Hazard ◽  
Clarias Gariepinus ◽  
Total Coliform ◽  
Fecal Coliforms ◽  
Ctenopharyngodon Idella ◽  
Hypophthalmichthys Molitrix ◽  
Fecal Streptococci

Background: Freshwater fish play a role in detoxification of certain germs and pathogens of bacteria. Variation of the content of pathogenic bacteria are, mainly, total coliform, fecal coliform, and fecal streptococci in the study sites of the sampling period. The objective of this study is inventoried purifying species of dam of Ain Zada. Methods: The isolation of fecal coliform (CF) and total coliform (CT) was conducted by incorporation in agar of the five shot test (0,4 mL; 1 mL et 3 x 2 mL). The culture medium is the selective agar Mac Conkey (HIMEDIA, M083) coliforms. The enumeration of the colonies is done after 24 of incubation at 37°C for total coliforms and 44°C for fecal coliforms. The isolation of fecal streptococci (SF) is made by the same method but on the culture medium incubated at 37°C for 48 hours. Counting of colonies is done on mailboxes where 100 to 300 colonies are developed. The average of colonies counted is then determined. The boxes do not contain at least 100 colonies, all of the colonies on all boxes are counted, taking into account the total volume inoculated in water. In the second part, there is an inventory of purifying fish at the Ain Zada dam level. Results: The result indicate that all the fish that play a role in the treatment of dam of Ain Zada (Bordj Bou Arreridj) who use these germs and bacteria as a food source. We registered the presence of a single family, with five (05) genera divided into seven (07) species, including the majority of alien species are Cyprinid fish: Sander lucioperca L., Ctenopharyngodon idella, Clarias gariepinus, Cyprinus carpio, Hypophthalmichthys molitrix and Hypophtalmichthys nobilis. Conclusion: The Bousselam and Ouricia valleys feature heavily poor microbiological quality; exploitation of these waters could constitute a health hazard on dam Ain Zada. According to this study, the fish play a role of biological control.

Yeasts and coliform bacteria of water accumulated in bromeliads of mangrove and sand dune ecosystems of southeast Brazil

1993 ◽  
Vol 39 (10) ◽  
pp. 973-977 ◽  
Author(s):  
Allen N. Hagler ◽  
Carlos A. Rosa ◽  
Paula B. Morais ◽  
Leda C. Mendonça-Hagler ◽  
Georgia M. O. Franco ◽  
...  
Keyword(s):  
Fecal Coliform ◽  
Sand Dune ◽  
Mangrove Ecosystem ◽  
Total Coliform ◽  
Fecal Coliforms ◽  
Coliform Bacteria ◽  
E Coli ◽  
Yeast Community ◽  
The Mean ◽  
Ascomycetous Yeasts

Yeasts and coliform bacteria were isolated from water that accumulated in the central cups and adjacent leaf axilae of two bromeliads, Neoregelia cruenta of a coastal sand dune and Quesnelia quesneliana of a mangrove ecosystem near the city of Rio de Janeiro, Brazil. The mean total coliform counts were above 10 000 per 100 mL for waters of both plants, but the mean fecal coliform counts were only 74 per 100 mL for Q. quesneliana and mostly undetected in water from N. cruenta. Of 90 fecal coliform isolates, 51 were typical of Escherichia coli in colony morphology and indol, methyl red, Volges–Proskauer, and citrate (IMViC) tests. Seven representatives of the typical E. coli cultures were identified as this species, but the identifications of nine other coliform bacteria were mostly dubious. The yeast community of N. cruenta was typical of plant surfaces with basidiomycetous yeasts anamorphs, and the black yeast Aureobasidium pullulans was prevalent. Quesnelia quesneliana had a substantial proportion of ascomycetous yeasts and their anamorphs, including a probable new biotype of Saccharomyces unisporus. Our results suggested that the microbial communities in bromeliad waters are typically autochtonous and not contaminants.Key words: yeasts, fecal coliforms, bromeliad waters, mangrove, Restinga.

Surface Contamination of Beef Carcasses by Fecal Coliforms

1991 ◽  
Vol 54 (12) ◽  
pp. 950-956 ◽  
Author(s):  
R. CHARLEBOIS ◽  
R. TRUDEL ◽  
S. MESSIER
Keyword(s):  
Relative Humidity ◽  
Fecal Coliform ◽  
The Other ◽  
Fecal Coliforms ◽  
Meat Processing ◽  
Coliform Count ◽  
Beef Carcasses ◽  
Significant Difference ◽  
The Times ◽  
Processing Steps

A total of 18 half-carcasses was selected at random in each of three different meat processing establishments. Samples were taken by excision at seven sites on each half-carcass at the end of four processing sectors, from the dressing of the carcass to the packaging of boneless beef, and the fecal coliform counts per cm2 were subsequently determined. A statistically significant difference in mean fecal coliform count was found between the reference and the other two establishments. Among the four processing steps, boning operations contributed the most to the final count of fecal coliforms on boneless beef. Generally speaking, among the sites, the flank recorded the highest mean fecal coliform count per cm2; nonetheless, following boning operations, the semitendinosus area demonstrated a mean count significantly higher than all the other sites, with the sole exception of the rump. No consistent differences in mean fecal coliform counts were found between the days or the times of sampling, nor between the front and hind quarters. No meaningful linear correlation was detected between the fecal coliform counts and the factors under study, i.e., length of time a carcass is kept in the evisceration area, relative humidity of the evisceration area, warm carcass weight, duration of cold storage, and temperature and relative humidity of the boning room. In summary, the results indicate that any attempt at successfully minimizing boneless beef contamination by fecal coliforms requires the application of tighter control over cross-contamination at the boning room level.

Variation in fecal pollution indicators through tidal cycles in the Fraser River estuary

1982 ◽  
Vol 28 (2) ◽  
pp. 239-247 ◽  
Author(s):  
Leslie M. Churchland ◽  
Gordon Kan ◽  
Alard Ages
Keyword(s):  
Fecal Coliform ◽  
Sewage Treatment ◽  
River Estuary ◽  
Fecal Pollution ◽  
Water Levels ◽  
Fecal Coliforms ◽  
Fraser River ◽  
Fecal Sterols ◽  
Pollution Indicators ◽  
Highly Correlated

Daily fluctuations in fecal pollution indicators in the Fraser River estuary were examined by sampling through 24 h at four sites; water levels, currents, and salinities were observed simultaneously. Fecal coliforms were measured on shipboard using the membrane filter technique, and coprostanol and cholesterol were extracted using hexane and analyzed by gas chromatography. Maximum concentrations of fecal sterols and coliforms occurred after high tide at the station upstream of major sewage outfalls and on the ebb or at low tide at the stations downstream of major sewage outfalls. Fecal coliform counts were highly correlated with coprostanol and cholesterol levels at sites near sewage treatment plants. Coprostanol was highly correlated with cholesterol except where concentrations approached detection limits. In receiving waters, fecal coliform counts but not sterol concentrations were reduced by chlorination of sewage treatment plant effluents during the summer months. We concluded that, dependent on the objectives of a future monitoring program, samples should be collected either randomly in time such that the seasonal or annual mean includes tidal variability or systematically during the ebb tide to assess contributions to bathing beaches and shellfish harvesting areas. Fecal sterols deserve consideration as potential indicators of fecal contamination from chlorinated sewage effluents.

Enteric Bacterial and Viral Pathogens and Indicator Bacteria in Hard Shell Clams

1983 ◽  
Vol 46 (6) ◽  
pp. 493-496 ◽  
Author(s):  
DOUGLAS A. WAIT ◽  
CAMERON RAY HACKNEY ◽  
ROBERT J. CARRICK ◽  
G. LOVELACE ◽  
MARK D. SOBSEY
Keyword(s):  
Enteric Viruses ◽  
Mercenaria Mercenaria ◽  
Enteric Bacteria ◽  
Total Coliform ◽  
Fecal Coliforms ◽  
Viral Pathogens ◽  
Total Coliforms ◽  
Significant Difference ◽  
Hard Shell ◽  
Plate Counts

Enteric bacteria and virus levels were determined in hard shell clams, Mercenaria mercenaria, harvested from areas open or closed for commercial shellfishing on the basis of total coliform levels in water. Four pairs of open and closed stations were sampled seasonally over a 1-year period. Enteric viruses were isolated from 3 of 13 100-g clam samples from open beds and 6 of 15 samples from closed beds. Salmonella was found in 1 of 15 samples from closed areas, but not in any samples from open areas. No Shigella or Yersinia were isolated from clams taken from either open or closed beds. Levels of Vibrio parahaemolyticus, an indigenous estuarine microorganism, were similar in clams from open and closed areas. No statistically significant difference was found in the occurrence of enteric viruses in clams from open and closed areas. Product-moment correlations between concentrations of enteric viruses and bacteria in clams or water demonstrated no statistically significant correlations between virus concentrations in clams and total coliforms or fecal coliforms in water or total coliforms, fecal coliforms, fecal streptococci or aerobic plate counts in clams.

Identification of strains isolated as total and fecal coliforms and comparison of both groups as indicators of fecal pollution in tropical climates

1983 ◽  
Vol 29 (6) ◽  
pp. 689-693 ◽  
Author(s):  
Marc C. Lavoie
Keyword(s):  
Fecal Coliform ◽  
Fecal Contamination ◽  
Total Coliform ◽  
Fecal Coliforms ◽  
Well Water ◽  
Human Feces ◽  
E Coli ◽  
Membrane Filters ◽  
Metallic Sheen ◽  
Tropical Climates

This study was undertaken to better characterize the groups of total coliforms (TC) and fecal coliforms (FC) and to evaluate both groups as indicators of fecal contamination of drinking well water in a tropical climate (The Ivory Coast, West Africa). Isolated colonies obtained as TC or FC on membrane filters were identified using the API-20E system. From the well water samples, 58 golden-green colonies with a metallic sheen isolated on Endo medium (TC) were identified as Escherichia coli (55%), Enterobacter (26%), Klebsiella (14%), Proteus (3%), and Citrobacter (2%). Among 132 colonies isolated on Endo medium as non-TC (not showing the characteristic golden metallic sheen), 10% were identified as E. coli. The 196 blue colonies isolated on M-FC medium at 44.5 °C (FC) were identified as E. coli (66%), Klebsiella (12%), Enterobacter (10%), Citrobacter (5%), Salmonella (3%), Serratia (3%), Proteus (2%), and Yersinia (0.5%). Among 24 nonblue colonies on M-FC medium, none were identified as E. coli. Of the colonies isolated from human feces, E. coli represents 92% of the TC and 89% of the FC. Although these results are limited, they tend to confirm the greater specificity of the fecal coliform technique over that of total coliform for the detection of fecal contamination of untreated well water. From the results presented here and the observations of other workers, it is suggested that the use of FC instead of TC should be considered as the method of choice for determining drinking water pollution of untreated groundwater supplies.

Single-stage Sequencing Batch Process for Producing Class A Biosolids from AD Effluent at Terminal Island Treatment Plant

2006 ◽  
Vol 1 (4) ◽  
Author(s):  
Huub H.J. Cox ◽  
Steve Fan ◽  
Reza Iranpour
Keyword(s):  
Fecal Coliform ◽  
Treatment Plant ◽  
Batch Process ◽  
Single Stage ◽  
Fecal Coliforms ◽  
Class A ◽  
Truck Loading ◽  
Train Operation ◽  
Plant Control ◽  
Class A Biosolids

Terminal Island Treatment Plant converted its digesters to thermophilic operation with the objective to comply with the U.S. EPA Part 503 Biosolids Rule requirements for Class A biosolids. The following processes were tested: a) single-stage continuous; b) two-stage continuous; c) single-stage sequencing batch. Salmonella sp. were always non-detect in digester outflows (<3 MPN/4 g dry wt), whereas fecal coliform densities were usually below the Class A limit of 1000 MPN/g dry wt. However, the recurrence of fecal coliforms in post-digestion caused non-compliance with the Class A limit at the truck loading facility as the last point of plant control for compliance. After several design modifications of the post-digestion train, operation of the digesters as sequencing batch digesters according to the time-temperature requirement of Alternative 1 of the Part 503 Biosolids Rule achieved compliance for both Salmonella sp. and fecal coliforms at the last point of plant control (truck loading facility).

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