Control of Aspergillus flavus in Maize with Plant Essential Oils and Their Components

1998 ◽  
Vol 61 (5) ◽  
pp. 616-619 ◽  
Author(s):  
R. MONTES-BELMONT ◽  
M. CARVAJAL
Keyword(s):  
Essential Oils ◽  
Aspergillus Flavus ◽  
Residual Effect ◽  
Residual Effects ◽  
Thymus Vulgaris ◽  
Optimal Dosage ◽  
Syzygium Aromaticum ◽  
Plant Essential Oils ◽  
Phytotoxic Effect ◽  
Maize Kernels

The effects of 11 plant essential oils for maize kemel protection against Aspergillus flavus were studied. Tests were conducted to determine optimal levels of dosages for maize protection, effects of combinations of essential oils, and residual effects and toxicity of essential oils to maize plants. Principal constituents of eight essential oils were tested for ability to protect maize kernels. Essential oils of Cinnamomum zeylanicum (cinnamon), Menthapiperita (peppermint), Ocimum basilicum (basil), Origanum vulgare (origanum), Teloxys ambrosioides (the flavoring herb epazote), Syzygium aromaticum (clove), and Thymus vulgaris (thyme) caused a total inhibition of fungal development on maize kernels. Thymol and o-methoxycinnamaldehyde significantly reduced maize grain contamination. The optimal dosage for protection of maize varied from 3 to 8%. Combinations of C. zeylanicum with the remaining oils gave efficient control. A residual effect of C. zeylanicum was detected after 4 weeks of kernel treatment. No phytotoxic effect on germination and com growth was detected with any of these oils.

scholarly journals Effects of selected plant essential oils on the growth and development of mouse preimplantation embryos in vivo

2007 ◽  
pp. 97-104
Author(s):  
M Domaracký ◽  
P Rehák ◽  
Š Juhás ◽  
J Koppel
Keyword(s):  
Cell Death ◽  
Essential Oils ◽  
Embryo Development ◽  
Growth And Development ◽  
Fluorescent Microscopy ◽  
Preimplantation Embryos ◽  
Thymus Vulgaris ◽  
Syzygium Aromaticum ◽  
Plant Essential Oils

Plant essential oils (EOs) have been reported to have health benefit properties and their preventive and therapeutic use in animals is expected to increase in the future. We evaluated the influence of five essential oils obtained from plant species which are known to have positive antimicrobial, antioxidative and anti-inflammatory effects--sage EO from Salvia officinalis L. (Lamiaceae), oregano EO from Origanum vulgare L. (Lamiaceae), thyme EO from Thymus vulgaris L. (Lamiaceae), clove EO from Syzygium aromaticum L. (Myrtaceae) and cinnamon EO from Cinnamomum zeylanicum Blume (Lauraceae) on the growth and development of mouse preimplantation embryos in vivo. Essential oils were added to commercial diet at concentrations of 0.25% for sage EO, thyme EO, clove EO, cinnamon EO and 0.1% for oregano EO, and fed to ICR female mice for 2 weeks ad libitum. Females were then mated with males of the same strain. Embryos obtained on Day 4 of pregnancy at the blastocyst stage were stained by morphological triple staining (Hoechst, PI, Calcein-AM) and evaluated using fluorescent microscopy. The effects of essential oils were estimated by the viability of embryos, number of nuclei and distribution of embryos according to nucleus number. Cinnamon EO significantly decreased the number of nuclei and the distribution of embryos according to nucleus number was significantly altered. Sage EO negatively influenced the distribution of embryos according to nucleus number. Clove and oregano EOs induced a significantly increased rate of cell death. Only thyme EO had no detectable effects on embryo development. In conclusion, none of the essential oils had any positive effect on embryo development, but some of them reduced the number of cells and increased the incidence of cell death.

scholarly journals Sensitivity to Fungicides and Esential Oils in Czech Isolates of Phytophthora infestans

2018 ◽  
Vol 49 (2) ◽  
pp. 69-77
Author(s):  
J. Mazáková ◽  
M. Zouhar ◽  
P. Sedlák ◽  
E. Zusková ◽  
P. Ryšánek ◽  
...  
Keyword(s):  
Essential Oils ◽  
Phytophthora Infestans ◽  
Mycelial Growth ◽  
Thymus Vulgaris ◽  
Syzygium Aromaticum ◽  
Mentha Spicata ◽  
Minimal Inhibitory Concentrations ◽  
Plant Essential Oils ◽  
Agar Method

Abstract A total of 235 Phytophthora infestans isolates were collected from five regions of the Czech Republic during the growing seasons 2012–2014 and 2016 and examined using the in vitro amended agar method for their sensitivity to metalaxyl-M (MFX), propamocarb-HCl (PCH), and dimethomorph (DMM). A majority of the isolates (50%) were sensitive to MFX. Resistant isolates were found in all four years of the survey; they represented 30% of the samples. The EC50 values of PCH in inhibiting mycelial growth of 65% of the overall isolates were higher than 100 μg ml−1, which indicates the occurrence of insensitivity to PCH in the Czech P. infestans populations. DMM was very effective, and the mycelial growth of all isolates tested was completely suppressed at the concentration of 0.1 μg ml−1. Furthermore, the efficacy of 12 plant essential oils was tested against 20 isolates of P. infestans using the in vitro amended agar method. Essential oils of Cymbopogon winterianus, Litsea cubeba, Mentha spicata, Pelargonium graveolens, Syzygium aromaticum, and Thymus vulgaris were observed to have the highest antifungal activity against P. infestans, with minimal inhibitory concentrations less than or equal to 1 μl ml−1.

scholarly journals The Effect of Essential Oil Intake on Changes of Plasma Antioxidant Status in Mice

2007 ◽  
Vol 76 (3) ◽  
pp. 357-361 ◽  
Author(s):  
Š. Faix ◽  
Š. Juhas ◽  
Z. Faixová
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
The Body ◽  
Thymus Vulgaris ◽  
High Dose ◽  
Syzygium Aromaticum ◽  
Lower Growth ◽  
Total Antioxidant

The aim of this study was to determine the effects of four essential oils intake by feed, namely Origanum vulgare, Thymus vulgaris, Cinnamomum zeylanicum Ness, and Syzygium aromaticum on antioxidant status in mice in vivo. Essential oils were in the aether oleum form. They were diluted with ethanol absolute mixed with ground pelett (0.1, 0.25, 0.57 and 1% concentration) and thereafter ethanol was evaporated. SOD, GPx activities and TAS were measured in erythrocytes and plasma spectrophotometrically with Ransod, Ransel and TAS kits from RANDOX, respectively. GPX activity showed a significant increase in 0.25% and 0.1% concentration of Origani aetheroleum. The GPx activities were decreased in 1% concentration of Thymi aetheroleum and 0.57% concentration of Cinnamomi aetheroleum and 0.57% concentration of Caryophylli aetheroleum. The total antioxidant status showed a significant decrease in 1 % concentration of Origani aetheroleum and significantly increased in 0.1% concentration. The same results were found in Thymi aetheroleum. Cinnamomi aetheroleum and Caryophylli aetheroleum had not effect on total antioxidant status. SOD activities were not significantly changed after intake of essential oils. In conclusion, our results showed, that concentration of essential oil is very important for antioxidant status and also for metabolism of mice, because a high dose of essential oil has adverse effect on metabolism of mice, representated by a lower growth of the body weight. On the other hand, essential oils at lower concentrations have positive effect on antioxidant status of mice.

scholarly journals Effect of plant essential oils on mortality of the stem nematode (Ditylenchus dipsaci)

2009 ◽  
Vol 45 (No. 2) ◽  
pp. 66-73 ◽  
Author(s):  
M. Zouhar ◽  
O. Douda ◽  
D. Lhotský ◽  
R. Pavela
Keyword(s):  
Essential Oils ◽  
Nematicidal Activity ◽  
Thymus Vulgaris ◽  
Aromatic Plants ◽  
Origanum Vulgare ◽  
Medicinal And Aromatic Plants ◽  
Plant Essential Oils ◽  
Nematode Management ◽  
Origanum Compactum ◽  
Ditylenchus Dipsaci

The aim of this study was to assess the nematicidal activity of different essential oils from medicinal and aromatic plants for use in nematode management. Essential oils of <I>Eugenia caryophyllata, Origanum compactum, Origanum vulgare, Thymus matschiana</I> and <I>Thymus vulgaris</I> showed nematicidal activity against <I>Ditylenchus dipsaci.</I>

scholarly journals Seeds of Calotropis procera Treated With Essential Oils of Copaifera langsdorffii Desf. and Syzygium aromaticum L.

2018 ◽  
Vol 11 (1) ◽  
pp. 438
Author(s):  
Ronimeire T. da Silva ◽  
Maria de F. Q. Lopes ◽  
Miguel A. Barbosa Neto ◽  
Otília R. de Fárias ◽  
Luciana C. do Nascimento ◽  
...  
Keyword(s):  
Essential Oils ◽  
Dry Mass ◽  
Calotropis Procera ◽  
Soil Conditions ◽  
Syzygium Aromaticum ◽  
Phytotoxic Effect ◽  
Copaifera Langsdorffii ◽  
Physiological Quality ◽  
Sexual Propagation ◽  
Semi Arid Region

The Silk Flower (Calotropis procera) is widely used by farmers in the Northeast region, due to the adaptation of the climatic and soil conditions of the semi-arid region, but the incidence of pathogens has reduced the physiological and sanitary quality standards acceptable for sexual propagation of these plants in the field. Thus, the objective of this research was to verify the effects of the Copa&iacute;ba (C. langsdorffii) and Cravo (S. aromaticum) oils on the health and physiological quality of silk flower seeds (C. procera) harvested in the city of Tacima, PB. The treatments were constituted by Copa&iacute;ba (C. langsdorffii) and Cravo (S. aromaticum) essential oils at concentrations of 0.5; 1; 1.5; 2% and the fungicide Captan&reg; (240 g, i.a. 100 kg-1 seed). The control 0 (zero) corresponded only to the immersion of the seeds in distilled and sterilized water (ADE). In the evaluation of sanity, the method of incubation on filter paper (Blotter test) was performed, using twenty replicates of 10 seeds for each treatment. The physiological quality was evaluated by the germination test (G%), first germination count (FGC), germination speed index (GSI) and seedling dry mass (SDM). A microflora composed mainly of Alternaria sp. (52%), Fusarium sp. (70%), Helminthosporium sp. (40%), Cladosporium sp. (50%), Curvularia sp. (20%) and Nigrospora sp. (5%). The essential oils considerably reduced the percentage of fungi associated with silk flower seeds, but there was moderate phytotoxic effect under the germination and vigor of C. procera seeds.

scholarly journals In Vitro Antagonistic Effect of Gut Bacteriota Isolated from Indigenous Honey Bees and Essential Oils against Paenibacillus Larvae

2020 ◽  
Vol 21 (18) ◽  
pp. 6736 ◽  
Author(s):  
Miroslava Kačániová ◽  
Margarita Terentjeva ◽  
Jana Žiarovská ◽  
Przemysław Łukasz Kowalczewski
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Digestive Tract ◽  
Antagonistic Effect ◽  
Paenibacillus Larvae ◽  
Thymus Vulgaris ◽  
Plant Essential Oils ◽  
Microbial Groups ◽  
Selection Of

The aim of study was to isolate and identify the gut bacteria of Apis mellifera and to evaluate antagonistic effect of the bacteriota against Paenibacillus larvae, which causes American foulbrood (AFB) in honeybees. The dilution plating method was used for the quantification of selected microbial groups from digestive tract of bees, with an emphasis on the bacteriota of the bees’ intestines. Bacteria were identified using mass spectrometry (MALDI-TOF-MS Biotyper). Overall, five classes, 27 genera and 66 species of bacteria were identified. Genera Lactobacillus (10 species) and Bacillus (8 species) were the most abundant. Gram-negative bacteria were represented with 16 genera, whereas Gram-positive with 10 genera. Delftia acidovorans and Escherichia coli were the most abundant in the digestive tract of honey bee. Resistance to a selection of antimicrobials was assessed for the bacterial isolates from bee gut and confirmed against all antimicrobials included in the study, with the exception of cefepime. Lactobacillus spp., especially L. kunkeei, L. crispatus and L. acidophilus. showed the strongest antimicrobial activity against P. larvae, the causal pathogen of AFB. Antimicrobial activity of essential oils against isolated bacteria and two isolates of P. larvae were assessed. Application of a broad selection of plant essential oils indicated that Thymus vulgaris had the highest antimicrobial activity against P. larvae.

scholarly journals The Effect of Plant Oils for Reducing Contamination of Stored Packaged-Foodstuffs

2011 ◽  
Vol 51 (1) ◽  
pp. 82-86 ◽  
Author(s):  
Somaye Allahvaisi ◽  
Mostafa Maroufpoor ◽  
Arman Abdolmaleki ◽  
Saied-Ali Hoseini ◽  
Somaye Ghasemzadeh
Keyword(s):  
Essential Oils ◽  
Plant Oils ◽  
Syzygium Aromaticum ◽  
Stored Grain ◽  
Food Protection ◽  
Plant Essential Oils ◽  
Stored Product Pests ◽  
The Mean ◽  
Product Protection ◽  
Stored Grain Insects

The Effect of Plant Oils for Reducing Contamination of Stored Packaged-FoodstuffsA study to determine the effect of essential oils, used together with mixed packaging, on controlling stored-grain insects, was conducted in the laboratory. Plant essential oils are one of the materials that act like a contact-fumigant, offering the prospect for use in stored product protection. Plant essential oils must have the ability to repel the insects in order to prevent penetration to foodstuffs. The objective of the present study was to test the properties ofCinnamomum camphoraandSyzygium aromaticumfor preventing the penetration of pest insects, including:Sitotroga cerealellaandEphestia kuehniella5th instar larvae to packaged cereals. Ten grams of wheat and flour as foodstuffs were placed inside Cellophane packages and hung vertically in the top of a container. The repellent essential oils were used in the interior surface of containers. Each container had punctures at different parts and the insects were released around the container to determine insect penetration and the effect of the repellents. The experiments were conducted at 26±1°C, 60±5% RH in dark conditions. The highest concentration was 1.5 μl of essential oil per 0.5 ml acetone. Four days after the initiation of the experiment the results showedS. aromaticumhad more of a repellency effect thanC. camphoraon both pests that caused contamination deduction inside the packaged foodstuffs compared with the control. However, both the essential oils were effective as protectants of cereals butS. aromaticumwas more effective.S. aromaticumcaused the most repellency onE. kuehniella.The mean of repellency of all the concentrations was 81.4%. The results demonstrated the efficacy of these essential oils for use in organic food protection.C. camphoraandS. aromaticumcan prevent warehouse infestation of stored-product pests.

scholarly journals Effect of Plant Essential Oils against Rophalosiphum padi on Wheat and Barley

2017 ◽  
Vol 12 (9) ◽  
pp. 1934578X1701200
Author(s):  
Daniela Grul'ová ◽  
Silvia Mudrončeková ◽  
Valtcho D. Zheljazkov ◽  
Ivan Šalamon ◽  
Silvia I. Rondon
Keyword(s):  
Triticum Aestivum ◽  
Hordeum Vulgare ◽  
Essential Oils ◽  
Untreated Control ◽  
Rhopalosiphum Padi ◽  
Triticum Aestivum L ◽  
Thymus Vulgaris ◽  
Hordeum Vulgare L ◽  
Plant Essential Oils ◽  
Hyssopus Officinalis

The aim of the present study was to evaluate the effect of six EOs against Rhopalosiphum padi L. (Homoptera: Aphididae). This aphid is one of the most important cereal pests worldwide. EOs used in this study were from cumin ( Cuminum cyminum L.; Apiaceae), hyssop ( Hyssopus officinalis L.; Lamiaceae), costmary ( Tanacetum balsamita L. syn. Chrysanthemum balsamita L. Baill; Asteraceae), lovage ( Levisticum officinale W.D.J. Koch; Apiaceae), lavander ( Lavandula angustifolia Mill; Lamiaceae) and thyme ( Thymus vulgaris L.; Lamiaceae). Cultivar ORCF-10, a soft white winter wheat ( Triticum aestivum L.) cultivar and barley ( Hordeum vulgare L.) cv. ‘Alba’ were selected for the bioassays. Each EO was diluted with acetone (w/v) at different concentrations ranging from 0.1 % to 1.0 %. Number of R. padi landing on treated or untreated leaves was counted 24 h after the initial aphid transfer. In general, the repellency effect increased as the concentration of EOs increased regardless of treatments with the exception of lovage. Regardless of the substrate and treatment, lower numbers of aphids were observed on treated leaves at higher concentrations compared to the untreated control stressing the repellency effect. This study demonstrated that the EOs of cumin, hyssop, costmary, lavender, and thyme may have potential for aphids control.

scholarly journals Essential oils in the control of dry bubble disease in white button mushroom

2017 ◽  
Vol 47 (5) ◽  
Author(s):  
Tamara Leite dos Santos ◽  
Leônidas Leoni Belan ◽  
Diego Cunha Zied ◽  
Eustáquio Souza Dias ◽  
Eduardo Alves
Keyword(s):  
Essential Oils ◽  
Thymus Vulgaris ◽  
Good Growth ◽  
Syzygium Aromaticum ◽  
Cinnamon Oil ◽  
In Vivo Experiments ◽  
Dry Bubble ◽  
Dry Bubble Disease

ABSTRACT: Lecanicillium fungicola, which causes Dry bubble disease, induces infections and inflicts major losses in champignon production. The control can be managed through measures of hygiene and use of fungicides; however, in Brazil there are no registered products. This study aimed to estimate the influence of various essential oils extracted from Melissa officinalis, Thymus vulgaris, Origanum vulgare, Eucalyptus globulus, Cinnamomum zeylanicum and Syzygium aromaticum on the in vitro development and their uses. Therefore, analysis was performed of the L. fungicola isolates in vitro and the best oils were tested in vivo. Besides, the Agaricus bisporus - L. fungicola interaction was confirmed by scanning electron microscopy (SEM). Cinnamon and clove oils in concentrations of 0.4% and thyme oil of 0.8% were identified as good growth inhibitors of the pathogenic mycelium. Effective inhibition of the conidial germination was seen in all concentrations by cinnamon oil, and by clove and thyme oils only at 0.4% and 0.8%, respectively. When the essential oils were applied post-infestation in the in vivo experiments the incidence of the disease in the mushrooms was much lower. From the SEM it was clear that 19 hours after the inoculation of A. bisporus with L. fungicola, the spores had already completely germinated, revealing the presence of the infection. Therefore, the findings of this study indicated that the oil extracts of cinnamon, clove and thyme are potential and efficient alternatives in the control of dry bubble disease.

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