Rapid Depletion of Marbofloxacin Residues in Rabbit after Therapeutic Treatment

2005 ◽  
Vol 68 (11) ◽  
pp. 2480-2484 ◽  
Author(s):  
MATTEO LIGABUE ◽  
DARIO LUCCHETTI ◽  
TIZIANA CATONE ◽  
LAURA FABRIZI ◽  
LUIGI MARVASI ◽  
...  
Keyword(s):  
High Performance ◽  
Meat Production ◽  
Antibacterial Drug ◽  
Rabbit Muscle ◽  
Therapeutic Treatment ◽  
Antibacterial Drugs ◽  
Maximum Residue Level ◽  
Food Species ◽  
Liver And Kidney ◽  
A Minor

Although rabbit meat production represents a very small percentage of the world meat market, this percentage has been growing continuously during the last 30 years. Rabbit is considered a minor food species, and therefore no drugs are specifically registered for this animal. This situation encourages rabbit farmers to make off-label use of antibacterial drugs authorized for food-producing animal species other than rabbits. In the present study, the distribution and elimination of the fluoroquinolone antibacterial agent marbofloxacin in rabbit muscle, liver, and kidney was investigated. Marbofloxacin was chosen as a representative of a new generation of antibacterial drugs active against most gram-positive and gram-negative bacteria and mycoplasms; it is well tolerated and has short elimination times in bovine and swine species. Rabbits were treated with marbofloxacin at 2 mg kg of body weight−1 for 5 days. Residual concentrations in liver, kidney, and muscle tissues were determined posttreatment with high-performance liquid chromatography and fluorescence detection. Marbofloxacin was rapidly distributed and eliminated from rabbit tissues. Concentrations were higher in the liver and kidney than in muscle. However, 48 h after the end of treatment, marbofloxacin concentrations dropped below the maximum residue level fixed for this antibacterial drug in cattle and pigs. Considering the efficacy of marbofloxacin for the treatment of the most common rabbit diseases, its tolerability, and its short elimination time as verified in the present study, use of this antibacterial drug could be extended to therapeutic treatment of rabbits.

Residue Depletion Study of Danofloxacin in Cultured Tilapia (Oreochromis mossambicus)

2015 ◽  
Vol 98 (3) ◽  
pp. 575-579 ◽  
Author(s):  
Yang-Chi Fan ◽  
Shi-Yuan Sheu ◽  
Hong-Thih Lai ◽  
Ming-Huang Chang ◽  
Pei-Heng Chen ◽  
...  
Keyword(s):  
Body Weight ◽  
Single Dose ◽  
Oreochromis Mossambicus ◽  
Antibacterial Drug ◽  
Maximum Residue Level ◽  
Single Oral Administration ◽  
Residue Depletion ◽  
Liver And Kidney ◽  
Muscle Concentration ◽  
Fluoroquinolone Group

Abstract Danofloxacin is an antibacterial drug of the fluoroquinolone group developed for therapeutic purposes in veterinary medicine. The studies described here include investigations of the residues following a single dose or multiple doses of danofloxacin. Residue depletion studies were performed to determine residues in plasma and tissues of saltwater tilapia fish (Oreochromis mossambicus) after a single oral administration of danofloxacin at the dose of 10 mg/kg body weight and also after daily dose of 10 mg/kg body weight for five consecutive days. Danofloxacin residues were analyzed by HPLC with fluorescence detection. Following a single oral dose, danofloxacin residues in 6 h postdosing tilapia were at a maximum of 1.44, 12.48, and 13.18 μg/g in serum, liver, and kidney samples, respectively, while a peak muscle concentration of 2.15 μg/g was reached at 12 h. From single-dose data, the concentration of danofloxacin in serum, muscle, liver, and kidney samples declined with half-lives of 29, 34, 49, and 44 h, respectively. Based on the maximum residue level (MRL) of 0.1 μg/g in edible tissue for fin fish, the withdrawal times of danofloxacin in muscle were estimated to fall below the MRL after a withdrawal period of 21 days following the multiple-dose administration. These results may be helpful to regulatory agencies as they determine what tissues should be monitored to ensure that the established residue safety tolerance levels are not exceeded.

scholarly journals Impact of Commercial Oenotannin and Mannoprotein Products on the Chemical and Sensory Properties of Shiraz Wines Made from Sequentially Harvested Fruit

Foods ◽  
2018 ◽  
Vol 7 (12) ◽  
pp. 204 ◽  
Author(s):  
Sijing Li ◽  
Keren Bindon ◽  
Susan Bastian ◽  
Kerry Wilkinson
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Chemical Composition ◽  
High Performance ◽  
Red Wine ◽  
Descriptive Analysis ◽  
Sensory Properties ◽  
Fruit Maturity ◽  
A Minor ◽  
Early Harvest

The tannin and polysaccharide profiles and therefore sensory properties of wine are influenced by fruit maturity at harvest, and practices employed during winemaking. This study investigated the extent to which commercial winemaking supplements (skin and seed tannins, and mannoprotein (MP)) can enhance the mouthfeel properties of red wine, in particular, wine made from grapes harvested before commercial ripeness (early-harvest). Supplements were added to wines made from Shiraz grapes harvested at 20.8 and 24.5 °Brix. The chemical composition and mouthfeel properties of wines were then determined by high performance liquid chromatography and descriptive analysis (DA), respectively. Wines made from riper grapes had higher levels of tannin than wines made from early-harvest grapes, but similar polysaccharide levels were observed. The addition of seed oenotannin yielded higher tannin levels than addition of skin oenotannin, particularly for wines made from early-harvest grapes. The DA panel perceived sensory differences between H1 and H2 wines, but could not perceive any effect of supplementation on wine mouthfeel properties, with the exception of a minor increase in sweetness, attributed to mannoprotein addition to H1 wines, even when MP was added to wines at 2.5 times the level recommended for use in Australia.

scholarly journals Mitochondrial DNA of Nellore and European x Nellore crossing cattle of high performance

2007 ◽  
Vol 42 (8) ◽  
pp. 1203-1205 ◽  
Author(s):  
Rogério Abdallah Curi ◽  
Lígia Souza Lima Silveira da Mota ◽  
Antônio Carlos Silveira
Keyword(s):  
Mitochondrial Dna ◽  
High Frequency ◽  
High Performance ◽  
Bos Taurus ◽  
Meat Production ◽  
Bos Taurus Indicus ◽  
Nd5 Gene

The objective of this work was to evaluate, through a polymorphism in the ND5 gene of the bovine mitochondrial DNA, the frequency of Bos taurus indicus mtDNA individuals in a sample of Nellore purebred origin animals (n = 69) and crossbred animals originated from crosses of European sires and Nellore purebred origin females (n = 275). Only 2.26% (8/354) of the animals presented Bos taurus indicus mtDNA. The high frequency of Bos taurus taurus mtDNA in these animals can be a consequence of selection, once the animals studied are originated from selected lineages of high performance for meat production.

scholarly journals Морфологічні зміни тимусу курчат при різних схемах антибактеріальної терапії

2017 ◽  
Vol 19 (73) ◽  
pp. 11-18
Author(s):  
L.Y. Lyzogub
Keyword(s):  
Antibacterial Agents ◽  
Normal Development ◽  
Negative Impact ◽  
Preventive Treatment ◽  
Antibacterial Drug ◽  
Antibacterial Drugs ◽  
Main Thing ◽  
The Moment ◽  
The One ◽  
Positive Effect

The maximum number of products at the lowest cost – is the main thing, what modern poultry industry can be characterized. One of the most pressing problems at the moment is early antibiotic therapy and the associated safety of young poultry early age. In this regard, of particular interest for this study is how the most widely used antibacterial drugs effects on the one of key organs of the immune system – thymus. The article presents the data obtained in the course of the experiment, which determines how preventive treatment schemes, that includes usage single and multiple antibacterial drugs, influenced on the morphological status of the chickens thymus. We selected scheme and a combination of antibacterial drugs have not been studied previously. That was compared with the usage of probiotic Bolmol at the same treatment scheme. A comparison of morpho-functional state of the thymus of chickens 15-, 22- and 41-days-old, when applying several treatments schemes. The schemes of treatment included: the usage of one or two antibiotics during the cycle, compared with the usage of probiotic. We made the conclusions about the absence of the negative impact of selected drugs on the morphofunctional state of the thymus and the positive effect of probiotic «Bolmol». The morphometric parameters of the cortex and medulla in the lobules of the thymus of chicks 15-day-old, taking as an antibacterial drug probiotic Bolmol, were not significantly different from control values and were statistically significant when compared with chicks who used antibiotics: the width of the cortical area was on 15% and 29.09% wider then chickens receiving antibiotic treatment. This indicates a positive effect of probiotic Bolmol compared with antibacterial agents which were chosen. Thus, these data demonstrate that the use of probiotics in cyclic schemes of antibiotic prophylaxis contribute to the normal development of the thymus, which in turn leads to increased resistance and preservation of poultry. Was given the substantiation about safely usage of cyclic schemes of antibacterial drugs in the event of objective necessity.

scholarly journals Determination of the bifenazate insecticide in apples and cucumbers by high performance liquid chromatography

2019 ◽  
Vol 55 (4) ◽  
pp. 422-428
Author(s):  
M. F. Zayats ◽  
S. M. Leschev
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Diode Array ◽  
The European Union ◽  
Optimal Conditions ◽  
Plant Materials ◽  
Ultraviolet Detection ◽  
Maximum Residue Level ◽  
Distribution Constants

Based on the distribution constants of biphenazate, obtained experimentally and also calculated from literature data on the solubility of biphenazate in water and organic solvents, as well as experimental data on the extraction of biphenazate from plant matrices by various extractants, we selected the optimal conditions for extracting biphenazate from apples and cucumbers. The conditions for the purification of the extracts were also selected. Acetonitrile in the presence of ammonium sulfate and hexane was used for extraction of the pesticide. Purification of extracts of plant materials was carried out by partitioning between hexane and water-acetonitrile mixture. The samples obtained after this treatment were pure enough to determine the residual amounts of biphenazate in them at the maximum residue level determined in Belarus and the countries of the European Union, or lower using widespread liquid chromatography with diode-array (ultraviolet) detection.

Liquid-chromatographic measurement of p-aminobenzoic acid and its metabolites in serum.

1988 ◽  
Vol 34 (11) ◽  
pp. 2235-2238 ◽  
Author(s):  
L L Yung-Jato ◽  
P R Durie ◽  
S J Soldin
Keyword(s):  
High Performance ◽  
Internal Standard ◽  
High Performance Liquid Chromatographic ◽  
Peak Height ◽  
Individual Compound ◽  
Aminobenzoic Acid ◽  
Serum Samples ◽  
The Individual ◽  
A Minor ◽  
Liquid Chromatographic

Abstract This is a high-performance liquid-chromatographic method for measuring p-aminobenzoic acid (PABA) and its metabolites in plasma or serum. Samples are deproteinized, then extracted with organic solvents before chromatography. For quantification, the peak height of the individual compound is compared with that of the internal standard. Analytical recoveries ranged from 41% to 100%, depending on the compound studied. Comparison of patients' samples after oral administration of either N-benzoyl-L-tyrosyl-p-aminobenzoic acid or free PABA revealed that PABA is extensively metabolized and conjugated to either p-acetamidobenzoic acid, p-aminohippuric acid, or p-acetamidohippuric acid. PABA concentrations in serum as measured with the Bratton-Marshall ultraviolet spectrophotometric procedure would appear predominantly to reflect measurements of metabolites, with only a minor contribution from PABA itself.

scholarly journals Extraction of Phenolic Compounds from Tabernaemontana catharinensis Leaves and Their Effect on Oxidative Stress Markers in Diabetic Rats

Molecules ◽  
2020 ◽  
Vol 25 (10) ◽  
pp. 2391 ◽  
Author(s):  
Rafael Sari ◽  
Paula Conterno ◽  
Leticia Dangui da Silva ◽  
Vanderlei Aparecido de Lima ◽  
Tatiane Luiza Cadorin Oldoni ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Phenolic Compounds ◽  
High Performance ◽  
Principal Component ◽  
Antioxidant Defense System ◽  
Diabetic Rats ◽  
Total Phenolic ◽  
Antioxidant Enzyme Activities ◽  
Protein Thiols ◽  
Liver And Kidney

The aim of this study was to evaluate the most effective extraction condition (temperature, solvent type and time) for recovery of high-value phytochemicals present in the Tabernaemontana catharinensis leaves (TC) and to assess their effect on biochemical parameters in streptozotocin-induced diabetic rats. The extraction of phenolic compounds from TC using a factorial design (FD) 2³, high performance liquid chromatography (HPLC), response surface methodology (RSM) and principal component analysis (PCA) were studied. It was found that the optimal conditions for extraction of phenolics were higher temperature (65 °C) and time (60 min) using ethanol as extractor solvent. In this condition of extraction (A8), total phenolic compounds (TPC) and antioxidant activity (AA) were determined. Additionally, this extract was used to evaluate their effect on antioxidant enzyme activities (superoxide dismutase (SOD) and catalase (CAT)) as well as lipid peroxidation (LP) and protein thiols level (PSH) in the liver and kidneys of normal and diabetic rats. As result, T. catharinensis extract presented TPC content of 23.34 mg EAG/g (equivalent gallic acid) and AA of 34.26 μmol Trolox/g. Phenolic acids (ferulic acid and coumaric acid) and flavonoids (quercetin, rutin and pinocembrin) could be recovered and identified by HPLC. This study indicated an important role of the T. catharinensis extract on free radical inactivation and on the antioxidant defense system in diabetic rats. In fact, the use of T. catharinensis extract restored the normal activity of SOD (p < 0.05) and suppressed malondialdehyde levels in liver and kidney tissues. Thus, the T. catharinensis extract, rich in phenolic compounds, can be responsible for the recover the enzymatic changes in the liver and kidney tissues provoked by diabetes in rats. In addition, the lipid peroxidation rate decreased in the diabetic rats treated with T. catharinensis.

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