Antibacterial Efficiency of Spanish Satureja montana Essential Oil against Listeria monocytogenes among Natural Flora in Minced Pork

2008 ◽  
Vol 71 (3) ◽  
pp. 502-508 ◽  
Author(s):  
J. J. CARRAMIÑANA ◽  
C. ROTA ◽  
J. BURILLO ◽  
A. HERRERA
Keyword(s):  
Listeria Monocytogenes ◽  
Essential Oil ◽  
Synergistic Effects ◽  
Biological Activities ◽  
Thymus Vulgaris ◽  
Moderate Activity ◽  
Log Reduction ◽  
Natural Flora ◽  
Aerobic Flora ◽  
Satureja Montana

The purpose of this study was to examine the effectiveness of winter savory (Satureja montana) essential oil (EO) for control of growth and survival of experimentally inoculated Listeria monocytogenes serovar 4b (104 CFU/g) among natural flora in minced pork. EOs of French thyme (Thymus vulgaris F) and rosemary (Rosmarinus officinalis) cultivated in the same region of Aragon (northeastern Spain) were used as reference ingredients. The EOs obtained by hydrodistillation were added at concentrations of 0.25, 0.5, 1, and 2.5 μl/g (vol/wt), and the samples were kept at 4°C in air for up to 7 days. The populations of L. monocytogenes and total viable bacteria were determined in the control and treated samples at 0, 1, 3, 5, and 7 days. Moderate activity of S. montana EO against L. monocytogenes was observed (at 2.5 μl/g, reductions of 0.27 log CFU/g by day 3 and 0.61 log CFU/g by day 7), with higher activity against aerobic flora. The greatest reduction in aerobic flora was on day 3 (at 2.5 μl/g) from 1.10 to 1.45 log CFU/g. S. montana EO was comparable to T. vulgaris F EO in listericidal activity, but R. officinalis EO was ineffective against the L. monocytogenes and aerobic flora in the minced meat model. The ~3-log reduction in aerobic flora with T. vulgaris F EO at 0.25 to 2.5 μl/g after 5 days of storage was the most significant reduction. Depending on sensory considerations, the addition of active EOs in combination with other preservation techniques for synergistic effects may provide alternatives to synthetic chemical preservatives. Suggestions on relationships between chemical composition and biological activities of EOs are outlined.

Chemical composition, antimicrobial, antioxidative and anticholinesterase activity of Satureja Montana L. ssp montana essential oil

2014 ◽  
Vol 9 (7) ◽  
pp. 668-677 ◽  
Author(s):  
Tatjana Mihajilov-Krstev ◽  
Dragan Radnović ◽  
Dušanka Kitić ◽  
Vesna Jovanović ◽  
Violeta Mitić ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Human Serum ◽  
Biological Activities ◽  
Serum Cholinesterase ◽  
Health Improvement ◽  
Chemical Compositions ◽  
Bacterial Strains ◽  
Antimicrobial Potential ◽  
Satureja Montana

AbstractThe present study investigates the chemical compositions of three Satureja montana L. ssp montana essential oils and correlates chemical variability with biological activities. GC/MS analysis showed that with an increase in altitude (100–500–800 m), a higher content of linalool, terpinen-4-ol and cis-sabinene hydrate was found, while the percentage of phenolic compounds, thymol and carvacrol decreased. Antimicrobial activity of the essential oils was tested against 7 fungal and 23 bacterial strains. The essential oil characterized by the highest content of phenols and alcohols exhibited the highest antimicrobial potential. The correlation analysis showed that the major carriers of the obtained antioxidant activity are oxygenated monoterpenes. All essential oils inhibited human serum cholinesterase activity. High antimicrobial potential, together with moderate antioxidant capacity and strong inhibition of human serum cholinesterase, classifies S. montana essential oil as a natural source of compounds that can be used in the treatment of foodborne and neurological diseases, wound and other infections, as well as for general health improvement.

Antimicrobial Activity of Individual and Combined Essential Oils against Foodborne Pathogenic Bacteria

2016 ◽  
Vol 79 (2) ◽  
pp. 309-315 ◽  
Author(s):  
FATIMA REYES-JURADO ◽  
AURELIO LÓPEZ-MALO ◽  
ENRIQUE PALOU
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Inhibitory Concentration ◽  
Synergistic Effects ◽  
Gas Chromatography Mass Spectrometry ◽  
Thymus Vulgaris ◽  
Chemical Compositions ◽  
Fractional Inhibitory Concentration ◽  
Mustard Essential Oil

ABSTRACTThe antimicrobial activities of essential oils from Mexican oregano (Lippia berlandieri Schauer), mustard (Brassica nigra), and thyme (Thymus vulgaris) were evaluated alone and in binary combinations against Listeria monocytogenes, Staphylococcus aureus, or Salmonella Enteritidis. Chemical compositions of the essential oils were analyzed by gas chromatography–mass spectrometry. The MICs of the evaluated essential oils ranged from 0.05 to 0.50% (vol/vol). Mustard essential oil was the most effective, likely due to the presence of allyl isothiocyanate, identified as its major component. Furthermore, mustard essential oil exhibited synergistic effects when combined with either Mexican oregano or thyme essential oils (fractional inhibitory concentration indices of 0.75); an additive effect was obtained by combining thyme and Mexican oregano essential oils (fractional inhibitory concentration index = 1.00). These results suggest the potential of studied essential oil mixtures to inhibit microbial growth and preserve foods; however, their effect on sensory quality in selected foods compatible with their flavor needs to be assessed.

scholarly journals GC-MS analysis and biological activities of Thymus vulgaris and Mentha arvensis essential oil

2019 ◽  
Vol 44 (3) ◽  
pp. 388-396
Author(s):  
Ashfaq Ahmad Khan ◽  
Muhammad Shoaib Amjad ◽  
Saboon
Keyword(s):  
Antioxidant Activity ◽  
Essential Oil ◽  
Biological Activities ◽  
Colorimetric Method ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Thymus Vulgaris ◽  
Dpph Assay ◽  
Mentha Arvensis ◽  
Ms Analysis

Abstract Background Essential oils are chemical products produced by odoriferous glands from a variety of plants. These essential oil have many health benefits i.e. antiseptic, anti-inflammatory and antimicrobial activities. So due to these medicinal properties present study was designed to analyze essential oil of Thymus vulgaris and Mentha arvensis for their chemical composition and biological activities. Materials and methods Essential oil from these plants were extracted by hydrodistillation method, and analyzed by GC-MS. To test the microbial activity of these oil disk diffusion method and micro wells method were used. For free radical scavenging DPPH assay was used. However total phenolic content was measured by colorimetric method. Results The GC-MS analysis of T. vulgaris oil showed the presence of 47 chemical compounds among which thymol, terpinene, p-cymene and carvacrol were major. However essential oil of M. arvensis showed the presence of 28 constituents, among which the Menthone, Menthol, Isomenthone, Eucalyptol, neo-Menthol, cis-Piperitone oxide, Linalool, Thymol, Limonene, and α-Phellandrene were major. Essential oil from both these plant tested for antimicrobial activity showed that the T. vulgaris oil was effective against seven bacterial strains and the essential oil of M. arvensis was effective against six bacterial strain. The antioxidant activity of both samples by DPPH assay which showed positive result. Conclusion As both species showed the presence of active components, positive microbial activities, and antioxidant activity so, research should be carried on for further biological activities of these oil for betterment of living beings.

Synergistic Effects of Butyl Para-Hydroxybenzoate and Mild Heating on Foodborne Pathogenic Bacteria

2020 ◽  
Author(s):  
Zhujun Gao ◽  
Qiao Ding ◽  
Chongtao Ge ◽  
Robert C. Baker ◽  
Rohan V. Tikekar ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Pathogenic Bacteria ◽  
Thermal Inactivation ◽  
Heating Temperature ◽  
Synergistic Effects ◽  
Brain Heart Infusion ◽  
Similar Treatment ◽  
Log Reduction ◽  
Synergistic Enhancement ◽  
Foodborne Pathogenic Bacteria

ABSTRACT While high temperature heat treatments can efficiently reduce pathogen levels, they also affect the quality and nutritional profile of foods, as well as increase the cost of processing. The food additive butyl para-hydroxybenzoate (BPB) was investigated for its potential to synergistically enhance the thermal inactivation at mild heating temperatures (54 – 58 ºC). Four foodborne pathogenic bacteria, Cronobacter sakazakii , Salmonella enterica serotype Typhimurium, attenuated Escherichia coli O157:H7 and Listeria monocytogenes, were cultured to early stationary phase and then subjected to mild heating in a model food matrix (Brain Heart Infusion) containing low levels BPB (≤ 125 ppm). The heating temperature used with each bacterium was selected based on the temperature that would yield an approximate 1 – 2 log reduction over 15 min heating in BHI without BPB using a submerged coil apparatus. The inclusion of BPB at concentrations ≤ 125 ppm resulted in significant enhancement of thermal inactivation, achieving 5 – > 6 log reductions of the Gram-negative strains and D-values of < 100 sec. Listeria monocytogenes achieved at 3 – 4 log reduction with a similar treatment. No significant inactivation was noted in the absence of the mild heating for the same time period. This study provides an additional proof of concept that low temperature inactivation of foodborne pathogens can be realized by synergistic enhancement of thermal inactivation by food components that affect microbial cell membranes.

Characterization of Mexican Coriander (Eryngium foetidum) Essential Oil and Its Inactivation of Listeria monocytogenes In Vitro and during Mild Thermal Pasteurization of Pineapple Juice

2014 ◽  
Vol 77 (3) ◽  
pp. 435-443 ◽  
Author(s):  
JEAN J. ESSIA NGANG ◽  
MAXIMILIENNE A. NYEGUE ◽  
FOE C. NDOYE ◽  
ALEX D. TCHUENCHIEU KAMGAIN ◽  
SYLVAIN L. SADO KAMDEM ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Essential Oil ◽  
Thermal Inactivation ◽  
Inactivation Kinetics ◽  
Pineapple Juice ◽  
Log Reduction ◽  
Eryngium Foetidum ◽  
Time Required

The aim of this work was to characterize the essential oil (EO) of Eryngium foetidum (EfEO) and assess its activity toward Listeria monocytogenes in broth and during thermal inactivation of the pathogen in pineapple juice. In this respect, EfEO was chemically characterized, and its antilisteria potential in broth as a function of pH, cell load, and EfEO concentration was assessed through a central composite design. Furthermore, the inactivation kinetics of L. monocytogenes in the juice were assessed by combining EfEO and low pasteurization temperatures. A total of 81 compounds were identified from EfEO. The reduction of pH and cell load increased EO activity. The use of only 15 ppm of EfEO during pasteurization of pineapple juice at 60°C reduced the time required for a 4-log reduction in L. monocytogenes CFU/ml by 74.9% (i.e., from 8.5 to 2.1 min) compared with treatment without EfEO. It could be concluded that EfEO activity toward L. monocytogenes increases with the reduction of pH and that it can be used at sublethal concentrations in combination with low temperatures in pineapple juice pasteurization. This study demonstrates that EO-assisted pasteurization is a promising strategy for the reduction of thermal impact during juice production. EfEO is easily available and compatible with many juices and is thus promising for industrial application.

scholarly journals Comparative inhibitory effects of Thymus vulgaris L. essential oil against Staphylococcus aureus, Listeria monocytogenes and mesophilic starter co-culture in cheese-mimicking models

2015 ◽  
Vol 52 ◽  
pp. 59-65 ◽  
Author(s):  
Rayssa Julliane de Carvalho ◽  
Geanny Targino de Souza ◽  
Vanessa Gonçalves Honório ◽  
Jossana Pereira de Sousa ◽  
Maria Lúcia da Conceição ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Listeria Monocytogenes ◽  
Essential Oil ◽  
Thymus Vulgaris ◽  
Inhibitory Effects

scholarly journals The Potential Gastrointestinal Health Benefits of Thymus Vulgaris Essential Oil: A Review

2019 ◽  
Vol 12 (04) ◽  
pp. 1793-1799 ◽  
Author(s):  
Abdulaziz Almanea ◽  
Gamal S. Abd El-Aziz ◽  
Mohamed Morsi M. Ahmed
Keyword(s):  
Medicinal Plants ◽  
Essential Oil ◽  
Biological Activities ◽  
Food Additives ◽  
Health Benefits ◽  
Pharmaceutical Preparations ◽  
Flowering Plant ◽  
Thymus Vulgaris ◽  
Potential Health ◽  
Therapeutic Benefits

In recent times, medicinal plants have received great attention worldwide due to their effective pharmacological properties and therapeutic benefits. Numerous chemical compounds extracted from various medicinal plants have manifold biological activities. Thymus vulgaris (TV) is a flowering plant with an aromatic odor that has been broadly applied in conventional medicine, food additives, and phyto-pharmaceutical preparations. It is recognized to have promising therapeutic potential for curing various types of diseases. The essential oil extracted from TV, which contains a high quantity of flavonoids, possess antioxidant and antimicrobial activities. Consequently, it could be utilized as a good source in developing novel natural antioxidants and antibiotics. This review explores some of the potential health benefits of TV essential oil (TVEO) on the gastrointestinal tract.

scholarly journals Antioxidant, Antiproliferative and Antimicrobial Activities of the Volatile Oil from the Wild Pepper Piper capense Used in Cameroon as a Culinary Spice

2013 ◽  
Vol 8 (12) ◽  
pp. 1934578X1300801 ◽  
Author(s):  
Verlaine Woguem ◽  
Filippo Maggi ◽  
Hervet P. D. Fogang ◽  
Léon A. Tapondjou ◽  
Hilaire M. Womeni ◽  
...  
Keyword(s):  
Essential Oil ◽  
Tumor Cells ◽  
Biological Activities ◽  
Human Tumor ◽  
Breast Adenocarcinoma ◽  
Moderate Activity ◽  
Oil Composition ◽  
Human Tumor Cells ◽  
Agar Disc

Wild pepper (Piper capense L.f., Piperaceae) is a spice traditionally used in western Cameroon to make soups called ‘ Nkui’ and ‘ Nah poh’. In the present work, the essential oil hydrodistilled from fruits was analyzed by GC-FID and GC-MS, and for in vitro biological activities, namely cytotoxic, antioxidant and antimicrobial, by MTT, DPPH, ABTS and agar disc diffusion methods. The oil composition was dominated by monoterpene hydrocarbons (56.5%) responsible for the pepper odor, such as β-pinene (33.2%), sabinene (10.0%) and α-pinene (8.9%). The oil induced a concentration-dependent inhibitory effect on human tumor cells MDA-MB 231 (breast adenocarcinoma), A375 (malignant melanoma) and HCT116 (colon carcinoma), showing IC50 values of 26.3, 76.0 and 22.7 μg/ml, respectively. The oil showed total antioxidant activity with a Trolox equivalent antioxidant concentration (TEAC) value of 140 μmol/g. The essential oil of P. capense proved to be an effective scavenger of the ABTS+ radical, with an activity only about 30 times lower than that of Trolox. Moderate activity was observed against the Gram-positive species Staphylococcos aureus and Enterococcus faecalis, and the yeast Candida albicans. The notable inhibition of some human tumor cells is worthy of further investigation to discover the possible mechanisms of action responsible for the observed cytotoxic effect of this essential oil.

scholarly journals Evaluation of the synergistic effects of antioxidant activity on mixtures of the essential oil from Apium graveolens L., Thymus vulgaris L. and Coriandrum sativum L. using simplex-lattice design

Heliyon ◽  
2019 ◽  
Vol 5 (6) ◽  
pp. e01942 ◽  
Author(s):  
Yasiel Arteaga Crespo ◽  
Luis Ramón Bravo Sánchez ◽  
Yudel García Quintana ◽  
Andrea Silvana Tapuy Cabrera ◽  
Abdel Bermúdez del Sol ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Essential Oil ◽  
Synergistic Effects ◽  
Coriandrum Sativum ◽  
Apium Graveolens ◽  
Thymus Vulgaris ◽  
Lattice Design ◽  
Simplex Lattice Design ◽  
Simplex Lattice

scholarly journals Biosynthesis of Lemongrass Essential Oil and the Underlying Mechanism for Its Insecticidal Activity

2020 ◽  
Author(s):  
Mohammad Mukarram ◽  
M. Masroor A. Khan ◽  
Andleeb Zehra ◽  
Sadaf Choudhary ◽  
Tariq Aftab ◽  
...  
Keyword(s):  
Essential Oil ◽  
Synergistic Effects ◽  
Biological Activities ◽  
Perennial Grass ◽  
Underlying Mechanism ◽  
Cymbopogon Flexuosus ◽  
Anti Cancer ◽  
Lemongrass Oil ◽  
Cytotoxic Responses ◽  
Lemongrass Essential Oil

Lemongrass (Cymbopogon flexuosus) is an aromatic perennial grass grown extensively for its essential oil. Lemongrass oil is chiefly a mixture of various cyclic and acyclic bioactive monoterpenes. We reviewed lemongrass oil and its biosynthesis in the present chapter along with its biochemical composition. Furthermore, we attempted to explore both the possible routes for essential oil biosynthesis in lemongrass, i.e. mevalonate and non-mevalonate pathways and how these pathways interwind with each other. Lemongrass oil has high commercial potential in medicinal, cosmetic, food and energy industries. Regarding the pharmacological properties, a wide array of biological activities has been observed in lemongrass oil such as antimicrobial, insecticidal, analgesic and anti-cancer properties as well as its efficacy as insect-repellent. The later sections were dedicated for the analysis of insecticidal property of the lemongrass oil and the mechanism working behind this phenomenon where it was observed that in addition to synergistic effects, various components of lemongrass oil can also induce specific neurotoxic and cytotoxic responses in the insects.

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