Survival of Helicobacter pylori in Turkish Fermented Sucuk and Heat-Treated Sucuk during Production

2011 ◽  
Vol 74 (12) ◽  
pp. 2055-2061 ◽  
Author(s):  
AHMET GUNER ◽  
KURSAT KAV ◽  
KEMAL KAAN TEKINSEN ◽  
YUSUF DOGRUER ◽  
NIHAT TELLI
Keyword(s):  
Heat Treatment ◽  
Helicobacter Pylori ◽  
Fermented Sausage ◽  
Appreciable Change ◽  
Group Iii ◽  
Log Reduction ◽  
Group I ◽  
Heat Treated ◽  
H Pylori ◽  
Traditional Fermentation

The aim of this study was to investigate the survival of Helicobacter pylori during production of sucuk (Turkish fermented sausage). The sucuk mixture was inoculated with H. pylori ATCC 43504 to produce a final level in the mixture of ~5 × 106 CFU/g. Samples in group I were fermented and dried traditionally at 22°C for 7 days. Samples in groups II and III were subjected to the traditional fermentation at 22°C for 3 days. After fermentation, group II samples were fermented and dried at 35°C for 4 days and group III samples were treated with heat until the core temperature reached 65°C. On the first day of fermentation, a 1-log reduction in H. pylori was found in all groups. The H. pylori levels in all groups increased by about 1 log CFU/g by the third day of fermentation and reached the inoculation level. On the fifth and seventh days of fermentation, no appreciable change occurred in the level of H. pylori in groups I and II. After heat treatment, the H. pylori levels were below the level of detection. These results suggest that H. pylori can grow during sucuk fermentation and that a heat treatment should be used during sucuk processing to destroy H. pylori.

scholarly journals A Clinical and Microbiological Evaluation of Helicobacter pylori in Recurrent Aphthous Stomatitis

2017 ◽  
Vol 18 (12) ◽  
pp. 1194-1197
Author(s):  
Kamala Rajendra ◽  
Sadananda M Purnachandra ◽  
Parthkumar C Patel ◽  
Jochima Cota ◽  
Venkat R Singh ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Clinical Examination ◽  
Recurrent Aphthous Stomatitis ◽  
Aphthous Stomatitis ◽  
Gastric Ulcers ◽  
Control Group ◽  
Group Iii ◽  
Group I ◽  
The Social ◽  
H Pylori

ABSTRACT Aim The aim of this study is to evaluate the association of Helicobacter pylori in recurrent aphthous stomatitis (RAS) with or without peptic ulcer. Materials and methods A total of 42 patients were selected for the present study from outpatient Department of Oral Medicine, and divided into control (group I) and test groups (groups II, III). A total number of 15 patients suffering from RAS based on history and clinical examination was selected as group II. A total number of 12 patients suffering concomitantly from RAS and duodenal ulcer based on history and clinical examination were selected as group III. A total number of 15 healthy subjects were selected as controls in group I. All selected subjects belonged to the age group of 16 to 45 years. Data were tabulated and statistically analyzed with Statistical Package for the Social Sciences (SPSS) statistical software version 19 of IBM, Chicago, Illinois, USA. Results Among all three groups, 54.8% was negative and 45.2% was positive for H. pylori. No significant relation was established between H. pylori and RAS. Conclusion In the present study, there was no association of H. pylori in patients with RAS and in patients with RAS along with gastric ulcers. Clinical significance This clinical study evaluated the relation of H. pylori in RAS patients. How to cite this article Rajendra K, Purnachandra SM, Patel PC, Cota J, Singh VR, Vatsal A. A Clinical and Microbiological Evaluation of Helicobacter pylori in Recurrent Aphthous Stomatitis. J Contemp Dent Pract 2017;18(12):1194-1197.

scholarly journals Can probiotics improve efficiency and safety profile of triple Helicobacter pylori eradication therapy? A prospective randomized study

2016 ◽  
Vol 73 (11) ◽  
pp. 1044-1049 ◽  
Author(s):  
Sasa Grgov ◽  
Tomislav Tasic ◽  
Biljana Radovanovic-Dinic ◽  
Daniela Benedeto-Stojanov
Keyword(s):  
Helicobacter Pylori ◽  
Randomized Study ◽  
Eradication Therapy ◽  
Saccharomyces Boulardii ◽  
Rapid Urease Test ◽  
Group I ◽  
Urease Test ◽  
Probiotic Strains ◽  
The Difference ◽  
H Pylori

Background/Aim. Some studies suggest the benefit of applying different probiotic strains in combination with antibiotics in the eradication of Helicobacter pylori (H. pylori) infection. The aim of this study was to evaluate the effect of co-administration of multiple probiotic strains with triple H. pylori eradication therapy. Methods. This prospective study included 167 patients with dyspeptic symptoms and chronic gastritis who were diagnosed with H. pylori infection and randomized into two groups. The group I of 77 patients underwent triple eradication therapy, for 7 days, with lansoprazole, 2 ? 30 mg half an hour before the meal, amoxicillin 2 ? 1.000 mg per 12 hours and clarithromycin 2 ? 500 mg per 12 hours. After the 7th day of the therapy, lansoprazole continued at a dose of 30 mg for half an hour before breakfast for 4 weeks. The group II of 90 patients received the same treatment as the patients of the group I, with the addition of the probiotic cultures in the form of a capsule comprising Lactobacillus Rosell-52, Lactobacillus Rosell-11, Bifidobacterium Rosell-1755 and Saccharomyces boulardii, since the beginning of eradication for 4 weeks. Eradication of H. pylori infection control was performed 8 weeks after the therapy by rapid urease test and histopathologic evaluation of endoscopic biopsies or by stool antigen test for H. pylori. Results. Eradication of H. pylori infection was achieved in 93.3% of the patients who received probiotics with eradication therapy and in 81.8% of patients who were only on eradication therapy without probiotics. The difference in eradication success was statistically significant, (p < 0.05). The incidence of adverse effects of eradication therapy was higher in the group of patients who were not on probiotic (28.6%) than in the group that received probiotic (17.7%), but the difference was not statistically significant. Conclusion. Multiple probiotic strains addition to triple eradication therapy of H. pylori achieves a significantly better eradication success, with fewer side effects of antibiotics.

scholarly journals Are Synbiotics added to the Standard Therapy to eradicate Helicobacter pylori in Children Beneficial? A Randomized Controlled Study

2017 ◽  
Vol 7 (1) ◽  
pp. 17-22 ◽  
Author(s):  
Banu N Şirvan ◽  
Merve K Usta ◽  
Nuray U Kızılkan ◽  
Nafiye Urgancı
Keyword(s):  
Helicobacter Pylori ◽  
Abdominal Pain ◽  
Triple Therapy ◽  
Standard Therapy ◽  
Randomized Controlled Study ◽  
Controlled Study ◽  
Randomized Controlled ◽  
Group I ◽  
Dyspeptic Symptoms ◽  
H Pylori

ABSTRACT Aim We aimed to evaluate the role of the addition of Bifidobacterium lactis-containing synbiotic to the triple therapy in the case of Helicobacter pylori eradication, the dyspeptic symptoms, and reducing the side effects of antibiotics. Materials and methods A total of 104 children aged between 5 and 17 years, who were histopathologically diagnosed with H. pylori were enrolled in this study, of whom 100 were included in the analysis. Patients were randomly classified into two groups. In the first group, 50 patients were administered amoxicillin + clarithromycin + lansoprazole for 14 days and B. lactis-containing synbiotic. In the second group, 50 patients were treated with the standard triple therapy. All patients were given information after completion of therapy. Results H. pylori eradication was achieved in 88% in group I who received standard therapy with additional synbiotic and 72% in group II (p = 0.046). The number of patients in the second group who suffered from abdominal pain between the 3rd and 14th day of the treatment was higher (p < 0.05). The addition of probiotics to the triple therapy significantly reduced the frequency of diarrhea, but no significant difference was detected in the frequency of metallic taste (p = 0.04, p = 0.418 respectively). Conclusion The addition of synbiotic to the triple therapy is effective for eradicating H. pylori infection in children and is usually helpful to reduce or eliminate dyspeptic symptoms like abdominal pain, diarrhea, and vomiting. This study suggest that improved tolerance to the eradication treatment also reduces the treatment failure by adding probiotics and encourages the future study using probiotic supplementation in H. pylori treatment. How to cite this article Şirvan BN, Usta MK, Kızılkan NU, Urgancı N. Are Synbiotics added to the Standard Therapy to eradicate Helicobacter Pylori in Children Beneficial? A Randomized Controlled Study. Euroasian J Hepato-Gastroenterol 2017;7(1):17-22.

scholarly journals Prediction of Epitopes in the Proteome of Helicobacter pylori

2018 ◽  
Vol 10 (7) ◽  
pp. 148 ◽  
Author(s):  
Elkin Navarro-Quiroz ◽  
Roberto Navarro-Quiroz ◽  
Pierine España-Puccini ◽  
José Luis Villarreal ◽  
Anderson Díaz Perez ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
World Health ◽  
Phase Variable ◽  
Human Pathogens ◽  
Web Tool ◽  
Group I ◽  
Increased Risk ◽  
Risk Of Disease ◽  
H Pylori ◽  
Health Organization

Helicobacter pylori (H. pylori) is classified by the World Health Organization (WHO) as a group I carcinogen and is one of the most efficient human pathogens with over half of the world's population colonized by this gram-negative spiral bacterium. H. pylori can cause a chronic infection in the stomach during early childhood that persists throughout life due to diverse mechanisms of immune response evasion. H. pylori has several factors strongly associated with increased risk of disease such as toxins, adhesins, and chemoattractants, some of which are highly polymorphic, phase variable, and have different functions. Conventional treatments involve the use of antibiotics. However, treatment frequently fails due to the resistance H. pylori has progressively developed to antibiotics. This creates the need for different treatments made possible by identifying new therapeutic targets in the pathogen’s genome.The purpose of this study was an in silico prediction of T- and B- epitopes in H. pylori proteins. Twenty-two external membrane proteins from H. pylori Strain 26695 (accession number NC_000915) were identified using the web tool Vaxign (http://www.violinet.org/vaxign/). A total of one-hundred epitopes (60 class I epitopes and 40 class II epitopes) that could be used to develop novel non-antibiotics drugs for an H. pylori infection were predicted.

scholarly journals Identification of Desmoglein-2 as a novel target of Helicobacter pylori HtrA in epithelial cells

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Sabine Bernegger ◽  
Robert Vidmar ◽  
Marko Fonovic ◽  
Gernot Posselt ◽  
Boris Turk ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Epithelial Cells ◽  
Intercellular Junctions ◽  
Matrix Metalloproteases ◽  
Proteomic Profiling ◽  
Proteomic Approach ◽  
Group I ◽  
H Pylori ◽  
Intercellular Adhesions

Abstract Background High temperature requirement A (HtrA) is an active serine protease secreted by the group-I carcinogen Helicobacter pylori (H. pylori). The human cell adhesion protein and tumor suppressor E-cadherin (hCdh1) expressed on the surface of gastric epithelial cells was identified as the first HtrA substrate. HtrA-mediated hCdh1 cleavage and subsequent disruption of intercellular adhesions are considered as important steps in H. pylori pathogenesis. In this study, we performed a proteomic profiling of H. pylori HtrA (HpHtrA) to decipher the complex mechanism of H. pylori interference with the epithelial barrier integrity. Results Using a proteomic approach we identified human desmoglein-2 (hDsg2), neuropilin-1, ephrin-B2, and semaphorin-4D as novel extracellular HpHtrA substrates and confirmed the well characterized target hCdh1. HpHtrA-mediated hDsg2 cleavage was further analyzed by in vitro cleavage assays using recombinant proteins. In infection experiments, we demonstrated hDsg2 shedding from H. pylori-colonized MKN28 and NCI-N87 cells independently of pathogen-induced matrix-metalloproteases or ADAM10 and ADAM17. Conclusions Characterizing the substrate specificity of HpHtrA revealed efficient hDsg2 cleavage underlining the importance of HpHtrA in opening intercellular junctions.

scholarly journals FIBRINOLYTIC AND PROTEOLYTIC ACTIVITY OF BLOOD PLASMA IN PEPTIC ULCER OF THE STOMACH, TAKING INTO ACCOUNT THE PATHOGENIC STRAINS OF HELICOBACTER PYLORI

2020 ◽  
Vol 8 (1) ◽  
pp. 1-7
Author(s):  
L. M. Honcharuk ◽  
O. I. Fediv ◽  
V. T. Kulachek ◽  
Y. M. Teleki
Keyword(s):  
Helicobacter Pylori ◽  
Peptic Ulcer ◽  
Blood Plasma ◽  
Proteolytic Activity ◽  
Fibrinolytic Activity ◽  
Control Group ◽  
Group Iii ◽  
Group I ◽  
Group Ii ◽  
Cag A

The purpose of the study is to investigate changes in fibrinolytic and proteolytic activity of blood plasma in patients with peptic ulcer (PU) taking into account pathogenic Helicobacter pylori (Hp) strains. Materials and methods. 93 patients with PU were examined, of which 30 patients with PU and concomitant Hp cag cag A+/vac A+ (group I), 31 patients with PU and concomitant Hp cag A-/vac A- (group II), 32 patients with PU without concomitant HP infection (group III). The control group consisted of 30 healthy individuals. Fibrinolytic activity of blood plasma was investigated with the help of lysis of azofibrin (fibrin associated with the azo dye orange), which in the alkaline medium turns a bright red color. The level of total (ТFA), enzymatic (FFA) and non-enzymatic fibrinolytic activity (NFA) was evaluated. Proteolytic activity of blood plasma was determined by the lysis of azoalbumin, azocasein and azokol. Research results. The study of fibrinolytic activity of blood plasma showed that the total fibrinolytic activity of blood plasma (TFA) in all groups was significantly higher compared to the control indicators: in patients of group I by 61.5 %, in patients by 40.9 %, in patients of group III by 30.3 %, with a significant intergroup difference between the groups. The growth of TFA was mainly due to FFA. In patients of group I, FFA increased by 2.06 times (p < 0.05), and in patients of group II – by 1.79 times (p < 0.05), in patients of group IIІ – by 1.52 times (p < 0.05) compared with the control. In patients with group I, FFA increased by 12.5 % ​​(p < 0.05) compared with group II. In all patients examined, there was an increase in the proteolytic activity of blood plasma, in particular in group I, the lysis of azoalbumin, azocasein and azocolol increased significantly 2.94 times, 2.83 times and 1.90 times, respectively, and in the patients of group II the investigated indicators increased accordingly 1.87-fold (p < 0.05), 1.96-fold (p < 0.05) and 1.40-fold (p < 0.05), in patients of group III, respectively 1.55 times (p < 0.05), 1.59 times (p < 0.05) and 1.18 times, compared to these values ​​in almost healthy subjects. Significantly more significant changes in proteolysis were detected in the presence of pathogenic Hp strains. Conclusion. Increased proteolytic and fibrinolytic activity of blood plasma is observed in patients with PU. The presence of concomitant Hp in PU leads to more pronounced changes in proteolysis and fibrinolysis. Pathogenic strains of Hp cag cag A+/vac A+ cause significantly more abnormalities in hemostasis.

scholarly journals The usefulness of melatonin supplementation in postmenopausal women with Helicobacter pylori-associated dyspepsia

2020 ◽  
Author(s):  
Cezary Chojnacki ◽  
Marta Mędrek-Socha ◽  
Paulina Konrad ◽  
Jan Chojnacki ◽  
Aleksandra Błońska
Keyword(s):  
Serum Level ◽  
Postmenopausal Women ◽  
Epigastric Pain ◽  
Serum Levels ◽  
Duodenal Mucosa ◽  
Urinary Concentration ◽  
Group Iii ◽  
Group I ◽  
Group Ii ◽  
H Pylori

Abstract Background Dyspeptic syndrome in the form of epigastric pain are particularly frequent in postmenopausal women. The aim of the study was to assess the role of melatonin in chronic dyspepsia in this group of women, with consideration of Helicobacter infection.Methods The study comprised 152 subjects Including 30 healthy women (group I), 60 women with asymptomatic H.pylori infection (group II), and 64 women H. pylori infected with chronic dyspepsia (group III). Endoscopic examination, histological assessment of gastric end duodenal mucosa, urease breath test (UBT-13C), and serum levels of 17-β-estradiol, follicle stimulating hormone, melatonin and urinary concentration of 6-sulfatoxymelatonin were determined by immunoenzymatic method. In group III – 14-day antibacterial treatment was introduced with pantoprazole, amoxicillin and levofloxacin. Afterward, in 32 women was administered placebo(group IIIa), and in 32 women (group IIIb) melatonin at a dose 1 m/morning and 3 mg/at bedtime, for six months.Results No significant differences were assessed between serum level of female hormone. Serum level of melatonin in group I and in group II was similar – 12,5 ± 2,72pg/ml and 10,5±3,73 pg/ml (p>0,05), whereas in group III was lesser – 5,72±1,42 pg/ml (p<0,001). Eradication of H.pylori was obtained in 75,0% women in group IIIa, and in 84,3% in group IIIb (p>0,05). After 6 months dyspeptic symptoms resolved in 43,7% patients in group IIIa and in 84,3% in group IIIb (p<0,001). Conclusion Melatonin supplementation is useful to complex therapy of H. pylori-associated dyspepsia, particularly in postmenopausal women at whom secretion of this hormone is decreased.

cagA-Positive Helicobacter pylori Populations in China and The Netherlands Are Distinct

1998 ◽  
Vol 66 (5) ◽  
pp. 1822-1826 ◽  
Author(s):  
Arie van der Ende ◽  
Zhi-Jun Pan ◽  
Aldert Bart ◽  
René W. M. van der Hulst ◽  
Monique Feller ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
The Netherlands ◽  
Random Amplified Polymorphic Dna ◽  
Chromosomal Dna ◽  
High Confidence ◽  
Protein Levels ◽  
Group I ◽  
Rapd Pcr ◽  
H Pylori ◽  
Chinese Group

ABSTRACT The aim of this research was to study whether and to what extent Chinese cagA-positive Helicobacter pyloriisolates differ from those in The Netherlands. Analysis of random amplified polymorphic DNA (RAPD)-PCR-assessed DNA fingerprints of chromosomal DNA of 24 cagA-positive H. pylori isolates from Dutch (n = 12) and Chinese (n = 10) patients yielded the absence of clustering. Based on comparison of the sequence of a 243-nucleotide part ofcagA, the Dutch (group I) and Chinese (group II)H. pylori isolates formed two separate branches with high confidence limits in the phylogenetic tree. These two clusters were not observed when the sequence of a 240-bp part ofglmM was used in the comparison. The number of nonsynonymous substitutions was much higher in cagA than inglmM, indicating positive selection. The average levels of divergence of cagA at the nucleotide and protein levels between group I and II isolates were found to be high, 13.3 and 17.9%, respectively. Possibly, the pathogenicity island (PAI) that has been integrated into the chromosome of the ancestor of H. pylori now circulating in China contained a differentcagA than the PAI that has been integrated into the chromosome of the ancestor of H. pylori now circulating in The Netherlands. We conclude that in China and The Netherlands, two distinct cagA-positive H. pylori populations are circulating.

Dissecting the Helicobacter pylori-regulated transcriptome of B cells

2020 ◽  
Vol 78 (7) ◽  
Author(s):  
Bianca E Chichirau ◽  
Tamara Scheidt ◽  
Sebastian Diechler ◽  
Theresa Neuper ◽  
Jutta Horejs-Hoeck ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Immune System ◽  
B Cells ◽  
Mhc Class Ii ◽  
Molecular Mechanisms ◽  
Inflammatory Responses ◽  
Group I ◽  
Cytotoxin Associated Gene A ◽  
And Migration ◽  
H Pylori

ABSTRACT Persistent infections with the bacterial group-I carcinogen Helicobacter pylori (H. pylori) have been associated with a broad range of gastric disorders, including gastritis, ulceration, gastric cancer or mucosa-associated lymphoid tissue (MALT) lymphoma. Pathogenesis of H. pylori requires a balance between immune tolerance and defense. Although H. pylori induces inflammatory responses, the immune system cannot eliminate the pathogen. The detailed molecular mechanisms of how H. pylori interferes with cells of the immune system, in particular infiltrated B cells, are not well investigated. Previously, it was shown that the bacterial effector and oncoprotein cytotoxin-associated gene A (CagA) is delivered into B cells followed by its tyrosine-phosphorylation. To investigate the functional consequences in B cells colonized by CagA-positive H. pylori, we analyzed the global transcriptome of H. pylori-infected Mec-1 cells by RNA sequencing. We found 889 differentially expressed genes (DEGs) and validated JUN, FOSL2, HSPA1B, SRC, CXCR3, TLR-4, TNF-α, CXCL8, CCL2, CCL4, MHC class I and MHC class II molecules by qPCR, western blot, flow cytometry and ELISA assays. The H. pylori-specific mRNA expression signature reveals a downregulation of inflammation- and migration-associated genes, whereas central signal transduction regulators of cell survival and death are upregulated.

scholarly journals Hospital infections in Spain. I.Staphylococcus aureus(1978–91)

1993 ◽  
Vol 110 (3) ◽  
pp. 533-542 ◽  
Author(s):  
A. Vindel ◽  
P. Trincado ◽  
MM. Martin De Nicolas ◽  
E. Gomez ◽  
C. Martin Bourgon ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Staphylococcus Aureus ◽  
Considerable Increase ◽  
Hospital Infections ◽  
Phage Group ◽  
Group Iii ◽  
The Past ◽  
Phage Types ◽  
Group I ◽  
Sporadic Cases

SUMMARYThis study was undertaken to determine the distribution of phage types ofStaphylococcus aureusisolates from hospital outbreaks or sporadic cases received in our laboratory during the past 14 years. The records for 15803 isolates from 55 Spanish hospitals have been analysed.In relation to sporadic isolates we have been able to detect the predominance of phage group I and non-typable staphylococcal strains. Since 1989, we have observed a considerable increase in hospital infection caused by methicillin-resistantS. aureusstrains which we could differentiate in to two groups; one belonging to phage group III (6/47/54/75/77/84/85) and other groups of non-typable strains which could be classified as phage group I-III after heat treatment (29/77/84) and with similar patterns by reverse typing (6/47/53/54/75/83A/84/85/W57/1030/18042).During 1990 and 1991, these strains have extended widely to at least six different autonomous regions creating an epidemic situation in Spain.

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