Phenotypic and Genotypic Characterization of Salmonella spp. Isolated from Foods Involved in Human Salmonellosis Outbreaks in Minas Gerais State, Brazil

2021 ◽  
Author(s):  
Leandro Leão Faula ◽  
MONICA M.O.P. CERQUEIRA ◽  
JOVITA E.G.C. MADEIRA ◽  
MARCELO R. SOUZA ◽  
FERNANDO N. SOUZA ◽  
...  
Keyword(s):  
Virulence Genes ◽  
Multidrug Resistant ◽  
Minas Gerais ◽  
Health Centers ◽  
Salmonella Spp ◽  
Resistant Phenotype ◽  
Minas Gerais State ◽  
Human Salmonellosis ◽  
Human Infections

Salmonella spp. is one of the primary pathogens that cause foodborne diseases worldwide. In the present study, we deeply characterized Salmonella spp. originated from foods related to human salmonellosis outbreaks in Minas Gerais – Brazil, from 2003 to 2017. In this regard, the serotyping, antimicrobial susceptibility, virulence genes and genetic polymorphism determined by rep-PCR were performed in 70 Salmonella spp. isolates. Thirteen serotypes of Salmonella spp. were identified, and S. Enteritidis and S. Typhimurium were the most prevalent, both corresponding to 74.3% (52/70) of all isolates. Sixty-five (92.8%) isolates demonstrated to be resistant to at least one of the 15 antimicrobials tested. Ten isolates (14.2%) showed a multidrug-resistant phenotype. Sixteen virulence genes were screened and detection ranged from 75.7 to 100% of all isolates. There was a statistical difference among Salmonella spp. serotypes in detecting the sipB , sopE , lfpA , sefA and spvC genes. Forty isolates of S. Enteritidis from 16 outbreaks were grouped into 14 fingerprints, while 12 S. Typhimurium retrieved six fingerprints. The bacteria presented a serological pattern like those reported by main public health centers worldwide. One major concern of our findings is the high levels of both detection of virulence genes and resistance to antimicrobials, especially to critically important drugs. In this concern, especial attention should be given to the serotype S. Enteritidis. Although the isolates of Salmonella spp. have presented a relatively variable genome, high genetic similarity was observed among them, with some of them sharing identical fingerprints. These results corroborate the hypothesis of clonal circulation of Salmonella spp. in human infections in Minas Gerais.

scholarly journals Antimicrobial susceptibility and molecular characterization of Salmonella serovar Ndolo isolated from outbreaks in cattle and horses

2018 ◽  
Vol 48 (12) ◽  
Author(s):  
Carolina Pantuzza Ramos ◽  
Rafael Gariglio Clark Xavier ◽  
Carlos Augusto Gomes Leal ◽  
Elias Jorge Facury Filho ◽  
Antonio Ultimo de Carvalho ◽  
...  
Keyword(s):  
Clavulanic Acid ◽  
Antimicrobial Susceptibility ◽  
Virulence Genes ◽  
Susceptibility Testing ◽  
Domestic Animals ◽  
Amoxicillin Clavulanic Acid ◽  
Resistant Strains ◽  
Human Infections ◽  
First Time

ABSTRACT: The present study aimed to describe and characterize, for the first time, two outbreaks of salmonellosis caused by Salmonella Ndolo in foals and calves in Brazil and compare the isolated strains with S. Ndolo previously identified in asymptomatic reptiles. The affected calves and foals presented fever, lethargy, and profuse diarrhea. Isolated strains were subjected to antimicrobial susceptibility testing, characterized according to virulence genes, and fingerprinted by ERIC-PCR. Salmonella Ndolo was identified in fecal samples from two foals and four calves. One isolate from a calf was resistant to amoxicillin/clavulanic acid, trimethoprim/sulfamethoxazole, and florfenicol. Strains from two other calves were resistant to oxytetracycline. All virulence genes tested were present in the isolates, and two major clusters of closely related strains were identified by ERIC-PCR, each per outbreak. This is the first report of Salmonella Ndolo infection in domestic and symptomatic animals. Previously, this serovar had been identified only in human infections. The presence of relevant virulence genes in all Salmonella Ndolo isolates and the detection of antimicrobial multi-resistant strains highlighted the importance of monitoring serovars associated with salmonellosis in domestic animals.

Multivariate geostatistical application for climate characterization of Minas Gerais State, Brazil

2010 ◽  
Vol 102 (3-4) ◽  
pp. 417-428 ◽  
Author(s):  
Luiz G. de Carvalho ◽  
Marcelo de Carvalho Alves ◽  
Marcelo S. de Oliveira ◽  
Rubens L. Vianello ◽  
Gilberto C. Sediyama ◽  
...  
Keyword(s):  
Minas Gerais ◽  
Minas Gerais State

Molecular detection of biofilm, virulence and antimicrobial resistance associated genes of Salmonella serovars isolated from pig and chicken of Mizoram, India

2019 ◽  
Author(s):  
S. Chakraborty ◽  
P. Roychoudhury ◽  
I. Samanta ◽  
P. K. Subudhi ◽  
Lalhruaipuii Lalhruaipuii ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Virulence Genes ◽  
Antimicrobial Agents ◽  
Virulence Gene ◽  
Microtiter Plate ◽  
Multidrug Resistant ◽  
Salmonella Spp ◽  
Microtiter Plate Assay ◽  
Antimicrobial Sensitivity ◽  
Salmonella Infections

Salmonella has emerged as one of the most important food-borne pathogens for humans as well as animals and the ability of biofilm formation by these bacteria has further aided their survival in unfavorable environment. Characterization of these biofilm producing bacteria isolated from pigs and chicken may lead to formulation of strategies for prevention and control of Salmonella infections. Therefore, the present study was conducted to isolate Salmonella from pigs and poultry of Mizoram, determine their biofilm producing ability by phenotypic and genotypic methods along with their virulence and antimicrobial resistance properties. A total of 15 Salmonella spp. (pig=9, poultry=6) was isolated from 100 faecal samples from pigs and 50 cloacal swabs from poultry and biofilm producing ability of the isolates was determined by microtiter plate assay. A total of 10 (66.67%) isolates were found to be biofilm producer. All the biofilm producing bacterial isolates were investigated for antimicrobial sensitivity and distribution of selected biofilm associated genes (csgA, csgD and adrA), virulence genes (invA, stn and sefA) and antimicrobial resistance (AMR) genes (blaTEM, blaSHV and blaCTX-M). The most prevalent resistance was found against ceftazidime (80%), ceftriaxone (80%), cefixime (70%), cefotaxime (70%), gentamicin (70%), cotrimoxazole (60%) and ampicillin (60%). A total of 7 (70%) isolates were resistant to at least three different classes of antimicrobial agents and considered as multidrug resistant. All the isolates were positive for adrA (100%) but negative for csgA and csgD genes. The most frequent virulence gene was invA (100%) and stn (100%). Among the AMR genes, blaTEM (60%) was found to be the major AMR determinants. Moreover, a total of 7 Salmonella isolates were positive for at least one of t biofilm associated genes, virulence genes and AMR genes.

scholarly journals Antimicrobial resistance, biofilm synthesis and virulence genes in Salmonella isolated from pigs bred on intensive farms

2018 ◽  
Vol 7 (2) ◽  
Author(s):  
Elena Barilli ◽  
Cristina Bacci ◽  
Zulena Stella Villa ◽  
Giuseppe Merialdi ◽  
Mario D’Incau ◽  
...  
Keyword(s):  
Virulence Genes ◽  
Antimicrobial Agents ◽  
Multidrug Resistant ◽  
Surveillance Program ◽  
Esbl Producer ◽  
The North ◽  
Human Salmonellosis ◽  
The Usa ◽  
Foodborne Infection ◽  
Highly Virulent

Salmonella is the second cause of foodborne infection in humans in the USA and Europe. Pigs represent the second most important reservoir for the pathogen and the consumption of pork meat is a major risk factor for human salmonellosis. Here, we evaluated the virulence patterns of eleven Salmonella isolated from pigs (carcasses and faces) bred in intensive farms in the north of Italy. The two serotypes identified were S. Typhimurium and its monophasic variant 1,4,5,12:i:-. None of the isolates was an ESBL producer, as confirmed also by PCR. However, the presence of a multidrug resistant pattern was evident, with all the isolates being resistant to at least to five antimicrobial agents belonging to various classes. Moreover, six out of eleven isolates showed important resistance profiles, such as resistance against colistin and ciprofloxacin, with nine to twelve recorded resistances. The isolates were negative for the biofilm synthesis test, while four different virulotypes were characterized. All the isolates showed the presence of invA, hilA, stn, ssrA, sipC. One sample also harbored ssaR and spvC genes. One strain was positive for all the virulence genes tested and was resistant to 12 antimicrobial agents. The present study contributes new data to the surveillance program for antibiotic resistance. Furthermore, the presence of eleven highly virulent isolates poses concern for human health in relation to their diffusion in the environment.

scholarly journals Genotypic Characterization of Multidrug-Resistant Pseudomonas aeruginosa Using Whole-Genome Sequencing

2021 ◽  
Vol 10 (32) ◽  
Author(s):  
Baha Abdalhamid ◽  
Itidal Reslane ◽  
Emily Mccutchen ◽  
Peter C. Iwen
Keyword(s):  
Health Care ◽  
Pseudomonas Aeruginosa ◽  
Draft Genome ◽  
Multidrug Resistant ◽  
Whole Genome ◽  
Significant Morbidity ◽  
Genome Sequences ◽  
Content Type ◽  
Human Infections

Multidrug-resistant Pseudomonas aeruginosa is a serious threat worldwide causing health care-acquired infections and is associated with significant morbidity and mortality. This report describes the draft genome sequences of five multidrug-resistant Pseudomonas aeruginosa strains isolated from human infections.

scholarly journals Characterization of auto-agglutinating and non-typeable uropathogenic Escherichia coli strains

2019 ◽  
Vol 13 (06) ◽  
pp. 465-472
Author(s):  
Ulises Hernández-Chiñas ◽  
Alejandro Pérez-Ramos ◽  
Laura Belmont-Monroy ◽  
María E Chávez-Berrocal ◽  
Edgar González-Villalobos ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Virulence Genes ◽  
Antimicrobial Agents ◽  
Multidrug Resistant ◽  
Uropathogenic Escherichia Coli ◽  
E Coli ◽  
Resistant Strains ◽  
Tract Infections ◽  
Disk Method

Introduction: Uropathogenic Escherichia coli (UPEC) are the main etiological agent of urinary tract infections (UTIs). Association between different serotypes and UTIs is known, however, some strains are incapable to be serotyped. The aim of this work was to study bthe phenotypical and genotypical characteristics of 113 non-typeable (NT) and auto-agglutinating (AA) E. coli strains, isolated from UTIs in children and adults. Methodology: The 113 UPEC strains were analyzed by PCR assays using specific primers to determine their serogroups, fimH, papC, iutA, sat, hlyCA and cnf1, virulence associated genes, and chuA, yjaA and TSPE4.C2 for phylogroup determination. Additionally, the diffusion disk method was performed to evaluate the antimicrobial resistance to 18 antimicrobial agents. Results: Using the PCR assay, 63% (71) of the strains were genotyped showing O25 and O75 as the most common serogroups. The virulence genes fimH (86%) and iutA (74%) were the most prevalent, in relation to the phylogroups the commensal (A and B1) and virulent (B2 and D) showed similar frequencies (P > 0.05). The antimicrobial susceptibility test showed a high percentage (73%) of multidrug-resistant strains. Conclusions: The genotyping allowed identifying the serogroup in many of the strains that could not be typed by traditional serology. The strains carried virulence genes and were multidrug-resistant in both, commensal and virulent phylogroups. Our findings revealed that, in addition to the classical UPEC serogroups, there are pathogenic serogroups not reported yet.

scholarly journals Comparative Genomics of Multidrug-Resistant Enterococcus spp. isolated from Wastewater Treatment Plants

2019 ◽  
Author(s):  
Haley Sanderson ◽  
Rodrigo Ortega-Polo ◽  
Rahat Zaheer ◽  
Noriko Goji ◽  
Kingsley K. Amoako ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Virulence Genes ◽  
Wastewater Treatment Plants ◽  
Multidrug Resistant ◽  
Multi Drug Resistance ◽  
Conventional Activated Sludge ◽  
Enterococcus Spp ◽  
Aerated Filter ◽  
The Impact

Abstract Background Wastewater treatment plants (WWTPs) are considered hotspots for the environmental dissemination of antimicrobial resistance (AMR) determinants. Vancomycin-Resistant Enterococcus (VRE) are candidates for gauging the degree of AMR bacteria in wastewater. E. faecalis and E. faecium are recognized indicators of fecal contamination in water. Genome comparisons of enterococci isolated from a conventional activated sludge (CAS) and biological aerated filter (BAF) WWTPs were conducted. Results VRE isolates, including E. faecalis (n=24), E. faecium (n=11), E. casseliflavus (n=2) and E. gallinarum (n=2), were selected for sequencing based on source, species and AMR phenotype. The pangenomes of E. faecium and E. faecalis were both open. The genomic fraction related to mobilome had a positive correlation with genome size in E. faecium (p < 0.001) and E. faecalis (p < 0.001) and with the number of AMR genes in E. faecium (p = 0.005). The AMR phenotype generally aligned with genotype. Genes conferring vancomycin resistance, including vanA and vanM (E. faecium), vanG (E. faecalis), and vanC (E. casseliflavus/E. gallinarum), were detected in 20 genomes. The most prominent groups of functional AMR genes were efflux pumps and transporters. A minimum of 16, 6, 5 and 3 virulence genes were detected in E. faecium, E. faecalis, E. casseliflavus and E. gallinarum genomes, respectively. Virulence genes were more common in E. faecalis and E. faecium, than E. casseliflavus and E. gallinarum. A number of mobile genetic elements were shared among species. Functional CRISPR/Cas arrays were detected in 13 E. faecalis genomes, with all but one also containing a prophage. The lack of a functional CRISPR/Cas arrays was associated with multi-drug resistance in E. faecium. Phylogenetic analysis demonstrated differential clustering of isolates based on source but not based on WWTP. Genes related to phage and CRISPR/Cas arrays could potentially serve as environmental biomarkers. Conclusions There was no discernable difference between enterococcal genomes from the CAS and BAF WWTPs either before or after treatment. Understanding the impact of WWTPs on the dissemination of AMR in the environment will require knowledge of the mobility and upregulation of genes and the characterization of mobilomes within WWTPs and surrounding environments.

scholarly journals Analysis of medical prescriptions dispensed at health centers in Belo Horizonte, Minas Gerais, Brazil

2004 ◽  
Vol 20 (1) ◽  
pp. 72-79 ◽  
Author(s):  
Francisco A. Acurcio ◽  
Edson Perini ◽  
Sérgia M. S. Magalhães ◽  
Leonardo G. Vieira Terceiro ◽  
José Maurício Vieira Filho ◽  
...  
Keyword(s):  
Continuing Education ◽  
Health Department ◽  
Minas Gerais ◽  
Health Centers ◽  
Improve Quality ◽  
Minas Gerais State ◽  
Belo Horizonte ◽  
Instructions For Use ◽  
Municipal Health

This article focuses on medical prescriptions dispensed at health centers under the Municipal Health Department in Belo Horizonte, Minas Gerais State, Brazil. The study analyzed 4,607 prescriptions from March to April 1999, grouped according to origin (internal or external). The analysis focused on information written on medical prescriptions. The main findings were: (a) an average of 2.4 drugs per prescription in both groups; (b) prescriptions filled out with 4 or more drugs accounted for 18.0% of internal and 17.6% of external prescriptions; (c) 84.3% of internal and 85.5% of external prescriptions provided no instructions for use of medication; (d) information on dosage regimen varied from 51.2% to 97.6% for internal and 57.9% to 96.5% for external prescriptions; (e) generic names were specified for 51.9% and 28.4% of all drugs on internal and external prescriptions, respectively; (f) prescriptions containing standard drugs from the Municipal Health Department accounted for 88.7% of internal and 76.4% of external prescriptions. Data analysis shows the need for continuing education of physicians and adoption of other methods to improve quality of prescriptions and promote rational use of drugs.

scholarly journals Occurrence and antimicrobial susceptibility of Salmonella isolates recovered from the pig slaughter process in Romania

2015 ◽  
Vol 9 (01) ◽  
pp. 099-104 ◽  
Author(s):  
Adriana Morar ◽  
Claudia Sala ◽  
Kálmán Imre
Keyword(s):  
Antibiotic Resistance ◽  
Antibiotic Susceptibility ◽  
Multidrug Resistant ◽  
Resistance Pattern ◽  
Pork Products ◽  
Human Salmonellosis ◽  
Production Complex ◽  
Amoxicillin Clavulanic Acid ◽  
Meat Samples ◽  
Human Infections

Introduction: Reported human salmonellosis cases have increased in Romania. Antibiotic susceptibility testing of Salmonella strains isolated from pork and chicken meat indicate a worrying multidrug resistance pattern. This study aimed to investigate the occurrence of Salmonella and to evaluate the antibiotic resistance of Salmonella strains in a pig slaughterhouse-processing complex, which receives animals from 30% of the large industrialized swine farms in Romania. Methodology: A total of 108 samples, including pork (n = 47), packaged pork products (n = 44), scald water sludge (n = 8), and detritus from the hair removal machine of the slaughterhouse (n = 9) were examined for the presence of Salmonella through standard methods. The antibiotic susceptibility of the isolated strains to 17 antibiotics was tested using the Vitek 2 system. Results: Twenty-six (24.1%) samples were found to be Salmonella positive; this included 25.5% of meat samples and 15.9% of packaged products, as well as samples from two different points of the slaughter (41.2%). Resistance was observed against tetracycline (61.5%), ampicillin (50%), piperacillin (50%), trimethoprim-sulfamethoxazole (34.6%), amoxicillin/clavulanic acid (26.9%), nitrofurantion (23.1%), cefazolin (15.4%), piperacillin/tazobactam (7.7%), imipenem (3.8%), ciprofloxacin (3.8%), and norfloxacin (3.8%). No resistance towards cefoxitin, cefotaxime, ceftazidime, cefepime, amikacin, and gentamicin was found. Conclusions: Our study demonstrated the occurrence of multidrug-resistant Salmonella strains in the investigated pork production complex and highlighted it as a potential source of human infections. The results demonstrate the seriousness of antibiotic resistance of Salmonella in Romania, while providing a useful insight for the treatment of human salmonellosis by specialists.

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