Serum interleukin-17 and estradiol levels in postmenopausal women in relation to osteoporosis

Background In post-menopausal women, estrogen deficiency leads to instability between bone formation and resorption which is regulated by osteoclastogenic cytokines leading to resorption. Interleukin-17 (IL-17) a proinflammatory cytokine has been found as an important regulator of osteoclast-genesis induced by estrogen deficiency in favor of bone loss in animal studies. The study aimed to evaluate levels of IL-17 and estrogen (E2) in relation to bone mineral density (BMD) and risk of fracture in postmenopausal women with and without osteoporosis. Results IL-17 levels were significantly higher and E2 levels were significantly lower in the osteoporotic group compared to the non-osteoporotic group (P value ≤ 0.01). There was a highly significant difference in DEXA score and FRAX index between two groups: with higher values of FRAX and lower values of DEXA score among osteoporotic group (P value ≤ 0.01). IL-17 was inversely correlated to estrogen level and highly significant negative correlation with DEXA as well as a highly significant positive one with FRAX index. IL-17 serum level was able to diagnose osteoporosis at a cutoff level of > 80 pg/mL with 100% sensitivity, 100% specificity, 100% positive predictive value (PPV), and 100% negative predictive value (NPV). Conclusions Serum IL-17 was significantly elevated in osteoporotic postmenopausal women when compared to healthy postmenopausal ones and was inversely correlated with estrogen level and DEXA.

Astragalus Polysaccharide Injection Ameliorates the Apoptosis of Chondrocytes in the Ovariectomized Osteoporosis Mouse Model via Modulating Protein Kinase B/Glycogen Synthase Kinase 3 Beta Signal Transduction

The Akt/GSK3 signal pathway exerts an impact on the apoptosis of chondrocytes. We planned to elucidate the role and mechanism of Akt/GSK3 signal transduction in ovariectomized osteoporosis. 50 female mice in SPF-graded and healthy condition were processed to establish the ovariectomized osteoporosis model, while 12 of the healthy mice were set as blank controls. The successfully established ovariectomized osteoporosis models were divided into model group (12 mice) and Astragalus group (12 mice). The following measurements were conducted: the bone mineral density in the left humerus, serum estrogen level and Cx43 protein level in osteoblasts, proportion of apoptotic cells, as well as the protein and mRNA levels of Akt and GSK3β in murine bone tissues via Western blotting and RT-PCR detection, respectively. The bone mineral density of mice in Astragalus group was the highest (0.174±0.04) g/cm2. The positive rate of Cx43 protein expression, apoptosis rate, as well as the protein and mRNA levels of Akt in osteocytes were significantly decreased (P < 0.05), but still significantly higher than model group. Estrogen level in Astragalus group was (87.52 ±8.69) pmol/L. The positive rate of Cx43 expression, apoptosis rate, as well as the protein and mRNA levels of GSK3β in osteocytes were decreased in comparison to model group (P < 0.05). Astragalus polysaccharide injection could ameliorate the apoptosis of chondrocytes and downregulate Cx43 protein via modulating Akt/GSK3β signal transduction, thereby exerting a therapeutic effect on ovariectomized osteoporosis.

OMEGA 3 SUPPLEMENTATION DECREASE A BLOOD CHOLESTROL LEVEL IN TRAINED WOMEN

Background : Trained woean who experiencing menstrual disoders are found with high blood cholesterol level. This situation is contrary to who don’t experience menstrual disorder. Mentrual disorder condition has a low estrogen level. The low estrogen level is associated with endothelial disfunction which causes blood cholesterol increase. The benefit of omega 3 supplementation have been clinically proven to prevent atherosclerosis, decrease blood cholesterol levels, and playong an important role in modulating inflamation. Omega 3 supplementation is expected to decrease blood cholesterol levels in trained women. Method: This research is using field experimental with case control method. The samples are 30 trained womens contained by 15 are controlled and 15 are given treatment. The sampling technique is randomized allocation. The independent variabel is omega 3 300mg supplementation and the dependent variabel is blood cholesterol levels. Data are analized using parametric statistic paired T-test. Result: The bivariate analysis showed there is a meaningful difference on blood cholesterol levels in the treatment group p=0,0027 and no change in control group p=0,191 during treatment for 8 weeks. Conclusion: Omega 3 supplementation decrease a blood cholesterol levels in trained women.

Gestational Monosodium Glutamate Exposure Effects on Anogenital Distance of Male Rat Pups

High-dose Monosodium Glutamate (MSG) expo sure increases the estrogen level in pregnant rats. However, there are limited data available on whether the MSG-related maternal hormonal effects can affect male litters' genitalia phenotype. This study aimed to analyze the impact of MSG on estrogen level in pregnant rats and anogenital distance in male pups. Experiment for this study was performed at the animal facility of Biomedical Laboratory at the Faculty of Medicine, Universitas Andalas, from April 2019 to February 2020. Pregnant Wistar rats were given MSG orally at 2 and 4 mg/g body weight (BW) for 20 days. On day 21, pregnant rats were sacrificed and blood was drawn intracardially. Estradiol serum level was measured by ELISA. Male pups were counted, and the anogenital distance (AGD) was measured. Maternal serum estradiol levels were statistically analyzed by One-Way ANOVA and the AGD of male litters were analyzed by the Kruskal-Wallis test. Results showed that perinatal MSG exposure increased the estradiol level (26.3±4.5 pg/ml; 37.5±6.7 pg/ml; 62.1±8.2 pg/ml in control, 2 mg/g BW, and 4 mg/g BW group, respectively [mean±SD; p=<0.001]) and decreased the AGD (4 mm; 3 mm; 1.5 mm in control, 2 mg/g BW, and 4 mg/gBW group, respectively [median; p=<0.01]) in a dose-dependent manner. Thus, MSG exposure during pregnancy is a risk factor for male rat feminization.

Serum level of estrogen in Iranian patients with oral lichen planus

Background Oral lichen planus (OLP) is one of the most common diseases in the oral cavity and has a chronic inflammatory nature. The etiology of this disease remains unclear. OLP is more prevalent in women, but to our knowledge, no study yet evaluated estrogen levels in women with OLP. Objectives To determine the serum level of estrogen in female patients with OLP. Methods This case–control observational study was conducted in patients who were referred to the Department of Oral and Maxillofacial Medicine at the Tehran University of Medical Sciences. After clinical and histopathological confirmation of OLP diagnosis, and according to exclusion and inclusion criteria, 47 women with OLP and 47 healthy women (10 premenopausal and 37 postmenopausal) were included, as case and control groups, respectively. We obtained blood samples (5 mL) from each participant to measure the serum level of 17β-estradiol. Data were analyzed by Spearman rank correlation coefficients and a two-way analysis of variance with Bonferroni post hoc tests. Results Serum estrogen level in women with OLP was significantly higher than that in healthy women (P = 0.002), and it was also significantly higher in premenopausal women than postmenopausal women (P < 0.001). The severity of OLP lesions correlated with estrogen level in postmenopausal women (r = 0.650; P < 0.001); in premenopausal women (r = 0.618; P = 0.008), and in all women with OLP (r = 0.535; P < 0.001). Conclusion OLP, like other autoimmune diseases, is affected by the serum level of sex hormones such as estrogen.

Time-sensitive effects of Zhuang Medicated Thread Moxibustion on Estrogen Level in female perimenopausal Model Rats.

Background: To ascertain the estrogenic effect of Zhuang Medicated Thread Moxibustion (ZMTM) and explore its time-sensitive impact on estradiol in female perimenopausal rats. Methods: Female rats were randomized into four groups of 10 rats, each consisting of the control, model, ZMTM, and acupuncture groups. The perimenopausal syndrome was induced in the last three groups with a daily subcutaneous dose of 80 mg/kg of 4- vinylcyclohexene diepoxide for 15 days. Afterward, rats in the model and control groups were fed routinely, while rats in the ZMTM and acupuncture groups were treated with six ZMTM and acupuncture courses, respectively. The rats’ general condition and estradiol (E2) levels in the rats’ serum were assessed. Results: Following the six courses of treatment, the E2 level in the model group was significantly the lowest, while the regular group was the highest (P < 0.05). There was also a gradual increase in the E2 level of the ZMTM group compared to the model and acupuncture groups, such that after the 5th and 6th courses of treatment, their E2 level was significantly higher than the model and acupuncture groups (P < 0.05). The control group had a better condition than all other groups, while the ZMTM group was better than the model and acupuncture groups. Conclusion: ZMTM can improve perimenopausal induced rats’ estrogen level, and this effect becomes better with each further treatment course.

Dynamics of proximal and distal transport of sodium in case of bilateral gonadectomy and the efficacy of hormonal replacement therapy

Introduction. The characteristic feature of current trauma in Ukraine and in the world is the prevalence of highly energetic skeletal trauma which is followed by the appearance of combined and multiple injuries with the formation of multiorgan dysfunction and failure. Because of the increase of life duration, the high interest of current traumatology is the investigation process of trauma course in older people. Among them the high interest is given to females in postmenopausal period. The decrease of estrogen level causes very unfavorable ground for the trauma course. However, the influence of severe trauma on changes in proximal and distal transport of sodium in case of hypoestrogenic state is not fully investigated. There is no available data concerning the effectiveness of hormonal replacement therapy in this condition.The goal of the study: is to investigate the influence of cranioskeletal injury on the dynamics of proximal and distal sodium transport in rats with bilateral ovariectomy in the period of late changes of traumatic disease and evaluate the effectiveness of hormonal replacement therapy. Materials and methods. Experiments were performed on 42 non-linear white female rats with the weight of 200-220 g. The model of hypoestrogenic state was performed via surgical removal of the ovaries. 1 month after the gonadectomy in rats skeletal trauma was performed. As a treatment option in one subgroup of rats with gonadectomy and cranioskeletal injury we performed hormonal replacement therapy. The control groups were formed by intact rats and osteoporotic rats 1 month after gonadectomy which were not injured. In control groups and after 1 and 2 months of posttraumatic period we were investigating the functional state of kidneys by performing the water upload method. Afterwards we performed the laboratory checkup of creatinine and sodium levels in serum and urine and additionally calculated the values of proximal and distal sodium transport. Results and its discussion. It was found that the level of proximal and distal sodium transport depends on the estrogen level, the decrease of its secretion leads to the decrease of sodium reabsorption in tubules and the increase in secretion. These changes were more prevalent in proximal tubules. In the course of trauma 1 month after posttraumatic period the value was decreasing in both research groups. However, in rats with gonadectomy the changes were much bigger and stayed at the same level until the 2nd month of the experiment. In rats without gonadectomy these values were achieving the same levels as control group until 2nd month. The use of combined hormonal replacement therapy caused the increase of proximal and distal sodium transport in comparison to the group without receiving treatment. The results that we got though was not achieving the level of rats without gonadectomy, but it showed the perspective for the use of combined hormonal replacement therapy in case of hypoestrogenic state for the decrease of renal dysfunction. Conclusions: Bilateral ovariectomy in female rats after 1 month shows the decrease of proximal and distal sodium levels comparing to rats with preserved gonads. The cranioskeletal injury that happened 1 month after bilateral ovariectomy causes the much bigger decrease of proximal and distal sodium transport comparing to rats with preserved gonads. The use of combined hormonal replacement therapy caused lower changes in proximal and distal sodium transport comparing to rats without this treatment.