scholarly journals Testicular and epididymal histomorphometric assessment in F1 male Wistar rats after gestational Ricinus communis oil exposure

2015 ◽  
Vol 32 (03) ◽  
pp. 187-191 ◽  
Author(s):  
S. Salami ◽  
J. Omirinde ◽  
M. Balogun ◽  
Y. Raji
Keyword(s):  
Wistar Rats ◽  
Ricinus Communis ◽  
Male Rats ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Critical Periods ◽  
Luminal Diameter ◽  
Male Wistar Rats ◽  
And Function ◽  
Tubular Diameter

Abstract Introduction: Fetal programming hypothesis presupposes that stimulus or insult acting during critical periods of uterine growth and development do alter tissue structure and function. In the present study changes in histomorphometric integrity of the testes and epididymis in adult F1 male rats maternally exposed to Ricinus communis oil (RCO) at different gestation periods were assessed. Materials and Methods: Therapeutic dose of RCO 950 mg/kg BW was administered to pregnant Wistar rats at gestation days GD 1-7, 7-14, 14-21 and 1-21 respectively. Testes and epididymis of adult male F1 offspring were then harvested for Histomorphometry assessment under Light microscope. Seminiferous tubular diameter (STD), seminiferous luminal diameter (SLD) and Seminiferous epithelial height (EH) of both peripheral and central seminiferous tubules were measured in the testes. Epididymal tubular diameter (ETD), epididymal luminal diameter (ELD) and epididymal epithelial height (EEH) were measured in epididymis. Results: Results were expressed as the mean ± SEM and significance taken at p<0.05. STD significantly (p<0.001) increased in F1 males from GD1-7, 7-14, 14-21 relative to control group. SEH significantly (p<0.001) decreased in F1 males from GD1-7,7-14 and 1-21. SLD increased significantly (p<0.001) in F1males from GD1-7, 7-14, 14-21 and 1-21. ETD significantly (p<0.01) increased in F1 males from GD1-7, 7-14, 14-21. EEH significantly (p<0.001) increased in F1 males from GD1-7 and GD7-14. ELD however increased significantly (p<0.001) only in F1 males from GD1-7. Conclusion: Maternal RCO exposure at different gestation periods impaired negatively histomorphometry of the testis and epididymis in male offspring.

scholarly journals Histomorphological Effects of Cottonseed Oil on Testes in Adult Male Wistar Rats

2018 ◽  
Vol 25 (4) ◽  
Author(s):  
Lilian Ebele Chris-Ozoko ◽  
Onome Okpe ◽  
Wilson Josiah Iju ◽  
John Chukwuma Oyem
Keyword(s):  
Adult Male ◽  
Wistar Rats ◽  
Cottonseed Oil ◽  
Male Rats ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Luminal Diameter ◽  
Orogastric Tube ◽  
Group I ◽  
Male Wistar Rats

Cottonseed oil is an extract from the seeds of cotton plant, that is rich in protein, carbohydrate and fiber.          The objective of the research was to study the effect of cottonseed oil on the histology of the testis.          Materials and methods. Twelve adult male Wistar rats weighing 200 – 250g, were randomly sorted into four groups (I - IV), each containing three animals. Cottonseed oil was administered orally to the experimental animals by means of an orogastric tube. The rats in Group I (the control group) received standard rat pellet and oral normal saline ad libitum only. The animals in Groups II, III, and IV received 0.2 ml, 0.6 ml and 1.0 ml of cotton seed oil per body weight respectively. On the 21st day, the animals were sacrificed by cervical dislocation. The testes were excised and, then, fixed with appropriate fixatives for routine tissue processing.          Results. The results revealed an increase in the luminal diameter of seminiferous tubules, a decrease in the Leydig cell population, the disorientation of spermatogenic series and their detachment from the germinal epithelium.          Conclusions. The present study demonstrated that oral administration of cottonseed oil to male rats displayed a deleterious effect on their testis by disrupting spermatogenesis and the histological architecture of the testis.

scholarly journals Influence of prolonged flaxseed ( Linum usitatissimum ) consumption over epididymis and testicle histoarchitecture of Wistar rats

2017 ◽  
Vol 37 (6) ◽  
pp. 650-656 ◽  
Author(s):  
Lanna B.N.S. Corrêa ◽  
Ludmila F.M. de F. Cardozo ◽  
Ilma C. de A. Ribeiro ◽  
Gilson T. Boaventura ◽  
Maurício A. Chagas
Keyword(s):  
Sertoli Cell ◽  
Wistar Rats ◽  
Serum Levels ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Lactation Period ◽  
Luminal Diameter ◽  
Flaxseed Meal ◽  
Male Wistar Rats ◽  
Hormonal Evaluation

ABSTRACT: Flaxseed is considered a functional food with several health benefits. However, because of its high phytoestrogen content, flaxseed influences hormone metabolism and affects the gonadal biomorphology. In this study, computerized histomorphometry was used to evaluate seminiferous and epididymal tubules, considering the different regions of the epididymis (head, body and tail) of rats subjected to a prolonged diet of flaxseed. Young adult male Wistar rats (n=20) were divided into 2 groups during their lactation period: Control Group (CG), fed casein-based meals and Flaxseed Group (FG), fed a 25% flaxseed meal. After 250 days of continuous ingestion, the animals were euthanized and a blood sample was collected. The testicles and epididymis were removed and fixed in buffered formalin solution. The samples were subjected to routine histological paraffin techniques and stained with hematoxilin and eosin. Immunostaining was performed using an antivimentin antibody for Sertoli cell identification. For morphometry, images of the slides were scanned and analyzed using Image J to determine the epithelial height, tubular and luminal diameter and tubular and luminal area. In the hormonal evaluation, FG had a higher serum concentration of estrogen (P=0.001), but no change was observed in the concentration of testosterone. The morphometric assay of seminiferous tubules and epididymal regions revealed no significant differences between the analyzed groups. Similarly, Sertoli cell quantification showed no significant differences in the FG (P=0.98). These results revealed that the continuous and prolonged intake of 25% flaxseed meals from gestation to 250 days of age, even with a significant increase in serum levels of estradiol, does not exert adverse effects on the testicular and epididymal structure or on the cells participating in the spermatogenesis of rats.

scholarly journals Azathioprine and Methotrexate impaired the morphology and functions of the testes in adult wistar rats

2014 ◽  
Vol 31 (02) ◽  
pp. 075-081
Author(s):  
A. Akinlolu ◽  
O. Akinola ◽  
P. Khobe ◽  
K. Obasi ◽  
O. Dada
Keyword(s):  
Adult Male ◽  
Wistar Rats ◽  
Follicle Stimulating Hormone ◽  
Physiological Saline ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Connective Tissues ◽  
Group I ◽  
Male Wistar Rats ◽  
Dose Dependent

Abstract Introduction: AAzathioprine and Methotrexate are both used in the treatment of cancer; and are classified as cytotoxic drugs with reported adverse effects such as oxidative damage to the DNA/RNA, the testes and sperm cells. This study, therefore, tested the hypothesis that AAzathioprine and Methotrexate administrations impair the morphology and functions of the testes in adult male wistar rats. Methods: AAzathioprine (50-150mg per day) and Methotrexate (2.5mg per week) are used in the treatment of cancer in adult Man. We tested the hypothesis that AAzathioprine and Methotrexate impair the morphology and functions of testes in rats. Forty adult male wistar rats (150-230g) were employed in the study: Control Group I received physiological saline while Experimental Groups II - V received oral administrations of 5mg/kg/bodyweight of AAzathioprine per day, 15mg/kg/bodyweight of AAzathioprine per day, 8mg/kg/bodyweight of Methotrexate per week and 20mg/kg/bodyweight of Methotrexate per week respectively for 35 days. Results: Histological examinations of the testes of rats of Groups II - V showed dose-dependent morphological anomalies such as fewer collagen ibers of connective tissues, disrupted seminiferous tubules and scanty spermatozoa when compared to rats of Group I. Statistical analyses showed dose-dependent elevated levels (P≤0.05) of superoxide dismutase and malondialdehyde in testes homogenates of rats of Groups II - V when compared to rats of Group I. This implied increased oxidative stress in rats of Groups II - V. Evaluations of Follicle Stimulating Hormone and Testosterone showed dose-dependent significantly elevated levels (P≤0.05) in rats of Groups II - V when compared to rats of Group I. Conclusions: Our findings are consistent with the stated hypothesis.

scholarly journals Upregulation of Kiss-1 and Gpr54 Genes Expression in Pituitary of Male Rats Following the Central Administration of Neuropeptide Y

2019 ◽  
Vol 4 (4) ◽  
pp. 137-142
Author(s):  
Vahid Azizi ◽  
Shahrbanoo Oryan ◽  
Homayuon Khazali ◽  
Abdolkarim Hosseini
Keyword(s):  
Gene Expression ◽  
Pituitary Gland ◽  
Neuropeptide Y ◽  
Wistar Rats ◽  
Third Ventricle ◽  
Male Rats ◽  
Control Group ◽  
Central Administration ◽  
Reproductive Axis ◽  
Male Wistar Rats

Introduction: The neuropeptide Y (NPY) in the neural circuits of the hypothalamus has a stimulating effect on reproductive activities in mammals. Kisspeptin (KiSS1) is a quintessential neurotransmitter in the reproductive axis which directly stimulates gonadotropin-releasing hormone neurons in the hypothalamus. The distribution of KiSS1 expressing cells in the pituitary was described previously. Despite earlier reports showing the KiSS1 receptor, G-protein coupled receptor 54 (GPR54) expression in the pituitary, the potential physiological roles of kisspeptin at this gland have remained obscure. Accordingly, this study investigated the role of NPY on the relative expression of Kiss1 and Gpr54 genes in the pituitary gland in male Wistar rats. Methods: In general, 20 male Wistar rats weighing 200-250 g in 4 groups (5 in each group) received saline, NPY (2.3 nM), BIBP3226 (NPY receptor antagonist, 7.8 nM), and NPY+ BIBP3226. Then, they received the simultaneous injection of these molecules through the third ventricle of the brain. Finally, the relative mean expressions of Kiss1 and Gpr54 genes in the anterior pituitary were quantitatively analyzed by the real-time polymerase chain reaction. Results: The central injection of NPY increased the relative mean expressions of Kiss1 and Gpr54 genes in the pituitary gland compared to the control group although the injection of BIBP3226 eradicated these effects. However, the gene expression of Gpr54 in the rats receiving NPY coupled with BIBP3226 in hypophysis in comparison to the group receiving only NPY demonstrated a significant reduction (P<0.05). Conclusion: Overall, the central injection of NPY stimulated the gene expression of Kiss1 and Gpr54 in the pituitary gland.

scholarly journals The Effect of Soy Milk on Mounting Latency, Mounting Frequency, and Reproductive Development in Male Wistar Rats (Rattus Norvegicus)

2021 ◽  
Vol 9 (B) ◽  
pp. 670-678
Author(s):  
Nurdiana Nurdiana ◽  
Pradnyawati Chania ◽  
Rifzi Nurvitasari ◽  
Azmiatun Nisa ◽  
Styan Wahyu Diana ◽  
...  
Keyword(s):  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Reproductive Development ◽  
Normal Diet ◽  
Male Rats ◽  
Male Rat ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Soy Milk ◽  
Male Wistar Rats

AIM: This research aims to examine the effects of soy milk on mounting latency (ML), mounting frequency (MF), estrogen levels, androgen-binding protein (ABP) expression, and spermatogenesis in male rats (Rattus norvegicus). METHODS: Twenty-four male wistar rats (Rattus norvegicus) aged 4 weeks were divided into four groups. Control group (given a normal diet), P1; P2; P3 (given the normal diet and soy milk powder at doses of 7.1; 14.2; 21.3 g/KgBW/day, respectively) for 6 weeks. Observation of ML and MF were performed at 9 weeks 5 days of age, and rat surgery was performed at 10 weeks of age. Analysis of estrogen hormone levels was conducted by enzyme-linked immunosorbent assay (ELISA), ABP staining was using immunohistochemistry method, testicular spermatogenesis was observed using histopathological methods, and observation of spermatozoa was performed under the microscope.  RESULTS: The results showed no significant reduction of ML and MF, estrogen levels, and ABP expression (p ≤ 0.256; 0.865; 0.959, respectively) in male rat, but there was a significant decrease in the number, morphology, motility of spermatozoa, and testicular histophatology, (p ≤ 0.000, 0.003, 0.008, 0.000, respectively). CONCLUSION: The administrassion of soy milk in various doses (7.1;14.2;21.3 g/KgBW/day) in male Wistar rats (Rattus norvegicus) had showed significantly difference on histopathological evaluation using Johnson’s scoring system, sperm quantity and quality, while on mounting latency and frequency, estrogen levels, and ABP expressions did not show significantly difference between groups. That describe of isoflavone in soy milk can affect several aspects related to male endocrine and reproductive development.

scholarly journals THE EFFECTS OF NICOTINE EXPOSURE PER INHALATION TO THE CHANGE OF MOTILITY AND MORPHOLOGY OF THE RAT’S SPERM

2019 ◽  
Vol 26 (2) ◽  
Author(s):  
Puruhito Eko Nugroho ◽  
Fikri Rizaldi ◽  
Wahjoe Djati Soesanto
Keyword(s):  
Wistar Rats ◽  
Male Rats ◽  
Control Group ◽  
Nicotine Exposure ◽  
Motile Sperm ◽  
Normal Morphology ◽  
Sperm Analysis ◽  
Treatment Groups ◽  
Male Wistar Rats ◽  
The Difference

Objective: Smoking are known to have detrimental effect on sperm. The effects are suspiciously come from nicotine as its main pharmacologic component. This study is carried out to analyze the effect of nicotine exposure per inhalation to the difference of motile sperm percentage and normal morphology sperm percentage of male wistar rats. Material & Methods: Thirty male rats were used for this research. They were randomly divided into 5 groups and treated with nicotine inhalation 0.5 mg/kgBW,  1 mg/kgBW, 2 mg/kgBW and 4 mg/kgBW for 30 days while the control rats were not given any treatment. At the end of experiment, sperm analysis was carried out to measure the percentage of motile sperm and percentage of sperm with normal morphology. Results: Percentage of motile sperm significantly decreased (p<0.05) in all treatment groups compared to control group. Comparison between treatment groups revealed that the percentage of motile sperm significantly decreased in 0.5 mg/kgBW vs 2 mg/kgBW, 0.5 mg/kgBW vs 4 mg/kgBW, and 1 mg/kgBW vs 4 mg/kgBW. Percentage of normal morphology sperm decreased significantly (p<0.05) in 1 mg/kgBW, 2 mg/kgBW, and 4 mg/kgBW compared to control groups. Comparison between treatment groups revealed that there were no significant decreased between each treatment groups. Conclusion: This research conclude that nicotine exposure per inhalation can deteriorate the percentage of motile sperm and the percentage of normal morphology sperm of male wistar rats.

scholarly journals Anti-fertility effect of hydro-alcoholic extract of fennel (Foeniculum vulgare Mill) seed in male Wistar rats

2016 ◽  
Vol 60 (3) ◽  
pp. 357-363 ◽  
Author(s):  
Esrafil Mansouri ◽  
Majid Asadi-Samani ◽  
Wesam Kooti ◽  
Maryam Ghasemiboroon ◽  
Damoon Ashtary-Larky ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Sperm Count ◽  
Seed Extract ◽  
Male Rats ◽  
Control Group ◽  
Alcoholic Extract ◽  
Foeniculum Vulgare ◽  
Fertility Effect ◽  
Male Wistar Rats ◽  
The Right

AbstractIntroduction: The study aimed to investigate the anti-fertility effect of fennel (Foeniculim vulgare Mill) seed extract in male rats.Material and Methods: Forty Wistar rats were divided into five equal groups. The control group received distilled water and the experimental groups were orally administered 1 ml of hydro-alcoholic extract of fennel seed in four doses of 35, 70, 140, and 280 mg/kg/b.w. daily for 60 days. After the last gavage, the rats were anaesthetised and the caudal part of the right epididymis was used for sperm counting. After fixation of the testes, microscopic sections were prepared and histological changes were evaluated.Results: The number of spermatogonia after doses of 140 and 280 mg/kg and Sertoli cells after a dose of 140 mg/kg decreased significantly as compared with the control group (P < 0.05). The number of primary spermatocytes and sperm count decreased significantly in the experimental groups (70, 140, and 280 mg/kg) when compared to the control group (P < 0.05). Furthermore, thickening of the basement membrane, cell apoptosis, and irregular arrangement of the germinal epithelium were observed in the experimental groups.Conclusion: Hydro-alcoholic fennel seed extract at these doses could reduce reproductivity and has anti-fertility activity in male rats.

scholarly journals Gestational Exposure to Synthetic Steroid Hormones Impaired Sperm Quantity and Quality in Wistar Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Samy Ismail Ahmed ◽  
Aamir Magzoub ◽  
Mohammed Saeed Zayed Al-Ayed ◽  
Gamal Ali Attia ◽  
Basel A. Abdel-Wahab ◽  
...  
Keyword(s):  
Prenatal Exposure ◽  
Wistar Rats ◽  
Sperm Count ◽  
Test Group ◽  
Serum Testosterone ◽  
Reproductive Hormones ◽  
Control Group ◽  
Gestational Exposure ◽  
Male Wistar Rats ◽  
And Function

This study was designed to investigate the effect of prenatal exposure to synthetic sex steroid on sperm quantity and quality, relative testicular and epididymal weights, and reproductive hormones level in adult Wistar rats. Forty male Wistar rats were divided into two groups: a test group (n = 20) that included mature rats that were born to dams exposed to gestational treatment with hydroxyprogesterone and a control group (n = 20) that included mature rats born to untreated dams. Compared to the control group, the test group showed a significant reduction in the sperm count, viability and motility, relative testicular and epididymal weights together with increased abnormal spermatozoa (p<0.001). The reproductive hormonal assay revealed significantly lower serum testosterone and higher levels of FSH and LH among the test groups compared to the control (p<0.05 for all). Prenatal exposure to synthetic progesterone negatively affected sperm production and function, relative testicular and epididymal weights, and reproductive hormone levels.

scholarly journals Evaluating the Effects of Noise Pollution on the Levels of Blood Cortisol, Testosterone, and Thyroid in Male Wistar Rats

Health Scope ◽  
2021 ◽  
Vol 10 (3) ◽  
Author(s):  
Seyed-Hosein Abtahi-Eivary ◽  
Ali Tajpoor ◽  
Ali Firoozi ◽  
Shahrzad Mehrzad ◽  
Mohammad Hosein Beheshti
Keyword(s):  
Wistar Rats ◽  
Statistical Tests ◽  
Noise Pollution ◽  
Well Being ◽  
Serum Levels ◽  
Sound Level ◽  
Male Rats ◽  
Control Group ◽  
Male Wistar Rats ◽  
Commercial Kits

Background: Noise pollution is a global problem causing changes in the secretion of various hormones and consequently affecting social well-being and quality of life in cities. Objectives: This study aimed to investigate the effect of noise pollution on the levels of testosterone, thyroid, and cortisol hormones in male rats. Methods: In this experimental study, a total of 70 male Wistar rats (200 - 250 g) were randomly assigned into one control and six experimental groups, with 10 rats in each group. Experimental groups were exposed to noise with different intensity (dB) and time (min) as follows: (I) 60 dB, 30 min; (II) 60 dB, 60 min; (III) 85 dB, 30 min; (IV) 85 dB, 60 min; (V) 110 dB, 30 min; (VI) 110 dB, 60 min; (VII) controls. Animals in the experimental groups were exposed to noise in an acoustic chamber designed for this purpose for 50 days. The Noise.exe software was used to generate noise, and the sound level meter (model TES 1358) was used to determine the accuracy of the intensity and frequency of sound. To determine plasma levels of the hormones, appropriate research and commercial kits were used, which were based on the ELISA method. To determine the concentration of hormones other than TSH, human assay kits were used. All statistical tests were performed in SPSS software version 21. Results: Serum levels of cortisol in the 110-dB (30 and 60 min), 65-dB (60 min), and 85-dB (60 min) groups were significantly higher than the control group (P ≤ 0.05). Also, cortisol levels in the 65-dB and 85-dB (30 minutes) groups were higher than the control group; however, the increase was not significant (P > 0.05). The levels of T4, T3, and TSH in the 60-dB and 85-dB groups were significantly lower than in the control group (P ≤ 0.05). The serum levels of T4, T3, and TSH hormones in the 110-dB group were insignificantly lower than the control group (P > 0.05). The serum level of testosterone in the 110-dB group was significantly lower than the control group (P ≤ 0.05). The mean serum levels of testosterone in the 65-dB and 85-dB groups were insignificantly lower than the control group (P > 0.05). Conclusions: Based on this study, exposure to noise pollution increased cortisol secretion and decreased T4, T3, TSH, and testosterone levels in rats. As this hormonal imbalance may create direct and indirect effects, studies and strategies are recommended to control the imbalance of hormones in the polluted environments.

scholarly journals Testicular cytoprotective activities of Curcuma longa in STZ-induced diabetic adult male wistar rats

1970 ◽  
Vol 6 (1) ◽  
pp. 905-913
Author(s):  
A.J Olanrewaju ◽  
J.O. Owolabi ◽  
S.Y. Olatunji ◽  
A.B.O. Desalu ◽  
W.C. Amaechi
Keyword(s):  
Oxidative Stress ◽  
Leydig Cells ◽  
Wistar Rats ◽  
Curcuma Longa ◽  
Chow Group ◽  
Reproductive Age ◽  
Male Rats ◽  
Seminiferous Tubules ◽  
Histological Structure ◽  
Male Wistar Rats

This study was aimed at investigating the cytoprotective activities of Curcuma longa (Turmeric) on the histological structure of the testes in diabetic male rats. Turmeric is commonly called the golden spice, is used as a spice in cooking and also has a long history of medicinal use, dating back nearly 4000 years to the Vedic culture in India. Its major component is the curcumin that is found to be a natural antioxidant. Diabetes affects large number of young men of reproductive age. It is among a number of disorders associated with oxidative stress. There has been a relationship established between the reduction in glucose load and insulin resistance reduction and oxidative stress in people. Male wistar rats (n=24) were allocated randomly into six groups, 4 in each. Group 1: Control were given only water and standard rat chow; Group 2 and 3: turmeric (100mg/kg) and (25mg/kg) respectively; Group 4: induced with 30mg/kg of streptozotocin (STZ) for 3 days; Group 5: STZ with turmeric (100mg/kg); Group 6: STZ with turmeric (25mg/kg). At the end of the experiment (21 days), the testes were excised for histological analysis. The seminiferous tubules, seminiferous epithelium, Leydig cells were shown to be enhanced in the histoarchitecture of the testes of the treated diabetic groups and did not show any toxicity on the testes in the non-diabetic groups. In conclusion, this study has shown that turmeric does not only possess a non-toxic effect but has cytoprotective effects on the histoarchitecture of the testes in diabetic rats.Keywords: histo-architecture, leydig cells, diabetes, oxidative stress, testes, curcumin.

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