scholarly journals Gestational Exposure to Synthetic Steroid Hormones Impaired Sperm Quantity and Quality in Wistar Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Samy Ismail Ahmed ◽  
Aamir Magzoub ◽  
Mohammed Saeed Zayed Al-Ayed ◽  
Gamal Ali Attia ◽  
Basel A. Abdel-Wahab ◽  
...  
Keyword(s):  
Prenatal Exposure ◽  
Wistar Rats ◽  
Sperm Count ◽  
Test Group ◽  
Serum Testosterone ◽  
Reproductive Hormones ◽  
Control Group ◽  
Gestational Exposure ◽  
Male Wistar Rats ◽  
And Function

This study was designed to investigate the effect of prenatal exposure to synthetic sex steroid on sperm quantity and quality, relative testicular and epididymal weights, and reproductive hormones level in adult Wistar rats. Forty male Wistar rats were divided into two groups: a test group (n = 20) that included mature rats that were born to dams exposed to gestational treatment with hydroxyprogesterone and a control group (n = 20) that included mature rats born to untreated dams. Compared to the control group, the test group showed a significant reduction in the sperm count, viability and motility, relative testicular and epididymal weights together with increased abnormal spermatozoa (p<0.001). The reproductive hormonal assay revealed significantly lower serum testosterone and higher levels of FSH and LH among the test groups compared to the control (p<0.05 for all). Prenatal exposure to synthetic progesterone negatively affected sperm production and function, relative testicular and epididymal weights, and reproductive hormone levels.

Adverse reproductive effects of ethanolic root extract of Waltheria indica in male Wistar rats

2019 ◽  
Vol 16 (4) ◽  
Author(s):  
Afisu Basiru ◽  
Ganiu Jimoh Akorede ◽  
Kehinde Soetan ◽  
Funsho O. Olayemi
Keyword(s):  
Adverse Effect ◽  
Wistar Rats ◽  
Sperm Count ◽  
Ethanolic Extract ◽  
Reproductive Hormones ◽  
Male Reproduction ◽  
Sperm Parameters ◽  
Control Group ◽  
Root Extract ◽  
Male Wistar Rats

Abstract Background Numerous uses of Waltheria indica plant such as antitrypanosomal, antibacterial and antimalarial effects have been reported. It has however been reported that most plants with antibacterial and antiprotozoal effects have adverse effect on male reproduction. Hence, we evaluated the effect of Waltheria indica root on male reproductive parameters. Methods Twenty adult male Wistar rats were randomly divided into four groups (n=5); A–D. Group A served as control group while groups B, C and D were administered with 200, 400 and 800 mg/Kg body weight of crude ethanolic extract of Waltheria indica root. After 28 days of administration, the rats were sacrificed and sperm parameters, sperm morphology, serum reproductive hormones and lipids were determined. Results There was a significant reduction in sperm count and motility as well as significant increase in percentage abnormal sperm cell (p<0.001) at the 400 and 800 mg/kg BW. The serum levels of testosterone was also significantly reduced while total cholesterol increased significantly (p<0.05) at the highest dose. Conclusion Waltheria indica root has adverse effect on male reproduction through reduction in sperm parameters and male reproductive hormones.

scholarly journals Testicular and epididymal histomorphometric assessment in F1 male Wistar rats after gestational Ricinus communis oil exposure

2015 ◽  
Vol 32 (03) ◽  
pp. 187-191 ◽  
Author(s):  
S. Salami ◽  
J. Omirinde ◽  
M. Balogun ◽  
Y. Raji
Keyword(s):  
Wistar Rats ◽  
Ricinus Communis ◽  
Male Rats ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Critical Periods ◽  
Luminal Diameter ◽  
Male Wistar Rats ◽  
And Function ◽  
Tubular Diameter

Abstract Introduction: Fetal programming hypothesis presupposes that stimulus or insult acting during critical periods of uterine growth and development do alter tissue structure and function. In the present study changes in histomorphometric integrity of the testes and epididymis in adult F1 male rats maternally exposed to Ricinus communis oil (RCO) at different gestation periods were assessed. Materials and Methods: Therapeutic dose of RCO 950 mg/kg BW was administered to pregnant Wistar rats at gestation days GD 1-7, 7-14, 14-21 and 1-21 respectively. Testes and epididymis of adult male F1 offspring were then harvested for Histomorphometry assessment under Light microscope. Seminiferous tubular diameter (STD), seminiferous luminal diameter (SLD) and Seminiferous epithelial height (EH) of both peripheral and central seminiferous tubules were measured in the testes. Epididymal tubular diameter (ETD), epididymal luminal diameter (ELD) and epididymal epithelial height (EEH) were measured in epididymis. Results: Results were expressed as the mean ± SEM and significance taken at p<0.05. STD significantly (p<0.001) increased in F1 males from GD1-7, 7-14, 14-21 relative to control group. SEH significantly (p<0.001) decreased in F1 males from GD1-7,7-14 and 1-21. SLD increased significantly (p<0.001) in F1males from GD1-7, 7-14, 14-21 and 1-21. ETD significantly (p<0.01) increased in F1 males from GD1-7, 7-14, 14-21. EEH significantly (p<0.001) increased in F1 males from GD1-7 and GD7-14. ELD however increased significantly (p<0.001) only in F1 males from GD1-7. Conclusion: Maternal RCO exposure at different gestation periods impaired negatively histomorphometry of the testis and epididymis in male offspring.

scholarly journals Anti-fertility effect of hydro-alcoholic extract of fennel (Foeniculum vulgare Mill) seed in male Wistar rats

2016 ◽  
Vol 60 (3) ◽  
pp. 357-363 ◽  
Author(s):  
Esrafil Mansouri ◽  
Majid Asadi-Samani ◽  
Wesam Kooti ◽  
Maryam Ghasemiboroon ◽  
Damoon Ashtary-Larky ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Sperm Count ◽  
Seed Extract ◽  
Male Rats ◽  
Control Group ◽  
Alcoholic Extract ◽  
Foeniculum Vulgare ◽  
Fertility Effect ◽  
Male Wistar Rats ◽  
The Right

AbstractIntroduction: The study aimed to investigate the anti-fertility effect of fennel (Foeniculim vulgare Mill) seed extract in male rats.Material and Methods: Forty Wistar rats were divided into five equal groups. The control group received distilled water and the experimental groups were orally administered 1 ml of hydro-alcoholic extract of fennel seed in four doses of 35, 70, 140, and 280 mg/kg/b.w. daily for 60 days. After the last gavage, the rats were anaesthetised and the caudal part of the right epididymis was used for sperm counting. After fixation of the testes, microscopic sections were prepared and histological changes were evaluated.Results: The number of spermatogonia after doses of 140 and 280 mg/kg and Sertoli cells after a dose of 140 mg/kg decreased significantly as compared with the control group (P < 0.05). The number of primary spermatocytes and sperm count decreased significantly in the experimental groups (70, 140, and 280 mg/kg) when compared to the control group (P < 0.05). Furthermore, thickening of the basement membrane, cell apoptosis, and irregular arrangement of the germinal epithelium were observed in the experimental groups.Conclusion: Hydro-alcoholic fennel seed extract at these doses could reduce reproductivity and has anti-fertility activity in male rats.

scholarly journals Comparative Modulation of the Reproductive System by Ethanolleaf Extracts of Asystasia Gangetica Andanthocleista Vogelli in Male Wistar Rats

2020 ◽  
Vol 2 (3) ◽  
Author(s):  
Simeon I. Egba ◽  
C. O. Okonkwo ◽  
H. C. Omeoga ◽  
I. E. Ekong
Keyword(s):  
Luteinizing Hormone ◽  
Reproductive System ◽  
Wistar Rats ◽  
Follicle Stimulating Hormone ◽  
Reproductive Hormones ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Albino Rats ◽  
Male Wistar Rats ◽  
Stimulating Hormone

A number of medicinal plants have been found to influence the level of reproductive hormones and thus affect fertility in the overall. This work evaluated the effects of ethanol leaf extracts of A. gangetica and A. vogelli respectively on some reproductive system parameters in male Wistar rats. A total of sixteen (16) male albino rats were grouped into four (four rats in each group): group A served as normal control, group B received Immunace (Vitabiotics) group C and D received 400 mg/kg body weight of A. gangetica and A. vogelli extracts respectively. Extracts were administered orally to rats for 21 days, after which they were sacrificed by cervical dislocations and blood samples drawn by cardiac puncture. The effect of the extracts on testosterone, follicle stimulating hormone, luteinizing hormone and semen analysis of the test rats were determined using enzyme linked immunosorbent assay (ELISA) and standard techniques. Data collected were analyzed using Graph pad prism V6 and p values < 0.05 were adopted as significant. There was no significant (p>0.05) change in testosterone, FSH and luteinizing hormones in the group administered A. gangetica plant extract relative to the control. There was also no visible difference in the testes weight and sperm morphology relative to the control group. In contrast, administration of A. vogelli extract caused significant (p<0.05) decrease in testosterone and follicle stimulating hormone concentrations from: 1.12 ± 0.20 to 0.89 ± 0.05 and 1.41 ± 0.07 to 1.35 ± 0.12 respectively relative to the control group. While significant (p<0.05) decrease in luteinizing hormone (1.71 ± 0.15) was observed relative to the standard drug group (1.76 ± 0.05). A significant (p<0.05) decrease in sperm count and testes weight was also observed in rats treated with A. vogelli extract relative to the normal control.The results suggest that, A. vogelli extract may cause decreased fertility in male albino rats and could be developed further into potent male contraceptives. A. gangetica on the other hand, had no effect on male reproductive hormones.

Sex Hormones and Epididymal Sperm Parameters in Rats Following Sub-chronic Treatment with Hexavalent Chromium

1992 ◽  
Vol 11 (4) ◽  
pp. 255-258 ◽  
Author(s):  
Erik Ernst ◽  
Jens Peter Bonde
Keyword(s):  
Hexavalent Chromium ◽  
Wistar Rats ◽  
Chronic Treatment ◽  
Sperm Count ◽  
Serum Testosterone ◽  
Exposure Period ◽  
Testicular Atrophy ◽  
Epididymal Sperm ◽  
Male Wistar Rats ◽  
High Doses

Testicular atrophy and reduced epididymal sperm count are known to occur after i.p. administration of high doses of hexavalent chromium to rats. The effect of 0.5 mg kg -1 hexavalent chromium injected i.p. 5 d a week for 8 weeks was investigated in male Wistar rats. A significant reduction in epididymal sperm motility was found at the end of the exposure period. The reduction was reversed after an unexposed period of a further 8 weeks. In addition, a decrease in serum testosterone and an increase in FSH were found at the end of the exposure period. The results indicate that a number of mechanisms may be involved in the deleterious effects of chromate on male fecundity.

scholarly journals Effects of Lippia multiflora leaf extract on the semen of Wistar rat

2021 ◽  
Vol 39 (2) ◽  
pp. 172-178
Author(s):  
J. O. Olukunle ◽  
E. S. Ajibola ◽  
K. T. Biobaku ◽  
B. S. Okediran ◽  
O. A. Akinkuotu
Keyword(s):  
Wistar Rats ◽  
Leaf Extract ◽  
Sperm Count ◽  
Experimental Period ◽  
Wistar Rat ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Lippia Multiflora ◽  
Male Wistar Rats ◽  
Dose Dependent

To investigate the effect of Lippia multiflora (Verbanaceae) on the reproductive activity of male Wistar rats, three groups (A,B and C) of rats were treated with 400, 800 and 1600 mg/kg/day of Lippia multiflora leaves extract respectively for 28 days while 5ml/kg of distilled water was given to the control group D. At the end of the experimental period, animals were sacrificed and sperm characteristics, histology of the testes and epididymis were assessed. Lippia multiflora leaf extract caused a significant reduction (P<0.05) in sperm motility, a statistically significant dose dependent reduction in the sperm count and a significant (P<0.05) dose dependent increase in morphological abnormalities of the spermatozoa of the treated rats. Histopathological evaluation of the testis and epididymis revealed varying degrees of degeneration and necrosis of the germinal epithelia cell of the seminiferous tubules, spermiostasis and complete epididymal aspermia. Lippia multiflora leaf extract has significant anti-spermatogenic effects on adult male Wistar rats which could impair reproductive activities in these rats

scholarly journals Aqueous Extract of Allium sativum (Linn.) Bulbs Ameliorated Pituitary-Testicular Injury and Dysfunction in Wistar Rats with Pb-Induced Reproductive Disturbances

2016 ◽  
Vol 4 (2) ◽  
pp. 200-212 ◽  
Author(s):  
Abiodun O. Ayoka ◽  
Aderonke K. Ademoye ◽  
Christian E. Imafidon ◽  
Esther O. Ojo ◽  
Ayowole A. Oladele
Keyword(s):  
Aqueous Extract ◽  
Wistar Rats ◽  
Allium Sativum ◽  
Oral Route ◽  
Reproductive Hormones ◽  
Control Group ◽  
Vascular Congestion ◽  
Male Wistar Rats ◽  
Testicular Injury ◽  
Toxic Group

AIM: To determine the effects of aqueous extract of Allium sativum bulbs (AEASAB) on pituitary-testicular injury and dysfunction in Wistar rats with lead-induced reproductive disturbances.MATERIALS AND METHODS: Male Wistar rats were divided into 7 groups such that the control group received propylene glycol at 0.2 ml/100 g intraperitoneally for 10 consecutive days, the toxic group received lead (Pb) alone at 15 mg/kg/day via intraperitoneal route for 10 days while the treatment groups were pretreated with lead as the toxic group after which they received graded doses of the extract at 50, 100 and 200 mg/kg/day via oral route for 28 days. RESULTS: Pb administration induced significant deleterious alterations in the antioxidant status of the brain and testis, sperm characterization (counts, motility and viability) as well as reproductive hormones (FSH, LH and testosterone) of exposed rats (p < 0.05). These were significantly reversed in the AEASAB-treated groups (p < 0.05). Also, there was marked improvement in the Pb-induced vascular congestion and cellular loss in the pituitary while the observed Pb-induced severe testicular vacuolation was significantly reversed in the representative photomicrographs, following administration of the extract.CONCLUSION: AEASAB treatment ameliorated the pituitary-testicular injury and dysfunction in Wistar rats with Pb-Induced reproductive disturbances.

scholarly journals Effect of dietary supplementation with soybean on reproductive hormones of male Wistar rats

2021 ◽  
Vol 13 (2) ◽  
pp. 134-139
Author(s):  
Yahya Anisah ◽  
I.Y. Mohammed ◽  
M. El-Bashir Jibril
Keyword(s):  
Wistar Rats ◽  
Controlled Trial ◽  
Reproductive Function ◽  
Dietary Supplementation ◽  
Study Groups ◽  
Reproductive Hormones ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Randomised Controlled

Dietary soybean has been shown to have variable effects on the reproductive system of male rodents, non-human primates and humans. These effects can affect subsequent fertility. Soybean intake in men has been advocated due to its protective effect in the prevention of prostate cancer, cardiovascular disease and osteo-arthritis. However, the protection it offers in the prevention of prostatic cancer by altering the levels of male reproductive hormones may lead to infertility. This study aimed at determining the effect of dietary supplementation with soybean on reproductive hormones of male Wistar rats. The study was a randomised controlled trial. Adult male Wistar rats were randomly assigned to a control group and two study groups. The rats in the control group were fed on soy-free diet while rats in Group 1 and Group 2 of the study were fed on 20% and 50% soybean supplemented diet for four weeks. Serum concentration of FSH, LH and Testosterone were estimated. Data obtained was analysed using statistical package for social sciences (SPSS) version 20.0.There was no statistically significant difference (p<0.05) in the level of FSH (p=0.783), LH (p=0.815)and Testosterone (p=0.330) between the study and control groups. Dietary supplementation with of soybean has no effect on the level of FSH, LH and Testosterone in male Wistar rats and therefore may not affect their reproductive function. 

scholarly journals Gestational exposure to the cannabinoid WIN 55,212-2 and its effect on the innate intestinal immune response

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Rosalía Hernández-Cervantes ◽  
Armando Pérez-Torres ◽  
Óscar Prospéro-García ◽  
Jorge Morales Montor
Keyword(s):  
Immune System ◽  
Wistar Rats ◽  
Trichinella Spiralis ◽  
Intestinal Parasites ◽  
Control Group ◽  
Prenatally Exposed ◽  
Mucus Production ◽  
Gestational Exposure ◽  
Male Wistar Rats ◽  
Marijuana Consumption

AbstractThe consequences of marijuana consumption during pregnancy and its effects on the function of the immune system have been little studied. Marijuana is one of the most consumed recreational drugs among pregnant women, and it is known that gestational exposure to marijuana can have serious effects on the offspring after birth. In this study, we challenged the immune system of Wistar rats by infecting them with the parasitic nematode Trichinella spiralis. A treatment group of these animals was prenatally exposed to the cannabinoid WIN 55,212-2; a control group was not exposed. At 5 days of infection, the treated animals were less effective in eliminating intestinal parasites; moreover, this effect was correlated with a deficiency in mucus production, lower recruitment of eosinophils in the duodenum, and a reduced percentage of Tγδ and NK cells. In conclusion, the gestational administration of the synthetic cannabinoid WIN 55,212-2 induces lasting changes to the function of the immune system against infection with T. spiralis in male Wistar rats, making them more susceptible to infection.

scholarly journals Beneficial effects of Cichorium intybus L. extract on oxidative status and reproductive parameters in male Wistar rats: An experimental study

2019 ◽  
Author(s):  
Mehran Dorostghoal ◽  
Seyyed Mansour Seyyednejad ◽  
Marzieh Noroozi Tabrizi Nejad
Keyword(s):  
Experimental Study ◽  
Wistar Rats ◽  
Sperm Count ◽  
Folk Medicine ◽  
Serum Testosterone ◽  
Cichorium Intybus ◽  
Male Rats ◽  
Reproductive Parameters ◽  
Sperm Density ◽  
Male Wistar Rats

Background: During recent years, increasing concern has been raised about the declining sperm count and human male infertility. Cichorium intybus L. (C. intybus) has traditionally been used in Iranian folk medicine as hepato protective and blood purifier and for its presumed fertility-enhancing properties. Objective: A dose-response study was performed to determine the effect ofC. intybus ethanolic leave extract on the reproductive parameters in adult Wistar male rats. Materials and Methods: In this experimental study, 40 healthy adult male Wistar rats (8 wk old, 200-210 gr body weight) were randomly divided (n = 10/each) as control and groups treated with 50, 100, and 200 mg/kg/day of C. intybus extract via gavage for 70 days. Serum hormonal assay, epididymal sperm evaluation, and analysis of morphometrical parameters, antioxidant enzymes, and lipid peroxidation levels of testis were done in each experimental group. Results: Weights of testis and epididymis increase significantly in male rats treated with 200 mg/kg C. intybus extract. Sperm density and percent of morphologically normal sperm were significantly increased in a dose-related manner with C. intybus treatment. Serum testosterone was higher at 100 and 200 mg/kg C. intybus extract-treated groups. C. intybus significantly reduced malondialdehyde levels and also increased superoxide dismutase and glutathione peroxidase activity in testicular tissue of rats. Conclusion: It is concluded that C. intybus leave extract improves reproductive parameters in male rats which might be a consequence of both its antioxidant and androgenic properties.

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