scholarly journals The Potential of Trichoderma sp. as Biological Agent to Support the Germination of Corn Seeds (Zea Mays)

2021 ◽  
Vol 2 (2) ◽  
pp. 27-30
Author(s):  
Betty Lahati ◽  
Zauzah Abdullatif ◽  
Fatmawati Kaddas ◽  
Martini Labaradji
Keyword(s):  
Seed Germination ◽  
Cell Walls ◽  
Seed Viability ◽  
Biological Agents ◽  
Biological Agent ◽  
Seed Coating ◽  
Treatment Control ◽  
Corn Seed ◽  
Trichoderma Sp ◽  
Locally Grown

This research is motivated by the fact that corn seeds in storage are often characterized with low seed viability and infected by diseases so that the seeds grow abnormally and even die. Corn seeds have thick/hard cell walls. It is acknowledged that the corn seed walls contain cellulose, which can be broken down by microorganisms. One such microorganism is the Trichoderma fungus, which is also a biological agent. This study aims to analyze the effect of the biological agents Trichoderma sp. on corn seed germination and identify the best concentration of Trichoderma sp. against corn seed germination. The corn seeds used in this study were locally grown, which had been stored for 6 months. The seed coating (bio seed coating of local corn) was tested at several concentrations of Trichoderma sp. (treatment), namely without treatment (control), 5g, 10g, 15g, and 20g. Each concentration was repeated 4 times, using several parameters to analyze normal sprouts and germination capacity. The analysis results confirmed the potential of Thrichoderma biological agents on corn seed germination. The T1 treatment with 5g Trichoderma biological agents significantly improve corn seed germination.

scholarly journals Seed longevity of maize conserved under germplasm bank conditions for up to 60 years

2021 ◽  
Author(s):  
Filippo Guzzon ◽  
Maraeva Gianella ◽  
Jose Alejandro Velazquez Juarez ◽  
Cesar Sanchez Cano ◽  
Denise E Costich
Keyword(s):  
Seed Germination ◽  
Plant Genetic Resources ◽  
Seed Viability ◽  
Storage Conditions ◽  
Seed Longevity ◽  
Germplasm Bank ◽  
Significant Difference ◽  
Different Temperatures ◽  
Germination Experiments

Abstract Background and Aims The long-term conservation of seeds of plant genetic resources is of key importance for food security and preservation of agrobiodiversity. Nevertheless, there is scarce information available about seed longevity of many crops under germplasm bank conditions. Methods Through germination experiments as well as the analysis of historical monitoring data, we studied the decline in viability manifested by 1000 maize (Zea mays subsp. mays) seed accessions conserved for an average of 48 years at the CIMMYT germplasm bank, the largest maize seedbank in the world, under two cold storage conditions: an active (–3 °C; intended for seed distribution) and a base conservation chamber (–15 °C; for long-term conservation). Key Results Seed lots stored in the active chamber had a significantly lower and more variable seed germination, averaging 81.4 %, as compared with the seed lots conserved in the base chamber, averaging 92.1 %. The average seed viability detected in this study was higher in comparison with that found in other seed longevity studies on maize conserved under similar conditions. A significant difference was detected in seed germination and longevity estimates (e.g. p85 and p50) among accessions. Correlating seed longevity with seed traits and passport data, grain type showed the strongest correlation, with flint varieties being longer lived than floury and dent types. Conclusions The more rapid loss of seed viability detected in the active chamber suggests that the seed conservation approach, based on the storage of the same seed accessions in two chambers with different temperatures, might be counterproductive for overall long-term conservation and that base conditions should be applied in both. The significant differences detected in seed longevity among accessions underscores that different viability monitoring and regeneration intervals should be applied to groups of accessions showing different longevity profiles.

scholarly journals Using Microwave Soil Heating to Inhibit Invasive Species Seed Germination

2017 ◽  
Vol 10 (3) ◽  
pp. 262-270 ◽  
Author(s):  
Mélissa De Wilde ◽  
Elise Buisson ◽  
Nicole Yavercovski ◽  
Loïc Willm ◽  
Livia Bieder ◽  
...  
Keyword(s):  
Invasive Species ◽  
Soil Moisture ◽  
Seed Germination ◽  
Invasive Plant ◽  
Organic Matter Content ◽  
Seed Viability ◽  
Germination Percentage ◽  
Matter Content ◽  
Burial Depth ◽  
Experimental Conditions

Successful invasive plant eradication is rare, because the methods used target the adult stage, not taking into account the development capacity of a large seedbank. Heating by microwave was considered, because it offers a means to quickly reach the temperature required for loss of seed viability and inhibition of germination. Previous results were not encouraging, because homogeneous and deep-wave penetration was not achieved, and the various parameters that can affect treatment effectiveness were incompletely addressed. This study aimed to determine, under experimental conditions, the best microwave treatment to inhibit invasive species seed germination in terms of power (2, 4, 6 kW) and duration (2, 4, 8 min) of treatments and depending on soil moisture (10%, 13%, 20%, 30%) and seed burial depth (2, 12 cm). Three invasive species were tested: Bohemian knotweed, giant goldenrod, and jimsonweed. The most effective treatments required relatively high power and duration (2kW8min, 4kW4min, 6kW2min, and 6kW4min; 4kW8min and 6kW8min were not tested for technical reasons), and their effectiveness diminished with increasing soil moisture with germination percentage between 0% and 2% for the lowest soil moisture, 0% and 56% for intermediate soil moisture, and 27% and 68% in control treatments. For the highest soil moisture, only 2kW8min and 4kW4min reduced germination percentage between 2% and 19%. Occasionally, germination of seeds located at the 12-cm depth was more strongly affected. Giant goldenrod seeds were the most sensitive, probably due to their small size. Results are promising and justify further experiments before developing a field microwave device to treat large volumes of soil infested by invasive seed efficiently and with reasonable energy requirements. Other types of soil, in terms of texture and organic matter content, should be tested in future experiments, because these factors influence soil water content and, consequently, microwave heating.

Tumor Necrosis Factor-blocking Agents for Children with Enthesitis-related Arthritis — Data from the Dutch Arthritis and Biologicals in Children Register, 1999–2010

2011 ◽  
Vol 38 (10) ◽  
pp. 2258-2263 ◽  
Author(s):  
MARIEKE H. OTTEN ◽  
FEMKE H.M. PRINCE ◽  
MARINKA TWILT ◽  
REBECCA ten CATE ◽  
WINEKE ARMBRUST ◽  
...  
Keyword(s):  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Age Of Onset ◽  
Biological Agents ◽  
Biological Agent ◽  
Inactive Disease ◽  
Serious Adverse Events ◽  
Enthesitis Related Arthritis ◽  
Blocking Agents ◽  
Necrosis Factor

Objective.To evaluate the effectiveness and safety of biological agents in children with enthesitis-related arthritis (ERA).Methods.All patients with ERA in whom a biological agent was initiated between 1999 and 2010 were selected from the Dutch Arthritis and Biologicals in Children (ABC) register. In this ongoing multicenter observational register, data on the course of the disease and medication use are retrieved prospectively at the start of the biological agent, after 3 months, and yearly thereafter. Inactive disease was assessed in accordance with the Wallace criteria.Results.Twenty-two patients with ERA started taking 1 or more biological agents: 20 took etanercept, 2 took adalimumab (1 switched from etanercept to adalimumab), and 2 took infliximab (1 switched from etanercept to infliximab). Characteristics: 77% were male, 77% had enthesitis, 68% were HLA-B27-positive. The median age of onset was 10.4 (IQR 9.4–12.0) years; median followup from the start of the biological agent was 1.2 (IQR 0.5–2.4) years. Intention-to-treat analysis shows that inactive disease was achieved in 7 of 22 patients (32%) after 3 months, 5 of 13 patients (38%) after 15 months, and 5 of 8 patients (63%) after 27 months of treatment. Two patients discontinued etanercept because of ineffectiveness, and switched to adalimumab (inactive disease achieved) or infliximab (decline in joints with arthritis after 3 months of treatment). One patient discontinued etanercept because of remission, but had flare and restarted treatment, with good clinical response. No serious adverse events occurred.Conclusion.Tumor necrosis factor (TNF)-blocking agents seem effective and safe for patients with ERA that was previously unresponsive to 1 or more DMARD. However, a sustained disease-free state could not be achieved, and none discontinued TNF-blocking agents successfully.

Purification of a chitinase from Trichoderma sp. and its action on Sclerotium rolfsii and Rhizoctonia solani cell walls.

1997 ◽  
Vol 43 (1) ◽  
pp. 31-37 ◽  
Author(s):  
Luzia H. C. Lima ◽  
Cirano J. Ulhoa ◽  
Adrienne P. Fernandes ◽  
Carlos R. Felix
Keyword(s):  
Rhizoctonia Solani ◽  
Cell Walls ◽  
Sclerotium Rolfsii ◽  
Trichoderma Sp

scholarly journals Non-Deep Physiological Dormancy in Seed and Germination Requirements of Lysimachia coreana Nakai

Horticulturae ◽  
2021 ◽  
Vol 7 (11) ◽  
pp. 490
Author(s):  
Saeng Geul Baek ◽  
Jin Hyun Im ◽  
Myeong Ja Kwak ◽  
Cho Hee Park ◽  
Mi Hyun Lee ◽  
...  
Keyword(s):  
Seed Germination ◽  
Seed Dormancy ◽  
Germination Rate ◽  
Seed Viability ◽  
Cold Stratification ◽  
Dormancy Breaking ◽  
Viability Test ◽  
Physiological Dormancy ◽  
Different Temperatures ◽  
Germination Requirements

This study aimed to determine the type of seed dormancy and to identify a suitable method of dormancy-breaking for an efficient seed viability test of Lysimachia coreana Nakai. To confirm the effect of gibberellic acid (GA3) on seed germination at different temperatures, germination tests were conducted at 5, 15, 20, 25, 20/10, and 25/15 °C (12/12 h, light/dark), using 1% agar with 100, 250, and 500 mg·L−1 GA3. Seeds were also stratified at 5 and 25/15 °C for 6 and 9 weeks, respectively, and then germinated at the same temperature. Seeds treated with GA3 demonstrated an increased germination rate (GR) at all temperatures except 5 °C. The highest GR was 82.0% at 25/15 °C and 250 mg·L−1 GA3 (4.8 times higher than the control (14.0%)). Additionally, GR increased after cold stratification, whereas seeds did not germinate after warm stratification at all temperatures. After cold stratification, the highest GR was 56.0% at 25/15 °C, which was lower than the GR observed after GA3 treatment. We hypothesized that L. coreana seeds have a non-deep physiological dormancy and concluded that 250 mg·L−1 GA3 treatment is more effective than cold stratification (9 weeks) for L. coreana seed-dormancy-breaking.

scholarly journals Early development of corn seedlings primed with synthetic tenorite nanofertilizer

2020 ◽  
Vol 42 ◽  
Author(s):  
Michel Esper Neto ◽  
David W. Britt ◽  
Kyle Alan Jackson ◽  
Alessandro Lucca Braccini ◽  
Tadeu Takeyoshi Inoue ◽  
...  
Keyword(s):  
Seed Germination ◽  
Size Effects ◽  
Seed Priming ◽  
Seedling Development ◽  
Cuo Nanoparticles ◽  
The Other ◽  
Corn Seed ◽  
Cu Toxicity ◽  
Germination And Growth

Abstract: Fertilizer formulation alternatives that avoid unnecessary losses and environmental impacts are being investigated in agricultural management. Seed priming with nanofertilizers prior to planting, reduces concerns about non-target dispersion; however, priming formulations and concentrations must be carefully selected to avoid undesired effects. Here, seed germination and seedling development were evaluated after seed priming with CuO nanoparticles (NPs), CuO bulk and CuCl2. The seeds were immersed in priming solutions of 0, 20, 40, 80 and 160 mg.L−1 Cu for the three Cu sources. Following 8 hours priming, the seeds were evaluated for germination and vigor (first germination count). Root and shoot lengths were measured as well as shoot and root dry biomass. The copper NP did not show any toxic effects on corn seed germination and growth, and also promoted higher biomass when compared to the other Cu sources. On the other hand, CuCl2 primed seeds exhibited Cu-toxicity in roots and shoots for all concentrations tested. Bulk Cu priming results indicated the better role of NPs size effects. These findings support NP-seed priming as an alternative to delivery of essential micronutrients, such as copper, to corn seedlings.

scholarly journals Flowering, Fecundity, Seed Germination, and Seed Viability of Viburnum opulus L. Cultivars

2009 ◽  
Vol 27 (1) ◽  
pp. 31-36
Author(s):  
Janine R. Conklin ◽  
James C. Sellmer
Keyword(s):  
Seed Germination ◽  
Seed Production ◽  
Seed Viability ◽  
Biological Traits ◽  
Limited Information ◽  
Viable Seed ◽  
Invasive Potential ◽  
Short Term ◽  
Viburnum Opulus ◽  
Tetrazolium Test

Abstract Mature specimens of Viburnum opulus and cultivars ‘Leonard's Dwarf’ and ‘Roseum’ were assessed over 2 years for flower and seed production, seed germination, and seed viability as determined by a tetrazolium test to understand their invasive potential. ‘Aureum’, ‘Compactum’, ‘Losely's Compact’, ‘Nanum’, and ‘Xanthocarpum’ were also tested for germination and viability of seeds. Cultivars differed in flower and seed production, seed germination, and seed viability. ‘Roseum’ prolifically produced highly viable seed that germinated at moderate rates under greenhouse conditions (8,354, 100%, and 73%, respectively). Viburnum opulus and ‘Leonard's Dwarf’ produced fewer viable seed which showed moderate to low germination rates (609, 100%, and 53%; 712, 100%, and 5%, respectively). ‘Aureum’ and ‘Xanthocarpum’ seeds germinated at moderate rates (55 and 25%, respectively) and were highly viable (100%). ‘Compactum’, ‘Losely's Compact’, and ‘Nanum’ germinated at low rates or failed to germinate (0, 0, and 5%, respectively), yet seeds were moderately viable (37, 65, and 55%, respectively). Seeds of all cultivars germinated at low rates or failed to germinate at both outdoor sites (0 to 5%) which suggests these plants may be weakly invasive. Short-term studies on biological traits such as these provide only limited information to assess the invasive potential of cultivars.

scholarly journals Peningkatan produktivitas tanaman kedelai kultivar Anjasmoro asal benih terdeteriorasi dengan kompos Trichoderma dan bokashi

Kultivasi ◽  
2018 ◽  
Vol 17 (3) ◽  
Author(s):  
Sumadi Sumadi ◽  
Denny Sobardini Sobarna ◽  
Pujawati Suryatmana ◽  
Meddy Rachmadi ◽  
Erni Suminar
Keyword(s):  
Plant Growth ◽  
Paddy Soil ◽  
Biological Agents ◽  
Seed Vigor ◽  
Pot Experiment ◽  
Seed Coating ◽  
Trichoderma Spp ◽  
Dry Land ◽  
Better Than

Sari. Trichoderma spp merupakan golongan fungi yang bersifat antipatogen, khususnya layu kecambah, sedangkan bokashi merupakan kompos yang diperkaya dengan beberapa mikroba yang bermanfaat bagi pertumbuhan tanaman.Tulisan ini merupakan rangkuman penelitian pot menggunakan kompos Trichoderma spp sebagai pelapis benih terdeteriorasi disertai bokashi kotoran hewan yang dilakukan pada tanah lahan kering dan tanah sawah pada tahun 2014 dan 2017. Percobaan pertama membandingkan efektivitas beberapa agen hayati sebagai pelapis benih pengaruhnya terhadap vigor benih dan hasil tanaman. Hasil percobaan menunjukkan penggunaan kompos Trichoderma hasilnya lebih baik dibandingkan agen hayati lainnya. Hasil percobaan selanjutnya menunjukkan  bahwa pelapisan benih dengan 1 sampai 3 g  kompos Trichoderma spp per 100 butir benih pengaruhnya tidak nyata dibandingkan dengan penggunaan pelapis benih berupa pestisida, baik terhadap vigor  maupun hasil biji per tanaman. Pemberian bokashi sebaliknya secara nyata mampu meningkatkan hasil biji per tanaman . Pemberian bokashi 15 t ha-1menghasilkan biji seberat 19.83 g per tanaman  atau setara dengan 2,379 t ha-1 atau 27,3 % lebih tinggi dari kontrol.Kata kunci : Benih terdeteriorasi, pelapisan benih, Trichoderma, Bokashi Abstract. Trichoderma spp is a group of antipatogenic fungi, especially seedling wilted, while bokashi is a compost enriched with some microbes that are beneficial to plant growth. This paper is a compilation of pot experiment using Trichoderma compost for coating deteriorated seed and accompanied by bokashi on dry land soil and paddy soil in 2014 and 2017 respectively. The first experiment compares the effectiveness of several biological agents as a seed coating of its effect on seed vigor and yield. The results of first experiment showed that the use of 2 g Trichoderma compost 100 seeds-1 was better than other biological agents. However between 1.2 and 3 g of Trichoderma compost per 100 seeds was not significant effect on seed vigor and yield. The results of experiment showed that the coating of seeds with 1 – 3 g of Trichoderma spp compost 100 compared with pesticide seed coatings. Increased of yield only affected bokashi application. Application of bokashi 15 t ha-1 abble to produce 19.83 g seed per plant or equivalent to 2.379 t ha-1 or 27.3% higher than the control.Key words: deteriorated seeds, seed coating, Trichoderma, bokashi

scholarly journals PENGARUH KONSORSIUM AGENS HAYATI DAN JARAK TANAM TERHADAP KEJADIAN PENYAKIT BUSUK PUTIH (Sclerotium cepivorum Berk.) PADA BAWANG DAUN Influence of The Consortium of Biological Agents and Spacing to White Rot Disease (Sclerotium cepivorum Berk.) of Scallio

AgriPeat ◽  
2019 ◽  
Vol 20 (02) ◽  
pp. 92-98
Author(s):  
Admin Journal
Keyword(s):  
Block Design ◽  
Peat Soil ◽  
Nutrient Content ◽  
Biological Agents ◽  
White Rot ◽  
Plant Weight ◽  
Sclerotium Cepivorum ◽  
Trichoderma Sp ◽  
Rot Disease ◽  
Aspergillus Sp

ABSTRACTThis study aims to determine effective management patterns to control Sclerotium rot and to improvepeat soil fertility with the application of the Trichoderma sp + Aspergillus sp consortium.as biologicalagents and P solvents, combined with spacing arrangements in the cultivation of scallion in peatlands.The study used a factorial randomized block design consisting of two factors with four replications.Factor I Application of biological agents consists of: A0 = No biological agents; A1 = Consortium ofTrichoderma sp. + Aspergillus sp; Factor II Plant spacing, consisting of J1 = 20x25 cm; J2 = 25x25cm and J3 = 30x25 cm. The results showed that the interaction treatment of Trichoderma sp. +Aspergillus sp. and spacing of 25x25 cm effectively suppresses the incidence of white rot disease(Sclerotium cepivorum Berk) up to 34.02%, while at a spacing of 20x25 cm with the application ofTrichoderma sp. + Aspergillus sp. produced the highest fresh plant weight of 8.80 kg plot-1 or 24.44tons hectares-1. Increasing the number of leaves is only influenced by a single factor of biologicalagents (23.29%) and spacing of 25x25 cm (19.7%). Application of Trichoderma sp. + Aspergillus sp.can increase the nutrient content of N, P (total and available), K and peat soil organic matter.Consortium of biological agents Trichoderma sp. and Aspergillus sp. indigenous have the potential tobe developed as biological agents and biofertilizers, with optimum spacing can be applied to themanagement of scallion cultivation in peatlands.Key words: Sclerotium cepivorum Berk, scallion, Trichoderma sp. and Aspergillus sp., spacing

scholarly journals Pemberdayaan Kelompok Tani Hortikultura di Lahan Pasir melalui Pemanfaatan Kayambang (Salvinia molesta) sebagai Trichokompos

2021 ◽  
Vol 6 (4) ◽  
pp. 369-375
Author(s):  
Rahmawati Budi Mulyani ◽  
Lilies Supriati ◽  
Melhanah Melhanah ◽  
Susi Kresnatita
Keyword(s):  
Control Plant ◽  
Biological Agents ◽  
Plant Diseases ◽  
Organic Fertilizers ◽  
Mentoring Program ◽  
Salvinia Molesta ◽  
Horticultural Crop ◽  
Self Help ◽  
Trichoderma Sp ◽  
Farmer Groups

Lebak swamp weeds such as Kayambang (Salvinia molesta) grow abundantly. They can be used as compost, which effectively improves soil fertility, increasing nutrients N, P, and P K quickly and environmentally friendly. The effectiveness of compost fertilizer needs to be increased by adding indigenous microbes as decomposers and biological agents to control plant diseases. The activities carried out to empower horticultural farmer groups on sandy land in Tanjung Pinang Village, Palangka Raya are through socialization, training in composting with three types of antagonist fungus Trichoderma sp. (Trichocompost), facilitate the procurement of weed chopping machines, assist farmers in horticultural crop cultivation, and increase farmers' independence in self-supporting organic fertilizers. The use of Kayambang as Trichocompost with microbial decomposers and indigenous biological agents is new knowledge for partner farmers. The application of Trichocompost on the demonstration plots shows that eggplant plant growth and yields are excellent, meaning that Trichocompost can improve the fertility of sandy soils. Farmers participating in the training stated that the use of Trichocompost could reduce farming costs because it can substitute for manure that has been used by farmers and can meet the self-help needs of organic fertilizers. Participants wanted an advanced mentoring program because the farmers had not yet mastered the isolation or propagation of biological agents and decomposer microbes.

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