scholarly journals JUMLAH KOLONI CANDIDA ALBICANS PADA PLAT RESIN AKRILIK HEAT CURED SETELAH DILAKUKAN PERENDAMAN EKSTRAK DAUN KEMANGI (OCIMUM BASSILICUM LINN) 50%

2020 ◽  
Vol 16 (2) ◽  
pp. 61-63
Author(s):  
Kadek Ayu Wirayuni ◽  
◽  
Sintha Nugrahini
Keyword(s):  
Candida Albicans ◽  
Leaf Extract ◽  
Acrylic Resin ◽  
The Body ◽  
Control Group ◽  
Control Group Design ◽  
Denture Stomatitis ◽  
Parametric Test ◽  
Group Design ◽  
Cleaning Methods

Accumulation of plaque and food scraps on an acrylic resin base will increase bacterial colonies and C. albicans which will cause denture stomatitis. Maintenance of denture hygiene using mechanical, chemical and combination of two methods. Basil leaf extract contain essential oils which are important in fight against resistant C. albicans biofilms. The purpose of this study was to determine the comparison of C. albicans colonies after cleaning various denture cleaning methods. The method of this study is used an experimental method, the study design is posttest only with control group design. Sample size of heat-cured acrylic resin plate is 40x12x3mm. this study use Kruskall-Wallis test and Mann-Whitney test as data analysis for comparison tests between groups (non-parametric test). The results showed that there were significant differences between the various cleansing methods used in reducing C. albicans colonies. The compotition of flavonoids basil leaf extract is anti-microbial which can prevent the entry of fungi that harm the body.

DIFFERENCES IN THE NUMBER OF CANDIDA ALBICANS COLONIES ON ACRYLIC RESIN AND THERMOPLASTIC NYLON IN SOURSOP LEAF EXTRACT IMMERSION

2021 ◽  
Vol 17 (2) ◽  
pp. 123-131
Author(s):  
Ria Koesomawati ◽  
Keyword(s):  
Candida Albicans ◽  
Leaf Extract ◽  
Acrylic Resin ◽  
Wilcoxon Test ◽  
Control Group ◽  
Control Group Design ◽  
Test Analysis ◽  
Denture Stomatitis ◽  
Treatment Groups ◽  
Significant Difference

Introduction: Denture stomatitis is a chronic inflammation caused by wearing dentures, mainly caused by Candida albicans. Heat-polymerized acrylic resins are often used as denture bases, but lack the ability to absorb liquids due to their porosity and surface roughness. The basis of the latest dentures is thermoplastic nylon because it is more aesthetically, hypoallergenic and more flexible. Soursop leaf extract contains alkaloids, tannins, flavonoids, and saponins as antifungal. The purpose of this study was to examinate whether there were differences in the number of Candida albicans colonies on heat-polymerized acrylic resin plates and thermoplastic nylon in soursop leaf extract immersion. Materials and Methods: The research design was an experimental pre-post test with control group design, n = 50 plates were divided into 2 groups, heat-polymerized acrylic resin (n=25) and thermoplastic nylon (n=25), divided into 5 groups, 3 treatment groups using 10%,15%,25% soursop leaf extract, Fittident®and aquadest in the control groups. Samples were contaminated with Candida albicans suspension and incubated, counted before immersion for 8 hours, then put in Saboroud’s bath and counted again. Results and Discussions: Wilcoxon test analysis showed significant differences in all groups, except the aquadest group. The Mann-Whitney test showed significant differences between the treatment groups, except between the 25% extract and the Fittident®control group. Also, showed a significant difference in the 15% concentration group between heat-polymerized acrylic resin and thermoplastic nylon. Conclusion:There was a difference in the decrease in the number of Candida albicans colonies on heat-polymerized acrylic resin plates and thermoplastic nylon in soursop leaf extract at a concentration of 15%, while at concentrations of 10% and 25% there was no difference

scholarly journals Pengaruh jumlah glass fiber non dental pada reinforced resin akrilik (polimetil metakrilat) terhadap perlekatan Candida albicansEffect of the concentration of non-dental glass fiber on reinforced acrylic resin (polymethyl methacrylate) on the attachment of Candida albicans

2019 ◽  
Vol 31 (2) ◽  
Author(s):  
Intan Mardhiyah Jaelani ◽  
Widya Puspita Sari ◽  
Okmes Fadriyanti
Keyword(s):  
Candida Albicans ◽  
Glass Fiber ◽  
Acid Resistance ◽  
Acrylic Resin ◽  
Control Group ◽  
P Value ◽  
Fiber Reinforced ◽  
Average Yield ◽  
Control Group Design ◽  
Group Design

Pendahuluan: Glass fiber non dental merupakan bahan yang digunakan sebagai penguat gipsum dan komponen otomotif dengan komponen yaitu Na2O dan K2O dapat meningkatkan ketahanan terhadap asam dan meningkatkan penyerapan air. Glass fiber non dental banyak tersedia di Indonesia dengan harga yang terjangkau dapat menjadi alternatif pengganti dari E-glass fiber dental yang ketersediaannya terbatas di Indonesia dan harga relatif mahal. Tujuan penelitian adalah menganalisis pengaruh jumlah glass fiber non dental pada reinforced resin akrilik terhadap perlekatan C.albicans. Metode: Jenis penelitian yaitu Eksperimen laboratorium dengan rancangan Posttest Control Group Design  yang dilakukan untuk mengetahui pengaruh jumlah glass fiber non dental pada reinforced resin akrilik terhadap perlekatan C. albicans  dengan 12 sampel yang dikelompokkan menjadi 3 kelompok yaitu glass fiber non dental 0,9 %, glass fiber non dental 1,8 %, dan tanpa fiber. Analisis statistik menggunakan uji Oneway ANOVA dengan nilai p < 0,05. Hasil:  penelitian menunjukkan bahwa glass fiber non dental 0,9 % ( hasil rata-rata: 1,10),  glass fiber non dental 1,8 % (hasil rata-rata: 1,125), dan tanpa fiber (hasil rata-rata: 1,525) memiliki efek berbeda tetapi tidak bermakna. Simpulan: Penambahan glass fiber non dental pada lempeng resin akrilik tidak berpengaruh terhadap perlekatan C. albicans.Kata kunci: Glass fiber non dental,  fiber reinforced resin akrilik, Candida albicans ABSTRACTIntroduction: Non-dental glass fiber is a material used as gypsum reinforcement and automotive components with components namely Na2O and K2O can increase acid resistance and increase water absorption. Non-dental glass fiber is widely available in Indonesia at an affordable price can be an alternative substitute for E-glass dental fiber whose availability is limited in Indonesia and the price is relatively high. The purpose of this study was to analyse the effect of the amount of non-dental glass fiber on reinforced acrylic resin on the attachment of C. albicans. Methods: This type of research is a laboratory experiment with a Posttest Control Group Design that was conducted to determine the effect of the number of non-dental glass fiber on reinforced acrylic resin on the attachment of C. albicans with 12 samples grouped into 3 groups, namely 0.9% non-dental glass fiber, non dental glass fiber 1.8%, and without fiber. Statistical analysis using the Oneway ANOVA test with a p-value <0.05. Results: The study showed that non-dental glass fiber was 0.9% (average yield: 1.10), non-dental glass fiber was 1.8% (average yield: 1,125), and without fiber (average yield: 1,525) has a different but not significant effect. Conclusion: Addition of non-dental glass fiber to acrylic resin plates did not affect the attachment of C. albicans.Keywords: Non-dental glass fiber, fiber-reinforced acrylic resin, Candida albicans

scholarly journals Uji Konsentrasi Hambat Minimum (KHM) Ekstrak Daun Mengkudu (Morinda citrifolia L.) terhadap Candida albicans Secara In Vitro

e-GIGI ◽  
2017 ◽  
Vol 5 (2) ◽  
Author(s):  
Lisa Ramschie ◽  
Pieter L. Suling ◽  
Krista V. Siagian
Keyword(s):  
Candida Albicans ◽  
Minimum Inhibitory Concentration ◽  
Leaf Extract ◽  
Inhibitory Concentration ◽  
Morinda Citrifolia ◽  
Control Group ◽  
Dilution Method ◽  
Control Group Design ◽  
Group Design

Abstract: Noni (Morinda cittrifolia L.) leaves contain antraquinon, atsiri oil, saponin, tannin, alkaloid, flavonoid, polifenol, and sterol that have been proved can inhibit the growth of Candida albicans. This study was aimed to establish the minimum inhibitory concentration (MIC) of noni leaf extract against Candida abicans. This was a true experimental study with a randomized pretest-posttest control group design. We used serial dilution method with turbidimetry and spectrophotometry tests. Noni leaves were extracted by using maceration with 96% ethanol. Candida albicans fungi were obtained from Microbiology Laboratory of Faculty of Mathematics and Natural Sciences, Sam Ratulangi University. The turbidimetry test using three repetitions showed that the MIC of noni leaf extract against Candida albicans was 6.25% meanwhile the spectrophotometry test established 12.5% as the MIC of noni leaf extract. Conclusion: Minimum inhibitory concentration of noni (Morinda cittrifolia L.) leaf extract against the growth of Candida albicans was 12.5%.Keywords: noni (Morinda citrifolia L.), Candida albicans, minimum inhibitory concentration (MIC) Abstrak: Daun mengkudu (Morinda citrifolia L.) mengandung antraquinon, minyak atsiri, saponin, tannin, alkaloid, flavonoid, polifenol dan sterol yang terbukti dapat menghambat pertumbuhan Candida albicans. Penelitian ini bertujuan untuk mendapatkan konsentrasi hambat minimum (KHM) dari ekstrak daun mengkudu terhadap Candida albicans. Jenis penelitian ialah eksperimental murni dengan randomized pretest-posttest control group design. Metode yang digunakan dalam penelitian yaitu serial dilusi dengan pengujian turbidimetri dan spektrofotometri. Daun mengkudu diekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Jamur Candida albicans diambil dari stok jamur Laboratorium Mikrobiologi Program Studi Farmasi Fakultas MIPA Universitas Sam Ratulangi. Hasil penelitian menunjukkan bahwa pengujian turbidimetri dengan tiga kali perlakuan mendapatkan KHM pada konsentrasi 6,25% sedangkan pengujian spektrofotometri mendapatkan KHM pada konsentrasi 12,5%. Simpulan: Konsentrasi hambat minimum (KHM) ekstrak daun mengkudu (Morinda citrifolia L.) terhadap pertumbuhan Candida albicans terdapat pada konsentrasi 12,5%.Kata kunci: mengkudu (Morinda citrifolia L.), Candia albicans, konsentrasi hambat minimum (KHM)

scholarly journals EFEKTIVITAS KOMBINASI EKSTRAK DAUN SIRIH MERAH (Piper Crocatum) DAN EKSTRAK BIJI ALPUKAT (Persea americana) DALAM MENGHAMBAT PERTUMBUHAN Candida albicans

2017 ◽  
Vol 2 (2) ◽  
pp. 86 ◽  
Author(s):  
Vini Anggraini ◽  
Masfufatun Masfufatun
Keyword(s):  
Candida Albicans ◽  
Leaf Extract ◽  
Seed Extract ◽  
Persea Americana ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Betel Leaf ◽  
Post Test ◽  
Avocado Seed

Abstrak   Candida albicans merupakan salah satu mikrooganisme penyebab infeksi kandidiasis. Daun sirih merah (Piper crocatum) dan biji alpukat (Persea americana) telah diteliti dapat menghambat pertumbuhan Candida albicans. Tujuan penelitian ini adalah untuk mengetahui efektivitas kombinasi daun sirih merah (Piper crocatum) dan ekstrak biji alpukat (Persea americana) dalam menghambat pertumbuhan Candida albicans. Penelitian bersifat eksperimental laboratorium (true experiment) dengan pendekatan post test control group design only. Daun sirih merah dan biji alpukat diekstraksi secara maserasi menggunakan pelarut etanol.    Pada masing-masing ekstrak etanol dilakukan uji pendahuluan untuk mengetahui konsentrasi optimum ektrak dalam memnghambat pertumbuhan C. albicans. Daya Hambat pertumbuhan C. albicans diuji menggunakan metode difusi. Hasil penelitian menunjukkan bahwa ekstraks daun sirih merah dan biji alpukat masing-masing memiliki daya hambat terbesar pada konsentrasi 10%. Kombinasi ekstrak daun sirih merah dan ekstrak biji alpukat memiliki daya hambat yang signifikan terhadap pertumbuhan Candida albicans dibandingakan kontrol negatif, kontrol positif dan ekstrak daun sirih merah. Dengan demikian kombinasi ekstrak daun sirih dan biji aplukat etanol berpotensi sebagai antifungi dalam menghambat pertumbuhan C. albicans sehingga diharapkan bisa mengurangi prevalensi kandidiasis. Kata Kunci :    daun sirih merah,  biji alpukat, Candida albicans,                                                           Abstract Candida albicans is one of the microbialism that causes candidiasis infection. Red betel leaf (Piper crocatum) and avocado seed (Persea americana) have been studied to inhibit the growth of C. albicans. The purpose of this study was to determine the effectiveness of red betel leaf (Piper crocatum) and avocado seed extract (Persea americana) combination in inhibiting the growth of C. albicans. Research is experimental laboratory (true experiment) with post test approach control group design only. Red betel leaf and avocado seeds are extracted by maceration using ethanol solvent. In each ethanol extract, a preliminary test was performed to find out the optimum concentration of extract in inhibiting the growth of C. albicans. C. albicans growth retardant was tested using diffusion method. The results showed that the extract of red betel leaf and avocado seeds each had the greatest inhibitory concentration at 10%. The combination of red betel leaf extract and avocado seed extract have significant inhibitory effect on C. albicans growth compared to negative control, positive control and red betel leaf extract. Thus, the combination of betel leaf extract and ethanol  seed has the potential as an antifungal in inhibiting the growth of C. albicans so it is expected to reduce the prevalence of candidiasis. Keywords: red betel leaf, avocado seed, Candida albicans, 

scholarly journals Uji daya hambat rebusan daun pepaya (carica papaya) terhadap pertumbuhan Candida albicans pada plat resin akrilik polimerisasi panas

e-GIGI ◽  
2017 ◽  
Vol 5 (1) ◽  
Author(s):  
Nurul A. Suni ◽  
Vonny N. S. Wowor ◽  
Michael A. Leman
Keyword(s):  
Candida Albicans ◽  
Carica Papaya ◽  
Negative Control ◽  
Control Group ◽  
Control Group Design ◽  
Denture Stomatitis ◽  
Group Design ◽  
Acrylic Plate ◽  
Removable Denture ◽  
Post Test

Abstract: Unclean acrylic removable denture will be the gathering place of plaques that further become media for the growth of microorganisms, inter alia Candida albicans. Abnormal growth of fungi could result in denture stomatitis. Prevention can be done by using cleaning materials, however, these materials contain many chemical substances and are relatively expensive. Carica papaya is one of the herbs that contain active compounds which are antifungal. This study was aimed to determine the inhibition effect of papaya leaves on the growth of C. albicans isolated from the hot plate acrylic resin polymerization. This was a pure experimental study with a post test only control group design. Absorbance values were obtained by using a standard spectrophotometer with Mc Farland No. 1. The absorbance values were incorporated into Stainer formula to determine the total number of colonies of C. albicans. The results of the converted absorbance values were as follows: the papaya leaf 0.51 x 108 CFU; the positive control (polident) 2.5 x 108 CFU; and the negative control (sterile distilled water) 3.6 x 108 CFU. Conclusion: Papaya leaves (Carica papaya) had inhibitory effect on the growth of Candida albicans.Keywords: papaya leaf (Carica papaya), removable denture acrylic plate, Candida albicansAbstrak: Kebersihan gigi tiruan lepasan akrilik yang kurang diperhatikan akan menjadi tempat berkumpulnya plak yang dapat menjadi media untuk bertumbuhnya mikroorganisme, antara lain Candida albicans. Pertumbuhan jamur yang abnormal dapat mengakibatkan denture stomatitis. Pencegahan dapat dilakukan dengan menggunakan bahan pembersih, namun pembersih yang beredar saat ini banyak mengandung bahan kimia dan harga yang relatif mahal. Daun pepaya (Carica papaya) merupakan salah satu tanaman herbal yang mengandung senyawa aktif yang bersifat antifungi. Penelitian ini bertujuan untuk mengetahui daya hambat rebusan daun pepaya terhadap pertumbuhan C. albicans yang diisolasi dari plat resin akrilik polimerisasi panas. Jenis penelitian ialah eksperimental murni dengan post test only control group design. Nilai absorbansi diperoleh dengan menggunakan spektrofotometer standar Mc Farland no 1, kemudian nilai absorbansi dimasukan ke dalam rumus Stainer untuk mengetahui jumlah total koloni C. albicans. Hasil penelitian menunjukkan nilai absorbansi setelah dikonversikan ke dalam rumus ialah pada rebusan daun pepaya 0,51 x 108 CFU; kontrol positif (polident) 2,5 x 108 CFU; dn kontrol negatif (akuades steril) 3,6 x 108 CFU. Simpulan: Air rebusan daun pepaya (Carica papaya) memiliki daya hambat terhadap pertumbuhan Candida albicans.Kata kunci: daun pepaya (carica papaya), plat gigi tiruan

scholarly journals The Effectivity of Parsley (Petroselinum crispum) Extract on The Growth Inhibition of Candida Albicans

2021 ◽  
Vol 7 (3) ◽  
pp. 123-128
Author(s):  
Rafinda Naditya Arismunandar ◽  
Isniya Nosartika ◽  
Brigitta Natania Renata Purnomo ◽  
Arlita Leniseptaria Antari
Keyword(s):  
Candida Albicans ◽  
Ethanol Extract ◽  
Acrylic Resin ◽  
Etiologic Agent ◽  
Control Group ◽  
Petroselinum Crispum ◽  
Control Group Design ◽  
Denture Stomatitis ◽  
Inhibition Of Growth ◽  
Post Test

Background: Denture stomatitis is a type of Candida-associated infection that mainly affects the palatal mucosa. Candida albicans is one of the normal flora that is considered to be the primary etiologic agent in the pathogenesis of denture stomatitis. Denture decontamination is necessary to prevent denture stomatitis. One method of decontamination is by immersing removable dentures in an antifungal solution. Parsley (Petroselinum crispum) is a medicinal plant showing antifungal activity. Objectives: To determine the effectivity of immersion of acrylic resin Plate in parsley extract on inhibition of growth of Candida albicans. Methods: The effectivity of immersion of heat-cured acrylic resin plates in parsley extract on inhibition of C. albicans growth was tested using an experimental laboratory study with a post-test-only control group design. Thirty samples were divided into 5 groups as 0.01%, 0.02%, and 0.05% parsley ethanol extract, 0.1% sodium hypochlorite, and sterile aquadest.  The number of colony forming units per mL was obtained after calculating the colonies on SDA media, allowing the minimum inhibitory concentration (MIC) obtained according to the formula. Results: 0.01% parsley ethanol extract is MIC with a mean of 7.4 CFU / ml, which inhibited the growth of C. albicans by 31.05%. The Kruskal Wallis test (p<0.001) results indicate that there is an effectivity of immersion of acrylic resin plate in parsley extract on inhibition of growth of C. albicans. Conclusions: The immersion of acrylic resin plates in parsley ethanol extract with a concentration of 0.01%, 0.02%, and 0.04% has effectivity on the inhibition of growth of C. albicans.

scholarly journals Efektivitas ekstrak kulit kayu rambutan (Nephelium lappaceum L.) terhadap Candida albicansThe effectiveness of rambutan (Nephelium lappaceum L.) bark extract on the growth of Candida albicans

2021 ◽  
Vol 33 (2) ◽  
pp. 139
Author(s):  
Masdelina Nasution ◽  
Minasari Nasution ◽  
Mirza Hasibuan ◽  
Yumi Lindawati
Keyword(s):  
Candida Albicans ◽  
Bark Extract ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Nephelium Lappaceum ◽  
Post Test

Pendahuluan: Ekstrak kulit kayu rambutan memiliki senyawa aktif yang digunakan sebagai  antijamur terutama pada Candida albicans. Candida albicans pada rongga mulut dapat menyebabkan kandidiasis lidah. Salah satu faktor predisposisi yang memicu kandidiasis adalah terganggunya ekologi mulut atau perubahan mikrobiologi mulut karena pemakaian antibiotika dalam waktu yang lama oleh karena itu diperlukan antibiotik berbahan herbal yang dapat digunakan secara topikal. Tujuan penelitian ini untuk mengetahui efektivitas daya hambat ekstrak kulit kayu rambutan terhadap pertumbuhan Candida albicans pada konsentrasi 80%, 40%, 20%,10%, konsentrasi minimum daya hambat (KHM) dan daya bunuh (KBM) ekstrak kulit kayu rambutan terhadap Candida albicans. Metode: Penelitian ini merupakan eksperimental laboratoris dengan rancangan penelitian post test only control group design. Sampel yang digunakan adalah Candida albicans yang diisolasi dari penderita kandidiasis lidah dan dibiakkan di Laboratorium Mikrobiologi RS USU. Pengujian efektivitas ekstrak kulit kayu rambutan terhadap pertumbuhan Candida albicans dengan metode dilusi dan difusi, ekstrak kulit kayu rambutan dibuat dengan teknik maserasi menggunakan pelarut etanol 70% dengan berbagai konsentrasi (80%, 40%, 20% dan 10%). Hasil: uji Kruskal- Wallis menunjukkan perbedaan zona hambat yang signifikan pada beberapa konsentrasi. KHM Ekstrak kulit kayu rambutan diperoleh 20%, dan KBM 40%. Simpulan: bahwa ekstrak kulit kayu rambutan memiliki efektivitas antijamur terhadap Candida albicans.Kata kunci: kulit kayu rambutan, KHM, KBM, Zona hambat, Candida albicans.

scholarly journals Efektivitas Ekstrak Bawang Merah (Allium ascalonicum L) Terhadap Penurunan Suhu Tubuh Anak dengan Demam Pasca Imunisasi DPT Pentabio

2021 ◽  
Vol 11 (1) ◽  
pp. 6
Author(s):  
Setiawandari Setiawandari ◽  
Eka Deviany Widyawaty
Keyword(s):  
Body Temperature ◽  
Hepatitis B ◽  
The Body ◽  
Control Group ◽  
P Value ◽  
Type B ◽  
Control Group Design ◽  
Group Design ◽  
Influenza Type ◽  
Post Test

The immunizations that must be given to children include the Diphtheria Pertussis Tetatus-Hepatitis B-Hemophilus Influenza type B vaccine or better known as Pentabio DPT. The DTwP / HB / HiB vaccine is given to babies in 3 doses at 1 month intervals. This immunization caused a systemic reaction, one of which was a fever of 0.85% in the first 30 minutes and increased to 14.03% on the day after immunization. The purpose of this study was to determine the effectiveness of shallots (Allium ascalonicum L) in reducing the body temperature of feverish children after Pentabio DPT immunization. This type of research was quasi-experimental study, with pre and post test with control group design. The sample in this study were babies who received DPT immunization as many as 20 respondents. DPT Pentabio immunization was given a dose of 0.5 ml in the intervention group and the control group. The statistical test used was the T test. The results showed that there was a significant effect of shallots on in reducing the body temperature of feverish children after Pentabio DPT immunization, with a p-value of 0.000. Keywords: shallots; body temperature; fever; Pentabio DPT immunization ABSTRAK Imunisasi yang wajib diberikan kepada anak-anak diantaranya adalah vaksin Diphtheria Pertusis Tetatus-Hepatitis B-Hemophilus Influenza type B atau lebih dikenal dengan DPT Pentabio. Vaksin DTwP/HB/HiB diberikan kepada bayi sebanyak 3 dosis dengan interval 1 bulan. Imunisasi ini menimbulkan reaksi sistemik salah satunya berupa demam 0,85% pada 30 menit pertama dan meningkat menjadi 14,03% pada satu hari pasca imunisasi. Tujuan dari penelitian ini adalah mengetahui efektifitas bawang merah (Allium ascalonicum L) terhadap penurunan suhu tubuh anak demam pasca imunisasi DPT Pentabio. Jenis penelitian quasi eksperimen dengan pre and post test with control group design. Sampel pada penelitian ini adalah bayi yang mendapat imunisasi DPT sebanyak 20 orang responden. Pemberian imunisasi DPT PENTABIO Pentabio dosis 0,5 ml pada kelompok intervensi dan kelompok kontrol. Uji statistik yang digunakan adalah Uji T. Hasil penelitian menunjukkan ada pengaruh yang signifikan dari bawang merah dalam menurunkan suhu tubuh anak demam pasca imunisasi DPT Pentabio, dengan p-value 0,000. Kata kunci: bawang merah; suhu tubuh; demam; imunisasi DPT Pentabio

scholarly journals PENGARUH DESINFEKSI CETAKAN FISIOLOGIS DENGAN MICROWAVE DAN SODIUM HIPOKLORIT TERHADAP JUMLAH CANDIDA ALBICANS DAN STABILITAS DIMENSI MODEL KERJA GIGI TIRUAN CEKAT

2019 ◽  
Vol 12 (3) ◽  
pp. 322-328
Author(s):  
Putri Welda Utami Ritonga ◽  
Bayu Panca Nugraha
Keyword(s):  
Stainless Steel ◽  
Candida Albicans ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Test

Bahan cetak polivinil siloksan(PVS) sering digunakan pada pencetakan untuk pembuatan gigi tiruan cekatkarena mampu menghasilkan cetakan yang akurat dengan dimensi cetakan yang stabil serta dapat disimpan dalam waktu lama. Namun, pencetakan ini tidak terlepas dengan hubungannya terhadap rongga mulut dan mikroorganismenya yang dapat menimbulkan infeksi silang.Candida albicansmerupakan jenis mikroorganisme yang sering ditemukan melekat pada permukaan cetakan. Pemilihan cara desinfeksi penting dalam memperoleh keberhasilan desinfeksi Candida albicansserta mempertahankan stabilitas dimensi hasil cetakan. Penelitian ini bertujuan untuk mengetahui pengaruh desinfeksi cetakan fisiologis dengan microwave dan sodium hipoklorit terhadap jumlah Candida albicansdan stabilitas dimensi model kerja gigi tiruan cekat. Penelitian ini dilakukan pada sampel berupa cetakan yang didapat dari pencetakan model induk yang terbuat dari stainless steel berbentuk silindris dengan tinggi 3 mm dan diameter 29,97 mm serta memiliki 3 takik horizontal dengan jarak 2,5 mm dan 2 takik vertikal dengan jarak 25,02 mm dengan kedalaman 500 μm untuk uji jumlah Candida albicans, dan model kerja yang didapat dari pengisian cetakan dengan bahan gips keras tipe IV untuk uji stabilitas dimensi. Rancangan penelitian adalah eksperimental laboratoris dengan post test only control group design.30 sampel hasil cetakan PVS digunakan untuk menghitung jumlah Candida albicansdan 30 sampel model kerja terbuat dari gips tipe IV digunakan untuk pengukuran stabilitas dimensi. Data yang diperoleh dianalisis dengan uji Kruskal-Wallis. Hasil penelitian menunjukkan ada pengaruh desinfeksi cetakan fisiologis dengan microwavedan sodium hipoklorit terhadap jumlah Candida albicansdan stabilitas dimensi model kerja.

scholarly journals Pengaruh pemberian vitamin E terhadap kadar neutrofil setelah latihan fisik

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Kristo Warong ◽  
Damajanty H.C. Pangemanan ◽  
Joice N.A. Engka
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Physical Exercise ◽  
Tissue Injury ◽  
The Body ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
Test Control

Absract: Physical exercise can improve and maintain physical fitness. However, physical exercise can lead to oxidative stress that can reduce the activity of antioxidants. Vitamin E is a fat-soluble antioxidant that could free radicals in the body. Neutrophils play an active role in the process of phagocytosis of bacteria and other microorganisms the damaged tissue caused by tissue injury. This study was aimed to obtain the effect of vitamin E on neutrophil count after physical exercise. This was a field experimental study with a pre post test control group design. Data were analyzed with the Mann Whitney U (α = 0.05). The physical exercise was playing futsal for 60 minutes. Respondents were 30 male respondents divided into 2 groups: treatment and control groups. The levels of neutrophils were examined after physical exercise and after the administration of vitamin E 400 IU for seven days. Data analysis of the effect of vitamin E on neutrophil level after physical exercise showed a p value of 0.031. Conclusion: Vitamin E influenced the levels of neutrophils after physical exercise.Keywords: physical exercise, oxidative stress, vitamin E, neutrophil Abstrak: Latihan fisik merupakan aktivitas yang dilakukan seseorang untuk meningkatkan atau memelihara kebugaran tubuh. Latihan fisik dapat menimbulkan stres oksidatif sehingga dapat menurunkan aktivitas antioksidan. Vitamin E merupakan antioksidan yang larut dalam lemak dan berfungsi untuk mengurangi radikal bebas yang terdapat dalam tubuh. Neutrofil berperan aktif dalam proses fagositosis bakteri, mikroorganisme, dan membersihkan sisa jaringan rusak yang disebabkan oleh cedera jaringan. Penelitian ini bertujuan untuk mengetahui pengaruh vitamin E terhadap kadar neutrofil setelah latihan fisik. Jenis penelitian ialah eksperimental lapangan dengan pre post test control group design. Untuk menguji signifikansi penelitian digunakan uji Mann Whitney U (α = 0,05). Latihan fisik berupa olahraga futsal selama 60 menit dilakukan oleh 30 responden laki-laki yang dibagi dalam 2 kelompok yaitu perlakuan dan kontrol. Kadar neutrofil diukur setelah latihan fisik dan setelah pemberian vitamin E 400 IU selama 7 hari. Hasil statistik menunjukkan terdapat pengaruh bermakna dari vitamin E pada kelompok perlakuan (p=0,031). Simpulan: Terdapat pengaruh vitamin E terhadap kadar neutrofil setelah latihan fisik. Kata kunci: latihan fisik, stres oksidatif, vitamin E, neutrofil

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