scholarly journals Growth of Mutant Synechococcus SP. PCC 7002: Effects of Multi-Parameters and Prediction of Growth Rate

2021 ◽  
Vol 3 (1) ◽  
pp. 174-179
Author(s):  
Nor Fadhillah Mohamed Azmin ◽  
Atikah Mohamed Sharikh ◽  
Ummi S. Halmi Shari ◽  
Azlin Suhaida Azmi

Understanding of the correlative effects of combined variables on the growth rate of the cyanobacteria is fundamental to the exploitation of cyanobacteria as a biological mechanism to produce biofuels. Cyanobacteria (blue-green algae) are phototrophic microorganisms that offers attractive benefits, among which is a direct conversion of CO2 to a range of valuable products such as carbon-based biofuels. One model of cyanobacteria species is the cyanobacterium Synechococcus sp. PCC 7002. This paper describes the model developed to investigate the combined impacts of the variables on the growth of the Synechococcus sp. PCC 7002. The variables understudy include the temperature of the media, light intensity, the concentration of NaNO3, and the concentration of the NPK. The data is obtained from a lab scale study in which the Synechococcus sp. PCC 7002 underwent mutagenesis procedures. It is hypotheses that certain combination of the variables plays a key role in determining the growth rate of Synechococcus sp. 7002. The growth rate is determined through the measurement of four response variables, carbohydrate concentration, percentage of CO2 uptake, cell dry weight (CDW), and optical density (OD). A multivariate PCA model was developed which unearths the underlying relationship between the variables. Promising results were yield from the proposed model. Distinctive correlations between the variables were clearly described by the PCA model.

2002 ◽  
Vol 29 (3) ◽  
pp. 131 ◽  
Author(s):  
G. Dean Price ◽  
Shin-ichi Maeda ◽  
Tatsuo Omata ◽  
Murray R. Badger

Cyanobacteria (blue–green algae) have evolved a remarkable environmental adaptation for survival at limiting CO2 concentrations. The adaptation is known as a CO2 concentrating mechanism, and functions to actively transport and accumulate inorganic carbon (Ci; HCO3– and CO2) within the cell. Thereafter, this Ci pool is utilised to provide elevated CO2 concentrations around the primary CO2 fixing enzyme, Rubisco, which is encapsulated in a unique micro-compartment known as the carboxysome. Recently, significant progress has been gained in understanding the different types of Ci transport in cyanobacteria. This semi-review centres on the model cyanobacterium, Synechococcus sp. PCC7942, which possesses at least four distinct modes of Ci uptake when grown under Ci limitation, each possessing a high degree of functional redundancy. The four modes so far identified are: (i) BCT1, an inducible, high affinity HCO3– transporter of the bacterial ATP binding cassette transporter family, encoded by cmpABCD; (ii) a constitutive, Na+-dependent HCO3– transport system that can be allosterically activated (possibly by phosphorylation) in as little as 10 min; (iii) and (iv) two CO2 uptake systems, one constitutive and the other inducible, based on specialised forms of thylakoid-based, type 1, NAD(P)H dehydrogenase complexes (NDH-1). Here, we forward a speculative model that proposes that two unique proteins, ChpX and ChpY, possess CO2 hydration activity in the light, and when coupled to photosynthetic electron transport through the two specialised NDH-1 complexes, result in net hydration of CO2 to HCO3– as a crucial component of the CO2 uptake process.


2020 ◽  
Vol 15 (2) ◽  
pp. 233-237
Author(s):  
O A Lad ◽  
M M Kulkarni ◽  
S G Ragaji ◽  
M S Gavankar ◽  
M M Burondkar ◽  
...  

Konkan is considered as an important mango belt of India. This belt is not only famous for the production of the king of mango ‘Alphonso’ but also for the supply of quality planting material throughout the country. Soil is the basic medium used in the nursery. Availability of quality soil for nursery is getting scared and it is a need of the hour to find out lightweight, well-aerated media for reducing transport cost and mortality. Hence field experiment was carried out to find the response of mango cv. Alphonso stone grafts in the different potting mixtures. The treatment cocopeat + leaf manure + compost (1:1:2) was recorded significant increase in plant height (129.40%), girth of grafts (38.08%), highest number of shoot (1.50), number of leaf (22.70), highest absolute growth rate (0.1483 cm/day) and relative growth rate (0.0237 cm/cm/day). Whereas, maximum leaf area (617.03 cm2) was obtained in soil + leaf manure (1:1) followed by leaf manure + cocopeat (1:3) (610.17 cm2) leaf manure + cocopeat (1:3). Maximum root length (21.97 cm) and dry weight of root (7.23g) were obtained in treatment cocopeat + leaf manure + compost (1:1:1). The economics involved for different treatments showed that cocopeat + leaf manure + compost (1:1:2) was recorded with the highest B:C (1.39) followed by Soil + Cocopeat (1:1) in stone grafting. From the above investigation, it is concluded that potting mixture had a significant effect on the growth performance and economics of mango grafts. For raising mango grafts, the media containing cocopeat along with leaf manure and compost was the ideal soilless media.


1988 ◽  
Vol 68 (4) ◽  
pp. 935-940 ◽  
Author(s):  
M. TOLLENAAR ◽  
T. W. BRUULSEMA

The response of rate and duration of kernel dry matter accumulation to temperatures in the range 10–25 °C was studied for two maize (Zea mays L.) hybrids grown under controlled-environment conditions. Kernel growth rates during the period of linear kernel growth increased linearly with temperature (b = 0.3 mg kernel−1 d−1 °C−1). Kernel dry weight at physiological maturity varied little among temperature treatments because the increase in kernel growth rate with increase in temperature was associated with a decline in the duration of kernel growth proportional to the increase in kernel growth rate.Key words: Zea mays L, period of linear kernel dry matter accumulation, controlled-environment conditions, kernel growth rate


1927 ◽  
Vol 5 (1) ◽  
pp. 27-42
Author(s):  
JOSEPH NEEDHAM

1. The percentage growth-rate curves for carbohydrate and protein in the chick enbryo have been determined and compared with those already in the literature for the percentage growth-rates of the whole embryo, the dry substance, the fat, and the calorific value. 2. The composition of the embryo in gm. per cent, of dry weight has been calculated and an ontogenetic succession of carbohydrate, protein, and fat, has been brought to light. These three cell-constituents are at their highest levels in the embryo at 4, 11 and 20 days of incubation respectively. This may be correlated with the succession of maximum intensities of combustion, which have the same order (about 5,8.5 and about 18 days). 3. By comparing the curves for absorption-intensity with those representing the constitution of the embryo, it has been possible to trace the passage of building-materials from the yolk and white into the embryo and to analyse still further the movements of the fat and carbohydrate fractions. 4. The concentration of the carbohydrate and protein in the water of the embryonic body has been calculated and compared with a curve already in existence for fat. 5. The curve for intensity of absorption of total foodstuff has been calculated from the results of chemical analyses. It agrees as well as can be expected at present with that calculated from the oxygen consumption and the increase of dry substance in the embryo. 6. The ratio Carbohydrate : Protein decreases during development, thus falling into line with the ratios Water : Solid, Inorganic Substance : Organic Substance, and Protein : Fat. Certain speculations arising out of these facts are discussed. 7. There is a relation of simultaneity between the combustion of carbohydrate, protein, and fat, and the part these types of molecule play in the constitution of the whole embryo.


2021 ◽  
Vol 5 (2) ◽  
pp. 428-439
Author(s):  
K. M. Ladan ◽  
M. G. Abubakar ◽  
J. Suleiman

The Study was conducted to evaluate the effect of solid and liquid organic fertilizer on growth and yield of rosselle in 2016 cropping season at Institute of Agricultural Reserve Zaria, Samaru (11011’N 07038E and 686m) and Institute of Horticultural Research Farm Bagauda (12000’N 8031”Em 488m) in Northern Guinea Savannah and Sudan Savannah Ecological Zones of above sea level Nigeria. Treatments consisted of four levels of solid poultry manure (0.0, 1.0, 2.0 and 3.0) tons/ha and five levels of liquid organic manure from Grand Total Organic Fertilizer Limited (0.0, 0.5, 1.0, 1.5 and 2.9) litres/ha, which were factorially combined in a randomized complete block design (RCBD) and replicated three times. Data on growth parameter were collected on plant height (cm), plant dry weight (g), leaf Area index crop growth rate (CGR) Relative Growth Rate (RGR) and Net assimilation rate (NAR) while data on yield parameters like number of calyx per pant, 100 seed weight (g) and  calyx yield per hectare kg/ha were collected. Results showed that plant height, plant dry weight(g), 100 seed weight(g) and calyx dry yield kg/ha had a significant increase with application of 2.0 litres/ha of liquid fertilizer than other rates. While application of solid poultry manure at 3.0ton/ha significantly increases plant height, net assimilation rate, leaf area index and calyx dry weight when compared with other rates. From the results obtained, the combination of 2.0 litres/ha liquid organic fertilizer and 3.0 ton/ha solid poultry manure produce the highest calyx yield at both location.


2014 ◽  
Vol 2014 ◽  
pp. 1-13 ◽  
Author(s):  
Xibin Wang ◽  
Junhao Wen ◽  
Shafiq Alam ◽  
Xiang Gao ◽  
Zhuo Jiang ◽  
...  

Accurate forecast of the sales growth rate plays a decisive role in determining the amount of advertising investment. In this study, we present a preclassification and later regression based method optimized by improved particle swarm optimization (IPSO) for sales growth rate forecasting. We use support vector machine (SVM) as a classification model. The nonlinear relationship in sales growth rate forecasting is efficiently represented by SVM, while IPSO is optimizing the training parameters of SVM. IPSO addresses issues of traditional PSO, such as relapsing into local optimum, slow convergence speed, and low convergence precision in the later evolution. We performed two experiments; firstly, three classic benchmark functions are used to verify the validity of the IPSO algorithm against PSO. Having shown IPSO outperform PSO in convergence speed, precision, and escaping local optima, in our second experiment, we apply IPSO to the proposed model. The sales growth rate forecasting cases are used to testify the forecasting performance of proposed model. According to the requirements and industry knowledge, the sample data was first classified to obtain types of the test samples. Next, the values of the test samples were forecast using the SVM regression algorithm. The experimental results demonstrate that the proposed model has good forecasting performance.


2009 ◽  
pp. 131-139 ◽  
Author(s):  
Marija Skrinjar ◽  
Anamarija Mandic ◽  
Aleksandra Misan ◽  
Marijana Sakac ◽  
Ljubisa Saric ◽  
...  

An inhibitory effect of various concentrations (0.0, 0.5, 1.0, 1.5 and 2,0%) of mint (Mentha piperita L.) and caraway (Carvum carvi L.) on the growth of A. fumigatus, A. flavus and A. ochraceus was examined during 10 days of cultivation in YES medium at temperature of 25?C. Mint showed stronger inhibitory effect than caraway. Total dry weight (g/l) after 10 days of the growth of A. fumigatus in YES medium with 0.5% of mint decreased by about 95%, A. flavus by 97% and A. ochraceus by about 82%. Addition of higher concentrations of mint (1.0, 1.5 and 2.0%) reduced the growth of all tested species. It was poor and hardly visible. pH values of the media increased with the increase of mint concentrations. A. fumigatus showed the highest sensitivity towards caraway and A. flavus the lowest. Total dry weight (g/l) after 10 days of growth of A. fumigatus in medium with 0.5% of caraway decreased by about 72% in comparison to the control. In media with higher concentrations of caraway, its growth was found to be very poor. Concentration of 1.0% of caraway reduced A. flavus growth by 15% and of 1.5% by 92%, in regard to the control. In medium with 2.0% of caraway the growth of A. flavus was observed as poor and hardly visible. The growth of A. ochraceus in medium with 0.5% of caraway decreased by about 85% comparing with control and further decrease was noticed by the increase of concentrations. In medium with 1.5% of caraway a reduction of about 95% of growth was found and under 2.0% of caraway it was poor. pH of the media also increased with the increase of caraway concentrations. Applied concentrations of mint and caraway inhibited completely the production of AB1 by A. flavus.


2016 ◽  
Vol 1 (1) ◽  
pp. 013
Author(s):  
Aprilia Ike Nurmalasari ◽  
Eka Tarwaca Susila Putra ◽  
Prapto Yudono

The research aims to study the change of morphology root characters of eight hybrid oil palms under iron toxicity (Fe). Field experiment done in arranged in a Randomized Complete Block Design (RCBD) two factors and three blocks as replications. The first factor was Fe concentration. It consists of two levels which are concentration 0µ.g-1 and concentration 600 µg.g-1 Fe. The second factor is the hybrid of oil palms which consists of eight hybrid oil palms as Yangambi, Avros, Langkat, PPKS 239, Simalungun, PPKS 718, PPKS 540 and Dumpy. Fe was applied by pouring FeSO4 solvent for 600 µg.g-1 500 ml.-1plant.-1day-1 on two months of plants after transplanting in the main nursery. Data were collected on root morphology and plant dry weight The data were analysis of variance (ANOVA) at 5% significanly, followed by Duncan's multiple range test (DMRT). The relationships by among variables were determined by correlation analysis. The results showed that Fe concentration 600 µg.g-1 inhibits relatively root growth rate, narrows surface area, reduces the diameter, and shrinks root volume of all hybrid oil palms tested. The slowing relatively root growth rate, narrowing of root surface area and root diameter also root volume shrinkage due to Fe stress. It was also shown that the dry weight of plants was inhibit by existing of Fe toxicity.


2021 ◽  
Vol 2 ◽  
pp. 131-138
Author(s):  
Nandung Erlanda ◽  
Feira B. Arief ◽  
Ismahan Umran ◽  
Sutarman Gafur ◽  
Denah Suswati

Azotobacter nitrogen-fixing bacteria and urea fertilizer to reduce excessive use of chemical fertilizers. These bacteria can play a role in increasing nitrogen uptake and further growth of soybean plants. This study aimed to obtain growth media for Azotobacter and increase plant and root growth and nitrogen uptake of Biosoy soybean varieties. The research was conducted to help the uptake of n in the soil and reduce the excessive use of chemical fertilizers. The first experiment was carried out by growing bacteria on the media for 120 hours. The second experiment was designed in a completely randomized design that tested two treatments with additional use of urea fertilizer and Azotobacter and no Azotobacter. The results showed that there was no effect of giving Azotobacter from aloe vera gardens, and urea fertilizer showed a significant effect on the observed variables of soil pH in soybean plants on peat soil. In contrast, the variables observed were population, plant height, N content, N-total, plant dry weight, dry weight of plant roots, and N span of soybean plants had no significant effect.


Author(s):  
E. A. Effa ◽  
A. A. J. Mofunanya ◽  
B. A. Ngele

Background: Soil pH is one of the most important factors that contribute to crop growth and productivity. The present research was designed to assess the influence of soil amendment using organic manure and agricultural lime on the relative growth rate (RGR) and net assimilation rate (NAR) of Phaseolus vulgaris and Vigna aconitifolia grown on soils from different locations. Methodology: The three locations were: Akamkpa, Calabar Municipality and Odukpani. The pH for the three soil locations were 4.0, 7.0 and 9.0, respectively. The treatments were; control (0 g), OM1 (100 g organic manure), OM2 (200 g organic manure), AL1 (100 g agricultural lime), AL2 (200 g agricultural lime), OM1 + AL1 (50 g organic manure + 50 g agricultural lime) and OM2 +AL2 (100 g organic manure and 100 g agricultural lime). Results: Results obtained on the RGR of the leaf dry weight of P. vulgaris treated with OM2 was the highest (0.50 g/wk) followed by OM1 (0.41 g/wk). OM1 + AL1 had the highest RGR of the stem dry weight of P. vulgaris grown on soil from Calabar Municipality. In the RGR of the root dry weight, OM2 had the highest mean value in both plants grown on Akamkpa soil. Results obtained at 4 weeks after planting (WAP) revealed that there was significant (P<0.05) increase in NAR of plants grown on soil from Akamkpa. The highest NAR was obtained for V. aconitifolia treated with OM2 (0.0447 g/wk) followed by OM2 + AL2 (0.0057 g/wk) for both V. aconitifolia and P. vulgaris. P. vulgaris grown on Akamkpa and Odukpani soils treated with AL2 (0.0032 g/wk), OM1 + AL1 (0.0041 g/wk) and OM2+ AL2 (0.0062 g/wk) had the highest NAR at 8 WAP. Conclusion: The RGR and NAR of the two bean varieties were improved following treatments with organic manure and agricultural lime.


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