scholarly journals Phytochemicals Screening and Chromatographic Separation of Bio Active Compound from the Roots of Berberis Lyceum

2019 ◽  
pp. 1-5
Author(s):  
Shakir Ullah ◽  
◽  
Inam Ullah ◽  
Robeena Naz ◽  
◽  
...  
Keyword(s):  
Chromatographic Separation ◽  
Research Work ◽  
Ethanol Extract ◽  
Spectroscopic Methods ◽  
H Nmr ◽  
Phytochemical Investigation ◽  
Isolated Compound ◽  
First Time ◽  
Berberis Lycium

In the present research work the phytochemical and Chromatographic separation of bio active compounds Berberis lycium occurred. Phytochemical investigation of the CH2 Cl2 /CH3 OH (1:1) extract ethanol of root shown the presence of terpenoids, flavonoids, saponins, anthraquinones and alkaloids, steroids and tannins was absent. Chromatographic separation of CH2 Cl2 /CH3 OH (1:1) root ethanol extract yielded methyl 9, 10-dihydro-3, 8-hydroxy-6-methyl-9, 10-dioxoanthracene-2- carboxylate, isolated for the first time from the species of Berberis lycium. With the help of spectroscopic methods (IR, 1 H NMR, 13C NMR, and DEPT-135 and UV-Vis,) Partial characterization of the isolated compound was done.

scholarly journals New isoquinoline alkaloid from Carduus crispus L.

2014 ◽  
Vol 12 ◽  
pp. 85-87
Author(s):  
J Tunsag ◽  
G Davaakhuu ◽  
D Batsuren
Keyword(s):  
1H Nmr ◽  
Bioactive Compounds ◽  
13C Nmr ◽  
Ethanol Extract ◽  
Isoquinoline Alkaloid ◽  
Spectroscopic Methods ◽  
Aerial Parts ◽  
Isolated Compound ◽  
First Time

A new isoquinoline alkaloid, along with other known 4 bioactive compounds (1 isoquinoline alkaloid, 2 flavonoids, 1 coumarin), were isolated from the ethanol extract of aerial parts of Carduus crispus L. Crispine А N-oxide was newly isolated compound, two of them (quercetin and rutin) were compounds isolated for the first time from indicated plant. The structures of these compounds were elucidated and confirmed with spectroscopic methods e.g. 1H-NMR, 13C-NMR, EI-MS, COSY, HSQC, HMBC, and by comparison with literature data.DOI: http://dx.doi.org/10.5564/mjc.v12i0.178 Mongolian Journal of Chemistry Vol.12 2011: 85-87

Antioxidant Metabolites from Limonium brasiliense (Boiss.) Kuntze

2004 ◽  
Vol 59 (7-8) ◽  
pp. 477-480 ◽  
Author(s):  
Ana P. Murray ◽  
Silvana Rodriguez ◽  
María A. Frontera ◽  
María A. Tomas ◽  
María C. Mulet
Keyword(s):  
Chemical Constituents ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Spectroscopic Methods ◽  
H Nmr ◽  
Antioxidant Metabolites ◽  
First Time ◽  
C Nmr

Abstract A free radical scavenging activity guided fractionation of the polar extract from roots of Limonium brasiliense (Plumbaginaceae) led to the isolation of five active compounds including: myricetin 3-O-α-rhamnopyranoside (1), (-)-epigallocatechin 3-O-gallate (2), (-)-epigallocatechin (3), (+)-gallocatechin (4) and gallic acid (5). These and other chemical constituents are reported for the first time for this species. The characterization of these compounds was achieved by spectroscopic methods (1H NMR, 13C NMR and UV).

Chemical components of the rhizomes of Drynaria fortunei (KUNZE) J. Sm. (polypodiaceae) in Vietnam

2011 ◽  
Vol 76 (9) ◽  
pp. 1133-1139 ◽  
Author(s):  
Pham Thi Nhat Trinh ◽  
Nguyen Cong Hao ◽  
Phan Thanh Thao ◽  
Le Tien Dung
Keyword(s):  
Data Analysis ◽  
Spectral Data ◽  
Phenolic Acid ◽  
Ethanol Extract ◽  
Chemical Components ◽  
Spectroscopic Methods ◽  
Phenylpropanoid Glycoside ◽  
Spectral Data Analysis ◽  
First Time

From the ethanol extract of Drynaria fortunei (KUNZE) J. Sm., a new phenylpropanoid glycoside, fortunamide (1), was isolated and characterized by spectroscopic methods. Together with a new glycoside, 9 known compounds, including three curcuminoids (2–4), two isoprenylated flavonoids (5, 6), two flavonoids (7, 8), one monoterpenoid (9) and one phenolic acid (10) were isolated and identified by spectral data analysis from the rhizomes of Drynaria fortunei (KUNZE) J. Sm. Eight of them were isolated from Drynaria fortunei (KUNZE) J. Sm. for the first time.

Synthesis and characterization of meso-to-meso directly linked porphyrin-diazaporphyrin triads

2018 ◽  
Vol 22 (09n10) ◽  
pp. 814-820
Author(s):  
Yingying Jia ◽  
Ling Xu ◽  
Bangshao Yin ◽  
Mingbo Zhou ◽  
Jianxin Song
Keyword(s):  
Nickel Complex ◽  
High Resolution Mass Spectrometry ◽  
Dihedral Angles ◽  
X Ray Diffraction ◽  
Extinction Coefficients ◽  
X Ray ◽  
H Nmr ◽  
First Time ◽  
Molar Extinction Coefficients

Beginning with 5,10,15-triarylporphyrin-nickel complex, five meso-to-meso directly linked porphyrin-diazaporphyrin triads were successfully prepared for the first time through a series of reactions including formylation via Vilsmeier–Haack reaction, condensation with pyrrole, bromination with [Formula: see text]-Bromosuccinimide (NBS), oxidation with 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ), metal-templated cyclization of dibromodipyrrin-metal complexes with NaN[Formula: see text] and demetalization. All these triads were comprehensively characterized by [Formula: see text]H NMR, high-resolution mass spectrometry and UV-vis absorption. In addition, the structure of compound 6Ni was unambiguously determined by X-ray diffraction analysis, which showed that the two dihedral angles are both 86.65 (4)[Formula: see text] between each mean plane of porphyrin and that of central diazaporphyrin The UV-vis absorption spectra disclosed that the longest wavelengths of Soret bands and Q bands for these triads were observed at 429 and 642 nm, respectively. In contrast to diazaporphyrin-porphyrin dyads, diazaporphyrin dimers and diazaporphyrin monomers reported previously the molar extinction coefficients, particularly for triad 8Ni are much higher.

scholarly journals ISOLATION OF 2-CHLOROBENZIMIDAZOLE FROM MELIA DUBIA LEAF EXTRACT AND ITS STRUCTURAL CHARACTERISATION

2017 ◽  
Vol 9 (10) ◽  
pp. 67 ◽  
Author(s):  
G. Dayana Jeyaleela ◽  
S. Irudaya Monisha ◽  
J. Rosaline Vimala ◽  
A. Anitha Immaculate
Keyword(s):  
Mass Spectrometry ◽  
1H Nmr ◽  
Research Work ◽  
Proton Nuclear Magnetic Resonance ◽  
Resonance Spectroscopy ◽  
Ft Ir ◽  
Uv Visible ◽  
Isolated Compound ◽  
Promising Source

Objective: Natural products from medicinal plants, either as isolated compounds or as standardized plant extracts exhibit promising source of medicinal activity against various diseases. The aim of the present work was to make an attempt of isolation of bioactive principle and characterization of the isolated compound, from the medicinal plant Melia dubaiMethods: The extraction was done by a cold percolation method and the compound was separated and isolated by chromatography technique such as a thin layer chromatography (TLC), column chromatography and high-performance liquid chromatography (HPLC). The isolated compound was crystallized and the structural characterization of the isolated compound was made using UV-Visible, FT-IR, 1H-NMR, GC-MS and MS techniques which confirmed the structure of the isolated compound.Results: The separated and isolated compound was characterized by both physical and spectral methods like Ultraviolet-Visible spectroscopy (UV-Visible), Fourier transform infrared spectroscopy (FT-IR), Proton Nuclear Magnetic Resonance Spectroscopy (1H-NMR), Gas chromatography-mass spectrometry (GC-MS), and Mass spectrometry(MS). Based on the studies, organizational characteristics of one bioactive principle were deciphered. The results revealed that the isolated species is 2-chlorobenzimidazole and it agreed well with the reported value and spectra for 2-chlorobenzimidazole.Conclusion: The above results obtained in this research work clearly indicated the promising occurrence of 2-chlorobenzimidazole in Media dubia plant leaves. The future scope of these studies may guide us to view the biological activity of the isolated compound.

scholarly journals CONTRIBUTE TO STUDY ON CHEMICAL CONSTITUENTS OF URENA LOBATA (L.) OF VIETNAM

2019 ◽  
Vol 57 (2) ◽  
pp. 162
Author(s):  
Quan Minh Pham ◽  
Hoai Van Thi Tran ◽  
Lam Tien Do ◽  
Phuong Lan Doan ◽  
Inh Thi Cam ◽  
...  
Keyword(s):  
Mental Disorders ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Chemical Constituents ◽  
2D Nmr ◽  
Spectroscopic Methods ◽  
Tree Roots ◽  
Chemical Structures ◽  
Phytochemical Investigation ◽  
First Time

Urena lobata L. is used in Vietnamese traditional medicine for the treatment of several diseases. Tree roots are used to treat rheumatism, dysentery, poor digestion, flu, tonsils, malaria, asthma, goiter. Flowers are used to treat chickenpox, fever, and mental disorders. Branches, leaves or whole trees used to treat injuries bruises, rheumatism, mastitis, bites. Phytochemical investigation of the n-hexan and ethyl acetate extract of Urena lobata L. led to the isolation of β-sitosterol (1), β-sitosterol-3-O-β-D-glucopyranoside (2), a-acetylamino-phenylpropyl a-benzoylamino-phenylpropanoate (3), quercetin (4), and trans-tiliroside (5). Their chemical structures were determined by spectroscopic methods including MS, 1D, 2D NMR and comparing with those reported in previous papers. Two compounds 3, 5 were isolated for the first time from Urena lobata plant.

Isoflavones from Camphorosma lessingii Inhibit the Organic Anion Transporters OAT1 and OAT3

Planta Medica ◽  
2018 ◽  
Vol 85 (03) ◽  
pp. 225-230 ◽  
Author(s):  
Xinhui Wang ◽  
Dujuan Wang ◽  
Xue Wang ◽  
Manana Khutsishvili ◽  
Kamilla Tamanyan ◽  
...  
Keyword(s):  
Organic Anion ◽  
Inhibitory Effect ◽  
Spectroscopic Methods ◽  
Organic Anion Transporters ◽  
One Dimensional ◽  
Anion Transporters ◽  
Significant Inhibitory Effect ◽  
Phytochemical Investigation ◽  
The Family ◽  
First Time

AbstractPhytochemical investigation of Camphorosma lessingii has resulted in the isolation of four previously unreported isoflavones (1–4) and eight known compounds (5–12). Nine of these compounds (1–6, 8–10) are reported for the first time from members of the family Amaranthaceae. The structures of all isolated compounds were determined by spectroscopic methods, primarily one-dimensional and two-dimensional nuclear magnetic resonance and mass spectrometry. The absolute configuration of 6 was confirmed by circular dichroism. Inhibition of the organic anion transporters, OAT1 and OAT3, by the isolated compounds was evaluated. Among them, 7, 2′-dihydroxy- 6,8-dimethoxyisoflavone (1), 2′-hydroxy-6,7,8-trimethoxyisoflavone (2), 6,2′-dihydroxy-7,8-dimethoxyisoflavone (3), and 7-methoxyflavone (5) showed a significant inhibitory effect on 6-carboxyfluorescein uptake mediated by OAT1 and OAT3.

scholarly journals Structural and In Silico Characterization of Small Molecules Isolated from Eichhornia crassipes

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Andrew Mtewa ◽  
Duncan C. Sesaazi ◽  
Fanuel Lampiao
Keyword(s):  
In Silico ◽  
Eichhornia Crassipes ◽  
Cytochrome P450 Enzymes ◽  
Anticancer Activities ◽  
H Nmr ◽  
Cytotoxic Compounds ◽  
In Silico Characterization ◽  
First Time ◽  
Nonanedioic Acid

Eichhornia crassipes has been reported to have various medicinal properties including anticancer activities. The plant was collected from the Shire river in Malawi, and two cytotoxic compounds, benzene-1,4-diol and nonanedioic acid, were isolated and characterized for the first time in the leaves and roots of the plant. 1H NMR, COSY, HSQC, HMBC, 13-C, and LCMS spectroscopic experimental techniques were used to identify the compounds in their pure forms. In silico experiments showed that both compounds do not have AMES toxicity and do not inhibit cytochrome P450 enzymes, but nonanedioic acid acts as a CYP2D6 substrate. This work showed that Eichhornia crassipes can be considered to have a role as a source of potential hits and leads to drug development that can be rationally optimized for drugs.

scholarly journals Phytochemical Characterization of an Adaptogenic Preparation from Rhodiola heterodonta

2009 ◽  
Vol 4 (8) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Mary H. Grace ◽  
Gad G. Yousef ◽  
Anvar G. Kurmukov ◽  
Ilya Raskin ◽  
Mary Ann Lila
Keyword(s):  
Methyl Ether ◽  
Epigallocatechin Gallate ◽  
Ethanol Extract ◽  
Biologically Active ◽  
Etoh Extract ◽  
Phytochemical Constituents ◽  
First Time ◽  
Adaptogenic Activity ◽  
Oligomeric Compounds

The phytochemical constituents of a biologically active, standardized, 80% ethanol extract of Rhodiola heterodonta were characterized. The extract was fractionated over a Sephadex LH-20 column to afford two main fractions representing two classes of secondary metabolites: phenylethanoids and proanthocyanidins. This fractionation facilitated the identification and quantification of individual compounds in the fractions and sub-fractions using HPLC, and LC-MS. The major compounds in the phenylethanoid fraction were heterodontoside, tyrosol methyl ether, salidroside, viridoside, mongrhoside, tyrosol, and the cyanogenic glucoside rhodiocyanoside A. These seven compounds comprised 17.4% of the EtOH extract. Proanthocyanidins ranged from oligomers to polymers based on epigallocatechin and gallate units. The main identified oligomeric compounds in the proanthocyanidin fraction were epigallocatechin gallate, epigallocatechin-epigallocatechin-3-O-gallate and 3-O-galloyl-epigallocatechin-epigallocatechin-3-O-gallate, which constituted 1.75% of the ethanol extract. Tyrosol methyl ether, mongrhoside, and the two proanthocyanidin dimers were reported for the first time from this species in this study. Intra-peritoneal injection of the 80% ethanol extract increased survival time of mice under hypoxia by 192%, as an indication of adaptogenic activity.

scholarly journals Bioactive Components of Fissistigma cupreonitens

2018 ◽  
Vol 13 (6) ◽  
pp. 1934578X1801300 ◽  
Author(s):  
I-Hsiao Chen ◽  
Ming-Yi Yang ◽  
Shin-Hun Juang ◽  
Chia-Lin Lee ◽  
Tran-Dinh Thang ◽  
...  
Keyword(s):  
Cell Line ◽  
Human Cancer ◽  
Spectroscopic Methods ◽  
Human Cancer Cell ◽  
Bioactive Components ◽  
Standard Drug ◽  
Human Cancer Cell Lines ◽  
Chemical Structures ◽  
Phytochemical Investigation ◽  
First Time

Phytochemical investigation of Fissistigma cupreonitens (Annonaceae) led to the isolation of 34 compounds. The chemical structures of all compounds were determined by spectroscopic methods. Among the isolates, compounds 20–27 and 31–34 were reported from this genus for the first time. From the results of the cytotoxicity assay against three human cancer cell lines (NCI-H226, NPC-TW01, and Jurkat E6–1), oxoaporphine compounds oxoxylopine (1), oxocrebanine (3), kuafumine (4) and lysicamine (5), and the flavonoid adunctin E (26) displayed significant cytotoxicity against NCI-H226 cell line, with IC50 values of 8.45, 8.10, 8.54, 12.83 and 12.00 μM, respectively, in comparison with the standard drug, cisplatin with IC50 of 13.37 μM.

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