scholarly journals Comparative analysis of the repellency activity of single compounds and the synthetic blends of semiochemicals from Aframomum melegueta (R.schum) and Dennittia tripetala (Bak.F) against Rhizopertha dominica in Calabar, Nigeria

2021 ◽  
Vol 1 (2) ◽  
pp. 59-69
Author(s):  
Lawrence Ugbe
Keyword(s):  
Essential Oils ◽  
Insect Pests ◽  
Chemical Constituents ◽  
Insect Pest ◽  
Research Work ◽  
Reproductive Potential ◽  
Sub Saharan Africa ◽  
Small Scale ◽  
Rhizopertha Dominica ◽  
Repellency Activity

Bioassay experiments were carried out in Calabar to compare the repellency activity of single compounds and the synthetic blends of Semiochemicals extracted from Afromomum melegueta and Dennittia tripetala. The aim of the experiment was to determine the efficacy of single compounds and the synthetic blends in suppressing the reproductive potential and adult emergence of Rhizopertha dominica, (Fabricius), a primary pest of stored cassava pellets and cereal grains in Sub-Saharan Africa. Insect culture of the adult R. dominica was set up in the laboratory at Federal College of Education Obudu to obtain fresh insects for the bioassay experiments. Dried seeds of A. melegueta (R. Schum) and D. tripetala (Baker f.) Schatz were procured from the main market in Obudu for the purpose of the research work. 100g each of the dried fruits of the spice plants were pounded separately with the laboratory pestle and mortar for the extraction of the essential oils (EOs). The oils extracted were tested for toxicity and reproductive potential deterrence against R. dominica. The chemical constituents of the essential oils were isolated, identified and tested against R. dominica. Synthetic blends of individual compounds were prepared based on their natural ratios and were also tested for toxicity and reproductive potential deterrence against the insect pests in the laboratory. The result showed that both the single compounds and the synthetic blends were toxic and repellent to the insect pest, and could significantly suppress the reproductive potential thereby inhibiting the emergence of adult insect. However, synthetic blends exhibited a stronger efficacy in their toxicity against the pest thereby providing a broad spectrum of insecticidal bioactivity against R. dominica than the single compounds. This action of the synthetic blends of extracts from the spice plants demonstrated their potential for development in stored products protection especially at the small scale resource poor farmer’s level in Nigeria.

scholarly journals The Pathogenicity of Beauveria bassiana on Diatraea centrella Larva of Sugarcane in Guyana

2019 ◽  
Vol 7 (1) ◽  
Author(s):  
Basantie Sukhu ◽  
Gomathinayagam Subramanian ◽  
Abdullah Ansari
Keyword(s):  
Biological Control ◽  
Organic Farming ◽  
Beauveria Bassiana ◽  
Insect Pests ◽  
Insect Pest ◽  
Research Work ◽  
Control Agent ◽  
Small Scale ◽  
Pests And Diseases ◽  
Short Period

Sugarcane is a highly beneficial crop grown worldwide and is greatly influenced by numerous insect pests. These insect pests have adapted to the diversity of conditions under which sugarcane is grown thus causing a great degree of damage to the crop. These insect pests feed on different parts of the sugarcane. For instance, the early shoot borer attacks the cane before internode formation, while the internode borer feeds after internode formation, the foliar pest feeds on the leaves and some would feed on the rootstalks.Guyana has large areas of land which are free of agricultural chemicals and thus are suitable for organic farming. Organic farming is practiced in Guyana on a small scale. However, crops such as cocoa, pineapple and heart of palm have already been grown organically for export. Similarly, like organic farming, biological control of pests and diseases is also encouraged worldwide. Biological control in agriculture is a method of controlling pests such as insects, weeds and diseases by using natural enemies such as predators, parasites, herbivores, or any other natural mechanisms. In addition, like organic farming biological controls are also ecologically friendly and are very effective. Both approaches (organic farming and biological control) are significant since the use of pesticides and chemical fertilizers would be eliminated, thus reducing health issues in the environment and of consumers.                This research work is on the pathogenicity of Beauveria bassiana on the insect pest Diatraea centrella larva of sugarcane in Guyana. The entomopathogenic fungus, Beauveria bassiana, is a promising control agent against many insect pests [1]. Beauveria bassiana is commonly found in soils worldwide and has a host range of over 700 insect species, including insects from the orders Lepidoptera, Coleoptera and Hymenoptera.  The research carried out focuses on the effect of Beauveria bassiana spores on Diatraea centrella larva and the rate at which these spores are effective, as well as the target site of infection of these spores on the larva.               The study conclusively proves that a higher number of Beauveria bassiana spores are more effective on Diatraea centrella larva in a short period of time and the dorsal, ventral and intestinal tissues of the Diatraea centrella larva are more susceptible to the spores of Beauveria bassiana. 

scholarly journals An evaluation of the efficacy of extracts from Xylopia aethiopica and Dennittia tripetala in controlling Dermestes maculatus(degeer) an insect pest of dry stored fish in Calabar, Nigeria

2021 ◽  
Vol 10 (1) ◽  
pp. 1-6
Author(s):  
Fidelis Bekeh Ada
Keyword(s):  
Essential Oils ◽  
Adult Population ◽  
Insect Pest ◽  
Chemical Constituent ◽  
College Of Education ◽  
Sub Saharan Africa ◽  
Small Scale ◽  
Local Market ◽  
Sub Saharan ◽  
Xylopia Aethiopica

Laboratory bioassays were conducted in Obudu, Nigeria to evaluate the efficacy of extracts from X.aethiopica and D.tripetala in controlling D.maculatus a primary pest of dry stored fish in sub-saharan Africa. Adult population of D. maculatus was obtained in the laboratory at the Federal college of Education Obudu, to obtain fresh insects for the experimental work. Dried fruits of X.aethiopica (Dunal) and dried seeds of D.tripetala (Bak.f.) were procured from a local market in Obudu for the purpose of the work. 100g each of the spice plants were pounded separately using laboratory pestle and mortar for the extraction of essential oils (EOS). The essential oils extracted were tested for repellence and toxicity against D.maculatus at 10µl per EO in a 4-way Olfactometer. Minitab 15 statistical soft ware was used for the analysis of data. Results indicated that the essential oils (EOS) and their constituent individual compounds were significantly (p<0.05) repellent and toxic to D.maculatus with X.aethiopica more repellent and more toxic to the insect than D.tripelala. The two oils were also observed to inhibit the activities of microbes that usually cause and accelerate delaying in stored fish. This action of the essential oils of the two spice plants and their chemical constituent demonstrated their potentials for development in stored products protection, especially at the small scale resource poor farmer’s level in Nigeria.

scholarly journals Essential Oils as Post-Harvest Crop Protectants against the Fruit Fly Drosophila suzukii: Bioactivity and Organoleptic Profile

Insects ◽  
2020 ◽  
Vol 11 (8) ◽  
pp. 508 ◽  
Author(s):  
Stefano Bedini ◽  
Francesca Cosci ◽  
Camilla Tani ◽  
Erika Carla Pierattini ◽  
Francesca Venturi ◽  
...  
Keyword(s):  
Essential Oils ◽  
Chemical Constituents ◽  
Insect Pest ◽  
Fruit Fly ◽  
Drosophila Suzukii ◽  
Negative Effects ◽  
Tea Tree ◽  
Post Harvest ◽  
Organoleptic Characteristics ◽  
Low Concentrations

The essential oils extracted from mandarin (Citrus reticulata Blanco) fruits, and from tea tree (Maleleuca alternifolia (Maiden and Betche) Cheel) leaves have been chemically analyzed and tested for their bioactivity against D. suzukii. Besides, to estimate consumers’ acceptability of the essential oil (EO) treatments, we evaluated their impact on the organoleptic characteristics of the EO-treated fruits. The main chemical constituents of the two EOs were 1,8-cineole and 4-terpineol for M. alternifolia (22.4% and 17.6% of the total components, respectively), and limonene (83.6% of the total components) for C. reticulata. The behavioral tests indicate that the two EOs are able to deter D. suzukii oviposition and that D. suzukii shows positive chemotaxis to low concentrations of the EOs and negative chemotaxis when the EO concentration increases. While no negative effects on the organoleptic profiles were detected for fruits treated with C. reticulata EO, the olfactory profile of fruits treated with M. alternifolia EO was so negative that they were defined as “not suitable for consumption” by panellists. Overall, our findings indicate that the use of EOs for the post-harvest protection of small fruits is feasible, provided that the essential oils are selected not only for their bioactivity against the insect pest but also for their affinity with the consumers’ sensorial system.

Ovicidal, larvicidal, and behavioural effects of some plant essential oils on diamondback moth (Lepidoptera: Plutellidae)

2017 ◽  
Vol 149 (5) ◽  
pp. 639-648 ◽  
Author(s):  
Jatinder S. Sangha ◽  
Tess Astatkie ◽  
G. Christopher Cutler
Keyword(s):  
Essential Oils ◽  
Insect Pests ◽  
Low Cost ◽  
Insect Pest ◽  
Diamondback Moth ◽  
Abies Balsamea ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Larval Feeding ◽  
Plant Essential Oils

AbstractAlternatives to synthetic insecticides are desirable for management of diamondback moth, Plutella xylostella (Linnaeus) (Lepidoptera: Plutellidae), an insect pest of global importance. Many essential oils derived from aromatic plants have demonstrated toxicity and behaviour altering effects on insect pests, and are considered low-risk alternatives to synthetic insecticides. We conducted laboratory experiments to determine the biological activity of several low-cost, commercially available essential oils against P. xylostella. Experiments testing ovicidal effects, larvicidal effects, larval feeding deterrence, and adult oviposition deterrence were done with essential oils derived from Artemisia abrotanum Linnaeus (Asteraceae), balsam fir (Abies balsamea Linnaeus (Pinaceae)), black pepper (Piper nigrum Linnaeus (Piperaceae)), eucalyptus (Eucalyptus polybractea (Baker) (Myrtaceae)), garlic (Allium sativum Linnaeus (Amaryllidaceae)), rosewood (a blend of different oil constituents), tansy (Tanacetum vulgare Linnaeus (Asteraceae)), and thyme (Thymus zygis Linnaeus (Lamiaceae)), using concentrations of 1, 2.5, and 5% v/v. Although all essential oils had some level of bioactivity against certain P. xylostella life stages, essential oils from garlic, rosewood, and thyme were most effective overall, demonstrating significant ovicidal and larvicidal activity, as well as deterrent effects on larval feeding and settling behaviour, and adult oviposition. Although variable phytotoxicity was observed with essential oils at 2.5% and 5% v/v concentrations, the results suggest that rosewood, garlic, and thyme essential oils have potential in management of P. xylostella.

scholarly journals Evaluation of the chemical composition and bioactivity of essential oils from Cinnamomum camphora (L.) J.Presl. (Lauraceae), Piper nigrum L. (Piperaceae) and Stemona japonica (Miq.) (Stemonaceae) against Oryzaephilus surinamensis (Linnaeus, 1758) (Coleoptera: Silvanidae)

2019 ◽  
Vol 88 (3) ◽  
pp. 199-213
Author(s):  
Tufail Ahmed Wagan ◽  
Xiang Li ◽  
Hongxia Hua ◽  
Wanlun Cai
Keyword(s):  
Essential Oils ◽  
Filter Paper ◽  
Insect Pests ◽  
Insect Pest ◽  
Gas Chromatography Mass Spectrometry ◽  
Piper Nigrum ◽  
Cinnamomum Camphora ◽  
Oryzaephilus Surinamensis ◽  
Filter Paper Disc ◽  
Males And Females

Abstract Oryzaephilus surinamensis is a serious insect pest of many stored products. Repellency and fumigation tests were performed to manage it. Essential oils (EO) from three plants – Cinnamomum camphora, Piper nigrum and Stemona japonica – were tested on Oryzaephilus surinamensis in the laboratory. Repellency was tested on filter paper in Petri dishes at a concentration of 31.4 μg/cm2, and 20 adults (10 days old) were observed for repellency behaviour 12, 24, 48 and 96 hours after the bioassay without any food. Cinnamomum camphora EO was the strongest repellent (83.75 ± 1.83%), followed by the Piper nigrum and Stemona japonica EOs. The eggs were obtained by maintaining 20 individuals (males and females) for 24 h in a 100 mL glass jar containing 5 g of diet feed. To evaluate the effect of EO on the development of larvae and adults, a filter paper disc impregnated with 0.1 mL of EO was placed on the inner side of the lid of the jar containing the eggs and diet feed. After 40 days, the number of adults and larvae in the jar was counted. All three EOs exhibited bioactivity against the development of larvae and adults. A relatively low number of larvae and adults was recorded in Cinnamomum camphora, followed by Stemona japonica and Piper nigrum. Gas chromatography/mass spectrometry was used to identify the major compounds in the EOs. Further studies are recommended to evaluate their bioactivity against different insect pests in stored seeds.

​Effects of Genotypic Diversity on Insect-pests of Soybean

2021 ◽  
Author(s):  
Lokesh Kumar Meena ◽  
Vangala Rajesh ◽  
Amar Nath Sharma
Keyword(s):  
Effective Treatment ◽  
Natural Enemy ◽  
Agronomic Traits ◽  
Insect Pests ◽  
Block Design ◽  
Insect Pest ◽  
Genotypic Diversity ◽  
Intraspecific Diversity ◽  
Small Scale ◽  
Randomized Block Design

Background: Intercropping and mixed cropping based on two or more different crops have been widely used in Insect Pest Management to manage the population density of insect pests in various crops like maize with soybean, sorghum with soybean and sunflower with soybean. Genotypic diversity is the mixture or combination of various varieties/lines/genotypes of a crop. It decreases the insect-pests population by unfitness of crop to them due to intraspecific diversity such as mixture of susceptible and resistant varieties. Also, it increases the natural enemy population more due to longer period of flowering, availability of wide diverse food, more movement of herbivores etc. This concept has also been used successfully to manage insect-pests in maize, wheat and potato. In India, this connect is used in very small scale in crop. The objective of this study was to determine effects of genotypic diversity by comparing the population densities of major insect pests between monoculture of single variety and mixture of varieties. Methods: To test the effects of genotypic diversity on soybean insect-pest and their natural enemy population an experiment was laid in kharif 2018 and 2019 at the ICAR-Indian Institute of Soybean Research, Indore. All recommended agronomic package of practices were followed for raising of crop. Randomized block design with three replication having plot size of 9 rows of 5 meter row length was used. Totally 20 treatments were chosen for this experiment and out of 20 four treatments namely, T1, T6, T11 and T16 were the treatments of four varietal mixture treatments of almost similar maturity duration and agronomic traits and rest of treatments were of sole varietal treatments. Observations were made on insect-pests from 10 selected plants in each plot on three leaves selected at top, middle and lower portion of plant. Result: Effects of genotypic diversity in soybean revealed that for whitefly management T11 (NRC-86, JS-335, JS-2098 and RKS-45) was found most effective treatment. In case of mite, thrips and treehoppers management T1 (JS-9560, JS-20-34, MAUS-47 and MAUS-1460) was found most effective treatment. For leafhopper and mealy bug T6 (JS-9305, JS-2029, RVS-2001-4 and Dsb-28-3) was found most effective treatment. While for spittlebug T16 (NRC-37, JS-9752, RSC-1046 and RKS-113) was found most effective treatment. So, genotypic diverse treatments were found more effective than their respective sole varietal treatments.

scholarly journals Qualitative detection of different enzymes from the gut and salivary glands of Nezara viridula Linn. using the chemical inference

2020 ◽  
Vol 5 (4) ◽  
pp. 530-534
Author(s):  
Jitendra Rajak
Keyword(s):  
Salivary Glands ◽  
Feeding Behaviour ◽  
Large Scale ◽  
Insect Pests ◽  
Insect Pest ◽  
Research Work ◽  
Crop Productivity ◽  
Nezara Viridula ◽  
Stink Bug ◽  
Southern Green Stink Bug

Globally the crop insect pests damage the 30% of agriculture production due to insect spoil, and the situation is very awful in particularly developing countries such as India.  There are large number of insect pest families are well known which induce the critical destroy to agriculture crops. Crop insect pest’s harm is one of the significant features that ruin the agriculture crop productivity, although the large-scale utilization of chemical pesticides. The successfulness of the pesticides depends upon the interaction of chemicals with the physiology of the insect of the pests. In India, the Nezara viridula Linn, is one of the critical insects because of its destructive feeding on major crop which is the utmost source of economic deprivation in agriculture. The enzymes present in the gut and salivary glands of the N. Viridula play key role in the feeding behaviour. Presently very little is investigated about the gut and salivary glands of the N. Viridula at minute level. We first time investigated the different enzymes existing in the gut and salivary gland using the chemical inference. This research work is carried out to analysis the enzymes for digestive and feeding behaviour of N. Viridula which will assist the biologists to manage the challenges are created by their resistance to the traditional chemical insecticides used for prevention of N. Viridula (a type of southern green stink bug) inhabitants and high reproductive amplitude. The extensive statistics of invertase enzymes, maltase, alpha galactosidase, amylase, esterase, proteinase and polypeptidase enzymes for the N. Viridula in salivary glands and gut inspired by this research imparts supplementary resource for further understanding of the biology of this Pentatomidae (Heteroptera) species.

Insect Pest Management in Stored Products

2020 ◽  
Vol 31 (1) ◽  
pp. 24-35 ◽  
Author(s):  
Somiahnadar Rajendran
Keyword(s):  
Pest Management ◽  
Insect Pests ◽  
Insect Pest ◽  
Control Measures ◽  
Stored Products ◽  
Insect Pest Management ◽  
Insect Infestation ◽  
Combination Treatments ◽  
Agricultural Produce ◽  
Food Commodities

Insects are a common problem in stored produce. The author describes the extent of the problem and approaches to countering it. Stored products of agricultural and animal origin, whether edible or non-edible, are favourite food for insect pests. Durable agricultural produce comprising dry raw and processed commodities and perishables (fresh produce) are vulnerable to insect pests at various stages from production till end-use. Similarly, different animal products and museum objects are infested mainly by dermestids. Insect pests proliferate due to favourable storage conditions, temperature and humidity and availability of food in abundance. In addition to their presence in food commodities, insects occur in storages (warehouses, silos) and processing facilities (flour mills, feed mills). Insect infestation is also a serious issue in processed products and packed commodities. The extent of loss in stored products due to insects varies between countries depending on favourable climatic conditions, and pest control measures adopted. In stored food commodities, insect infestation causes loss in quantity, changes in nutritional quality, altered chemical composition, off-odours, changes in end-use products, dissemination of toxigenic microorganisms and associated health implications. The insects contribute to contaminants such as silk threads, body fragments, hastisetae, excreta and chemical secretions. Insect activity in stored products increases the moisture content favouring the growth of moulds that produce mycotoxins (e.g., aflatoxin in stored peanuts). Hide beetle, Dermestes maculatus infesting silkworm cocoons has been reported to act as a carrier of microsporidian parasite Nosema bombycis that causes pebrine disease in silkworms. In dried fish, insect infestation leads to higher bacterial count and uric acid levels. Insects cause damage in hides and skins affecting their subsequent use for making leather products. The trend in stored product insect pest management is skewing in favour of pest prevention, monitoring, housekeeping and finally control. Hermetic storage system can be supplemented with CO2 or phosphine application to achieve quicker results. Pest detection and monitoring has gained significance as an important tool in insect pest management. Pheromone traps originally intended for detection of infestations have been advanced as a mating disruption device ensuing pest suppression in storage premises and processing facilities; pheromones also have to undergo registration protocols similar to conventional insecticides in some countries. Control measures involve reduced chemical pesticide use and more non-chemical inputs such as heat, cold/freezing and desiccants. Furthermore, there is an expanding organic market where physical and biological agents play a key role. The management options for insect control depend on the necessity or severity of pest incidence. Generally, nonchemical treatments, except heat, require more treatment time or investment in expensive equipment or fail to achieve 100% insect mortality. Despite insect resistance, environmental issues and residue problems, chemical control is inevitable and continues to be the most effective and rapid control method. There are limited options with respect to alternative fumigants and the alternatives have constraints as regards environmental and health concerns, cost, and other logistics. For fumigation of fresh agricultural produce, new formulations of ethyl formate and phosphine are commercially applied replacing methyl bromide. Resistance management is now another component of stored product pest management. In recent times, fumigation techniques have improved taking into consideration possible insect resistance. Insect control deploying nanoparticles, alone or as carriers for other control agents, is an emerging area with promising results. As there is no single compound with all the desired qualities, a necessity has arisen to adopt multiple approaches. Cocktail applications or combination treatments (IGRs plus organophosphorus insecticides, diatomaceous earth plus contact insecticides, nanoparticles plus insecticides/pathogens/phytocompounds and conventional fumigants plus CO2; vacuum plus fumigant) have been proved to be more effective. The future of store product insect pest management is deployment of multiple approaches and/or combination treatments to achieve the goal quickly and effectively.

Life Cycle Development of Successful Women-owned Enterprises

2020 ◽  
Vol 3 (4) ◽  
pp. 142-152
Author(s):  
Mohammad Waliul Hasanat ◽  
Kamna Anum ◽  
Ashikul Hoque ◽  
Mahmud Hamid ◽  
Sandy Francis Peris ◽  
...  
Keyword(s):  
Life Cycle ◽  
Data Collection ◽  
Research Work ◽  
Primary Data ◽  
Small Scale ◽  
Data Collection Process ◽  
Theoretical Finding ◽  
Successful Women ◽  
Two Phases ◽  
Life Cycle Development

In developing countries, the role of women in the business sector is continuously improving. As a result, female enterprises have also been encouraged in Pakistan. This study is based on life cycle development phases from which women-owned enterprises have to go through in order to become successful. As a primary data source, face-to-face interviews with owners of successful women-owned enterprises were preferred. The data collection process was divided into two phases i.e. Phase-I and Phase-II. After data collection, qualitative analysis has been performed using NVIVO. Findings provide both generic and specific factors involved in life cycle development of women-owned enterprises. This study provides a detailed view of life cycle development model followed by successful women enterprises. The outcome of this research work is a theoretical finding which can be utilized by entrepreneurs owning small scale enterprises to improve their level of performance. Findings can also be helpful for potentially talented women interested in setting up their own business.

Chemical Constituents and Antibacterial Activity of Cissus Aralioides.

2020 ◽  
Vol 17 ◽  
Author(s):  
Balogun Olaoye Solomon ◽  
Ajayi Olukayode Solomon ◽  
Owolabi Temitayo Abidemi ◽  
Oladimeji Abdulkarbir Oladele ◽  
Liu Zhiqiang
Keyword(s):  
Plant Extract ◽  
Chemical Constituents ◽  
Ethyl Acetate Fraction ◽  
Antibacterial Activities ◽  
Sub Saharan Africa ◽  
Chromatographic Purification ◽  
Whole Plant ◽  
Sub Saharan ◽  
First Time

: Cissus aralioides is a medicinal plant used in sub-Saharan Africa for treatment of infectious diseases; however the chemical constituents of the plant have not been investigated. Thus, in this study, attempt was made at identifying predominant phytochemical constituents of the plant through chromatographic purification and silylation of the plant extract, and subsequent characterization using spectroscopic and GC-MS techniques. The minimum inhibitory concentration (MICs) for the antibacterial activities of the plant extract, chromatographic fractions and isolated compounds were also examined. Chromatographic purification of the ethyl acetate fraction from the whole plant afforded three compounds: β-sitosterol (1), stigmasterol (2) and friedelin (3). The phytosterols (1 and 2) were obtained together as a mixture. The GC-MS analysis of silylated extract indicated alcohols, fatty acids and sugars as predominant classes, with composition of 24.62, 36.90 and 26.52% respectively. Results of MICs indicated that friedelin and other chromatographic fractions had values (0.0626-1.0 mg/mL) comparable with the standard antibiotics used. Characterization of natural products from C. aralioides is being reported for the first time in this study.

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