scholarly journals Natural fruit peels as teeth whitening agents on primary teeth an in vitro study

2021 ◽  
Vol 12 (2) ◽  
pp. 232-235
Author(s):  
Archana P Betur ◽  
Raju O S ◽  
Roopa K B
Keyword(s):  
Orange Peel ◽  
Study Groups ◽  
Enamel Surface ◽  
Banana Peel ◽  
Staining Procedure ◽  
Control Group ◽  
Standard Group ◽  
Deciduous Dentition ◽  
Anterior Teeth

Context: Dental Bleaching or tooth whitening represents a conservative cosmetic approach even for children and adolescents, for whom treatment may be readily accomplished without permanent alteration to the enamel surface thus limiting the need for lifelong maintenance. Aims: The aim of this study was to compare the efficacy of banana peel and orange peel as teeth   whitening agents in deciduous dentition. Methods and Material: Twenty extracted primary anterior teeth were used in this study and each crown was sectioned mesiodistally to obtain 40 samples which were flattened to create a standardized enamel surface. After the staining procedure, 40 samples were allocated into 4 groups, i.e., 10 samples per group, the standard group (35% Hydrogen peroxide), the control group (distilled water), banana peel and orange peel. The samples of each group were treated using the respective agents with standardized pressure and time. Results: The tooth shades were assessed using Digital Spectrophotometer at baseline, 1 week of staining, 1 week of bleaching and 1 week post bleaching. All the results were statistically analysed with Post Hoc Tukey’s test. Both the study groups showed significant changes but, banana peel showed better efficacy than orange peel. Conclusions: Banana peel and orange peel can be considered for teeth whitening in deciduous dentition.

Treatment with roscovitine and butyrolactone I prior to in vitro maturation alters blastocyst production

Zygote ◽  
2019 ◽  
Vol 28 (1) ◽  
pp. 24-31 ◽  
Author(s):  
Rosiara Rosária Dias Maziero ◽  
Carlos Renato de Freitas Guaitolini ◽  
Daniela Martins Paschoal ◽  
André Maciel Crespilho ◽  
Bianca Andriolo Monteiro ◽  
...  
Keyword(s):  
In Vitro Maturation ◽  
Study Groups ◽  
Embryo Cryopreservation ◽  
Control Group ◽  
Bovine Embryos ◽  
Nuclear Maturation ◽  
Oocyte Meiosis ◽  
Maturation Index ◽  
Bovine Oocytes

SummaryThis study evaluated the effects of oocyte meiosis inhibitors roscovitine (ROS) and butyrolactone I (BL-I) on in vitro production of bovine embryos. Bovine oocytes were maintained in pre in vitro maturation (pre-IVM) with 25 µM ROS or 100 µM BL-I for 24 h to delay meiosis and for 24 h in in vitro maturation (IVM). Following this treatment, the nuclear maturation index was evaluated. All embryos degenerated following this procedure. In the second set of experiments, oocytes were maintained for 6 or 12 h in pre-IVM with the following three treatments: ROS (25 µM or 12.5 µM), BL-I (100 µM or 50 µM) or a combination of both drugs (6.25 µM ROS and 12.5 µM BL-I). Oocytes were cultivated for 18 or 12 h in IVM. When a meiosis-inducing agent was used during pre-IVM for 24 h, more degenerated oocytes were observed at the end of the IVM period. This effect decreased when the meiotic blocking period was reduced to 6 or 12 h. No significant differences were observed in the blastocyst production rate of oocytes in pre-IVM for 6 h with ROS, BL-I, or ROS + BL-I compared with that of the control group (P > 0.05). However, inhibition of oocytes for 12 h resulted in decreased embryo production compared with that in the controls (P < 0.05). There was no difference in the post-vitrification embryo re-expansion rate between the study groups, showing that the meiotic inhibition for 6 or 12 h did not alter the embryo cryopreservation process.

scholarly journals The Influence of Post Bleaching Treatments in Stain Absorption and Microhardness

2016 ◽  
Vol 10 (1) ◽  
pp. 69-78 ◽  
Author(s):  
Horieh Moosavi ◽  
Fatemeh Darvishzadeh
Keyword(s):  
Color Change ◽  
Enamel Surface ◽  
Control Group ◽  
Surface Microhardness ◽  
Dental Bleaching ◽  
Tooth Color ◽  
Group 4 ◽  
Group 3 ◽  
Group 2

Objectives: This study investigated the effects of post bleaching treatments to prevent restaining and the change of enamel surface microhardness after dental bleaching in vitro. Methods: Sixty intact human incisor teeth were stained in tea solution and randomly assigned into four groups (n=15). Then samples were bleached for two weeks (8 hours daily) by 15% carbamide peroxide. Tooth color was determined both with a spectrophotometer and visually before bleaching (T1) and immediately after bleaching (T2). Next, it was applied in group 1 fluoride (Naf 2%) gel for 2 minutes, and in group 2 a fractional CO2 laser (10 mJ, 200 Hz, 10 s), and in group 3, nanohydroxyapatite gel for 2 minutes. The bleached teeth in group 4 remained untreated (control group). Then teeth placed in tea solution again. Color examinations were repeated after various post bleaching treatments (T3) and restaining with tea (T4) and color change values recorded. The microhardness was measured at the enamel surface of samples. Data was analyzed using ANOVA, Tukey HSD test and Dunnett T3 (α = 0.05). Results: Directly after bleaching (ΔE T3-T2), the treatment with nanohydroxyapatite showed significantly the least color lapse in colorimetric evaluation. In experimental groups, the color change between T3 and T4 stages (ΔE T4-T3) was significantly lower than control group (P < 0.05). Different methods of enamel treatment caused a significant increase in surface microhardness compared to control group (P < 0.05). Significance: Application of fluoride, fractional CO2 laser and nanohydroxyapatite as post bleaching treatments are suggested for prevention of stain absorption and increasing the hardening of bleached enamel.

scholarly journals Effects of CO2laser irradiation on matrix-rich biofilm development formation–an in vitro study

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2458 ◽  
Author(s):  
Bruna Raquel Zancopé ◽  
Vanessa B. Dainezi ◽  
Marinês Nobre-dos-Santos ◽  
Sillas Duarte ◽  
Vanessa Pardi ◽  
...  
Keyword(s):  
Contact Angle ◽  
Biofilm Formation ◽  
Dental Enamel ◽  
In Vitro Study ◽  
Dry Weight ◽  
Vitro Study ◽  
Enamel Surface ◽  
Control Group ◽  
Biofilm Development

BackgroundA carbon dioxide (CO2) laser has been used to morphologically and chemically modify the dental enamel surface as well as to make it more resistant to demineralization. Despite a variety of experiments demonstrating the inhibitory effect of a CO2laser in reduce enamel demineralization, little is known about the effect of surface irradiated on bacterial growth. Thus, this in vitro study was preformed to evaluate the biofilm formation on enamel previously irradiated with a CO2laser (λ = 10.6 µM).MethodsFor this in vitro study, 96 specimens of bovine enamel were employed, which were divided into two groups (n = 48): 1) Control-non-irradiated surface and 2) Irradiated enamel surface. Biofilms were grown on the enamel specimens by one, three and five days under intermittent cariogenic condition in the irradiated and non-irradiated surface. In each assessment time, the biofilm were evaluated by dry weigh, counting the number of viable colonies and, in fifth day, were evaluated by polysaccharides analysis, quantitative real time Polymerase Chain Reaction (PCR) as well as by contact angle. In addition, the morphology of biofilms was characterized by fluorescence microscopy and field emission scanning electron microscopy (FESEM). Initially, the assumptions of equal variances and normal distribution of errors were conferred and the results are analyzed statistically by t-test and Mann Whitney test.ResultsThe mean of log CFU/mL obtained for the one-day biofilm evaluation showed that there is statistical difference between the experimental groups. When biofilms were exposed to the CO2laser, CFU/mL and CFU/dry weight in three day was reduced significantly compared with control group. The difference in the genes expression (Glucosyltransferases (gtfB) and Glucan-binding protein (gbpB)) and polysaccharides was not statically significant. Contact angle was increased relative to control when the surface was irradiated with the CO2laser. Similar morphology was also visible with both treatments; however, the irradiated group revealed evidence of melting and fusion in the specimens.ConclusionIn conclusion, CO2laser irradiation modifies the energy surface and disrupts the initial biofilm formation.

Fabrication and Bioactivity of Porous Titanium Implant

2007 ◽  
Vol 342-343 ◽  
pp. 613-616
Author(s):  
Xia Lu ◽  
Li Ang Xing ◽  
Pei Zhi Wang ◽  
Jun Fu
Keyword(s):  
Alkaline Phosphatase ◽  
Titanium Implant ◽  
Study Groups ◽  
Porous Titanium ◽  
Bone Sialoprotein ◽  
Control Group ◽  
Ha Coating ◽  
Titanium Material ◽  
Polymerase Chain

The aim of this study is to fabricate an implant framework for tissue engineering by sintering titanium beads and coating with hydroxyapatite and test its biocompatibility and bioactivity in vitro. The porous titanium with and without hydroxyapatite coating were involved in study groups. Osteoblastic proliferation, activity of alkaline phosphatase, mRNA of osteocalcin and bone sialoprotein were detected by MTT-assay, ALP test and real-time polymerase chain reaction respectively. The results indicated that the porous titanium material with/without HA coating could promote osteoblastic proliferation significantly contrast to the control group. However, only porous titanium with HA coating increased alkaline phosphatase, osteocalcin and bone sialoprotein gene expression apparently and had statistically differences with the other two groups. Abstract no. is TE-Po-044

scholarly journals AN INVITRO COMPARATIVE EVALUATION OF FRACTURE RESISTANCE OF ENDODONTICALLY TREATED TEETH OBTURATED WITH RESILON AND GUTTA-PERCHA

2014 ◽  
Vol 04 (03) ◽  
pp. 075-079
Author(s):  
Kiran Halkai ◽  
Rahul Halkai ◽  
Mithra N. Hegde ◽  
Vijay Kumar ◽  
Keyword(s):  
Fracture Resistance ◽  
Testing Machine ◽  
Control Group ◽  
Endodontically Treated Teeth ◽  
Group 4 ◽  
Anterior Teeth ◽  
Ah Plus ◽  
Group 3 ◽  
Group 2

Abstract Aim: To compare and evaluate in-vitro the fracture resistance of endodontically treated teeth obturated with resilon & epiphany sealer and guttapercha using different sealers. Epoxy resin based sealer AH-plus and zinc oxide eugenol based sealer-TubliSeal (EWT). Methodology: sixty four human single rooted maxillary anterior teeth, cleaned stored in 0.9% saline. All the teeth were decoronated to root length 14mm and bucco-lingual diameter of 5-7mm, After access openings teeth were instrumented using K3.06 up to final apical size 30/.06 and randomly allocated into 4 experimental groups (n=16 per group). Group 1(Control group): teeth were instrumented but not obturated, Group 2: Resilon cones and epiphany SE-sealer. Group 3: guttapercha cones and epoxy based sealer AH plus. Group 4: guttapercha and Tubli seal EWT. Coronal seal was done using IRM cement. Each of the specimens were tested for fracture resistance by instron universal testing machine. Results: Higher fracture resistance values were observed for group 2 (Resilon & Epiphany SE sealer) followed by group 3(Guttapercha & AH Plus sealer) and group 4(Guttapercha & TubliSeal EWT) when compared to group1 (control-instrumented but not obturated). Conclusion: filling the root canals with contemporary polymer based root canal obturating system- Resilon increased the in vitro fracture resistance of endodontically treated teeth.

scholarly journals Effect of a carbamide peroxide bleaching gel containing calcium or fluoride on human enamel surface microhardness

2005 ◽  
Vol 16 (2) ◽  
pp. 103-106 ◽  
Author(s):  
Rogério de Oliveira ◽  
Adriana Franco Paes Leme ◽  
Marcelo Giannini
Keyword(s):  
Artificial Saliva ◽  
In Vitro Study ◽  
Enamel Surface ◽  
Control Group ◽  
Carbamide Peroxide ◽  
Surface Microhardness ◽  
Treatment Groups ◽  
Peroxide Bleaching ◽  
Human Enamel

This in vitro study evaluated the surface microhardness of human enamel submitted to bleaching with 10% carbamide peroxide (CP) containing calcium or fluoride. Ninety-eight dental blocks (5 x 5 mm²) with polished enamel surfaces were randomly assigned to 7 treatment groups (n=14), as follows: without bleaching and storage in artificial saliva (control); 10% CP; 10% CP + 0.05% calcium; 10% CP + 0.1% calcium; 10% CP + 0.2% calcium; 10% CP + 0.2% fluoride; and 10% CP + 0.5% fluoride. During 14 days, enamel surfaces were daily exposed to a 6-h bleaching regimen followed by storage in artificial saliva. Surface microhardness was measured before (baseline), during (7th day), immediately after bleaching (14th day) and 1 week post bleaching. Data were analyzed by two-way ANOVA and Tukey's test (p<0.05). All treatments reduced SM significantly during the bleaching cycle (7th day), immediately after bleaching (14th day) and 1 week post bleaching, compared to baseline and to the unbleached control group. In conclusion, in spite of the addition of calcium and fluoride, all bleaching treatments affected the enamel surface microhardness.

scholarly journals Accuracy of Computer-Aided Dynamic Navigation Compared to Computer-Aided Static Procedure for Endodontic Access Cavities: An In Vitro Study

2020 ◽  
Vol 9 (1) ◽  
pp. 129 ◽  
Author(s):  
Álvaro Zubizarreta-Macho ◽  
Ana de Pedro Muñoz ◽  
Elena Riad Deglow ◽  
Rubén Agustín-Panadero ◽  
Jesús Mena Álvarez
Keyword(s):  
Navigation System ◽  
In Vitro Study ◽  
Study Groups ◽  
T Test ◽  
Anterior Teeth ◽  
Planning Software ◽  
Computer Aided ◽  
Dynamic Navigation ◽  
Group B

Purpose: To analyze the accuracy of two computer-aided navigation techniques to guide the performance of endodontic access cavities compared with the conventional access procedure. Materials and Methods: A total of 30 single-rooted anterior teeth were selected, which were randomly distributed into three study groups: Group A—guided performance of endodontic access cavities through computer-aided static navigation system (n = 10) (SN); Group B—guided performance of endodontic access cavities through computer-aided dynamic navigation system (n = 10) (DN); and Group C—manual (freehand) performance of endodontic access cavities (n = 10) (MN). The endodontic access cavities of the SN group were performed with a stereolithography template designed on 3D implant planning software, based on preoperative cone-beam computed tomography (CBCT) and a 3D extraoral surface scan, and endodontic access cavities of the DN group were planned and performed by the dynamic navigation system. After endodontic access cavities were performed, a second CBCT was done, and the degree of accuracy between the planned and performed endodontic access cavities was analyzed using therapeutic planning software and Student’s t-test. Results: Paired t-test revealed no statistically significant differences between SN and DN at the coronal (p = 0.6542), apical (p = 0.9144), or angular (p = 0.0724) level; however, statistically significant differences were observed between the two computer-aided navigation techniques and the MN group at the coronal (p < 0.0001), apical (p < 0.0001), and angular (p < 0.0001) level. Conclusion: Both computer-aided static and dynamic navigation procedures allowed accurate performance of endodontic access cavities.

scholarly journals Near-Surface Studies of the Changes to the Structure and Mechanical Properties of Human Enamel under the Action of Fluoride Varnish Containing CPP–ACP Compound

Biomolecules ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 765 ◽  
Author(s):  
Izabela Świetlicka ◽  
Damian Kuc ◽  
Michał Świetlicki ◽  
Marta Arczewska ◽  
Siemowit Muszyński ◽  
...  
Keyword(s):  
Group Treatment ◽  
In Vitro Study ◽  
Enamel Surface ◽  
Control Group ◽  
Near Surface ◽  
Molecular Arrangement ◽  
Human Enamel ◽  
Casein Phosphopeptides ◽  
Molar Teeth

Changes to the features of the enamel surface submitted to induced demineralisation and subsequent remineralisation were studied. The in vitro examination was conducted on polished slices of human molar teeth, divided in four groups: the untreated control (n = 20), challenged by a demineralisation with orthophosphoric acid (H3PO4) (n = 20), and challenged by a demineralisation following remineralisation with fluoride (F) varnish containing casein phosphopeptides (CPP) and amorphous calcium phosphate (ACP) compounds (n = 20). The specimens’ enamel surfaces were subjected to analysis of structure, molecular arrangement, mechanical features, chemical composition, and crystalline organization of apatite crystals. Specimens treated with acid showed a significant decrease in crystallinity, calcium, and phosphorus levels as well as mechanical parameters, with an increase in enamel surface roughness and degree of carbonates when compared to the control group. Treatment with fluoride CPP–ACP varnish provided great improvements in enamel arrangement, as the destroyed hydroxyapatite structure was largely rebuilt and the resulting enamel surface was characterised by greater regularity, higher molecular and structural organisation, and a smoother surface compared to the demineralised one. In conclusion, this in vitro study showed that fluoride CPP–ACP varnish, by improving enamel hardness and initiating the deposition of a new crystal layer, can be an effective remineralising agent for the treatment of damaged enamel.

scholarly journals Characterization of Fluorotic Enamel After Microabrasion and Desensitizing Agent

2017 ◽  
Vol 19 (1) ◽  
pp. 59
Author(s):  
V. Zavala-Alonso DDS, MSc,PhD ◽  
G. Roque-Márquez DDS, MSc ◽  
N. Patiño-Marín DDS, MSc, PhD ◽  
D. Silva-Herzog DDS, MSc, PhD
Keyword(s):  
Hydrochloric Acid ◽  
Surface Morphology ◽  
Dental Fluorosis ◽  
Study Groups ◽  
Superficial Layer ◽  
Enamel Surface ◽  
Anterior Teeth ◽  
Significant Difference ◽  
Desensitizing Treatment

Introduction. Enamel microabrasion is a procedure used for removing a superficial layer of enamel that has some alteration of color and/or texture caused by dental fluorosis. The purpose of this study was to compare the microhardness and micromorphology of the fluorotic enamel surface after microabrasion with 6.6% hydrochloric acid and silica or 18% hydrochloric acid and evaluate the effect of desensitizing agent exposure on the treated enamel.  Materials and Methods.  Twenty anterior teeth with moderate fluorosis were divided into two groups: 1) Perla-Dent® group and 2) Opalustre® group. Each buccal surface of incisors was sectioned to obtain samples 3x3 mm. The samples were then mounted in acrylic blocks. The enamel surface of the blocks was polished, after the microabrasion materials and desensitizing agent were applied according to the manufacturer's instructions. All samples were analyzed by Vickers microhardness tester and scanning electron microscopy (SEM). Results. Both experimental groups presented a decrease in the microhardness values, with statistically significant differences (p<0.0001) when comparing the baseline and after treatments values. To compare the microhardness values after both microabrasion and desensitizing treatment in the study groups, it was observed that the Perla-Dent® group obtained lower values than the Opalescence® group with a statistically significant difference (p<0.0001). The representative images of study groups in SEM showed the enamel surface morphology after Perla-Dent® treatment more irregular and a very marked relief than that observed in enamel surface morphology after Opalustre® treatment. Conclusion. The surface of the enamel was more affected with Perla-Dent® treatment than with Opalustre® treatment and the placement of UltraEz® agent does not recover its baseline microhardness.

scholarly journals Effects of CO2 laser irradiation on matrix-rich biofilm development formation – An in vitro study

2016 ◽  
Author(s):  
Bruna Raquel Zancope ◽  
Vanessa B Dainezi ◽  
Marinês Nobre-dos-Santos ◽  
Sillas Duarte, Jr ◽  
Vanessa Pardi ◽  
...  
Keyword(s):  
Contact Angle ◽  
Laser Irradiation ◽  
Co2 Laser ◽  
Biofilm Formation ◽  
In Vitro Study ◽  
Vitro Study ◽  
Enamel Surface ◽  
Control Group ◽  
Biofilm Development

Background. CO2 laser has been used to morphologically and chemically modify the dental enamel surface as well as to turn it more resistant to demineralization. Despite a variety of experiments demonstrating the inhibitory effect of CO2 laser in reduce enamel demineralization, little is known about the effect of surface irradiated on bacterial growth. Thus, this in vitro study was preformed to evaluate the biofilm formation on enamel previously irradiated with a CO2 laser (λ = 10.6 µM). Methods. For this in vitro study, it was employed 96 specimens of bovine enamel, which were divided into 2 groups (n = 48): 1) Control-non-irradiated surface and 2) Irradiated enamel surface. Biofilms were grown on the enamel specimens by 1, 3 and 5 days under intermittent cariogenic condition in the irradiated and non irradiated surface. In each assessment time, the biofilm were evaluated by dry weigh, counting the number of viable colonies and in fifth day, were evaluated by polysaccharides analysis, quantitative real time PCR as well as by contact angle. In addition, the morphology of biofilms was characterized by fluorescence microscopy and field emission scanning electron microscopy (FESEM). Initially, the assumptions of equal variances and normal distribution of errors were conferred and the results are analyzed statistically by t-test and Mann Whitney test. Results. The mean of log CFU/ml obtained for the 1-day biofilm evaluation showed that there is statistical difference between the experimental groups. When biofilms were exposed to CO2 laser, CFU/mL and CFU/ Dry Weight in 3 day was reduced significantly compared with control group. The difference in the genes expression (gtfB and gbpB) and polysaccharides was not statically significant. Contact angle was increased relative to control when the surface was irradiated with CO2 laser. Similar morphology was also visible with both treatments, however irradiated group revealed evidence of melting and fusion in the specimens. Conclusion. In conclusion CO2 laser irradiation modify the energy surface and disrupt the initial biofilm formation.

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