scholarly journals Evaluation of in- vitro Anti-inflammatory Potential of Grevillea robusta A. Cunn, EX R.BR. Leaves

2021 ◽  
Vol 68 (1) ◽  
Author(s):  
Samanta Jhuma ◽  
Kumar Vineet
Keyword(s):  
Side Effects ◽  
Chemical Constituents ◽  
Fibre Length ◽  
Ethanol Extract ◽  
Inflammatory Activity ◽  
Grevillea Robusta ◽  
Inflammatory Drugs ◽  
Anti Inflammatory ◽  
Microscopic Measurement

Synthetic Non-steroidal anti-inflammatory drugs (NASAIDs) are the choice of drug for inflammation. NASAIDs caused severe side effects like hyperacidity, gastric ulcer and so on. To avoid the side effects of NASAIDs, there is an urgent need for searching new molecule from natural origin. Present study is therefore aimed to explore Grevillea robusta A. Cunn, ex R.Br. Family proteaceae leaves for anti-inflammatory activity. Microscopic measurement (fibre length and width), Ash values and extractive values of Grevillea robusta leaves were determined to set the pharmacognostic standards. Chemical constituents were evaluated through chemical tests. The Ethanol extract of Grevillea robusta leaves (GRLE)) were subjected to evaluate in-vitro anti-inflammatory activity through HRBC method and Heat induced haemolytic method. The leaves of Grevillea robusta showed significant anti-inflammatory activity. The Ethanol extract (GRLE) showed significant anti-inflammatory activities. GRLE was found to contain polyphenols as chemical constituents which was the basis of anti-inflammatory activity. On the basis of result we can conclude that Ethanol extract of leaves of Grevillea robusta has good anti-inflammatory activity. GRLE could be used for treatment of inflammation.

scholarly journals Synthesis, Anti-Inflammatory Activity and Molecular Docking Studies of 1,4,5,6-Tetrahydropyrimidine-2-Carboxamides

2020 ◽  
Vol 27 (3) ◽  
pp. 353-365
Author(s):  
Volodymyr Ya. Horishny ◽  
Pavlo V. Zadorozhnii ◽  
Ivanna V. Horishnia ◽  
Vasyl S. Matiychuk
Keyword(s):  
Molecular Docking ◽  
Side Effects ◽  
Docking Studies ◽  
Inflammatory Activity ◽  
Cox Inhibitors ◽  
White Rats ◽  
Urgent Task ◽  
Inflammatory Drugs ◽  
Anti Inflammatory

Background: Nonsteroidal anti-inflammatory drugs (NSAIDs) are among the most commonly used drugs in the world. The widespread use of NSAIDs is associated with a number of serious side effects and complications observed for both selective and non-selective COX inhibitors. Therefore, the search for new COX inhibitors, which along with their effectiveness will have minimal side effects, is a very important and urgent task. Methods: This work studied the synthesis of new 1,4,5,6-tetrahydropyrimidine-2-carboxamides based on the reaction of 2-morpholin-4-yl-N-(het)aryl-2-thioxoacetamides with 1,3-diaminopropane. All obtained compounds were tested for anti-inflammatory activity in vitro and in silico conditions. All synthesized 1,4,5,6-tetrahydropyrimidine-2-carboxamides were tested for influence on the course of the exudative phase of the inflammatory process based on the carrageenan model of paw edema of laboratory nonlinear heterosexual white rats weighing 220-250 g, using Diclofenac as a reference. Optimization of the geometry of the studied structures and molecular docking was carried out using the ArgusLab 4.0.1 software package. Results: The target products were obtained with yields of 71-98% and easily isolated from the reaction mixture. The best anti-inflammatory activity was found in N-(4-chlorophenyl)-1,4,5,6-tetrahydropyrimidine-2-carboxamide and in N-[4-chloro-3-(trifluoromethyl)phenyl]-1,4,5,6-tetrahydropyrimidine-2-carboxamide, suppression of the inflammatory response was 46.7 and 46.4%, respectively. The results of molecular docking with COX-1 and COX-2 enzymes were in good agreement with the experimental data, R2 ˃ 0.92 and R2 ˃ 0.83, respectively. Conclusion: The compounds under study were shown to be promising as potential anti-inflammatory agents.

scholarly journals A REVIEW ON IN VIVO AND IN VITRO EXPERIMENTAL MODELS TO INVESTIGATE THE ANTI-INFLAMMATORY ACTIVITY OF HERBAL EXTRACTS

2018 ◽  
Vol 11 (11) ◽  
pp. 29
Author(s):  
Inayat Kabir ◽  
Imtiyaz Ansari
Keyword(s):  
Reference Model ◽  
Experimental Models ◽  
Inflammatory Activity ◽  
Herbal Extracts ◽  
Double Positive ◽  
Arthritis Models ◽  
Inflammatory Drugs ◽  
Anti Inflammatory

The article emphasizes the anti-inflammatory effects of herbal extracts on different experimental models that are repeatedly used to test the in vivo anti-inflammatory activity of herbal components. Edema, granuloma and arthritis models are used to test the anti-inflammatory activity of plant extracts whereas formalin or acetic acid-induced writhing test and hot plate methods are the most repeatedly used to evaluate anti-nociceptive potentials of the herbal extracts. Although adjuvant-induced and collagen-induced arthritis models are also quite efficient, they have been used seldom to evaluate anti-inflammatory tendencies of the herbs. Here, we suggest a double positive reference model using both steroid and nonsteroidal anti-inflammatory drugs at the same time, instead of using only one of them either.

scholarly journals Phenanthrenes in Chinese Yam Peel Exhibit Anti-inflammatory Activity, as Shown by Strong in Vitro Cyclooxygenase Enzyme Inhibition

2016 ◽  
Vol 11 (9) ◽  
pp. 1934578X1601100 ◽  
Author(s):  
Qian Li ◽  
Chuan-Rui Zhang ◽  
Amila A. Dissanayake ◽  
Qun-yu Gao ◽  
Muraleedharan G. Nair
Keyword(s):  
Food Item ◽  
Inflammatory Activity ◽  
Spectroscopic Methods ◽  
Over The Counter ◽  
Chinese Yam ◽  
Inflammatory Drugs ◽  
In Vitro Bioassays ◽  
Anti Inflammatory ◽  
Cox 1

Chinese yam ( Dioscorea opposita), peeled or whole, is a popular food item that is considered to be healthy. Often, the yam is peeled before cooking. However, it is also consumed with peel. Therefore, in this study, the peel of this yam was extracted sequentially with n-hexane, ethyl acetate and methanol, and studied for its health-benefits, using in vitro bioassays. Bioactivity-guided purifications of extracts of the peel afforded phenanthrenes (1-4), as characterized by spectroscopic methods. Phenanthrene 1 is a novel analogue. The extracts and isolates were tested for anti-inflammatory activity using cyclooxygenase enzyme (COX-1 and -2) inhibitory assays. All phenanthrenes isolated from the yam peel showed higher inhibition of COX enzymes than the over-the-counter nonsteroidal anti-inflammatory drugs (NSAIDs) aspirin, ibuprofen and naproxen.

scholarly journals Preliminary Analysis of the Anti-Inflammatory Activity of Essential Oils of Zingiber zerumbet

2010 ◽  
Vol 13 (4) ◽  
pp. 425-432 ◽  
Author(s):  
Z. A. Zakaria ◽  
A. S. Mohamad ◽  
M. S. Ahmad ◽  
A. F. Mokhtar ◽  
D. A. Israf ◽  
...  
Keyword(s):  
Side Effects ◽  
Essential Oil ◽  
Preliminary Analysis ◽  
Formalin Test ◽  
Inflammatory Activity ◽  
Paw Edema ◽  
Zingiber Zerumbet ◽  
Cotton Pellet ◽  
Inflammatory Drugs ◽  
Anti Inflammatory

Nonsteroidal anti-inflammatory drugs (NSAIDs) have been widely used for the treatment of inflammation. However, despite their effectiveness, most NSAIDs cause various side effects that negatively affect the management of inflammation and, in part, pain. Thus, there is a need to search for new anti-inflammatory agents with few, or no, side effects. Natural products of plant, animal, or microorganism origin have been good sources of new bioactive compounds. The present study was carried out to evaluate the acute and chronic anti-inflammatory activities of the essential oil of the rhizomes of Zingiber zerumbet (Zingiberaceae) using the carrageenan-induced paw edema and cotton pellet-induced granuloma tests, respectively. The effect of the essential oil on inflammatory- and noninflammatory-mediated pain was also assessed using the formalin test. Essential oil of Z. zerumbet, at doses of 30, 100, and 300 mg/kg, was administered intraperitoneally to rats. The substance exhibited significant anti-inflammatory activity both in acute and chronic animal models. The essential oil also inhibited inflammatory- and noninflammatory-mediated pain when assessed using the formalin test. In conclusion, the essential oil of Z. zerumbet possessed anti-inflammatory activity, in addition to its antinociceptive activity, which may explain its traditional uses to treat inflammatory-related ailments.

scholarly journals A Glycosaminoglycan-Rich Fraction from Sea Cucumber Isostichopus badionotus Has Potent Anti-Inflammatory Properties In Vitro and In Vivo

Nutrients ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 1698
Author(s):  
Leticia Olivera-Castillo ◽  
George Grant ◽  
Nuvia Kantún-Moreno ◽  
Hirian A. Barrera-Pérez ◽  
Jorge Montero ◽  
...  
Keyword(s):  
Sea Cucumber ◽  
Inflammatory Responses ◽  
Ethanol Extract ◽  
Inflammatory Activity ◽  
Bioactive Constituents ◽  
Mouse Ear ◽  
Health Promoting ◽  
Anti Inflammatory

Sea cucumber body wall contains several naturally occurring bioactive components that possess health-promoting properties. Isostichopus badionotus from Yucatan, Mexico is heavily fished, but little is known about its bioactive constituents. We previously established that I. badionotus meal had potent anti-inflammatory properties in vivo. We have now screened some of its constituents for anti-inflammatory activity in vitro. Glycosaminoglycan and soluble protein preparations reduced 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammatory responses in HaCaT cells while an ethanol extract had a limited effect. The primary glycosaminoglycan (fucosylated chondroitin sulfate; FCS) was purified and tested for anti-inflammatory activity in vivo. FCS modulated the expression of critical genes, including NF-ĸB, TNFα, iNOS, and COX-2, and attenuated inflammation and tissue damage caused by TPA in a mouse ear inflammation model. It also mitigated colonic colitis caused in mice by dextran sodium sulfate. FCS from I. badionotus of the Yucatan Peninsula thus had strong anti-inflammatory properties in vivo.

scholarly journals Sorbaria kirilowii Ethanol Extract Exerts Anti-Inflammatory Effects In Vitro and In Vivo by Targeting Src/Nuclear Factor (NF)-κB

Biomolecules ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 741 ◽  
Author(s):  
Jiwon Jang ◽  
Jong Sub Lee ◽  
Young-Jin Jang ◽  
Eui Su Choung ◽  
Wan Yi Li ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Ex Vivo ◽  
Ethanol Extract ◽  
Inflammatory Activity ◽  
No Production ◽  
Nuclear Factor ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Inflammation is a fundamental process for defending against foreign antigens that involves various transcriptional regulatory processes as well as molecular signaling pathways. Despite its protective roles in the human body, the activation of inflammation may also convey various diseases including autoimmune disease and cancer. Sorbaria kirilowii is a plant originating from Asia, with no anti-inflammatory activity reported. In this paper, we discovered an anti-inflammatory effect of S. kirilowii ethanol extract (Sk-EE) both in vivo and in vitro. In vitro effects of Sk-EE were determined with lipopolysaccharide (LPS)-stimulated RAW264.7 cells, while ex vivo analysis was performed using peritoneal macrophages of thioglycollate (TG)-induced mice. Sk-EE significantly reduced the nitric oxide (NO) production of induced macrophages and inhibited the expression of inflammation-related cytokines and the activation of transcription factors. Moreover, treatment with Sk-EE also decreased the activation of proteins involved in nuclear factor (NF)-κB signaling cascade; among them, Src was a prime target of Sk-EE. For in vivo assessment of the anti-inflammatory effect of Sk-EE, HCl/EtOH was given by the oral route to mice for gastritis induction. Sk-EE injection dose-dependently reduced the inflammatory lesion area of the stomach in gastritis-induced mice. Taking these results together, Sk-EE exerts its anti-inflammatory activity by regulating intracellular NF-κB signaling pathways and also shows an authentic effect on reducing gastric inflammation.

scholarly journals Aktifitas Kunyit (Curcuma domestica Val.) Sebagai Antiinflamasi Ditinjau Dari Berbagai Literatur

2021 ◽  
Vol 5 (1) ◽  
pp. 102
Author(s):  
Humaira Fadhilah ◽  
Karunia Rachmani ◽  
Nurihardianti Hajaring
Keyword(s):  
Side Effects ◽  
Tissue Injury ◽  
Inflammatory Activity ◽  
Literature Study ◽  
Induction Method ◽  
Protective Response ◽  
Physical Trauma ◽  
Inflammatory Drugs ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Inflammation is a normal protective response to tissue injury caused by physical trauma, damaging chemicals, or microbiological substances. Steroids and nonsteroidal anti-inflammatory drugs have many side effects, so there are many anti-inflammatory developments originating from natural ingredients, especially in plants. Plants that are scientifically proven to have anti-inflammatory properties, namely turmeric (Curcuma domestica Val.) The method used in this literature study is a review of various journals published online, with the keyword turmeric as an anti-inflammatory, reviewed one by one and then the journals obtained are collected and information created by summarizing the content and then comparing the journals to be used as references. The results showed that turmeric tested had anti-inflammatory activity. The strength of the anti-inflammatory effect is shown by the carrageenan induction method which inhibits endema in rat feet and inflammation in the liver using the method of induction by diethylnitrosamine in this plant varies, depending on the dose. Compounds that are considered to provide anti-inflammatory activity are curcumin class compounds because they can inhibit the formation of prostagladin, thromboxan, and prostagycycline by inhibiting the activity of the cyclooxygenase enzyme. Curcumin also inhibits the formation of leuketrien compounds by inhibiting the activity of the lipoxygenase enzyme.Keywords: Anti-inflamatoryTurmericCucurminABSTRAK Inflamasi merupakan perlindungan normal ketika timbul luka jaringan karena zat mikrobiologi, zat kimia atau trauma fisik. Efek samping yang ditimbulkan oleh obat antiinflamasi banyak sehingga dibuat bahan alam untuk pengembangan antiinflamasi salah satunya adalah tanaman. Kunyit (Curcumma domestica Val.) adalah tanaman yang memiliki khasiat antiinflamasi yang terbukti secara ilmiah. Metode yang digunakan adalah studi literatur dengan  kata kunci kunyit sebagai antiinflamasi, diulas satu persatu kemudian jurnal yang didapat dikumpulkan dan informasi dibuat dengan merangkum isi lalu membandingkan jurnal yang akan dijadikan acuan. Hasil membuktikan bahwa kunyit memiliki aktivitas antiinflamasi. Efek antiinflamasi ditunjukkan dengan metode induksi karagenan yg menghambat endema pada  kaki tikus dan peradangan pada hati tikus menggunakan metode induksi oleh dietilnitrosamin tergantung dosisnya pada tanaman berbeda. Senyawa golongan kurkumin merupakan senyawa yang terbukti memberikan aktivitas antiinflamasi karena dapat menghambat pembentukan prostagladin, prostagsiklin dan tromboksan dengan cara menghambat aktifitas enzim siklooksigenase. Aktivitas yang lain dari kurkumin adalah menghambat pembentukan senyawa leuketrien dengan menghambat aktifitas enzim lipoxygenase.Kata Kunci: AntiinflamasiKunyitKukurmin

scholarly journals Inhibitory effects of hydroethanolic extracts from three Cameroonian medicinal plants on proteins inflammation and growth of multi-resistant strains of Mycobacterium tuberculosis

2021 ◽  
Vol 11 (4-S) ◽  
pp. 15-21
Author(s):  
Esther Del florence Ndedi Moni ◽  
Patrick Hervé Diboue Betote ◽  
Christelle Wayoue Kom ◽  
Chimène Félicite Mekoulou Benga ◽  
Armelle Deutou Tchamgoue ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Medicinal Plants ◽  
Inhibitory Action ◽  
Ethanol Extract ◽  
Inflammatory Activity ◽  
Phytochemical Screening ◽  
Resistant Strains ◽  
Anti Inflammatory ◽  
Ethanol Extracts

The present work aimed to determine the phytochemical components and evaluate the in vitro anti-inflammatory and anti-mycobacterial effects of hydroethanolic extracts of Allium sativum L bulbs, Drypetes gossweileri S. MOORE stem-barks and Pentadiplandra brazzeana Baill roots against several resistant strains of Mycobacterium tuberculosis. The phytochemical screenings of extracts were carried out according the colorimetric and precipitation tests to reveal the presence of phytochemical compounds. The anti-inflammatory effects of extracts were evaluated using in vitro Bovine Serum Albumin denaturation and proteinase inhibitory action assays. The inhibitory parameters of hydro-ethanol extracts were evaluated by the microdilution method agaisnt Mycobacterium tuberculosis. The phytochemical screening of hydro-ethanol extracts revealed the presence of phenols, polyphenols, flavonoids, alkaloids, cathechic tannins, triterpens, steroids, anthocyanins and leucoanthocyanins. The anti-inflammatory activity of hydro-ethanol extracts of D. gossweileri, P. brazzeana and A. sativum have shown the inhibitory concentrations 50 (IC50) values ranging from 356.70, 183.30 and 226.30 mg/mL for BSA denaturation and 31.92, 33.62 and 56.93 mg/mL for proteinase inhibitory action respectively. The hydroethanolic extracts of D. gossweileri, P. brazzeana and A. sativum exhibited moderate and weak anti-mycobacterial activities with the minimum inhibitory concentrations (MICs) ranging from 312.5 to 2500 μg/mL. A. sativum hydro-ethanol extract has shown the highest anti-mycobacterial activity with MIC of 312.5 μg/mL against isoniazid resistant of M. tuberculosis and extremely resistant drug strain of M. tuberculosis. These results suggest that hydro-ethanol extracts of A. sativum, D. gossweileri and P. brazzeana are efficient against tuberculosis caused by multi-resistant Mycobacterium tuberculosis strains and are able to resorb the inflammation induced during infection. Keywords: Anti-inflammatory activity, Anti-mycobacterial effect, Hydroethanolic extracts, Medicinal plants, Phytochemical screening.

scholarly journals Phytochemical research and anti-inflammatory activity of the dry extracts from northern highbush blueberry leaves

2021 ◽  
pp. 40-48
Author(s):  
Oleksandr Stremoukhov ◽  
Oleh Koshovyi ◽  
Mykola Komisarenko ◽  
Igor Kireyev ◽  
Andriy Gudzenko ◽  
...  
Keyword(s):  
Amino Acids ◽  
Phenolic Compounds ◽  
Side Effects ◽  
New Drugs ◽  
Inflammatory Activity ◽  
Highbush Blueberry ◽  
Northern Highbush Blueberry ◽  
Inflammatory Drugs ◽  
Anti Inflammatory ◽  
Blueberry Leaves

All over the world, non-steroidal anti-inflammatory drugs (NSAIDs) are taken annually by about three hundred million people and this figure is constantly increasing. At the same time, NSAIDs are also one of the most common causes of side effects of drug therapy. The development and implementation of new anti-inflammatory drugs, including those of plant origin, with minimal side effects is an urgent task of modern pharmaceutical science. Vaccinium corymbosum L. (family Ericaceae), which is gaining more and more popularity among berry crops and is successfully cultivated in Ukraine, is promising in this direction for research. The aim: phytochemical analysis of dry extracts from blueberry leaves to establish the possibility of creating new drugs with anti-inflammatory activity. Materials and methods. The objects of the study were dry extracts of northern highbush blueberry leaves. The content of amino acids and phenolic compounds was determined by HPLC and spectrophotometry. The prototypal activity was studied in vivo and in vitro. Research results. 4 dry extracts were obtained from northern highbush blueberry leaves. In the extracts obtained by HPLC, 7 amino acids were identified, including 3 essential ones: arginine, histidine, and phenylalanine. As a result of the HPLC study, 7 phenolic compounds were identified in extracts from the leaves of northern highbush blueberry: 5 flavonoids - rutin, quercetin-3-O-glucoside, kaempferol-3-O-glucoside, quercetin and kaempferol and 2 hydroxycinnamic acids, chlorogenic and caffeic acid. For the first time, the anti-inflammatory effect of extracts from blueberry leaves was investigated. It was revealed that extract 1 at a dose of 50 mg/kg and extract 4 modified with arginine at a dose of 25 mg/kg have the highest anti-inflammatory activity. Conclusions. The results of the conducted studies indicate that extracts from the leaves of northern highbush blueberry in terms of the content of biologically active substances are promising sources for the creation of new drugs and dietary supplements with anti-inflammatory activity

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