scholarly journals Preventive Role of Zingiber Officinale against Hyperglycemia in Alloxan Induced Diabetic Rats

2019 ◽  
Vol 30 (Number 1) ◽  
pp. 26-29
Author(s):  
S Sultana ◽  
N Y Mili ◽  
R Afroz ◽  
S Parveen
Keyword(s):  
Blood Glucose ◽  
Zingiber Officinale ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Standard Diet ◽  
Group Iii ◽  
Diabetic Control Group ◽  
Glucose Estimation ◽  
Preventive Role

The experimental animal study was undertaken to investigate the preventive role ginger juice against hyperglycemia in alloxan induced diabetic rats.Male wistar rats,(130-150)gm wt fed on standard diet and water ad libitum, were divided into 3 groups(n=6) in each group: Group-L non-diabetic control group, Group-II, diabetic control & Group-III, normal rats pretreated with ginger before they were made diabetics. Diabetes was induced by inj. alloxan 150mg/kg body wt.,tp (Group-IL on 2nd day & Group-Ill, on the 9th day).Rats having blood glucose level of more than 7mmol/L on day 5(72 hours after alloxan inj) were considered diabetic & selected for experiment. Rats of Group-Ill received Zingiber officinale (ginger juice) (4m1/kg.body,wt orally) for 7 days (day 2-day8) through Pyles tube before alloxan induction & 3days after the induction. On day 12, animals were sacrificed under light ether anaesthesia, blood was collected by cardiac puncture for blood glucose estimation. Pretreatment with Zingiber officinale (ginger) juice significantly (p<0.01) reduced alloxan induced hyperglycemia.Zingiber officinale (ginger) is one of the most widely used spices and is reputed to have medicinal properties against diabetes mellitus. This study suggests that pretreatment with Zingiber officinale(ginger) prevents the development of hyperglycemia in alloxan induced diabetic rats.

scholarly journals Comparative study of the effect of ethanolic extract of swietenia mahagoni seeds with rosiglitazone on experimentally induced diabetes mellitus in rats

2013 ◽  
Vol 39 (1) ◽  
pp. 6-10
Author(s):  
SMMMA Hasan ◽  
MI Khan ◽  
BU Kumar ◽  
MZ Sadeque
Keyword(s):  
Blood Glucose ◽  
Ethanolic Extract ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Diabetic Control Group ◽  
Group B ◽  
Group D ◽  
Swietenia Mahagoni ◽  
Experimentally Induced

The study was performed to compare the blood glucose lowering effect of Swietenia mahagoni seeds with an oral antidiabetic drug, rosiglitazone in experimentally induced diabetic rats. Twentyfour healthy Long Evans Norwegian strain of rats were included in the study and divided into four groups (A, B, C and D) comprising 6 rats each. Group A (control group) received standard rat food for 14 days. Diabetes was induced by a single intraperitoneal administration of alloxan 120mg/kg body weight in Group B, C and D. Group B was given standard food for 10 days and considered as diabetic control. Group C and D were treated with ethanolic extract of Swietenia mahagoni seeds 1000mg/kg and rosiglitazone 10mg/kg orally respectively. Administration of ethanolic extract of Swietenia mahagoni seeds in group C and rosiglitazone in group D produced a significant reduction in blood glucose level as compared to diabetic control (group B). Histological examination of pancreas showed destruction of beta cells in Islets of pancreas in group B whereas retaining of islets and few degranulations of beta cells of pancreas found in group C and group D. The observations and results of the present study provide information that ethanolic extract of Swietenia mahagoni seeds has hypoglycaemic effect in experimentally induced diabetic rats which requires further investigation. DOI: http://dx.doi.org/10.3329/bmrcb.v39i1.15790 Bangladesh Med Res Counc Bull 2013; 39: 6-10

scholarly journals Pharmacodynamics Drug Interactions of Metformin with Aspirin and Nifedipine

2016 ◽  
Vol 8 (1) ◽  
pp. 4 ◽  
Author(s):  
Khidir A. M. Hassan ◽  
Mahmoud M. E. Mudawi ◽  
Mansour I. Sulaiman
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Diabetic Control ◽  
Serum Glucose ◽  
Diabetic Rats ◽  
Control Group ◽  
Citrate Buffer ◽  
Treatment Groups ◽  
Diabetic Control Group

Metformin is now being recognized as the standard therapy in T2D patients who are overweight. Metformin has many drug-disease interactions that can increase the risk of metformin-associated lactic acidosis. Therefore this study was conducted to evaluate any possible pharmacodynamic interactions between metformin and drugs used to treat chronic diseases e.g. Hypertension. The rats were fasted overnight before inducing diabetes with streptozotocin. The rats were given an intraperitoneal injection of streptozotocin (50 mg kg−1) freshly prepared in 0.1M sodium citrate buffer. The diabetic state was confirmed 72 h after streptozotocin injection. Diabetic rats were grouped into seven groups each group of five rats and distributed among the normal control group diabetic control group and the treatment groups. The treatment continued for 10 days. Blood samples were taken before treatment and after 10 days and analyzed for serum glucose, cholesterol, HDL, LDL, and triglycerides. In the diabetic control group which was given STZ alone the blood glucose level decreased significantly (p &lt; 0.05) after 10 days but still above the hyperglycemic level (200mg/dl). The same was observed in the group treated with metformin. The group treated with nifedipine and aspirin showed significant reduction (p &lt; 0.01) in the glucose level below the hyperglycemic level (200mg/dl). While the groups treated with (Metformin + Nifedipine) and (Metformin +Aspirin) showed highly significant reduction (P&lt;0.001) in blood glucose level. These results conclude that the combination of (metformin +Nifedipine) and the combination of (Metformin + Aspirin) have highly significant hypoglycemic effect. It also showed that Nifedipine has promising role in reducing blood glucose level, lipid profile especially LDL-cholesterol, and body weight.

scholarly journals THE EFFECTIVENESS OF ETHANOL EXTRACT OF BINAHONG LEAVES ON DIABETIC WOUND HEALING

2018 ◽  
Vol 11 (4) ◽  
Author(s):  
Devita Anggraeni ◽  
Claude Mona Airin ◽  
Slamet Raharjo
Keyword(s):  
Blood Glucose ◽  
Leukocyte Count ◽  
Ethanol Extract ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Dorsal Skin ◽  
Group I ◽  
Diabetic Control Group ◽  
Chemical Profiles

This research aimed to study the effectiveness of ethanol extract of binahong leaves on blood glucose, insulin, blood chemical profiles (serum glutamic pyruvate transaminase=SGPT, serum glutamic oxaloacetic transaminase=SGOT, ureum, and creatinine), and skin histopathology in diabetic rat. A total of 20 male Wistar rats aged 3 months (± 250 gram) were divided into five groups, with four rats in each group. Group I (non-diabetic control) was injected with 0.1 M sodium citrate buffer, while group II (diabetic control), III, IV, and V were injected with single dose of Streptozotocin (STZ) at dose 40 mg/kg intraperitoneally (IP). One week after the injection, the dorsal skin of the rats were excised. Group I and II were given cream topically and 1% NaCMC orally, group III was given 50% ethanol extract of binahong leaves (EEB) topically and 1% NaCMC orally, group IV was given cream topically and EEB 300 mg/kg orally, and group V was given 50% EEB topically and EEB 300 mg/kg orally. These treatments were continued for 14 days. Blood samples were obtained at the end of study to examine blood glucose, insulin, and blood chemical profiles (SGOT, SGPT, ureum, and creatinine). Examination of skin histopathology and leukocyte count were also done. The result showed that blood glucose, insulin, SGOT, SGPT, and ureum level of diabetic rats given topical or oral EEB did not significantly different from diabetic control group, even though blood glucose, insulin, SGOT, SGPT, and ureum level of diabetic rats given topical and oral EEB were found lower compared to diabetic control group. Administration of EEB 300 mg/kg orally in diabetic rats could lower creatinine level significantly (P<0.05). Histopathological examination of dorsal skin of diabetic rats which were given EEB topically showed the decrease of fibroblast proliferation, leukocyte infiltration, and hemorrhage in dermis area. Leukocyte count on skin tissue was significantly lower (P<0.05) in diabetic rats given EEB. In conclusion, topical or oral administration of EEB can help healing process in diabetic wound.

scholarly journals ANTIDIABETIC AND ANTIHYPERLIPIDAEMIC EFFECTS OF THE ETHANOL EXTRACT OF THE LEAVES AND STEM OF CISSUS GRACILLIS (GULL ET PERR) (VITACEAE)

2021 ◽  
pp. 54-56
Author(s):  
OLUSAYO A SHORINWA ◽  
GORDON EI EMENU
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Albino Rats ◽  
Phytochemical Screening ◽  
Glucose Levels ◽  
Diabetic Control Group ◽  
Alloxan Monohydrate

Objectives: This study investigated the antidiabetic and antihyperlipidemic potential of the ethanol extract of the leaves and stem of Cissus gracillis on alloxan monohydrate-induced diabetic albino rats. Methods: Preliminary phytochemical screening and acute toxicity were carried out. Animals were assigned into seven groups of five rats each. Groups A and B were administered 10 mg/kg each of glibenclamide and atorvastatin respectively, C, D, and E were given 125, 250 and 500 mg/kg of ethanol extract of C. gracillis, respectively, daily for 21 days through oral gavage, group F was diabetic but untreated (diabetic control group), while group G was non-diabetic and untreated which served as the control group. Results: Phytochemical screening revealed the presence of steroids/triterpenoids and carbohydrates. LD50 was above 5000 mg/kg. The extract at 500 mg/kg showed a statistically significant (p<0.05) decrease in blood glucose level when compared with the glibenclamide group on day 21. However, gradual non- significant reduction in blood glucose levels were observed in the extract treated groups on the 7th, 14th, and 21st days of treatment. The administration of ethanol extract of C. gracillis to alloxan-induced diabetic rats produced a decrease in total cholesterol, triglycerides, and low-density lipoproteins comparable to glibenclamide and atorvastatin. Conclusion: The ethanol extract of the leaves and stem of C. gracillis possess a mildly significant antidiabetic and antihyperlipidemic activity.

scholarly journals Evaluation of Antidiabetic Activity of Polyherbal Formulation “Vasant Kusumakar Ras” on Alloxan-induced and Dexamethasone-induced Diabetic Rats

2021 ◽  
pp. 13-22
Author(s):  
Talha Jawaid ◽  
Kumari Nishu ◽  
Mehnaz Kamal ◽  
Saud M. Alsanad
Keyword(s):  
Blood Glucose ◽  
Group Treatment ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Albino Rats ◽  
Reference Drug ◽  
Polyherbal Formulation ◽  
Diabetic Control Group

Aim: The current study observed the antidiabetic effect of Vasant Kusumakar Ras, an Ayurvedic polyherbal formulation, in alloxan-induced and dexamethasone-induced diabetic rats. Materials and Methods: Alloxan (120 mg/kg, i.p.) and dexamethasone sodium phosphate (5 mg/kg, i.p.) were used to induce diabetes in rats. The oral antidiabetic activity of Vasant Kusumakar Ras was evaluated by single doses of Vasant Kusumakar Ras (400 and 600 mg/kg, p.o.) in albino rats during a 10-day treatment period, with the effect of the Vasant Kusumakar Ras on blood glucose levels and serum lipid parameters measured on 0, 7th, and 11th day. Glibenclamide (5 mg/kg, p.o.) was used as the reference drug. Results: In alloxan-induced diabetic rats, the elevated levels of blood glucose significantly (p < 0.05) decreased after oral administration of Vasant Kusumakar Ras (400 mg/kg and 600 mg/kg), and Glibenclamide (5 mg/kg). When compared to the diabetic control group, treatment with Vasant Kusumakar Ras and Glibenclamide for 10 days reduced total cholesterol (TC) significantly (p < 0.001). Treatment with Vasant Kusumakar Ras and Glibenclamide for 10 days, significantly (p < 0.001) decreased low-density lipoprotein (LDL) level when compared to the diabetic control group. In dexamethasone-induced diabetic rats, all rats given with dexamethasone and Vasant Kusumakar Ras (400 mg/kg and 600 mg/kg) showed a significant (p < 0.05) decrease in the level of blood glucose when compared with diabetic control rats. The rats treated with dexamethasone and Glibenclamide showed a significant (p < 0.05) decrease in blood glucose level when compared to diabetic control rats. When compared to the diabetic control group, treatment with Vasant Kusumakar Ras and Glibenclamide (5 mg/kg) for 10 days reduced TC significantly (p < 0.001). Treatment with Vasant Kusumakar Ras and Glibenclamide for 10 days, significantly (p < 0.001) decreased LDL level when compared to the diabetic control group. Conclusion: Vasant Kusumakar Ras was shown to have significant antidiabetic activity comparable to that of glibenclamide and it also improves the lipid metabolism in both alloxan-induced and dexamethasone-induced diabetic rats.

scholarly journals Effects of Silymarin on Blood Glucose Concentration, Hepatic Histopathological Changes and FOXA2 and FOXA3 Gene Expression in Streptozotocin-Induced Diabetic Male Wistar Rats

2021 ◽  
Vol 5 (2) ◽  
pp. 17
Author(s):  
Sajad Nikkhah ◽  
Rahman Jafari Hafshejan ◽  
Farshid Gheibi Hajivar ◽  
Khalil Khashei ◽  
Sara Afzali
Keyword(s):  
Gene Expression ◽  
Blood Glucose ◽  
Glucose Concentration ◽  
Blood Glucose Concentration ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Histopathological Changes ◽  
Diabetic Control Group ◽  
Male Wistar Rats

Since the liver is among the primary organs susceptible to the effects of hyperglycaemia, diabetes mellitus (DM) could be a risk factor for the development and progression of liver damage. In present study, since no side-effects from the herbal medicine have been reported, the effect of silymarin on blood glucose concentration, hepatic histopathological changes and FOXA2 and FOXA3 gene expression, which are key genes in liver regeneration, was investigated. In this fundamental with experimental approach study, 40 male Wistar rats weighing 180-220 g were used. Rats were kept under the standard conditions of temperature of 20-22°C and humidity of 50% and consecutive 12-hour periods of light and darkness. Rats were randomly divided into five different groups (n=8 each), including healthy control rats, diabetic control rats, diabetic rats receiving silymarin (50, 100 and 150 mg/kg). Diabetes was induced by injecting streptozotocin (50 mg/kg B.W., i.p.). For 4 weeks silymarin groups received the drug once every three days through gavage and fasting blood glucose concentration measured once every 10 days. At the end of a month experiment, livers were harvested for hepatic histopathological and FOXA2 and FOXA3 gene expression changes analysis. In the diabetic rats treated with silymarin (50, 100 and 150 mg/kg), by comparison with the diabetic control group (p<0.05), glucose levels decreased significantly. Moreover, FOXA2 and FOXA3 expression in diabetic groups treated with silymarin significantly increased compared to diabetic control group (p<0.05). Hepatic histopathological changes were improved in the treated groups.The present study indicates that silymarin significantly decreased blood glucose concentration and increased the FOXA2 and FOXA3 gene products level. Hence, silymarin is able to improve some of the symptoms associated with diabetes and possesses hepatoprotective effects in streptozotocin-induced diabetic rats.

Accelerative Wound-Healing Effect of Aqueous Anthocephalus Cadamba Leaf Extract in a Diabetic Rat Model

2021 ◽  
pp. 153473462110183
Author(s):  
Shoket Ali ◽  
Sharmeen Ishteyaque ◽  
Foziya Khan ◽  
Pragati Singh ◽  
Abhishek Soni ◽  
...  
Keyword(s):  
Wound Healing ◽  
Leaf Extract ◽  
Povidone Iodine ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Group Iv ◽  
Control Group ◽  
Group Iii ◽  
Group I ◽  
Diabetic Control Group

Impaired wound healing is a major concern in diabetic patients due to unregulated chronic hyperglycemia which further may lead to ulcer, gangrene, and its complications. The present study unveils the accelerative effect of aqueous Anthocephalus cadamba leaf extract on wound healing in diabetic rats. Diabetes was induced in 30 Sprague Dawley female rats by using streptozotocin (except control group I) at the dose of 60 mg/kg intraperitoneally. Diabetic rats were randomized in 3 groups viz. diabetic control group (II), diabetes + Kadam plant leaf extract group (III), and diabetes + 5% povidone–iodine solution group (IV). Surgically sterile wound of 1.77 cm2 was created on the dorsal area of anaesthetized rats. The experimental parameters were assessed by hematobiochemical, histopathological, and western blot techniques. The A cadamba extract treatment group (III) (D + KPLE) showed a significant increase in the percentage of wound closure (82%) at day 21 as compared to the diabetic control group (42%), nondiabetic control group (I) (49%), and povidone–iodine treatment group (75%) group (IV). The findings of the present study suggest that the (D + KPLE) group (III) exhibited marked epithelial regeneration, neovascularization, collagen deposition, and fibroblast proliferation along with higher expression of vascular endothelial growth factor as compared to the diabetic control group (II), which was confirmed by histopathological examination and western blot analysis. The present study suggests that the topical application of aqueous A cadamba leaf extract exhibits accelerative wound-healing properties in diabetic rats.

scholarly journals Ameliorative Activity of Pumpkin (Cucurbita maxima) Fruit and Seeds Powders on Diabetic, Oxidative and Pancreatic Status in Rats

2019 ◽  
pp. 1-9
Author(s):  
Alyae, M. S. Gabal
Keyword(s):  
Blood Glucose ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Albino Rats ◽  
Cucurbita Maxima ◽  
Glucose Levels ◽  
Group I ◽  
Diabetic Control Group ◽  
Increased Risk

Background: Diabetes mellitus is the one of the most common endocrine diseases that is characterized by hyperglycemia, altered metabolism with an increased risk of much complications. Besides drugs classically used for the treatment of diabetes several species of plants have been described as having a hypoglycemic activity with decreased side effects. Aim of the Work: This work aimed to investigate the possible anti-diabetic effect of oral administration of pumpkin (Cucurbita maxima) fruit flesh and seeds powders on Streptozotocin induced diabetic rats via studying blood glucose levels, oxidative biomarkers as well as islets of Langerhans structure changes. Materials and Methods: 60 adult albino rats of Sprague-Dawely strains (200±5 gm) were classified into five groups of ten animals each except diabetic control group was composed of twenty rats as follow Group I: healthy control; Group II: diabetic control ,Group III, IV and V: diabetic rats received 2 g pumpkin fruit, seeds, fruit and seeds mixture powders respectively /kg body weight daily by oral intubation. Results: The results of present study showed that pumpkin powders caused significant improvements (P≤0.05) in blood glucose, insulin levels and glycated hemoglobin percent compared to diabetic control group. Also pumpkin powders improved antioxidants activities and healed Langerhans islets by increasing their number and size in comparison with diabetic control group. Conclusion: The present study showed that pumpkin powders may normalize the various biochemical and pancreatic tissues abnormalities resulted due to diabetes metabolic disorders and it is a source of potent anti-diabetic agent. The diabetic rats that were administered with the pumpkin fruit powder, exhibited the highest improvements.

scholarly journals Beneficial Hypoglycemic, Hypolipidemic Effects of Aerial Branches and Roots Extract of Eryngium caeruleum M. Bieb on Streptozotocin-Induced Diabetes Model in Rats

2021 ◽  
Author(s):  
Aryaneh Sardarbandeh ◽  
Mohammad Reza Delnavazi ◽  
Mohammad Sharifzadeh ◽  
Mohammad Sharifzadeh ◽  
Masoumeh Ghajarieh Sepanlou ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Blood Lipid ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Animal Blood ◽  
Hydroalcoholic Extract ◽  
Diabetic Control Group ◽  
Diabetes Model ◽  
Male Wistar Rats

Eryngium caeruleum (Apiacea) is native to the northern forests of Iran. The anti-diabetic effect of other species of the genus Eryngium has already been reported in previous studies. In this study, the anti-diabetic effect of this extract on animal blood lipid factors was investigated. Hydroalcoholic extract was obtained from different parts of the plant, including roots, leaves, and aerial branches with fruits were prepared by maceration with 70% ethanol. Oral acute toxicity of the extracts was assayed in different doses of 2000, 4000, and 8000 mg/kg in rats. To induce diabetes in the studied animals, 60-70 mg/kg of streptozotocin (STZ) was injected intraperitoneally (IP). For the purpose of this study, 72 male Wistar rats were randomly divided into different groups of normal, diabetic, and positive controls (metformin 500 mg/kg) as well as 9 diabetic groups that orally received 200, 400, and 800 mg/kg of extracts. The effects of the treatment with extracts for a 14-day period were investigated on weight, blood glucose, and lipid profile. By comparing the control groups with the groups of hydroalcoholic extracts of E. caeruleum showed that the most effective sample on weight gain and also on reducing blood glucose was the group receiving 800 mg/kg of the aerial branches extract (P < 0.01 and P < 0.001, respectively) after 14 days. As well, the most effective sample on lowering the blood lipid factors was the hydroalcoholic extract of the root of E. coareleum with a dose of 200 mg/kg, which showed a significant effect on lowering total cholesterol in diabetic rats compared to the diabetic controls (P < 0.05). Hydroalcoholic extract of leaves with 200 mg/kg also showed a better effect on lowering the LDL and VLDL levels compared to the diabetic control group (P < 0.001). The results of pancreatic histology in the samples showed that the extracts of the aerial branch and root (800 mg/kg) had significant effects on the regeneration of the islets of Langerhans compared to the diabetic control group. In conclusion, E. caeruleum could significantly improve glycemic and lipid profiles in diabetic rats.

scholarly journals EFFECT OF PITHECELLOBIUM DULCE BENTH LEAVES IN DEXAMETHASONE INDUCED DIABETIC RATS

2016 ◽  
Vol 8 (9) ◽  
pp. 317 ◽  
Author(s):  
Mule V. S. ◽  
Naikwade N. S. ◽  
Magdum C. S. ◽  
Jagtap V. A.
Keyword(s):  
Blood Glucose ◽  
Total Cholesterol ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Albino Rats ◽  
Glycogen Level ◽  
Standard Drug ◽  
Diabetic Control Group

<p><strong>Objective: </strong>The objective of the present study was to study the effect of <em>Pithecellobium dulce </em>Benth (<em>P. dulce</em>) leaves in dexamethasone-induced diabetic rats.</p><p><strong>Methods: </strong>The authenticated <em>P. dulce</em> leaves were collected from a local area of Sangli, Maharashtra. The leaves of the plant were extracted with water and ethanol by maceration and soxhelation respectively. Acute toxicity studies of the both extracts were performed using rat and according to OECD 425 guidelines. The dose of 200 mg/kg and 400 mg/kg was selected for further studies. The albino rats were divided into seven groups with five animals in each group. The diabetes was induced by dexamethasone (10 mg/kg, s. c.) and treated with extract and standard drug for 10 d. Then blood glucose, triglyceride, total cholesterol and glycogen level in liver, muscle and kidney were estimated according to standard procedures.</p><p><strong>Results: </strong>The study revealed that <em>P. dulce</em> at 200 mg/kg and 400 mg/kg showed significant (p ˂ 0.05) antidiabetic activity. All the extract treated groups showed a significant reduction in blood glucose level on 11<sup>th</sup> day when compared to diabetic control group. The significant increase in blood glucose, triglyceride, and total cholesterol level was observed in the diabetic control group when compared to normal control group. The liver and muscle glycogen level was decreased significantly (p ˂ 0.05) in the diabetic control group.<strong></strong></p><strong>Conclusion: </strong>It can be concluded that <em>P. dulce</em>aqueous and ethanolic extract at two different doses (200 mg/kg and 400 mg/kg) possesses antidiabetic and hypolipidemic activity.<p> </p>

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