Assessing the germinability of coastal Limonium minutum (Plumbaginaceae) under different temperature and salinity conditions: implications for its conservation

Background and aims – Limonium minutum is an endemic plant of the eastern Balearic Islands (Majorca and Minorca), where it grows in coastal rocky habitats. The effects of temperature and salinity on seed germination of Limonium minutum were evaluated in order to set a protocol for recovery of this species in this habitat.Material and methods – Experiments to determine the effect of temperature have been carried out at temperature conditions between 10 and 23°C. Tests to determine the effect of salinity have been evaluated at 18°C with concentrations of 0, 100, 200, 300, and 400 mM of MgCl2, MgSO4, NaCl, and Na2SO4.Key results – Maximum germination took place between 16 and 20°C. Based on the salinity tests, the highest germination values were obtained with distilled water. The use of saline solutions resulted in significant decreases in the germination percentage. However, in almost all treatments, seed germination was observed. The T50 increased at low temperatures and with increasing salt concentration.Conclusion – Limonium minutum has a wide germination temperature range and a high resistance to salinity. After being exposed to different saline solutions, once washed with distilled water, the seeds recover their full germination capacity; therefore, the effect of salts is an osmotic and non-toxic effect for this species. Sowing seeds in late August ensures that the species has an optimal chance to survive in coastal habitats.

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Effect of salinity and temperature on seed germination in Limonium cossonianum

Botany ◽

10.1139/cjb-2012-0157 ◽

2013 ◽

Vol 91 (1) ◽

pp. 12-16 ◽

Cited By ~ 19

Author(s):

Esther Giménez Luque ◽

Isabel C. Delgado Fernández ◽

Francisco Gómez Mercado

Keyword(s):

Seed Germination ◽

Germination Percentage ◽

Balearic Islands ◽

Distilled Water ◽

Endemic Plant ◽

Temperature Regimes ◽

Salinity Levels ◽

Mean Time ◽

Different Levels ◽

Ornamental Species

Limonium cossonianum Kuntze is an interesting endemic plant in the southeastern Iberian Peninsula and the Balearic Islands that can be used in wetland restoration and as an ornamental species in saline areas. Experiments were carried out to investigate seed germination responses in L. cossonianum at different levels of salinity (0, 100, 200, and 400 mmol/L NaCl) and under different temperature regimes (20/10, 25/15, 30/20, and 35/25 °C, day/night), both in a 14 h light – 10 h dark photoperiod. Seed germination in L. cossonianum was significantly affected by salinity levels, temperature, and the interaction of both parameters. Maximum germination was recorded in the lowest saline media (100 mmol/L NaCl) and distilled water (0 mmol/L NaCl) at 20/10 °C. No seeds germinated in concentrations above 200 mmol/L NaCl at the higher temperatures. The increase in salinity delayed the beginning and end of germination, reduced final germination percentage, and increased mean time to germination. The rate of germination decreased with an increase in salinity and temperature.

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The Effect of Priming Treatments on Germination and Seedling Performance of Purslane (Portulaca oleracea) Seed Lots

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Horticulture ◽

10.15835/buasvmcn-hort:0032 ◽

2017 ◽

Vol 74 (2) ◽

pp. 164

Author(s):

Eren OZDEN ◽

Ibrahim DEMIR

Keyword(s):

Seed Germination ◽

Relative Humidity ◽

Seedling Emergence ◽

Germination Percentage ◽

Portulaca Oleracea ◽

Distilled Water ◽

Climate Chamber ◽

Untreated Seed ◽

Percent Relative Humidity ◽

Purslane Seed

This study was conducted to test the effect of a priming combination on the seed germination percentage and seedling emergence performance of purslane under climate chamber and field conditions. Four purslane seed lots were treated according five different methods, which were T1: Seeds kept at a hundred percent relative humidity for four hours at 20 °C; T2: Seeds kept at a hundred percent relative humidity for four hours at 20 °C, and then soaked in distilled water for 8 hours at 5 °C; T3: Seeds kept at a hundred percent relative humidity for four hours at 20 °C, and then soaked in distilled water for 8 hours at 20 °C; T4: Seeds soaked in distilled water for 8 hours at 5 °C; T5: Seeds soaked in distilled water for 8 hours at 20 °C; and C: Control (untreated). Seed germination was calculated for 14 days at 20 °C, seedling emergence percentages were calculated in the climatically-controlled chamber for 21 days at 22 °C, and in the field for 35 days at 15-25 °C. The highest seed germination (94%) and seedling emergence in the climatically-controlled chamber (87%) and field (82%) were obtained from seeds that had been kept at a hundred percent relative humidity for four hours at 20 °C, then soaked in distilled water for eight hours at 5 °C. Results indicated that farm-priming, can be an efficient priming method in purslane seeds.

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The Effect of Temperature on Seed Germination and Seedling Growth of Two Invasive Plants in Rorippa

Biotechnology Journal International ◽

10.9734/bji/2020/v24i530116 ◽

2020 ◽

pp. 39-48

Author(s):

Qiuli Wang ◽

Bo Qu ◽

Juanjuan Mi ◽

Yufeng Xu ◽

Meini Shao

Keyword(s):

Seed Germination ◽

Invasive Plants ◽

Seedling Growth ◽

Petri Dish ◽

Germination Percentage ◽

Laboratory Culture ◽

Liaoning Province ◽

Effect Of Temperature ◽

Invasion Mechanism ◽

Root Shoot Ratio

Aims: By studying the response of seed germination and seedling growth of invasive plants, Rorippa amphibia and Rorippa sylvestris, to temperature, the influence of temperature on the invasive ability of two species of Rorippa were further analyzed, which provided a theoretical basis for revealing the diffusion and invasion mechanism of two invasive plants in Rorippa. Study Design: Seed germination and seedling growth test of two invasive plants in Rorippa at different temperature was studied by means of laboratory culture. The germination percentage, germination index, germination potential of the seeds and the total leaf number, root length, lateral root number, biomass and root shoot ratio of seedlings were determined. Place and Duration of Study: Seeds were collected from the Shenyang Agricultural University of Liaoning Province in July 2018. Experiments were done in the College of Bioscience and Biotechnology, and conducted in April 2019 for a month. Methodology: The petri dish method was used at the experiment of seed germination. The seedling growth experiment was carried out by pot sowing. Results: At the seedling stage.R. amphibia has the strongest tolerance at 30°C, while the R. sylvestris does at 35°C. The low temperature is more beneficial to the accumulations of the seedlings, R. amphibia is the most tolerant at 25°C, and R. sylvestris is at 30°C at the seedling stage. Conclusion: The response of the seeds and seedlings of the two species to temperature was basically the same. Higher temperature promoted seed germination and inhibited seedling growth, while lower temperature inhibited seed germination. The response of seeds and seedlings of R. sylvestris to high temperature makes it more invasive in the process of global warming.

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Sensitivity of Seed Germination to Temperature and Light in Helichrysum amorginum

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Horticulture ◽

10.15835/buasvmcn-hort:2020.0007 ◽

2020 ◽

Vol 77 (1) ◽

pp. 7

Author(s):

Sofia ARGYRIOU ◽

Panayiota PAPASTYLIANOU ◽

George STAVROPOULOS ◽

Angeliki KOUSTA ◽

Ioanna KAKABOUKI ◽

...

Keyword(s):

Seed Germination ◽

Seedling Survival ◽

Germination Percentage ◽

Effect Of Temperature ◽

Germination Time ◽

Randomized Design ◽

Perennial Shrub ◽

Mean Germination Time ◽

Lower Temperature ◽

Seed Propagation

Helichrysum amorginum is a perennial shrub, endemic on the Greek island of Amorgos and used for medicinal, cosmetic and ornamental purposes. Information concerning germination behavior and seedling survival is considered essential for the establishment and cultivation of wild species. The objective of this study was to investigate the effect of temperature and light on germination of Helichrysum amorginum seeds. A set of experiments was conducted under completely randomized design with four replications of 200 seeds at three different suboptimal germination temperatures (15, 20 and 25°C) in light and dark conditions for 10 days. Seed material was collected from two different fields of the island (Aegiali and Katapola). The germination performance was evaluated by final germination percentage and mean germination time. The effect of temperature on seed germination percentage and mean germination time was significant in all cases. The presence of light enhanced germination, especially at the optimum temperature of 20°C. In most cases the mean germination time increased at the lower temperature of 15°C. Seeds from Aegiali exhibited higher germination ability than the seed lot from Katapola. Knowledge of seed germination behaviour is of major importance in order to establish effective protocols for seed propagation in Helichrysum amorginum.

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Experimental seed germination for ex situ conservation of Mexican Podostemaceae

Botanical Sciences ◽

10.17129/botsci.2232 ◽

2019 ◽

Vol 97 (3) ◽

pp. 413 ◽

Cited By ~ 1

Author(s):

Valeria Flores-Enríquez ◽

Guillermo Castillo ◽

Margarita Collazo-Ortega

Keyword(s):

Seed Germination ◽

Storage Time ◽

Light Quality ◽

Germplasm Conservation ◽

Seed Storage ◽

Germination Percentage ◽

Seed Banks ◽

Ex Situ ◽

Effect Of Temperature ◽

Collection Site

Background: Podostemaceae are extremely susceptible to local extinction by habitat loss. Since ~70 % of the river systems in Mexico show some degree of water contamination, it is relevant to generate information about seed storage and germination behavior to design germplasm conservation strategies (e.g., ex situ seed banks) of Mexican podostemads.Hypotheses: Seed germination decreases as seed storage time increases. Further, light quality, temperature and collection site influence similarly the germination response of both species.Studied species: Marathrum foeniculaceum Humb. & Bonpl., Noveloa coulteriana (Tul.) C. PhilbrickStudy site and years of study: 13 different seeds collections (1996-2013), at four locations in the rivers Horcones and Arroyo del Rincon Jalisco, México. Methods: A germination chamber experiment was performed to evaluate the effect of temperature, light quality and storage time on the germination of both species.Results: Seeds lose viability after nine years of storage. In both species, the time to reach the highest Accumulated Germination Percentage (AGP) was faster in seeds of one-two yr compared to seeds of six-seven yr. N. coulteriana have significant differences in Final Germination Percentage (FGP) between collection rivers. Storage time affects AGP of N. coulteriana more than in M. foeniculaceum.Conclusions: Both species can form ex situ seed banks up to 8 yr age in paper bags storage. N. coulteriana is more susceptible to variation in storage conditions compared to M. foeniculaceum. Collection site affect seed germination after storing. Long-term conventional ex situ seed germination storage is not a viable strategy to conserve germplasm of Podostemaceae.

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Effects of temperature, gibberellic acid, and KNO3 treatments on seed germination of the wild plant Maesa japonica

Seed Science and Technology ◽

10.15258/sst.2020.48.1.09 ◽

2020 ◽

Vol 48 (1) ◽

pp. 65-72

Author(s):

Hyuk Joon Kwon ◽

So Lim Shin ◽

Yu-Ri Kim ◽

Soo-Young Kim

Keyword(s):

Seed Germination ◽

Gibberellic Acid ◽

Morphological Characteristics ◽

Germination Percentage ◽

Wild Plant ◽

Optimal Conditions ◽

Optimum Temperature ◽

Dark Cycle ◽

Germination Speed ◽

Effects Of Temperature

We investigated the morphological characteristics of Maesa japonica seeds and the optimal conditions for germination. The seeds were exalbuminous, and their length and width were 0.85 ± 0.09 mm and 0.77 ± 0.05 mm, respectively. We tested germination at 15, 20, 25, 30 and 35°C; the optimum temperature for germination was 30°C (64% germination). To test the effects of gibberellic acid (GA3) and KNO3 on germination, the seeds were soaked in solutions containing 0, 0.2, 0.5, 1.0, 1.5 or 2.0 g GA3 L-1 or 0, 0.05, 0.10, 0.20 or 0.50 g KNO3 L-1 before sowing for germination. KNO3 treatment increased germination percentage and speed. Soaking in GA3 concentrations over 1.0 g L-1 stimulated 100% germination after 13 days. A presoaking treatment that combined 1.0 g GA3 L-1 and 0.2 g KNO3 L-1 promoted germination speed and shortened the time in which half of the seeds germinated. Overall, we determined that the best method for germination of Maesa japonica seeds was pre-soaking in 1.0 g GA3 L-1 and 0.2 g KNO3 L-1 at 4°C in the dark for 24 hours, followed by incubation at 25–30°C in a 16-hour light, 8-hour dark cycle for 10 days.

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Temperature and Light Effects on Germination Behaviour of African Eggplant (Solanum aethiopicum L.) Seeds

Indian Journal of Agricultural Research ◽

10.18805/ijare.a-623 ◽

2021 ◽

Author(s):

H.M. Botey ◽

J.O. Ochuodho ◽

L. Ngode ◽

H. Dwamena ◽

I. Osei-Tutu

Keyword(s):

Seed Germination ◽

Seed Quality ◽

Light Exposure ◽

Germination Percentage ◽

Effect Of Temperature ◽

Maximum Temperature ◽

Percentage Germination ◽

Ambient Conditions ◽

Solanum Aethiopicum ◽

Germination Behaviour

Background: A preliminary study of the African eggplant seeds obtained from farmers sources recorded a wide variation in percentage germination under ambient conditions (25±2°C). The germination percentage ranged from 0% to 25%, while fresh seeds ranged between 53% and 87%. As temperature and light are important factors of seed germination, the current study investigated the effect of temperature on the germination pattern and the influence of light interaction with temperature on seed germination of African eggplant (Solanum aethiopicum L.) under controlled conditions. Methods: Seeds of two cultivars of African eggplant were subjected to constant and alternating temperatures and under three light exposure regimes. Seed quality was accessed by per cent germination, mean germination time, time to reach 50% germination, germination index and mean daily germination. Result: The highest percentage germination under constant temperatures was recorded at 25°C (76%) and 20°C (74%). The maximum temperature and light conditions required for maximum seed germination quality (76-95%) at the shortest time (4-5 days) was 30/20°C under alternating 8/16 hours light and dark.

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THE EFFECT OF CUPULE REMOVAL AND SEED HYDRATION ON THE GERMINATION OF TRIPSACUM DACTYLOIDES SEEDS

HortScience ◽

10.21273/hortsci.25.9.1118c ◽

1990 ◽

Vol 25 (9) ◽

pp. 1118c-1118

Author(s):

Jennifer Heisinger ◽

Rick Savage ◽

Dyremple Marsh ◽

Fred Hassien

Keyword(s):

Seed Germination ◽

Ethylene Glycol ◽

Germination Rate ◽

Germination Percentage ◽

Field Conditions ◽

Distilled Water ◽

Tripsacum Dactyloides ◽

Stand Establishment ◽

Normal Field ◽

Eastern Gamagrass

The germination percentage of eastern gamagrass under normal field conditions ranges from 5 to 10%. These rates are considered low and methods to improve stand establishment in this species are necessary, Studies were initiated to determine if cupule removal and seed hydration were effective in improving the germination percentage of eastern gamagrass. Seeds from which the cupules were removed were incubated for 30 days at 5° C in 100% ethylene glycol (EG). Control were seeds incubated in distilled water. At 12, 18, 24 and 30 days after the start of incubation, seeds from each group were removed and subjected to rapid warming at 25 - 27° C. Cupule removal significantly increased seed germination by as much as 55% over the control. Seeds with cupule removed and incubated in EG had a 65% germination rate,

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Evaluation of allelopathic effect of Carthamus Oxyacantha against wheat and maize seed germination

Letters in Applied NanoBioScience ◽

10.33263/lianbs91.814818 ◽

2020 ◽

Vol 9 (1) ◽

pp. 814-818

Keyword(s):

Seed Germination ◽

Dry Weight ◽

Germination Percentage ◽

Allelopathic Effect ◽

Distilled Water ◽

Maize Seed ◽

Fresh Weight ◽

Vigor Index ◽

Seedling Length ◽

Powder Extract

Effect of Carthamusoxyacantha powder aqueous extract on seed germination of Wheat and Maize were studied. Powder extract of 3g, 6g and 9g of concentration were soaked in distilled water for 12 Hrs, 24 Hrs and 36 Hrs of time. The seeds of Wheat and Maize were germinated in Petri dishes. Control was maintained by watering the seeds with distilled water. The highest values for germination percentage, shoot length, root length, fresh weight, dry weight, seedling length, vigor index and seedling weight vigor index were recorded in control of wheat the above parameter decreased with increasing concentration and timing. While in maize highest values for the above parameters were recorded in treatments as compared to control.

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Modeling the Effects of Temperature and Gibberellic Acid Concentration on Red Huckleberry Seed Germination

HortScience ◽

10.21273/hortsci.43.1.223 ◽

2008 ◽

Vol 43 (1) ◽

pp. 223-228 ◽

Cited By ~ 2

Author(s):

Omar A. Lopez ◽

Danny L. Barney ◽

Bahman Shafii ◽

William J. Price

Keyword(s):

Logistic Regression ◽

Seed Germination ◽

Gibberellic Acid ◽

Temperature Regime ◽

Potassium Salt ◽

Logistic Regression Model ◽

Germination Percentage ◽

Effects Of Temperature ◽

Lag Times ◽

Collection Sites

Low seed germination percentages have been reported for red huckleberry (Vaccinium parvifolium Smith). Attempts to improve germination percentages and the speed of germination for red huckleberry are described. Red huckleberry seeds from two collection sites were given gibberellic acid potassium salt (GA-K) treatments (0, 500, 1000, and 1500 mg·L−1) and were germinated under three temperature regimens [constant 22 °C, 22 °C day/5 °C night (22/5 °C), and 20 °C day/13 °C night (20/13 °C) with a 12-h photoperiod]. A logistic regression model was used to assess the effects of temperature regimens and GA-K treatments on the maximum cumulative germination percentages, rates of increase, and germination lag times. For seeds untreated with GA-K, the 20/13 °C temperature regime resulted in germination percentages ranging from 30% to 61% and lag times (i.e., time to reach one-half of the maximum cumulative germination percentage) of 29 to 35 d for the two accessions. In comparison, the 22/5 °C temperature regime produced germination percentages of 12% and 38% and lag times of 38 to 64 d. The 22 °C constant temperature produced germination percentages ≤1%. Maximum germination percentages of up to 75% were obtained with 1500 mg/L GA-K. Rates of germination were generally unaffected by GA-K treatments, and germination lag times were reduced by an average of 10 d when compared with without GA-K. Improved germination percentages and reduced lag times for red huckleberry seeds were obtained by using a 20/13 °C temperature regime and 1000 to 1500 mg·L −1 GA-K.

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