scholarly journals Karakteristik Organoleptik dari tempe modifikasi dengan kacang kedelai hitam (Glycine max (L) Merrit)

2021 ◽  
Vol 2 (2) ◽  
pp. 135-140
Author(s):  
Alfi Rahmawan ◽  
Ihsan Jabran Mumtaz ◽  
Rera Haiefinah ◽  
Lulu Zakiyah Hasna ◽  
Elisabeth Michaela Pareira ◽  
...  

Tempeh is a fermented food from soybean seeds using the fungus Rhizopus oligosporus. Tempeh is usually made from ordinary fermented soybeans. However, there are various types of beans besides ordinary soybeans, one of which is black soybeans. Food product innovation is closely related to consumer acceptance. Acceptance can be measured using organoleptic test. Organoleptic testing is an important component in the process of making food product innovations. In this study, tempeh was made with a mixture of soybeans and black soybeans in a ratio of 50:50. The resulting tempeh was then tested organoleptically including the parameters of color, texture, aroma, and overall and compared with commercial tempeh on 45 panelists. Based on this research, it is known that the overall parameters, namely color, texture, aroma, and overall in soybean tempeh and 50:50 black soybeans have lower acceptability than commercial tempeh so that further development of these products is needed so that consumer acceptance is higher increase.

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Haruka Sugiura ◽  
Ayumi Nagase ◽  
Sayoko Oiki ◽  
Bunzo Mikami ◽  
Daisuke Watanabe ◽  
...  

Abstract Saprophytic bacteria and plants compete for limited nutrient sources. Bacillus subtilis grows well on steamed soybeans Glycine max to produce the fermented food, natto. Here we focus on bacterial responses in conflict between B. subtilis and G. max. B. subtilis cells maintained high growth rates specifically on non-germinating, dead soybean seeds. On the other hand, viable soybean seeds with germinating capability attenuated the initial growth of B. subtilis. Thus, B. subtilis cells may trigger saprophytic growth in response to the physiological status of G. max. Scanning electron microscope observation indicated that B. subtilis cells on steamed soybeans undergo morphological changes to form apertures, demonstrating cell remodeling during saprophytic growth. Further, transcriptomic analysis of B. subtilis revealed upregulation of the gene cluster, yesOPQR, in colonies growing on steamed soybeans. Recombinant YesO protein, a putative, solute-binding protein for the ATP-binding cassette transporter system, exhibited an affinity for pectin-derived oligosaccharide from plant cell wall. The crystal structure of YesO, in complex with the pectin oligosaccharide, was determined at 1.58 Å resolution. This study expands our knowledge of defensive and offensive strategies in interspecies competition, which may be promising targets for crop protection and fermented food production.


Agronomy ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1105
Author(s):  
Małgorzata Świątkiewicz ◽  
Kamil Witaszek ◽  
Ewa Sosin ◽  
Krzysztof Pilarski ◽  
Beata Szymczyk ◽  
...  

Post-extraction soybean (Glycine max (L.) Merr.) meal is widely used as a basic protein feed for farm animals, especially poultry and pigs. Products made from unmodified soybean seeds are an alternative to imported GMO soybean meal. The aim of the study was to develop feed products from popular European varieties of genetically unmodified soybeans, which can be produced on small and medium-sized farms, and to assess their nutritional value and safety to livestock. The research was conducted on the seeds of three soybean varieties and two types of feed products resulting from thermobaric treatment (extrudate) and oil pressing (soybean press cake). The mould and yeast contamination of domestic seeds was negligible. The thermobaric and pressing treatments lowered the content of fungi by 97%. The products were considered free from mycotoxins. In comparison with full-fat soybean seeds, the protein content in the products was up to 19% higher, and 92% of the total lysine remained available. The products had lower content of antinutritional ingredients (trypsin inhibitors) and the urease activity was reduced by 52–59% and 99%, respectively. The experiment showed that the European genetically unmodified soybean feed products were characterised by good quality, mycotoxicological purity and high nutritional value for farm animals.


2021 ◽  
Vol 11 ◽  
Author(s):  
Mu-Chien Lai ◽  
Zheng-Yuan Lai ◽  
Li-Hsin Jhan ◽  
Ya-Syuan Lai ◽  
Chung-Feng Kao

Soybean [Glycine max (L.) Merr.] is one of the most important legume crops abundant in edible protein and oil in the world. In recent years there has been increasingly more drastic weather caused by climate change, with flooding, drought, and unevenly distributed rainfall gradually increasing in terms of the frequency and intensity worldwide. Severe flooding has caused extensive losses to soybean production and there is an urgent need to breed strong soybean seeds with high flooding tolerance. The present study demonstrates bioinformatics big data mining and integration, meta-analysis, gene mapping, gene prioritization, and systems biology for identifying prioritized genes of flooding tolerance in soybean. A total of 83 flooding tolerance genes (FTgenes), according to the appropriate cut-off point, were prioritized from 36,705 test genes collected from multidimensional genomic features linking to soybean flooding tolerance. Several validation results using independent samples from SoyNet, genome-wide association study, SoyBase, GO database, and transcriptome databases all exhibited excellent agreement, suggesting these 83 FTgenes were significantly superior to others. These results provide valuable information and contribution to research on the varieties selection of soybean.


2017 ◽  
Vol 9 (5) ◽  
pp. 792-802 ◽  
Author(s):  
Hai-jun Wu ◽  
Jun-cai Deng ◽  
Cai-qiong Yang ◽  
Jing Zhang ◽  
Qing Zhang ◽  
...  

Twelve isoflavones and eight anthocyanins were quantified in black soybean seeds by HPLC-MS analyses; the coupled OPLS-DA multivariate statistical analysis helped us to determine their geographical origin.


Genome ◽  
1987 ◽  
Vol 29 (5) ◽  
pp. 713-717 ◽  
Author(s):  
Joe-Yuan H. Doong ◽  
Yun-Tzu Kiang

A six-band electrophoretic zymogram for aconitase was observed in mature soybean seeds. Among 750 Glycine max (L.) Merr. strains and cultivars examined, 10 zymogram types were identified. Genetic studies showed that the second band (Rf's of 0.35 or 0.42) and the fourth band (Rf's of 0.61, 0.63, or 0.69) in the zymograms were the products of two unlinked loci, Aco2 and Aco4, with two and three codominant alleles, respectively. Heterozygotes displayed both parental band sets without any intermediate band, indicating a monomeric structure for aconitase. The most common alleles at the two loci were Aco2-b (0.793) and Aco4-c (0.818). Linkage analyses revealed that both Aco2 and Aco4 loci are inherited independently of four other biochemical loci (Adh1, Am3, Ap, Dial). Key words: aconitase, isozymes, electrophoresis, gene locus, soybean, Glycine max.


Plant Disease ◽  
1997 ◽  
Vol 81 (6) ◽  
pp. 693-693 ◽  
Author(s):  
K. W. Roy ◽  
S. Ratnayake

Mature cowpea (Vigna unguiculata (L.) Walp.) pods with scattered, irregular black spots, similar in appearance to those found on mature soybean (Glycine max (L.) Merr.) pods infected with Phomopsis longicolla T. W. Hobbs, were observed in three fields in Oktibbeha County, MS, in September 1994. In a delayed harvest sample of pods and seeds from one field, a fungus identified as P. longicolla (1) was isolated from more than 60% of surface-disinfested, excised pod disks and from 42% of surface-disinfested seeds. Average frequencies of isolation from seeds harvested at the normal time from the other two fields were 27 and 9%. When surface-disinfested soybean seeds (cv. Avery) were placed along the margins of P. longicolla colonies on potato dextrose agar, the radicals from most germinating seeds became necrotic and many seeds did not germinate. Koch's postulates were completed by reisolating the fungus from diseased tissue. Reference: (1) T. W. Hobbs et al. Mycologia 77:535, 1985.


2010 ◽  
Vol 45 (12) ◽  
pp. 2524-2530 ◽  
Author(s):  
Antonio Jiménez-Escrig ◽  
Myrian-Thereza Serra ◽  
Pilar Rupérez

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