scholarly journals Arsenic Induced Vascular Endothelial Dysfunction Prevented by Antioxidants in Fetal kidneys of Albino Mice

2021 ◽  
Vol 31 (01) ◽  
pp. 18-23
Author(s):  
Fariha Qureshi ◽  
Muhammad Tahir
Keyword(s):  
Blood Vessels ◽  
Arsenic Exposure ◽  
Vascular Lesions ◽  
Control Group ◽  
Sodium Arsenate ◽  
Entire Pregnancy ◽  
Chronic Arsenic Exposure ◽  
Group A ◽  
Albino Mice ◽  
Group B

Background &Objectives: Cardiovascular diseases and hypertension has a significant correlation with chronic arsenic exposure through drinking water. This study was designed to investigate the prevention of sodium arsenate induced vascular disorders by Vitamins C& E in fetal renal blood vessels of albino mice. Materials & Methods: Gravid albino mice of BALB/c strain (twenty four) were randomly divided into 4 groups having 6 animals each. Control group A was inoculated with 0.1ml/kg/day distilled water I/P for 18 days.  Animals of groups B, C & D were given a single I/P injection of sodium arsenate 35mg/kg on 8th GD, whereas groups C and D were also injected with Vitamin C,  9 mg/kg/day and vitamin E 15 mg/kg/day by I/P route, beginning from 8th GD and continued for the entire pregnancy period. On 18th day of gestation fetal kidneys were extracted. Histological examination of renal blood vessels was performed for any discernable congestion, endothelial disruption and hyalinization and frequency of changes were expressed as percentages.  Results: In group (B) sodium arsenate induction resulted in congestion of blood vessels, hemorrhages in glomerular capillaries and thickening of endothelial walls. The addition of Vitamins C and E in groups C & D respectively had reduced the congestion and endothelial thickening. Mean score of histological changes was statistically significant. Conclusions: The results showed the antioxidant prospective of Vitamins C and E in combating against the vascular lesions induced by sodium arsenate.  

Chloroquine Induced Hepatotoxicity in Male Albino Mice: RCT

2021 ◽  
Vol 15 (10) ◽  
pp. 3070-3071
Author(s):  
Sumbal Khalid ◽  
Hamid Javaid Qureshi ◽  
Talha Laique
Keyword(s):  
Body Weight ◽  
Oral Dose ◽  
Single Oral Dose ◽  
Controlled Study ◽  
Control Group ◽  
Trial Methodology ◽  
Clinical Trial Methodology ◽  
Group A ◽  
Albino Mice ◽  
Group B

Many drugs have been found to induce hepatotoxicity and acute liver failure. Chloroquine is one of those drugs, which can induce hepatotoxicity when it is given at higher dose Purpose: To find the effect of chloroquine on liver function tests (LFTs) Study Design: Randomized clinical trial Methodology: Sixty male albino mice were taken into this randomized controlled study. Those were divided into two groups of 30 each. Group A was the control group while group B mice were given single oral dose of 970 mg/kg of body weight of chloroquine on 9th day of experiment. Terminal intracardiac blood sample was obtained on 17th day of experiment Statistical analysis: SPSS version 23 was used for data analysis Results: When results of group B were compared with those of group A, they depicted highly significant (p=0.000) rise in serum ALP. Serum albumin decreased significantly (p= 0.007). Serum AST increased significantly (p=0.005). Serum ALT, however, did not rise significantly (p=0.285) in group B. Similarly, serum total proteins did not decrease significantly ( p=0.530) in group B Conclusion: It was concluded that chloroquine induced mild hepatotoxicity in male albino mice when a single oral dose of 970 mg/kg of body weight of it is given Key Words: Chloroquine, Hepatotoxicity and Alkaline Phosphatase.

scholarly journals Histological Changes in Kidney and Spleen of Albino Mice Treated with Brake Pad Particles

2019 ◽  
pp. 2540-2549
Author(s):  
Nada Abdulrahman F. Al-Easawi ◽  
Hajer Qais Ghaidan ◽  
Shahad Mansour Salman
Keyword(s):  
Exposure Dose ◽  
Control Group ◽  
Continuous Exposure ◽  
Brake Pad ◽  
Amyloid Deposits ◽  
Vascular Congestion ◽  
Total Suspended Particles ◽  
Group A ◽  
Albino Mice ◽  
Group B

In this study, the consequences of treatment with brake pad particles on kidney and spleen were evaluatedthrough microscopic anatomy sections for 60 male albino mice. The animals were divided into six groups, the first three groups (A,B,C)  were exposed to brake pad particles depending on periods of exposure (4, 8, and 12weeks, respectively), while the other three groups were control groups,designated asF, which were exposed to laboratory fresh air only. A special locally-designed inhalation chamber was used to expose the animals. The exposure dose to brake pad particles (total suspended particles) was 2.228 µg/m³ for 30 min/day, 5 days/week,4, 8 and 12 weeks.The statistical analysis showed that the weights of organs for both kidney and spleen of treated mice had highly significant differences (P< 0.01) compared with control groups.The histological sections examination of the kidney, when compared with the control group, showed subcapsular tubular vascular degeneration and mild cortical focal hemorrhage in group A that was exposedto brake pads particlesfor 4 weeks. In group B, the exposure for 8 weeks resulted in cortical vascular degeneration, cortico-medullary vascular congestion, focal interstitial nephritis, and thickening of interstitial tissue.Other effects included marked dilation of collecting ducts with tubular vascular degeneration, necrosis, glomerular degeneration and deterioration, and cast formation. Group C, exposed for 12 weeks , showed similar changes of the kidney to those of group B. As related to spleen sections,they demonstrated mild subcapsular vascular degeneration of lymphocytes in group A,while the exposure in group Bresulted in moderate amyloid deposits with hemosiderosis, along withmild subcapsular vascular degeneration of lymphocyteswithin the red pulp. In group C, which was treated for 12 months, severe splenomegaly with advanced secondary splenic amyloidosis was also observed within the red pulp.It was characterized by marked deposited acellular and amorphous pinkish homogenous material within red pulp.The continuous exposure to brake pad particles causes harmful effectson the tissues ofessential body organs in human health.These particles should be thought-about as a sort of additional air pollutants in several cities of Iraqin the future.   

Chloroquine Induced Oxidative Stress in Male Albino Mice: RCT

2021 ◽  
Vol 15 (11) ◽  
pp. 3214-3215
Author(s):  
Sumbal Khalid ◽  
Hamid Javaid Qureshi ◽  
Muhammad Sohail Aslam ◽  
Talha Laique
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Controlled Study ◽  
Control Group ◽  
Trial Methodology ◽  
Clinical Trial Methodology ◽  
Group A ◽  
Albino Mice ◽  
Group B ◽  
As Stress

Many drugs have been found to induce oxidative stress. Oxidative stress is responsible for a large number of diseases. Chloroquine is one of the drugs, which can induce oxidative stress, when it is given at higher dose. Purpose: To find the effect of chloroquine as stress inducer on albino mice. Study Design: Randomized clinical trial. Methodology: Sixty male albino mice were taken into this randomized controlled study. Those were divided into two groups of 30 each. Group A was the control group while group B mice were given single oral dose of 970 mg/kg of body weight of chloroquine on 9th day of experiment. Terminal intracardiac blood sample was obtained on 17th day of experiment. Statistical analysis: SPSS version 23 was used for data analysis. Results: When results of group B were compared with those of group A, there was highly significant (p= 0.000) rise in serum malondialdehyde level and highly significant (p= 0.000) decrease in serum glutathione peroxidase level. Conclusion: It was concluded that Chloroquine induces oxidative stress when it is given at the dose of 970 mg/kg of body weight in mice. Keywords: Chloroquine, Oxidative Stress and Malondialdehyde.

Blood Viscosity and Platelet Aggregation in Male and Female Patients with Transient Ischaemic Attacks (TIA)

1979 ◽  
Author(s):  
G. Palareti ◽  
M. Poggi ◽  
G. Fortunato ◽  
S. Coccheri
Keyword(s):  
Platelet Aggregation ◽  
Blood Viscosity ◽  
Indirect Evidence ◽  
Vascular Lesions ◽  
Transient Ischaemic Attacks ◽  
Group A ◽  
Circulating Platelet Aggregates ◽  
Male Patients ◽  
Group B ◽  
Almost All

A series of 40 patients with TIA (25 males and 15 females) was thoroughly investigated by means of angiography and computerized tomography, and divided into a group (A) of 15 “sine materia”, and a group (B) of 25 with direct or indirect evidence of vascular occlusive or stenotic changes. Blood viscosity at 230 sec-1 37° was cp 4.2 ± 0.3 in the controls, cp 4.7 ± 0.7 in all patients (p < 0.05) cp 4.98 ± 0.7 in all male patients (p < 0.01 versus male controls), and cp 4.75 ± 0.8 in group B (p < 0.02). Haematocrit and Fibrinogen were also significantly increased in all male patients and in group B. Circulating platelet aggregates (CPA) were increased in 40% of the patients. Almost all patients with elevated CPA were males, with a slight prevalence in group B. Changes in blood viscosity parameters and in platelet aggregation in TIA patients were therefore related both to evidence of vascular lesions, and to sex, since they were found to prevail in male patients of both groups.

Clinical effect of Suvarna Bindu Prashan

2017 ◽  
Vol 2 (3) ◽  
Author(s):  
Patil Aniket ◽  
Dindore Pallavi ◽  
Arbar Aziz ◽  
Kadam Avinash ◽  
Saroch Vikas
Keyword(s):  
Physical Health ◽  
Eating Habits ◽  
Control Group ◽  
Inform Consent ◽  
Significant Difference ◽  
Group A ◽  
Group B ◽  
Immune Booster ◽  
Mental And Physical Health ◽  
Apparently Healthy

The quest for excellence in mental and physical health is not new. We find various references and formulations in Ayurvedic classics meant for promoting mental and physical health of a child. Suvarna Prashan is one of the formulations explained in age old Ayurvedic classic Kashyap Samhita. This formulation is very widely used now days as a memory and immune booster for children. But there is very little systematic documented study which can be used to evaluate the efficacy of the formulation. Suvarna Bhasma was prepared in Ayurved Rasayani Pharmacy, Pune. Madhu and Ghrita were collected from KLE Ayurveda Pharmacy, Belgaum. Suvarna Bindu Prashan was prepared in KLE Ayurved pharmacy, Belgaum. It contains Suvarna Bhasma, Ghrita and Madhu. Twenty apparently healthy male and female children with age group of three to four years were ready to sign inform consent form were selected into two groups each. Subjects in Group A received Suvarna Bindu Prashan where as Group B (Control group) did not receive any treatment. Both the groups were observed for six months. Children in Suvarna Bindu Prashan group showed significant reduction in the scores of eating habits, behavior, mood, temperament and scores of event of illness. However there was no significant difference in the score of sleeping habit. There was significant increase in IQ percentage.

scholarly journals Microecological preparation combined with an modified low-carbon diet improves glucolipid metabolism and cardiovascular complication in obese patients

2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Jianguo Liu ◽  
Liehui Xiao ◽  
Hezhongrong Nie ◽  
Yong Pan ◽  
Yan Liu ◽  
...  
Keyword(s):  
Intestinal Barrier ◽  
Intestinal Barrier Function ◽  
Control Group ◽  
Obese Patients ◽  
Carotid Atherosclerotic Plaque ◽  
Low Carbon ◽  
Balanced Diet ◽  
Group A ◽  
Group B ◽  
Control Group Group

Abstract Objective To investigate the impact of microecological preparation combined with modified low-carbon diet on the glucolipid metabolism and cardiovascular complication in obese patients. Methods From August 2017 to July 2020, 66 obese patients were recruited, and administrated with an modified low-carbon diet with (group A) or without (Group B) microecology preparation and a balanced diet in control group (group C) for 6 months. Meanwhile, 20 volunteers administrated with a balanced diet were recruited as the healthy control group (group D). Results After 6-month intervention, obese subjects in group A and B showed significant improvement of body and liver fat mass, reduction of serum lipid levels, intestinal barrier function markers, insulin resistance index (IRI), high blood pressure (HBP) and carotid intima thickness, as compared with subjects in group C. More importantly, subjects in group A had better improvement of vascular endothelial elasticity and intimal thickness than subjects in group B. However, these intervention had no effect on carotid atherosclerotic plaque. Conclusion Administration of microecological preparation combined with modified low-carbon diet had better improvement of intestinal barrier function, glucose and lipid metabolism, and cardiovascular complications than low-carbon diet in obese patients, but the effect of a simple low-carb diet on carotid atherosclerotic plaque need to be further addressed.

scholarly journals Effects of different thermal insulation methods on the nasopharyngeal temperature in patients undergoing laparoscopic hysterectomy: a prospective randomized controlled trial

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Guanyu Yang ◽  
Zefei Zhu ◽  
Hongyu Zheng ◽  
Shifeng He ◽  
Wanyue Zhang ◽  
...  
Keyword(s):  
Thermal Insulation ◽  
Primary Outcome ◽  
Controlled Trial ◽  
Laparoscopic Hysterectomy ◽  
Control Group ◽  
Prospective Randomized Controlled Trial ◽  
Warming Blanket ◽  
Group A ◽  
Insulation Effect ◽  
Group B

Abstract Background This study explored the comparison of the thermal insulation effect of incubator to infusion thermometer in laparoscopic hysterectomy. Methods We assigned 75 patients enrolled in the study randomly to three groups: Group A: Used warming blanket; group B: Used warming blanket and infusion thermometer; group C: Used warming blanket and incubator. The nasopharyngeal temperature at different time points during the operation served as the primary outcome. Results The nasopharyngeal temperature of the infusion heating group was significantly higher than that of the incubator group 60 min from the beginning of surgery (T3): 36.10 ± 0.20 vs 35.81 ± 0.20 (P<0.001)90 min from the beginning of surgery (T4): 36.35 ± 0.20 vs 35.85 ± 0.17 (P<0.001). Besides, the nasopharyngeal temperature of the incubator group was significantly higher compared to that of the control group 60 min from the beginning of surgery (T3): 35.81 ± 0.20 vs 35.62 ± 0.18 (P<0.001); 90 min from the beginning of surgery (T4): 35.85 ± 0.17 vs 35.60 ± 0.17 (P<0.001). Regarding the wake-up time, that of the control group was significantly higher compared to the infusion heating group: 24 ± 4 vs 21 ± 4 (P = 0.004) and the incubator group: 24 ± 4 vs 22 ± 4 (P = 0.035). Conclusion Warming blanket (38 °C) combined infusion thermometer (37 °C) provides better perioperative thermal insulation. Hospitals without an infusion thermometer can opt for an incubator as a substitute. Trial registration This trial was registered with ChiCTR2000039162, 20 October 2020.

scholarly journals The Bone Buttress Theory: The Effect of the Mechanical Loading of Bone on the Osseointegration of Dental Implants

Biology ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 12
Author(s):  
David Chavarri-Prado ◽  
Aritza Brizuela-Velasco ◽  
Ángel Álvarez-Arenal ◽  
Markel Dieguez-Pereira ◽  
Esteban Pérez-Pevida ◽  
...  
Keyword(s):  
Dental Implants ◽  
Mechanical Loading ◽  
Bone Growth ◽  
Bone Matrix ◽  
Test Group ◽  
Control Group ◽  
Group A ◽  
Bone To Implant Contact ◽  
The Stability ◽  
Group B

Objectives: To determine the effect of mechanical loading of bone on the stability and histomorphometric variables of the osseointegration of dental implants using an experimental test in an animal model. Materials and Methods: A total of 4 human implants were placed in both tibiae of 10 New Zealand rabbits (n = 40). A 6-week osseointegration was considered, and the rabbits were randomly assigned to two groups: Group A (Test group) included 5 rabbits that ran on a treadmill for 20 min daily during the osseointegration period; Group B (Controls) included the other 5 that were housed conventionally. The monitored variables were related to the primary and secondary stability of the dental implants (implant stability quotient—ISQ), vertical bone growth, bone to implant contact (BIC), area of regenerated bone and the percentage of immature matrix. Results: The results of the study show a greater vertical bone growth (Group A 1.26 ± 0.48 mm, Group B 0.32 ± 0.47 mm, p < 0.001), higher ISQ values (Group A 11.25 ± 6.10 ISQ, 15.73%; Group B 5.80 ± 5.97 ISQ, 7.99%, p = 0.006) and a higher BIC (Group A 19.37%, Group B 23.60%, p = 0.0058) for implants in the test group, with statistically significant differences. A higher percentage of immature bone matrix was observed for implants in the control group (20.68 ± 9.53) than those in the test group (15.38 ± 8.84) (p = 0.108). A larger area of regenerated bone was also observed for the test implants (Group A 280.50 ± 125.40 mm2, Group B 228.00 ± 141.40 mm2), but it was not statistically significant (p = 0.121). Conclusions: The mechanical loading of bone improves the stability and the histomorphometric variables of the osseointegration of dental implants.

Glycine and L-Arginine supplementation ameliorates gastro-duodenal toxicity in a rat model of NSAID (Diclofenac)-gastroenteropathy via inhibition of oxidative stress

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Akinleye Stephen Akinrinde ◽  
Halimot Olawalarami Hameed
Keyword(s):  
Oxidative Stress ◽  
Total Protein ◽  
Therapeutic Index ◽  
Control Treatment ◽  
Control Group ◽  
Protective Effects ◽  
Serum Total Protein ◽  
Significant Amelioration ◽  
Group A ◽  
Group B

Abstract Objectives This study examined the possible protective roles of exogenous glycine (Gly) and L-Arginine (l-Arg) against Diclofenac (DIC)-induced gastro-duodenal damage in rats. Methods Rats were divided into Group A (control), Group B (DIC group) and Groups C–F which were pre-treated for five days with Gly1 (250 mg/kg), Gly2 (500 mg/kg), l-Arg1 (200 mg/kg) and l-Arg2 (400 mg/kg), respectively, before co-treatment with DIC for another three days. Hematological, biochemical and histopathological analyses were then carried out. Results DIC produced significant (p<0.05) reduction in PCV (13.82%), Hb (46.58%), RBC (30.53%), serum total protein (32.72%), albumin (28.44%) and globulin (38.01%) along with significant (p<0.05) elevation of serum MPO activity (83.30%), when compared with control. In addition, DIC increased gastric H2O2 and MDA levels by 33.93 and 48.59%, respectively, while the duodenal levels of the same parameters increased by 19.43 and 85.56%, respectively. Moreover, SOD, GPx and GST activities in the DIC group were significantly (p<0.05) reduced in the stomach (21.12, 24.35 and 51.28%, respectively) and duodenum (30.59, 16.35 and 37.90%, respectively), compared to control. Treatment with Gly and l-Arg resulted in significant amelioration of the DIC-induced alterations although l-Arg produced better amelioration of RBC (29.78%), total protein (10.12%), albumin (9.93%) and MPO (65.01%), compared to the DIC group. The protective effects of both amino acids against oxidative stress parameters and histological lesions were largely similar. Conclusions The data from this study suggest that Gly or l-Arg prevented DIC-induced gastro-duodenal toxicity and might, therefore be useful in improving the therapeutic index of DIC.

scholarly journals Clinical and Microbiological Evaluation of Local Doxycycline and Antimicrobial Photodynamic Therapy during Supportive Periodontal Therapy: A Randomized Clinical Trial

Antibiotics ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 277
Author(s):  
Raluca Cosgarea ◽  
Sigrun Eick ◽  
Ionela Batori-Andronescu ◽  
Søren Jepsen ◽  
Nicole B. Arweiler ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Bacterial Species ◽  
Periodontal Therapy ◽  
Local Drug Delivery ◽  
Control Group ◽  
Periodontal Pathogens ◽  
Supportive Periodontal Therapy ◽  
Group A ◽  
Group B ◽  
Attachment Level

The aim of this study was to evaluate the clinical and microbiological effects of subgingival instrumentation (SI) alone or combined with either local drug delivery (LDD) or photodynamic therapy (PDT) in persistent/recurrent pockets in patients enrolled in supportive periodontal therapy (SPT). A total of 105 patients enrolled in SPT were randomly treated as follows: group A (n = 35): SI +PDT and 7 days later 2nd PDT; group B (n = 35): SI+LDD; group C (n = 35): SI (control). Prior intervention, at 3 and 6 months after therapy, probing pocket depths, clinical attachment level, number of treated sites with bleeding on probing (n BOP), full mouth plaque and bleeding scores (gingival bleeding index, %BOP) were recorded. At the same time points, 8 periodontopathogens were quantitatively determined. All three treatments resulted in statistically significant improvements (p < 0.05) of all clinical parameters without statistically significant intergroup differences (p > 0.05). Several bacterial species were reduced in both test groups, with statistically significantly higher reductions for LDD compared to PDT and the control group. In conclusion, the present data indicate that: (a) In periodontal patients enrolled in SPT, treatment of persistent/recurrent pockets with SI alone or combined with either PDT or LDD may lead to comparable clinical improvements and (b) the adjunctive use of LDD appears to provide better microbiological improvements for some periodontal pathogens than SI alone or combined with PDT.

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