Determination of Total Phenolic, Total Flavonoid and Antioxidant Activity of Karamunting Leaves (Rhodomyrtus tomentosa (Aiton) Hassk) Extract and Fraction

Karamunting (Rhodomyrtus tomentosa (Aiton) Hassk) is a family of Myrtaceae which has been used by the community as traditional medicine. The purpose of this research is determine total phenolic and total flavonoid level and test antioxidant activity of Karamunting Leaves. Determination of total phenolic using gallic acid standard by the Folin Ciocalteu method. The results obtained total phenolic in water fraction of 99 mgGAE/g, ethanol extract 94,1 mgGAE/g, ethyl acetate fraction 83,3 mgGAE/g and n-hexane fraction 41,4 mgGAE/g. Determination of total flavonoid using standard quersetin with AlCL3 complex formation method, the results obtained total flavonoid in water fraction of 156,8 mgQE/g, ethanol extract 192,6 mgQE/g, ethyl acetate fraction 89,4 mgQE/g and n-hexane fraction 31,3 mgQE/g. Determination of antioxidant activity was determined by DPPH radical capture test (1,1-diphenyl-2-picrylhydrazyl), the results obtained in water fraction with IC50 value 15,02 µg/mL, ethanol extract 14,06 µg/mL, ethyl acetate fraction 14,48 µg/mL with very strong activity of antioxidant, whereas in the n-hexane fraction was categorized as weak antioxidant activity with an IC50 value of 264,02 µg/mL.

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THE ANTIOXIDANT ACTIVITY AND PHYTOCHEMICAL SCREENING OF ETHANOL EXTRACT, FRACTIONS OF WATER, ETHYL ACETATE AND n-HEXANE FROM MISTLETOE TEA (SCURRULA ATROPURPUREABL. DANS)

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i2.15724 ◽

2017 ◽

Vol 10 (2) ◽

pp. 343 ◽

Cited By ~ 4

Author(s):

Resmi Mustarichie

Keyword(s):

Antioxidant Activity ◽

Ascorbic Acid ◽

Ethyl Acetate ◽

Antioxidant Activities ◽

Ethanol Extract ◽

Ethyl Acetate Fraction ◽

Hexane Fraction ◽

Phytochemical Screening ◽

Water Fraction ◽

Ic50 Values

Objective: The aim of this study is to investigate antioxidant activity and phytochemical screening of ethanol extract, fractions of water, ethyl acetate, and n-hexane from mistletoe tea (Scurrula atropurpurea Bl. Dans).Methods: Simplicia extracted using soxhlet equipment with 96% ethanol. Fractionation was conducted using liquid-liquid extraction using a solvent of water, ethyl acetate and n-hexane. Screening of phytochemical and antioxidant activity was performed against these fractions. Antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl method using ultraviolet-visible spectrophotometry with ascorbic acid as standard. Phytochemical screening was conducted based on the method of Farnsworth.Results: The IC50 values of ethanol extract, water fraction, fraction of ethyl acetate, and n-hexane fraction were 21.92 ppm, 89.57 ppm, 14.08 ppm, and 162.09 ppm, respectively, whereas for ascorbic acid was 4.41 ppm. The ethanol extract and ethyl acetate fraction contained compounds were the same group, polyphenolic, tannins, flavonoids, monoterpenoid, steroids, triterpenoids, and quinones. Fraction of water contained compounds such as polyphenolic group, flavonoids, monoterpenoids, sesquiterpenoids, steroids, and triterpenoids. n-hexane fraction compounds contained steroids and triterpenoids.Conclusion: The ethanol extract, water fraction, ethyl acetate fraction, and n-hexane fraction showed antioxidant activities. The ethanol extract, fractions of water, and ethyl acetate fraction contained flavonoids and polyphenolic potential as antioxidants.Keywords: Mistletoe tea, Scurrula atropurpurea, Antioxidant, 2,2-diphenyl-1-picrylhydrazyl, Ascorbic acid.

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AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAN FRAKSI AKAR TABAR KEDAYAN (Aristolochia foveolata Merr.) DENGAN METODE DPPH (2,2 DIPHENYL-1-PICRILHYDRAZIL)

JFL : Jurnal Farmasi Lampung ◽

10.37090/jfl.v8i1.81 ◽

2019 ◽

pp. 8-14

Author(s):

Risa Supringrum ◽

Siti Jubaidah

Keyword(s):

Antioxidant Activity ◽

Free Radicals ◽

Ethyl Acetate ◽

Ethanol Extract ◽

Ethyl Acetate Fraction ◽

The Body ◽

Hexane Fraction ◽

High Dose ◽

Water Fraction ◽

Dpph Method

Antioxidants are substances that can neutralize free radicals, thus protecting the body from various diseases by binding to free radicals and highly reactive molecules that can damage cells. The use of high-dose synthetic antioxidants is reported to be toxic and carcinogenic. Tabar Kedayan root (Aristolochia foveolata Merr) is one of the native plants from North Kalimantan, which is empirically used as an anti-poison, containing secondary metabolites including alkaloids, tannins, flavonoids. Flavonoids are powerful antioxidants. Some studies report that the function of flavonoids can be to prevent and treat cancer. This study aims to determine the antioxidant activity of ethanol extract and n-hexane fraction, the ethyl acetate fraction of Tabar Kedayan root with 2.2 Diphenyl- 1-Picrilhydrazil (DPPH) method as a free radical compound. The results of the study obtained IC50 values for each sample, at ethyl acetate fraction 267.48 ppm, ethanol extract at 603.80 ppm, ethanol-water fraction 705.43 ppm, n- hexane fraction 1500 ppm. The antioxidant activity tests indicate that ethyl acetate fraction is categorized as weak antioxidant, while the ethanol-water fraction and n-hexane fraction exhibit no antioxidant activity. Keywords : Tabar Kedayan, Antioxidants, 2.2 Diphenyl-1-Picrilhydrazil

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Antioxidant activity of Ethanol Extract, n-Hexane fraction, Ethyl Acetate fraction and Water fraction of Garut Orange Leaves (Citrus reticulata Blanco)

Journal of Physics Conference Series ◽

10.1088/1742-6596/1402/5/055083 ◽

2019 ◽

Vol 1402 ◽

pp. 055083

Author(s):

F M Fadhlillah ◽

W Oktaviani ◽

R Mariani

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Ethanol Extract ◽

Ethyl Acetate Fraction ◽

Hexane Fraction ◽

Citrus Reticulata ◽

Water Fraction ◽

Citrus Reticulata Blanco

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PENENTUAN TOTAL FENOLIK DAN AKTIVITAS ANTIOKSIDAN FRAKSI ETIL ASETAT DARI TEPUNG PELEPAH AREN (Arenga pinnata)

JURNAL ILMIAH SAINS ◽

10.35799/jis.16.2.2016.14067 ◽

2016 ◽

Vol 16 (2) ◽

pp. 68

Author(s):

Yudhie E Prasetyo ◽

Meiske S Sangi ◽

Audy D Wuntu

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Total Phenolic Content ◽

Phenolic Content ◽

Radical Scavenging ◽

Ethyl Acetate Fraction ◽

Total Phenolic ◽

Ic50 Value ◽

Dpph Radical Scavenging

PENENTUAN TOTAL FENOLIK DAN AKTIVITAS ANTIOKSIDAN FRAKSI ETIL ASETAT DARI TEPUNG PELEPAH AREN (Arenga pinnata) ABSTRAK Tujuan penelitian ini untuk menentukan total fenolik dan aktivitas antioksidan fraksi etil asetat dari tepung pelepah aren (Arenga pinnata). Tepung pelepah aren diekstraksi maserasi menggunakan etanol hasil redestilasi cap tikus kemudian di partisi menggunakan pelarut petroleum eter, etil asetat dan aquades. Penentuan kandungan total fenolik menggunakan metode Folin-Ciocalteu dan aktivitas antioksidan menggunakan metode serapan radikal 1,1-difenil-2-pikrilhidrazil (DPPH). Didapati kandungan total fenolik fraksi etil asetat sebesar 172.857 ± 4.762 mg/kg dan aktivitas penangkal radikal bebas fraksi etil asetat sebesar 90.573 µg/mL. Hasil ini menunjukan bahwa fraksi etil asetat berpotensi sebagai antioksidan alami. Kata kunci: Tepung Pelepah aren, fenolik, aktivitas antioksidan, DPPH DETERMINATION TOTAL PHENOLIC CONTENT AND ANTIOXIDANT ACTIVITY ETHYL ACETATE FRACTION OF AREN’S MIDRIB POWDER (Arenga pinnata) ABSTRACT This research aims to determine the total phenolic content and antioxidant activity from ethyl acetate fraction of Aren’s midrib powder (Arenga pinnata). Aren’s midrib powder extracted with maceration method with cap tikus redistillated ethanol and then partitioned with solvents such as petroleum ether, ethyl acetate and distillate water. The determination of phenolic content was using Folin-Ciocalteu method and the antioxidant activity was done using diphenylpycrylhydrazyl (DPPH) radical scavenging. Total phenolic value of ethyl acetate fraction is 172.857 ± 4.762 and IC50 value of ethyl acetate fraction is 90.573 µg/mL. This result shows ethyl acetate fraction as natural antioxidant potential. Keywords: Aren’s midrib powder, phenolic, antioxidant activity, DPPH

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Phytochemical Screening and Antioxidant Activity of Date(Phoenix Dactylifera) Seed Extracts

International Journal of Recent Technology and Engineering - 2 ◽

10.35940/ijrte.b1018.0782s719 ◽

2019 ◽

Vol 8 (2S7) ◽

pp. 87-89

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Methanol Extract ◽

Ethyl Acetate Fraction ◽

Hexane Fraction ◽

Phytochemical Screening ◽

Water Fraction ◽

Ic50 Value ◽

Dpph Method

Dates consisting of 90% flesh have been known to have various benefits, but the remaining 10% of them in the form of seeds has not been utilized optimally. The date palm contains a fairly high polyphenol compound, which can act as an antioxidant. This study aims to determine the antioxidant activity of methanol extract and fractionation of the extract of dates using the in vitro method of DPPH (1,1-diphenyl-2-picrilhidrazil). The date seeds are made into simplicia, macerated with methanol, and then the extracted methanol is gradually fractionated by n-hexane, ethyl acetate, n-butanol, and water. Each fraction and extract is phytochemically screened using the standard methods and its antioxidant activity is determined by DPPH method. Methanol extract, ethyl acetate, n-butanol, and water fractions contain tannins, flavonoids, alkaloids, and saponin, while the n-hexane fraction not contain phytochemical compound based on the phytochemical screening. The ethyl acetate fraction has the strongest antioxidant activity with the lowest IC50 value, that is equal to 3.72 ± 0.44 μg/mL, followed by methanol extract of 4.71 ± 0.64 μg/mL, n-butanol fraction of 6.62 ± 0.08 μg/mL, water fraction of 53.00 ± 18.96 μg/mL, and n-hexane fraction of 250.19 ± 9.69 μg/ mL, while the IC50 value of vitamin C as the positive control is 4.29 ± 0.74 μg/mL. Therefore, date seeds can be a source of natural antioxidants

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ANTIOXIDANT ACTIVITY OF TAUCO ETHANOL EXTRACT AND ITS FRACTIONS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10s2.19488 ◽

2017 ◽

Vol 10 (14) ◽

pp. 57

Author(s):

Resmi Mustarichie ◽

Sandra Megantara ◽

Wiwiek Indriyati ◽

Ade Zuchrotun

Keyword(s):

Antioxidant Activity ◽

Vitamin C ◽

Ethyl Acetate ◽

Ethanol Extract ◽

Ethyl Acetate Fraction ◽

Extraction Methods ◽

Phytochemical Screening ◽

Water Fraction ◽

Modified Method ◽

Ic50 Value

Objective: To investigate antioxidant activity and phytochemical screening of ethanol extract, fractions of water, ethyl acetate and n-hexane from tauco.Methods: Two types of tauco was extracted using soxhletation methods, followed by fractionation using liquid-liquid extraction methods and phytochemical screening. Antioxidant activity test was carried out using DPPH with Ascorbic acid (vitamin C) as a reference. Modified method of Farnsworth was applied for phytochemical screening.Results: It was found that extracts of ethanol and ethyl acetate fraction containing flavonoids, monoterpenoid, and sesquiterpenoids whereas the water fraction and a fraction of n-hexane only contain monoterpenes and sesquiterpenoids. The IC50 value for the ethanol extract, water, ethyl acetate, and n-hexane fractions of two taucos in a row were 1192.71 ppm, 1746.01 ppm, 722.38 ppm, 1845.45 ppm and 1190,15 ppm, 1740.30, 710.46, for tauco A and B respectively.Conclusion: It was unexpected that tauco ethanol extract and fractions showed much weaker antioxidant activity than vitamin C, which had the IC50 value of 4.41 ppm.

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Ethanol Extract, Ethyl Acetate Extract, Ethyl Acetate Fraction, and n-Heksan Fraction Mangosteen Peels (Garcinia mangostana L.) As Source of Bioactive Substance Free-Radical Scavengers

JPSCR Journal of Pharmaceutical Science and Clinical Research ◽

10.20961/jpscr.v1i2.1936 ◽

2016 ◽

Vol 1 (2) ◽

pp. 71

Author(s):

Liza Pratiwi ◽

Achmad Fudholi ◽

Ronny Martien ◽

Suwidjiyo Pramono

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Ethanol Extract ◽

Ethyl Acetate Fraction ◽

Hexane Fraction ◽

Garcinia Mangostana ◽

Ic50 Value ◽

Dpph Method ◽

Peel Extract

Mangosteen peels (Garcinia mangostana L.) is well known as an excellent source of antioxidative compounds. The name of mangosteen is xanthone. Antioxidant of mangosteen peels can be extracted by ethanol, etyl acetate and can be fractinated by etyl acetate and n-hexane. The aim of this research was to compare the antioxidant activity of the peel extract by ethanol and etyl acetate and fractinated by etyl acetate and n-hexane. Extract and fraction exhibited higher scavenging activity of DPPH. The purpose of this study was to compare antioxidant activity of ethanol extract, ethyl acetate extract, fraction of ethyl acetate and n-hexane fraction. The antioxidant activity test using DPPH method with UV-Vis spectrophotometer. Ethanol extract shown IC50 value 5,03 µg/mL, ethyl acetate extract shown IC50 value 41,56 µg/mL. Ethyl acetate fraction shown IC50 value 2,78 µg/mL, and n-hexane fraction with IC50 22,33 µg/mL. It means peel extract and fraction by mangosteen peels has very strong antioxidant activity and ethyl acetate fraction that its antioxidant activity higher that the other solvent.

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Antioxidant Activity, Inhibition α-Glucosidase of Ethanol Extract of Strychnos nitida G. Don and Identification of Active Compounds

Current Biochemistry ◽

10.29244/cb.5.3.11-20 ◽

2019 ◽

Vol 5 (3) ◽

pp. 11-20

Author(s):

Stefani Dhale Rale ◽

Hasim Hasim ◽

Syamsul Falah

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Ethanol Extract ◽

Ethyl Acetate Fraction ◽

Inhibition Activity ◽

Active Compounds ◽

Activity Inhibition ◽

Activity Test ◽

Ic50 Value ◽

Treatment Of Diabetes

This study aims to find the treatment of diabetes using natural materials by exploring plants in the province of East Nusa Tenggara. his research was conducted out by extracting the Strychnos nitida G.Don stem using a method of maceration by ethanol 70%. Ethanol extract was then fractionated using n-hexane and ethyl acetate. Simplicia from maceration and fractionation results were then tested for antioxidant activity, α-glucosidase inhibition activity and identification of active compounds. The results showed that ethyl acetate fraction had the lowest IC50 value of 86.83 μg / ml. Results of the α-glucosidase activity test showed that ethyl acetate fraction and n-heksan fraction at 900 ppm had the highest percentage of inhibition of 34.23% and 33.89%. Identification using LCMS/MS method showed that ethyl acetate fraction consist of Benzenemethamine, N, N-dioctyl- as an antioxidantcompound and compound 24-methyl-5-cholestone-hexol as an antidiabetic compound. From the results of this study, we concluded that the extract of kayu ular Strychnos nitida G.Don stem has inhibition activity toward α-glucosidase enzyme.

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Comparative Antioxidant Activity on The Ficus benjamina and Annona reticulata Leaves

International Journal of Public Health Science (IJPHS) ◽

10.11591/ijphs.v4i1.4707 ◽

2015 ◽

Vol 4 (1) ◽

pp. 21

Author(s):

Nyi Mekar Saptarini ◽

Irma Erika Herawati

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Ethanolic Extract ◽

Natural Antioxidants ◽

Ethyl Acetate Fraction ◽

Hexane Fraction ◽

Ficus Benjamina ◽

Water Fraction ◽

Free Radical Formation ◽

Annona Reticulata

Antioxidants can prevent free radical formation. Natural antioxidants found in many plants, such as Ficus benjamina and Annona reticulata. The study aimed to compare the antioxidant activity of extracts and fractions of Ficus benjamina and Annona reticulata leaves against 1,1-diphenyl-2-picrilhydrazyl. The steps of this study consist of extraction, fractionation with n-hexane, ethyl acetate and water, phytochemical screening, antioxidant activity determination, and comparing the IC50 values. Percentage scavenging activity of the extracts and fractions against DPPH was calculated to determine the antioxidant activity. The IC50 value of Ficus benjamina was 127.86 ppm for ethanolic extract, 94.01 ppm for water fraction, 115.48 ppm for ethyl acetate fraction, and 335.50 ppm for n-hexane fraction. The IC50 value of Annona reticulata was 274.31 ppm for ethanolic extract, 211.42 ppm for water fraction, 367.91 ppm for ethyl acetate fraction, and 741.08 ppm for n-hexane fraction. The results showed that the Ficus benjamina water fraction was the best antioxidant compared to other extract and fraction.

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