scholarly journals Zincum metallicum 5cH increases survival and improves clinical mice infected with Trypanosoma cruzi

2021 ◽  
Vol 13 (47) ◽  
pp. 111-113
Author(s):  
Larissa Ciupa ◽  
Franciele Karina Da Veiga ◽  
Angela Rigo Portocarrero ◽  
Patricia Flora Sandri ◽  
Fabiana Nabarro Ferraz ◽  
...  

The Multicenter International Project suggests Zincum Mettalicum high diluted as object of study in different experimental models. Aim: evaluate the effect of substance high diluted Zincum metallicum in murine experimental infection by Trypanosoma cruzi. Metodology: was performed a blind, controlled, randomized, using 60 swiss male mice, 56 days old, divided into groups: CNI - uninfected and untreated animals; CI - infected and untreated animals; infected and treated animals: ZN5cHTA - Zinc 5ch and LAC5cHTA - Lactose 5ch , 48 hours before and after infection, subsequently were treated 56/56 hours until 9th day of infection; ZN5cHTTD - Zinc 5Ch and LAC5cHTTD - Lactose 5cH, everyday from the 4th of infection. Animals were inoculated with 1.400 blood trypomastigotes, strain Y-T. cruzi, intraperitoneally. Medicines were handled, prepared in grain alcohol 70%, and dynamized up to 100 times until 4cH. To obtain the 5cH it was used bi-distilled sterilized water filtered in membrane - 0.22 µm [1], on separate days (first Lactose and then Zinc) and stored in different rooms. Microbiological test in vivo and toxicity were made in accordance with current legislation [2].Test solutions were diluted in water at a concentration of 10% (1mL/100mL) after dilution in water. Clinical (temperature, weight, water/foodintake and excreta)[3] and parasitological parameters (pre-patent and patent period, peak parasitemia, and parasitemia overall survival time)[4] were assessed daily. Data were compared BioEstat 5.0, significance level of 5%. Project was approved by the Ethics Committee on Animal Use in Experimentation of the Universidade Estadual de Maringa by opinion number 025/2014. Results: ZN5cHTA group had a higher survival rate than their control LAC5cHTA (p=0.004). ZN5cHTA shows 55.7% probability of surviving to the 15th day after infection, while LAC5cHTA 29.4%. ZN5cHTA also provides significantly better performance (p= 0.0206) compared to CI, contrary to what occurs with LAC5cHTA x CI (p=0.7410). There is no significant difference in survival between the different treatments schemes TA and TTD, either with ZN5cH (p=0.0754) or LAC5cHTA (p=0.9480), although the best ZN5cHTA present trend toward benefit. Considering parasitological parameters ZN5cHTA group had higher pre-patent period (PPP) meaning benefit to infected animals [5]. Although ZN5cHTA shows greater number of parasites from 6th to 11th day of infection and right shift of parasitemia peak in relation to LAC5cHTA (p=0.020), this group displayed a better performance compared to the other groups as observed in other models [6]. Conclusion: ZN5cHTA group had higher survival, greater pre-patent period and better clinical outcome compared to control LAC5cHTA and to other groups. This result may be related to higher total parasitemia and alterations in the parasite cycle time observed in this group. These findings suggest aggravation with posterior benefit as reported in some cases of homeopathic treatment.

2014 ◽  
Vol 1 (3) ◽  
pp. 230-235
Author(s):  
Utami Dewi ◽  
Fidyah Aminin ◽  
Harvensica Gunnara

Sleep is an essential need that must be met, especially in the development phase since brain and body development will occur during sleep. Thus, sleep disturbance is a problem that will cause adverse effects on growth and development, especially for babies. Sleep quality for babies can be improved by providing baby massage on a regular basis. The purpose of this study was to prove whether there were differences in sleep quality before and after the massage in babies aged 3-4 months in Gelatik and Nuri IHC (Integrated Health Center) of Tanjungunggat Village of Tanjungpinang Municipality. Design: This was a Quasi Experiment study with a pretest-posttest method. The sample consisted of 17 respondents selected by purposive sampling technique. The statistical test used in this study was a dependent t-test with a significance level of 0.05.Result: The results showed that there was no significant difference between baby sleep duration before and after the massage (p = 0.414). However, there was significant difference between the frequency of waking up before and after the massage (p = 0.001) and there was a significant difference between the duration of waking up before and after the massage (p = 0.046. Discuss: Based on the results of this study, it is suggested that mothers should improve their knowledge on the importance of baby massage provided by health professionals and the health professionals should provide and demonstrate clear, complete and sustained information to the mothers and other related parties surrounding the IHC.


2020 ◽  
Vol 2020 ◽  
pp. 1-6 ◽  
Author(s):  
Abdulmohsen Alfadley ◽  
Abdalrhman Alrajhi ◽  
Hamad Alissa ◽  
Faisal Alzeghaibi ◽  
Lubna Hamadah ◽  
...  

The aim of this study was to assess the shaping ability of the XP Shaper (XPS) file in severely curved canal models under simulated body temperature and compare it with that of the WaveOne Gold (WOG) file. Ninety-six simulated root canals were equally distributed into XPS and WOG systems to be shaped by eight files each. Files were assessed under a stereomicroscope prior to canal shaping to detect deformation if any. The canals were shaped at 35 ± 1°C using the X-Smart Plus motor. Images of the canals were obtained before and after instrumentation using a stereomicroscope to measure the amount of removed resin from both the inner and outer curvature sides at apex (0 mm) and 3 mm and 6 mm from the apex. The shaping time was calculated. The data were statistically analyzed by the independent t-test at 5% significance level. The XPS and WOG systems shaped the canals in 37.0 ± 9.5 and 62.6 ± 11.3 seconds (P<0.05), respectively. At the apex level, the amount of resin removal in both sides did not show a significant difference between the tested groups (P>0.05). At 3 mm and 6 mm levels, the WOG removed more resin than XPS at both sides (P<0.05). In XPS, deformation was observed in four files: one file after the first use, one file after the fourth use, and two files after the sixth use. In WOG, two files were deformed: one file after the fifth use and one file after the sixth use. One XPS file was fractured after the sixth use. In short, XPS and WOG files can be used in shaping severely curved canals as they showed the ability to maintain the original shape with minimal transportation. Both file systems showed signs of deformation after use with a lower number of deformed files observed in WOG throughout the experiment.


1977 ◽  
Author(s):  
H. Yamazaki ◽  
T. Motomiya ◽  
M. Sonoda ◽  
N. Miyagawa

Substantial clinical evidence indicates that large doses of estrogen frequenly result in thromboembolic disorders. Effects of estrogen on platelet aggregability were examined in women with uterine myoma before and after oophorectomy. Bilateral oophorectomy on 15 cases (48.7+0.12 yrs, mean+SE) and unilateral or no oophorectomy on 18 cases (control group : 42.2+0.18 yrs) were performed with myomectomy of the uterus. On one day before and one day, one week and one month after the operation performed, their platelet count by Coulter counter, platelet volume by Coulter channelyzer and platelet aggregability by Sienco aggregometer were measured. 24 hrs total estrogen in urine was also determined. In the control group, platelet counts were 85.1+ 4.9 % of the preoperated value one day after, 127.9+9.0 % one week after and 98.1+7.6 % one month after the operation. In the bilateral oophorectomy group, these were 82.4+5.2 % one day after, 124.0+4.7 % one week after and 96.1+4.8 % one month after. Both the groups showed the same change. Platelet aggregability by 3 μM ADP were 76.9+14.3 % one day after, 203.0+57.1 % one week after and 193.4+59.0 % one month after in the control, while 55.0+13.6 % one day after, 102.5+12.9 % one week after and 60.6+14.7 % one month after the operation in the total oophorectomy group. There was a statistically significant difference in the values obtained one month after the operation between the groups (p<0.05). Characteristic changes in platelet volumes were also observed. A significant correlation was observed between the platelet aggre-gabilities and the daily urinary estrogen excretion levels. The above results suggest that estrogen may enhance platelet aggregability in vivo.


Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 1234-1234 ◽  
Author(s):  
Laura M. De Castro ◽  
Jude C. Jonassaint ◽  
Jennifer G. Johnson ◽  
Milena Batchvarova ◽  
Marilyn J. Telen

Abstract Sickle red blood cells (SS RBC) are abnormally adhesive to both endothelial cells (ECs) and components of the extracellular matrix (ECM). Epinephrine (epi) has been shown to elevate cAMP in SS RBC and increase adhesion of SS RBC to ECs in a protein kinase A-dependent manner. In vitro and in vivo studies performed in our lab have led to the hypothesis that adrenergic stimuli such as epi may initiate or exacerbate vaso-occlusion and thus contribute to the association of vaso-occlusive events with physiologic stress. We are conducting a prospective, dose-escalation pilot clinical study to investigate whether in vivo administration of one dose of propranolol either down-regulates baseline SS RBC adhesion in vitro or prevents its upregulation by epi. In addition, this study will provide additional safety data regarding the use of propranolol in normotensive patients with sickle cell disease (SCD). Figure Figure To date, we have completed the first two dose cohorts. 11 subjects (9 SS and 1 Sβ° thalassemia; 7 females, 3 males) have participated. No severe adverse events were noted. Cohorts 1 and 2 had mean pre-propranolol blood pressure (BP) of 116 (5.9 SD)/ 60.4 (3.98 SD) and 106.8 (4.68 SD)/ 58 (3.9 SD), respectively; this difference was not statistically significant. Minimal and asymptomatic changes in BP were noted in both cohorts after drug administration, with biphasic systolic and diastolic BP nadirs at 45 and 240 minutes. No clinically significant changes in heart rate were observed. Adhesion studies were performed using a graduated height flow chamber on the day of RBC collection. RBC adhesion to ECs was studied before and after epi stimulation and was measured at sheer stresses ranging from 1 to 3 dyne/cm2. Baseline adhesion measurements were validated by comparing percent (%) adhesion assayed at 2 different times within 7 days—at screening and before propranolol dose on the study drug day. We observed no significant difference in adhesion at the 2 different time points without propranolol. Comparison of % adhesion of epi-stimulated RBC to ECs before and 1 hour after propranolol showed that propranolol given in vivo significantly inhibited both non-stimulated and epi-stimulated SS RBC adhesion (p=0.04 and p=0.001, respectively). Lastly, comparison of SS RBC adhesion at both drug doses confirmed the drug-related inhibition of adhesion (p&lt;0.004). We conclude that propranolol administered in vivo decreases SS RBC baseline adhesion to ECs and substantially abrogates epi-stimulated adhesion to ECs, as measured in vitro. Although we have thus far studied only a small number of patients and low propranolol doses, we expect to confirm these results with the 3rd cohort, in which a higher dose of propranolol will be used. If our findings continue to show that propranolol can decrease both SS RBC baseline and epi-stimulated adhesion to ECs, study of propranolol on a larger scale would be warranted in order to ascertain its safety and efficacy as an anti-adhesive therapy in SCD.


2021 ◽  
Vol 10 (36) ◽  
pp. 163-166
Author(s):  
Denise Lessa Aleixo ◽  
Erika Cristina Ferreira ◽  
Caroline Felicio Braga ◽  
Camila Fernanda Brustolin ◽  
Mônica Lúcia Gomes ◽  
...  

Introduction: The study of the effect of different ways of treatment using highly diluted substances is rare in the literature. Some authors consider the dose irrelevant, justifying that the action of the medication highly diluted is qualitative [1-3]. Others emphasize the importance of quantity and frequency of administration of the highly diluted substance for a successful treatment [4,5]. The model of murine infection by T. cruzi is widely studied and it is an excellent tool to study the effect of highly diluted substances. Aim: To evaluate, in vivo, the effect of different amounts and frequency of administration of the biotherapic 17 dH T. cruzi in the evolution of the parasitemia curve and survival of mice infected with Trypanosoma cruzi. Materials and methods: A blind randomised controlled trial was performed, using 30 swiss male mice, aged 28 days, divided into groups according to treatment: CONTROL - mice treated with 7% water-alcohol solution diluted in water given ad libitum in an amber bottle; GAVAGE – mice treated with medication highly diluted 17 DH T. cruzi from 4 th to 9 th day of infection by gavage; WATER - mice treated with highly diluted medication 17 DH T. cruzi in water ad libitum offered in an amber bottle until the end of the study period. The groups were infected with the Y strain of T. cruzi, intraperitoneal, 1400 blood trypomastigotes. The medicines was handled according to the Brazilian Homeopathic Pharmacopoeia [6] with microbiological test according to RDC n°. 67 and in vivo biological risk. Parasitemic curve was determined by daily counting of the parasites [7], the total parasitemia, peak parasites and survival. Data were compared using the BioEstat 5.0, ANOVA, with significance of 5%. The experiment was approved under the protocol n° 030/2008 - Ethics in Animal Experimentation of the Universidade Estadual de Maringá. Results: Animals treated with the medication highly diluted in water had lower level of total parasitemia and a lower peak of parasites compared to animals treated by gavage, or control group of infection (p = 0.0103 p = 0.0008). In the group treated by gavage both the total parasitemia and the peak of parasites were higher than the control group. Survival was greater in animals treated with biotherapic diluted with water (p = 0.0003) and by gavage (p = 0.0016) when compared with the control group. Among the different ways of treatment the use of medication diluted in water increased the survival of animals (p = 0.0013). The treatment by gavage once a day until the 9th day of infection increase the parasitemia and survival. The medication diluted in water showed better results with significant reduction of parasitemia and an increase of survival. This result may be related to the frequency with which the medication diluted in water was ingested by each animal, and the lower stress that this form of administration provides the animals. Figure 1: Parasitemic curve of animals infected with Y strain of T. cruzi and treated with medication highly diluted 17DH T. cruzi. CONTROL: mice treated with alcohol 7%; GAVAGE: treated with medication highly diluted 17DH T. cruzi by gavage; WATER: treated with medication highly diluted 17dH T. cruzi in water. Conclusion: There is a difference in the effect of the medication highly diluted depending on the way of treatment used. For mice, the use of drug diluted in water offered frequently, results in better benefits. The clinical use of these results in humans, should consider the allometric system medication dosage which takes into account the metabolic rate of each organism.


2020 ◽  
Vol 12 (1) ◽  
pp. 50
Author(s):  
Komang Lia Karina ◽  
I Nyoman Sujana ◽  
M. Rudi Irwansyah

This study aimed to analyze the reaction of investors on Indonesia Stock Exchange to the inauguration of the 8th President by observing whether there were any significant differences in abnormal returns and stock trading volume activities before and after the event. The observation period used in this study was 10 days, with details of each 5 days before and after the President's inauguration event that occurred on 20 October 2019. This research was quantitative research and used daily transaction data on the market capital as a secondary data source. The samples used were companies that were included in the LQ45 stock index for the period August 2019 - January 2020. A non-parametric test in the form of Wilcoxon test was used to test the hypothesis. The results of this study showed that there were no significant difference in abnormal return and stock trading volume activity in the period before and after the event. This was evidenced by the probability value above the significance level of 5%. Thus, the results of this study were stated that there was no reaction from the investor related to the event of the inauguration of the 8th President in Indonesia.


BIODIK ◽  
2019 ◽  
Vol 5 (2) ◽  
pp. 150-163
Author(s):  
Dewa Ayu Sri Ratnani

This study aims to analyze whether the application of Group Investigation (GI) learning model Based on Mind Mapping media can improve   creativities of the student of SMP Wisata Sanur.  The research type is Pre-Exprimental Design with One-group Pretest-Postest Designs research design conducted at SMP Wisata Sanur from April to May. Data collection using creativity assessment rubrics with 7 aspects. The result showed that there was an increase of creativity score from 75,3 to 169,2 with significance level of each aspect of creativity, ie Design aspect (p = 0,017), Color (p = 0,017), Idea and Context (p = 0,017), Kata Key (p = 0.018), Combining ideas (p = 0.018), Hierarchy Level (p = 0.017), Presentation Concept (p = 0.017). Based on the data obtained after being tested with Wilcoxon Match Pairs there was a significant difference (p = 0,018> 0,05) on student creativity in making Mind Mapping before and after apply the learning model. The conclusion of this research is the application of cooperative learning model of Group Investigation based on Mind Mapping has an effect on to the creativity of junior high school students of Sanur Tourism. Keywords: Group Investigation, Creativity, Mind Mapping   Abstrak. Penelitian ini bertujuan untuk menganalisis apakah penerapan model pembelajaran Group Investigation (GI) Berbasis media Mind Mapping dapat meningkatkan kreativitas siswa SMP Kerta Wisata Sanur. Jenis penelitian adalah Pre-Exprimental Design dengan rancangan penelitian One-group Pretest-Postest Designs yang dilaksanakan di SMP Wisata Sanur . Data didapatkan menggunakan rubrik penilaian kreativitas dengan 7 aspek. Hasil penelitian menunjukkan terdapat peningkatan jumlah skor kreativitas dari 75,3 menjadi 169,2 dengan taraf signifikansi masing-masing aspek kreativitas yaitu aspek Rancangan (p=0,017), Warna (p=0,017), Ide dan Konteks (p=0,017), Kata Kunci (p=0,018), Menggabungkan ide-ide (p=0,018), Tingkat Hirarki (p=0,017), Konsep Presentasi (p=0,017).  Hasil uji dengan Wilcoxon Match Pairs terdapat perbedaan yang signifikan (p= 0,018 > 0,05) pada kreativitas siswa dalam membuat Mind Mapping sebelum dan sesudah menerapkan model pembelajaran kooperatif  tipe Group Investigation. Kesimpulan dari penelitian ini adalah penerapan model pembelajaran kooperatif  tipe Group Investigation berbasis Mind Mapping  mampu meningkatkan kreativitas siswa SMP Wisata Sanur.    Kata kunci : Group Investigation, Kreativitas, Mind Mapping


2018 ◽  
Vol 16 (2) ◽  
pp. 51-55
Author(s):  
Catiana Leila Possamai Romanzini ◽  
Beatriz Lara Leile Pavanello ◽  
Kessi Cassiane Iarosz ◽  
Vinícius Zandonadi Pires

Estratégias de intervenção que utilizam exergames que visam o estímulo à prática de atividade física, bem como à redução do tempo em comportamento sedentário, parecem ser caminhos promissores para lidar com o aumento das taxas de obesidade. Este foi o intuito do Projeto de Extensão “Exergaming para a saúde: intervenção para o controle do peso corporal em adolescentes utilizando videogames ativos”, contemplado com fomento do MEC/PROEXT no ano de 2013. O objetivo do estudo foi verificar o efeito de uma intervenção de oito semanas com videogames ativos sobre variáveis relacionadas ao peso corporal de adolescentes. Foi realizado um estudo piloto de intervenção de oito semanas com duração de 60 minutos por sessão, com uso dos exergames. Participaram do estudo oito escolares, com média de idade de 11,5±0,5 anos. Medidas antropométricas antes e após a intervenção foram realizadas. Utilizou-se a estatística descritiva e o teste t pareado, no SPSS 20.0 com nível de significância de p0,05. Observou-se que houve diferença estatisticamente significativa somente na circunferência de cintura (cm) com redução de 2,8 cm (p = 0,028). As demais variáveis analisadas não foram diferentes. O estudo piloto de uma intervenção de oito semanas com videogames ativos demonstrou possibilidade de redução de medidas antropométricas, tais como a circunferência de cintura dos adolescentes. Sugere-se que novos estudos com exergames tenham enfoque neste desfecho em um período mais longo de intervenção e/ou com maior número de sessões durante a semana.ABSTRACT. Intervention for the control of body weight in adolescents using active video games: a pilot study. Intervention strategies that use exergames that aim to stimulate the practice of physical activity, as well as the reduction of the time in sedentary behavior, through promising ways to deal with the increase of obesity rates. This is the way of Extension Project “Exergaming for health: intervention for the control of body weight in adolescents using active video games”, with fomentation of MEC/PROEXT in 2013. The aim of this study was to verify the effect of an eight-week intervention with active video games on variables related to adolescent body weight. An eight-week intervention study with a duration of 60 minutes per session, with use of exergames was performed. Eight schoolchildren participated, with mean age of 11.5±0.5 years. Anthropometric measures before and after the intervention were performed. Descriptive statistics and the non-parametric equivalent of paired t-test were used in SPSS 20.0 with significance level of p0.05. It was observed that there was a significant difference only in waist circumference (cm) with a reduction of 2.8 cm (p = 0.028). The other variables analyzed were not different. The pilot study of an eight-week intervention with active videogames demonstrated the possibility of reducing anthropometric measures, such as the waist circumference of adolescents. We suggested that new studies with exergames focus on this outcome over a longer period of intervention and/or with a larger number of sessions during the week. 


2019 ◽  
Vol 5 (2) ◽  
pp. 112-123
Author(s):  
Fariba Javazi ◽  
◽  
Parisa Sedaghati ◽  
Hasan Daneshmandi ◽  
◽  
...  

Objective Upper Crossed Syndrome (UCS) is a type of musculoskeletal system involvement that results in shortening of anterior muscles and weakening of posterior muscles. The aim of this study was to examine the effect of selected corrective exercises with physioball on the postural status of female computer users with UCS. Methods This quasi-experimental study was conducted on 24 female students with UCS who were selected using a purposive sampling method. The subjects were randomly divided into two groups of exercise (n=12) and control (n=12). The exercise group performed exercises for 6 weeks. Photogrammetry method was used to measure the angle of the forward head and round shoulder. A flexible ruler was used to measure the angle of kyphosis, and tape measure was used to measure the chest expansion. These measurements were performed before and after intervention. The collected data were analyzed using paired t-test and ANOVA at the significance level of P<0.05. Results There was a significant difference in mean values of kyphosis (P=0.001), round shoulder (P=0.001), forward head (P=0.002) and chest expansion (P=0.003) before and after exercise. Conclusion Improvement in forward head, round shoulder and thoracic kyphosis angles and chest expansion showed the effectiveness of applied exercise program. Therefore, it is recommended to use this exercise program in computer users with UCS.


2018 ◽  
Vol 9 (1) ◽  
pp. 68-76
Author(s):  
Iman Sulaiman ◽  
Ahmad Rizaldy Fajrin

The objective of the research and development of the training model is to attack on the game of basketball. In addition, research and development is conducted to obtain in-depth information about the development and implementation of the exercise model and to know the effectiveness, efficiency of the model made.This research and development uses Research & Development (R & D) development methods from Borg and Gall. The subjects in this research and development are 2 different two place clubs of Victoria basketball club, Level Up basketball club.The model effectiveness test uses a test instrument to collect the basketball player's ability data. Analysis of research data using t test with significance level of 0.05. Analysis of data obtained average attack value before given model of attacking exercise is 61.0455 or 61 and after being given treatment with attacking model 75.5909 or 75.6. In the test of significance difference with SPSS 16 can result t-count = -29,567 db = 44 and p-value = 0.00 <0.05 which means there is a significant difference in the ability to attack before and after being given an attacking training model on basketball game that with a significance level of 0.05 then H0 is rejected. Based on the description it can be concluded that the training model attack on basket game developed that can be applied effectively and efficiently so that it can increase attack on basketball players.


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