scholarly journals Metastatic melanoma cells are selective affected in vitro by Atropa belladonna 200 c.

2021 ◽  
Vol 17 (2) ◽  
pp. 19-20
Author(s):  
Maria Luiza Ferreira dos Santos ◽  
Jenifer Pendiuk Gonçalves ◽  
Viviana Stephanie Costa Gagosian ◽  
Gustavo Rodrigues Rossi ◽  
Edvaldo Da Silva Trindade ◽  
...  
Keyword(s):  
Tumor Progression ◽  
Metastatic Melanoma ◽  
Survival Rates ◽  
Melanoma Cells ◽  
Chemotherapeutic Agents ◽  
Control Group ◽  
P Value ◽  
Atropa Belladonna ◽  
Antitumor Effects

Abstract Background: Cancers are among the top 10 causes of death worldwide. Melanoma is a skin cancer originated from mutations on melanocytes. Transformed melanocytes can migrate and invade tissues in a process called metastasis. When early diagnosed, melanoma is curable by surgical excision. However, when metastatic, cells are refractory to existing therapies and patient survival rates are low. New therapeutic interventions with effective results on melanoma cells that could increase long term patients’ survival rates are needed. Over the past decade our group has been using cell-based models to determine highly diluted solutions effects. Several compounds have been tested on normal and tumor cells with promising results that could indicate the efficacy of homeopathy on selected cases. Aims: To investigate Atropa belladonna 200 c (Bell200c) in vitro antitumor effects on B16-F10 metastatic murine melanoma cells and its toxicity on Balb/3T3 normal murine fibroblasts. Methodology: Atropa belladonna 200c was produced and kindly donated by “Homeoterápica” compounding pharmacy (Curitiba, Brazil). Cells were treated for up to 72 hours. Assays to determine cytotoxicity, as well as functional and molecular cell patterns were carried out following standard protocols. Water treated cells were used as control group. All data were submitted to Shapiro-Wilk normality test followed by either Mann-Whitney or t-test. P value < 0.05 was considered significant. Results and discussion: Cancer cells accelerated proliferation rates are often target for chemotherapeutic agents. Bell200c modulated cell cycle, leading to a decreased melanoma cell proliferation (44% less cells then control), and an increase in apoptotic cells number. If unspecific, those effects can lead to undesirable toxicity. Amazingly, no effects on fibroblast proliferation and death were observed. Melanoma cells malignancy was also affected, as the following tumor progression related features were modulated. Melanin production was higher in treated cells. N-cadherin and CD44 expression were statistically decreased. Clonogenic capacity was decreased by 35%. Here we have demonstrated Bell200c selective effects on melanoma cells with remarkable capacity of impairing metastatic melanoma characteristics. Conclusion: Atropa belladonna has a prospective use in metastatic melanoma patients as a potential tumor progression regulator. Keywords: in vitro testing, high dilution, B16-F10, melanoma.

scholarly journals Effects of Vitrification on the Blastocyst Gene Expression Profile in a Porcine Model

2021 ◽  
Vol 22 (3) ◽  
pp. 1222
Author(s):  
Cristina Cuello ◽  
Cristina A. Martinez ◽  
Josep M. Cambra ◽  
Inmaculada Parrilla ◽  
Heriberto Rodriguez-Martinez ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Analysis ◽  
Survival Rates ◽  
Control Group ◽  
P Value ◽  
Transcriptome Profile ◽  
Embryo Viability ◽  
The Impact

This study was designed to investigate the impact of vitrification on the transcriptome profile of blastocysts using a porcine (Sus scrofa) model and a microarray approach. Blastocysts were collected from weaned sows (n = 13). A total of 60 blastocysts were vitrified (treatment group). After warming, vitrified embryos were cultured in vitro for 24 h. Non-vitrified blastocysts (n = 40) were used as controls. After the in vitro culture period, the embryo viability was morphologically assessed. A total of 30 viable embryos per group (three pools of 10 from 4 different donors each) were subjected to gene expression analysis. A fold change cut-off of ±1.5 and a restrictive threshold at p-value < 0.05 were used to distinguish differentially expressed genes (DEGs). The survival rates of vitrified/warmed blastocysts were similar to those of the control (nearly 100%, n.s.). A total of 205 (112 upregulated and 93 downregulated) were identified in the vitrified blastocysts compared to the control group. The vitrification/warming impact was moderate, and it was mainly related to the pathways of cell cycle, cellular senescence, gap junction, and signaling for TFGβ, p53, Fox, and MAPK. In conclusion, vitrification modified the transcriptome of in vivo-derived porcine blastocysts, resulting in minor gene expression changes.

Endothelin-3 Is Produced by Metastatic Melanoma Cells and Promotes Melanoma Cell Survival

2008 ◽  
Vol 12 (2) ◽  
pp. 64-70 ◽  
Author(s):  
Liren Tang ◽  
Mingwan Su ◽  
Yi Zhang ◽  
Wency Ip ◽  
Magdalena Martinka ◽  
...  
Keyword(s):  
Metastatic Melanoma ◽  
Signal Transduction Pathway ◽  
Melanoma Cells ◽  
Transduction Pathway ◽  
Paracrine Factor ◽  
Study Results ◽  
Autocrine Stimulation ◽  
Endothelin 3 ◽  
Adult Skin

Background: Endothelin-3 (ET-3) is an essential paracrine factor for the proliferation, migration, and survival of embryonic melanocytes during fetal development. Its expression is tightly regulated, being completely turned off in adult skin. Objective: In this study, results are presented that demonstrate abnormal expression of ET-3 by metastatic melanoma cells in both tissue biopsies and cell culture. Further, in vitro experiments showed that metastatic melanoma cells have the capacity to respond to ET-3 stimulation by increasing survival. Conclusion: Therefore, an abnormal autocrine stimulation pathway involving ET-3 is present in metastatic melanoma cells. Blocking this signal transduction pathway may prove useful for the treatment of metastatic melanoma.

scholarly journals Targeted photodynamic therapy treatment of in vitro A375 metastatic melanoma cells

Oncotarget ◽  
2019 ◽  
Vol 10 (58) ◽  
pp. 6079-6095 ◽  
Author(s):  
Channay Naidoo ◽  
Cherie Ann Kruger ◽  
Heidi Abrahamse
Keyword(s):  
Photodynamic Therapy ◽  
Metastatic Melanoma ◽  
Melanoma Cells ◽  
Targeted Photodynamic Therapy ◽  
Therapy Treatment

scholarly journals Advances in the Treatment and Prevention of Chemotherapy-Induced Ovarian Toxicity

2020 ◽  
Vol 21 (20) ◽  
pp. 7792
Author(s):  
Hyun-Woong Cho ◽  
Sanghoon Lee ◽  
Kyung-Jin Min ◽  
Jin Hwa Hong ◽  
Jae Yun Song ◽  
...  
Keyword(s):  
Cancer Patients ◽  
Fertility Preservation ◽  
Ovarian Tissue ◽  
Alkylating Agents ◽  
Survival Rates ◽  
Chemotherapeutic Agents ◽  
Reproductive Age ◽  
Experimental Techniques ◽  
Ovarian Tissue Cryopreservation

Due to improvements in chemotherapeutic agents, cancer treatment efficacy and cancer patient survival rates have greatly improved, but unfortunately gonadal damage remains a major complication. Gonadotoxic chemotherapy, including alkylating agents during reproductive age, can lead to iatrogenic premature ovarian insufficiency (POI), and loss of fertility. In recent years, the demand for fertility preservation has increased dramatically among female cancer patients. Currently, embryo and oocyte cryopreservation are the only established options for fertility preservation in women. However, there is growing evidence for other experimental techniques including ovarian tissue cryopreservation, oocyte in vitro maturation, artificial ovaries, stem cell technologies, and ovarian suppression. To prevent fertility loss in women with cancer, individualized fertility preservation options including established and experimental techniques that take into consideration the patient’s age, marital status, chemotherapy regimen, and the possibility of treatment delay should be provided. In addition, effective multidisciplinary oncofertility strategies that involve a highly skilled and experienced oncofertility team consisting of medical oncologists, gynecologists, reproductive biologists, surgical oncologists, patient care coordinators, and research scientists are necessary to provide cancer patients with high-quality care.

scholarly journals Effect of different periodontal ligament simulating materials on the incidence of dentinal cracks during root canal preparation

2018 ◽  
Vol 12 (3) ◽  
pp. 196-200
Author(s):  
Ajita Rathi ◽  
Prateeksha Chowdhry ◽  
Mamta Kaushik ◽  
Pallavi Reddy ◽  
Roshni Roshni ◽  
...  
Keyword(s):  
Root Canal ◽  
Periodontal Ligament ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
P Value ◽  
Root Canal Preparation ◽  
Impression Material ◽  
Group 1

Background. The present study was undertaken to evaluate the incidence of dentinal cracks during root canal preparation with different periodontal ligament simulating materials in vitro. Methods. Seventy freshly extracted human mandibular first premolars were selected and divided into 7 groups in terms of simulating material: group 1: polyether impression material; group 2: polyvinyl acetate adhesive; group 3: polyvinyl siloxane impression material; group 4: cyanoacrylate adhesive; group 5: epoxy resin adhesive; group 6: positive control, without any periodontal ligament simulation; and group 7: negative control, where neither a periodontal ligament simulating material was used nor canal preparation was carried out. Root canal preparation was carried out in all the groups followed by sectioning of roots at 3 mm, 6 mm and 9 mm. The sections were evaluated under a stereomicroscope at ×2.5 for the presence or absence of cracks. Chi-squared test was used to compare the appearance of defective roots between the different experimental groups. Results. The least number of cracks were found in the negative control group, followed by group 1 where polyether impression material was used for periodontal ligament simulation. The difference was significant with a P-value of 0.002 for coronal sections. Conclusion. Under the limitation of the present study, polyether and polyvinyl siloxane (light body) can both be used for simulation of periodontal ligament.

scholarly journals In Vitro and In Vivo Tumor Growth Inhibition by Glutathione Disulfide Liposomes

2017 ◽  
Vol 10 ◽  
pp. 117906441769607 ◽  
Author(s):  
Satya S Sadhu ◽  
Shenggang Wang ◽  
Rakesh Dachineni ◽  
Ranjith Kumar Averineni ◽  
Yang Yang ◽  
...  
Keyword(s):  
Cancer Metastasis ◽  
Cell Function ◽  
Survival Rates ◽  
Significant Inhibition ◽  
Control Group ◽  
Tumor Growth Inhibition ◽  
Tumor Proliferation ◽  
Glutathione Disulfide

Glutathione disulfide (GSSG) is an endogenous peptide and the oxidized form of glutathione. The impacts of GSSG on cell function/dysfunction remain largely unexplored due to a lack of method to specifically increase intracellular GSSG. We recently developed GSSG liposomes that can specifically increase intracellular GSSG. The increase affected 3 of the 4 essential steps (cell detachment, migration, invasion, and adhesion) of cancer metastasis in vitro and, accordingly, produced a significant inhibition of cancer metastasis in vivo. In this investigation, the effect of GSSG liposomes on cancer growth was investigated with B16-F10 and NCI-H226 cells in vitro and with B16-F10 cells in C57BL/6 mice in vivo. Experiments were conducted to elucidate the effect on cell death through promotion of apoptosis and the effect on the cell cycle. The in vivo results with C57BL/6 mice implanted subcutaneously with B16-F10 cells showed that GSSG liposomes retarded tumor proliferation more effectively than that of dacarbazine, a chemotherapeutic drug for the treatment of melanoma. The GSSG liposomes by intravenous injection (GLS IV) and GSSG liposomes by intratumoral injection (GLS IT) showed a tumor proliferation retardation of 85% ± 5.7% and 90% ± 3.9%, respectively, compared with the phosphate-buffered saline (PBS) control group. The median survival rates for mice treated with PBS, blank liposomes, aqueous GSSG, dacarbazine, GLS IV, and GLS IT were 7, 7, 7.5, 7.75, 11.5, and 16.5 days, respectively. The effective antimetastatic and antigrowth activities warrant further investigation of the GSSG liposomes as a potentially effective therapeutic treatment for cancer.

hSRBC promoter CpG island hypermethylation as resistant predictive biomarker of oxaliplatin based chemotherapy in metastasic colorectal cancer patients.

2013 ◽  
Vol 31 (15_suppl) ◽  
pp. e14609-e14609
Author(s):  
Catia Moutinho ◽  
Anna Martinez-Cardus ◽  
Cristina Santos ◽  
Valentín Navarro-Perez ◽  
Eva Martinez-Balibrea ◽  
...  
Keyword(s):  
Cox Regression ◽  
Cpg Island ◽  
Methylation Status ◽  
Stage Iv ◽  
Predictive Biomarker ◽  
Promoter Hypermethylation ◽  
Chemotherapeutic Agents ◽  
P Value ◽  
Potential Biomarker

e14609 Background: Resistance acquisition to chemotherapeutic agents is one of the main problems that come up during cancer treatment. hSRBC is a tumor suppressor gene whose inactivation has been associated with malignant tumor progression. In a previous work, we investigated the influence of hSRBC promoter methylation alterations in oxaliplatin (OXA) resistance acquisition by using a CRC “in vitro” model, detecting an hSRBC promoter hypermethylation in OXA–resistant derived cells when compared with the sensitive counterpart. These results were validated by functional analyses in the same model. Taking this into account, our aim in the present work is to determine the role of hSRBC methylation status as a potential biomarker of OXA resistance, in metastatic CRC patients, treated at first line with fluouropirimidines plus OXA based chemotherapy. Methods: hSRBC promoter hypermethylation was analyzed in DNA extracted from paraffin embebbed tissue of 111 metastatic CRC tumors by using Methylation Specific PCR. Methylation data was correlated to overall response (OR) and progression free survival (PFS) by using F-Fisher test and Kaplan-Meyer Survival curves respectively. A multivariate analysis was carried out by Cox regression. p-values under 0.05 were considered statistic significant. Results: Two independent cohorts of stage IV CRC tumors were included. Twenty-two out of 111 patients received radical surgery for metastasis, that became to be a positive prognostic factor (p-value = 0.04). Gene hypermethylation was detected in a 33% of cases. Although OR was not associated with hSRBC methylation, we observed a significant correlation between hypermethylation of gene and a worse PFS in patients without metastasis surgery (Log Rank; p-value = 0.04). Conclusions: Remarkably, hSRBC promoter hypermethylation is associated with worse PFS in metastatic CRC patients. We suggest hSRBC methylation status as a predictive biomarker of OXA-based treatment outcome in metastatic CRC patients. However, further studies are warranted in order to elucidate the clinical application of these findings.

In vitro sensitivity of human melanoma cells to chemotherapeutic agents and interferons

1994 ◽  
Vol 4 (4) ◽  
pp. 243-249 ◽  
Author(s):  
D Schadendorf ◽  
K Jurgovsky ◽  
M Worm ◽  
B M Czarnetzki
Keyword(s):  
Melanoma Cells ◽  
Chemotherapeutic Agents ◽  
Human Melanoma ◽  
In Vitro Sensitivity ◽  
Human Melanoma Cells

scholarly journals Synthesis of Novel Cinnamides and a Bis Cinnamate Bearing 1,2,3-Triazole Functionalities with Antiproliferative and Antimetastatic Activities on Melanoma Cells

2021 ◽  
Author(s):  
Fabíola Santos ◽  
Juliana do Vale ◽  
Lucas Santos ◽  
Talita Gontijo ◽  
Graziela Lima ◽  
...  
Keyword(s):  
Melanoma Cell ◽  
High Resolution Mass Spectrometry ◽  
Melanoma Cells ◽  
Chemotherapeutic Agents ◽  
Cycle Arrest ◽  
13C Nuclear Magnetic Resonance ◽  
Metastatic Behavior ◽  
Melanoma Treatment ◽  
Murine Melanoma Cell Line

The present investigation describes the synthesis of novel cinnamides and a bis cinnamate bearing 1,2,3-triazole functionalities and investigation of their antiproliferative and antimetastatic effects on melanoma cells. The necessity for the development of new chemotherapeutic agents for melanoma treatment motivated this work. Sixteen derivatives were obtained with yields ranging from 23-81% and fully characterized by spectroscopic (1H and 13C nuclear magnetic resonance, infrared) and spectrometric high resolution mass spectrometry (HRMS) techniques. The derivatives were in vitro evaluated against B16-F10 murine melanoma cell line. The most effective compound (a bis cinnamate) (6b) reduced the melanoma cell viability, generated cell cycle arrest, and influenced the metastatic behavior of melanoma cells by decreasing migration, invasion, and colony formation. Based on these findings, it is believed that compound 6b may represent an interesting scaffold to be explored toward the development of new antimelanoma agents.

scholarly journals Analysis of Surface Roughness and 3D Scanning Topography of Zirconia Implants Before and After Photofunctionalisation by 3D Optical Profilometry- An Invitro Study

2021 ◽  
Author(s):  
Arunjaikumar Ravindran ◽  
Suma Karthigeyan ◽  
Ramesh Bhat ◽  
Madhulika Naidu ◽  
Senthilnathan Natarajan ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Roughness Parameter ◽  
In Vitro Study ◽  
Control Group ◽  
P Value ◽  
Study Group ◽  
Optical Profilometry ◽  
Before And After ◽  
Zirconia Implants

Introduction: Zirconia is considered as an alternative material for the fabrication of implants. Surface roughness of the implant plays a fundamental role in the initial bone formation. The rationale of using 3D optical profilometry in this study was to evaluate the surface roughness of the zirconia implants before and after Ultraviolet (UV) photofunctionalisation. Also, Moreso 3D optical instruments have a better resolution than the mechanical ones like Atomic Force Microscopy (AFM). Aim: To analyse the surface roughness and topography of zirconia implants after photofunctionalisation using optical profilometry. Materials and Methods: This is an in-vitro study conducted over a period of six months from March 2020 to August 2020. Ten commercially machined Zirconia implants, five each in study and control group, were micro analysed at three different regions (abutment, thread and crest) by optical profilometry. Study group was surface treated by UV radiation for 48 hours. Quantitative morphometric analysis was done between two groups and p-value less than 0.05 was considered statistically significant. The statistical test applied in this study was independent t-test. Results: Scanning micrographs of the study group revealed highest density of summits contributing to increased surface area in the study group. Quantitative analysis of surface roughness showed statistically significant higher mean roughness parameter for photofunctionalised implants in abutment, crest and thread region (p<0.05). Conclusion: Photofunctionalisation is a potentially synergistic technique in producing textured zirconia implants.

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