use of methods of the biocrystallomics in personification of the treatment with high diluted drugs

Background: One of most important trends in modern medicine is its transformation to personalized diagnostics and treatment. This tendency fully applies to homeopathy, especially considering that this discipline is based on the principles of individual approach to the patient. At the same time, the methods of individualization of treatment in homeopathy are relatively few. Aims: Develop of methodology and methods of application biocrystalloscopic tests to personalize the homeopathic treatment. The proposed technology became previously proposed for ozone therapy practice as biocrystallomics pre-test. It includes study of the result of co-crystallization of a biological liquid of a patient with the intended dose of the drug. At the same time, the character of co-crystallization of biological liquid with several doses of tested drug or different parameters of the action of the studied factor is comparatively estimated for the purpose of individualization. Most appropriate for a particular patient believe the dose that causes optimal structuring in the dried microscopic slides obtained from a mixture of biological fluid and a solution containing a given dose of the drug. The comparison is made with a control sample of biological fluid, which has not been exposed to any effects. The optimal result is a sample that matches the control sample as much as possible. It is most preferably for biocrystallomics pre-test to use blood serum or plasma as an analyzed biological fluid. Methodology: To study the characteristics of the structuring of the semicroscopic slides we use the previously created system of semi-quantitative parameters. They are calculated on a straight four-point scale and include: crystallizability (semi-quantitative indicator of crystallization activity), structure index (complexity criterion for constructing crystal elements of facias), facia destruction degree (indicator of “correctness” of crystal formation) and clearity of facia marginal zone (parameter indicating the amount of native protein in the sample of biological fluid). These indicators comprehensively characterize all the main features of the process of dehydration structurization of biological substrate. Conclusion: It should be noted that the technique described above is universal and can be applied to any liquid or soluble compounds with potential therapeutic activity, including their ultra-high dilutions. The data obtained by us indicate the possibility of using this technology in homeopathy.

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Influence of porosity on filtration of biological fluid through a two-layer capillary wall

Izvestiya vysshikh uchebnykh zavedenii. Fizika ◽

10.17223/00213411/64/3/40 ◽

2021 ◽

pp. 40-46

Author(s):

N.N. Nazarenko ◽

◽

A.G. Knyazeva ◽

Keyword(s):

Biological Fluid ◽

Fluid Velocity ◽

Capillary Wall ◽

Model Parameters ◽

Steady State Mode ◽

Biological Liquid ◽

Phase Mobility ◽

Different Characteristics ◽

Viscosity Dependence ◽

Filtration Model

On the basis of proposed new filtration model the peculiarities of concentration distribution of component carried by two-component biological liquid and fluid velocity in capillary with two-layer porous walls in steady-state mode are studied. Mathematical model takes into account such important phenomena as concentration expansion and viscosity dependence on concentration. The fluid flow corresponds to the Brinkman model. Dimensionless complexes linking characteristic physical scales of different phenomena are highlighted. Influence of model parameters on biological liquid filtration process for capillary wall layers with different porosity is analyzed. The peculiarities of flow regime and distribution of component concentration for different characteristics of internal porous layer (porosity, phase mobility, size) are revealed.

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The Critical Point Drying Method Applied to Scanning Electron Microscopy of L-929 Cells

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100068382 ◽

1970 ◽

Vol 28 ◽

pp. 278-279

Author(s):

Charles TurnbiLL ◽

Delbert E. Philpott

Keyword(s):

Electron Microscopy ◽

Carbon Dioxide ◽

Surface Tension ◽

Critical Point ◽

Freeze Drying ◽

Attractive Alternative ◽

Crystal Formation ◽

Critical Point Drying ◽

Time Required ◽

Scanning Electron

The advent of the scanning electron microscope (SCEM) has renewed interest in preparing specimens by avoiding the forces of surface tension. The present method of freeze drying by Boyde and Barger (1969) and Small and Marszalek (1969) does prevent surface tension but ice crystal formation and time required for pumping out the specimen to dryness has discouraged us. We believe an attractive alternative to freeze drying is the critical point method originated by Anderson (1951; for electron microscopy. He avoided surface tension effects during drying by first exchanging the specimen water with alcohol, amy L acetate and then with carbon dioxide. He then selected a specific temperature (36.5°C) and pressure (72 Atm.) at which carbon dioxide would pass from the liquid to the gaseous phase without the effect of surface tension This combination of temperature and, pressure is known as the "critical point" of the Liquid.

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Techniques for cryoultramicrotomy of propane jet frozen biological samples

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010014292x ◽

1986 ◽

Vol 44 ◽

pp. 260-261

Author(s):

B. Craig ◽

L. Hawkey ◽

A. LeFurgey

Keyword(s):

Freeze Fracture ◽

Freeze Substitution ◽

Heart Cells ◽

Crystal Formation ◽

X Ray ◽

Transmission Electron ◽

Scanning Transmission ◽

Chick Heart ◽

Embryonic Chick Heart ◽

A New Technique

Ultra-rapid freezing followed by cryoultramicrotomy is essential for the preservation of diffusible elements in situ within cells prior to scanning transmission electron microscopy and quantitative energy dispersive x-ray microanalysis. For cells or tissue fragments in suspension and for monolayer cell cultures, propane jet freezing provides cooling rates greater than 30,000°C/sec with regions up to 40μm in thickness free of significant ice crystal formation. While this method of freezing has frequently been applied prior to freeze fracture or freeze substitution, it has not been widely utilized prior to cryoultramicrotomy and subsequent x-ray microanalytical studies. This report describes methods devised in our laboratory for cryosectioning of propane jet frozen kidney proximal tubule suspensions and cultured embryonic chick heart cells, in particular a new technique for mounting frozen suspension specimens for sectioning. The techniques utilize the same specimen supports and sample holders as those used for freeze fracture and freeze substitution and should be generally applicable to any cell suspension or culture preparation.

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High-pressure freezing and freeze substitution of plant tissue

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100124148 ◽

1992 ◽

Vol 50 (1) ◽

pp. 748-749

Author(s):

William P. Sharp ◽

Robert W. Roberson

Keyword(s):

High Pressure ◽

Cell Structure ◽

Leaf Tissue ◽

Freeze Substitution ◽

Crystal Formation ◽

Ice Crystal ◽

High Pressure Freezing ◽

Cell Components ◽

Cold Metal ◽

Brome Grass

The aim of ultrastructural investigation is to analyze cell architecture and relate a functional role(s) to cell components. It is known that aqueous chemical fixation requires seconds to minutes to penetrate and stabilize cell structure which may result in structural artifacts. The use of ultralow temperatures to fix and prepare specimens, however, leads to a much improved preservation of the cell’s living state. A critical limitation of conventional cryofixation methods (i.e., propane-jet freezing, cold-metal slamming, plunge-freezing) is that only a 10 to 40 μm thick surface layer of cells can be frozen without distorting ice crystal formation. This problem can be allayed by freezing samples under about 2100 bar of hydrostatic pressure which suppresses the formation of ice nuclei and their rate of growth. Thus, 0.6 mm thick samples with a total volume of 1 mm3 can be frozen without ice crystal damage. The purpose of this study is to describe the cellular details and identify potential artifacts in root tissue of barley (Hordeum vulgari L.) and leaf tissue of brome grass (Bromus mollis L.) fixed and prepared by high-pressure freezing (HPF) and freeze substitution (FS) techniques.

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The “KIMWIPE” Effect in Ultra-Thin Cryosections

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100119119 ◽

1985 ◽

Vol 43 ◽

pp. 458-459

Author(s):

I. Taylor ◽

P. Ingram ◽

J.R. Sommer

Keyword(s):

Skeletal Muscle ◽

Electrical Stimulation ◽

Muscle Fibers ◽

Ice Crystals ◽

Crystal Formation ◽

Ice Crystal ◽

Thin Sections ◽

Skeletal Muscle Fibers ◽

Freeze Dried ◽

Ice Crystal Formation

In studying quick-frozen single intact skeletal muscle fibers for structural and microchemical alterations that occur milliseconds, and fractions thereof, after electrical stimulation, we have developed a method to compare, directly, ice crystal formation in freeze-substituted thin sections adjacent to all, and beneath the last, freeze-dried cryosections. We have observed images in the cryosections that to our knowledge have not been published heretofore (Figs.1-4). The main features are that isolated, sometimes large regions of the sections appear hazy and have much less contrast than adjacent regions. Sometimes within the hazy regions there are smaller areas that appear crinkled and have much more contrast. We have also observed that while the hazy areas remain still, the regions of higher contrast visibly contract in the beam, often causing tears in the sections that are clearly not caused by ice crystals (Fig.3, arrows).

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HREM observation of NiO growth on submicron spheres of pure nickel

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100154718 ◽

1989 ◽

Vol 47 ◽

pp. 548-549

Author(s):

C.M. Teng ◽

T.F. Kelly ◽

J.P. Zhang ◽

H.M. Lin ◽

Y.W. Kim

Keyword(s):

Nickel Oxide ◽

Grain Boundaries ◽

Pure Nickel ◽

Submicron Particles ◽

Crystal Formation ◽

Nickel Wire ◽

Liquid Droplets ◽

Oxide Interface ◽

Nickel Chromium ◽

Chromium Alloys

Spherical submicron particles of materials produced by electrohydrodynamic (EHD) atomization have been used to study a variety of materials processes including nucleation of alternative crystallization phases in iron-nickel and nickel-chromium alloys, amorphous solidification in submicron droplets of pure metals, and quasi-crystal formation in nickel-chromium alloys. Some experiments on pure nickel, nickel oxide single crystals, the nickel/nickel(II) oxide interface, and grain boundaries in nickel monoxide have been performed by STEM. For these latter studies, HREM is the most direct approach to obtain particle crystal structures at the atomic level. Grain boundaries in nickel oxide have also been investigated by HREM. In this paper, we present preliminary results of HREM observations of NiO growth on submicron spheres of pure nickel.Small particles of pure nickel were prepared by EHD atomization. For the study of pure nickel, 0.5 mm diameter pure nickel wire (99.9975%) is sprayed directly in the EHD process. The liquid droplets solidify in free-flight through a vacuum chamber operated at about 10-7 torr.

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Adolescents’ self-mutilation - Relationship with dependent behaviour

Swiss Journal of Psychology ◽

10.1024/1421-0185.62.4.241 ◽

2003 ◽

Vol 62 (4) ◽

pp. 241-249 ◽

Cited By ~ 8

Author(s):

M. Bolognini ◽

B. Plancherel ◽

J. Laget ◽

P. Stéphan ◽

O. Halfon

Keyword(s):

Suicide Attempts ◽

Clinical Sample ◽

Control Sample ◽

Clinical Population ◽

Control Group ◽

Clinical Group ◽

Self Mutilation ◽

Self Harm ◽

Partial Overlap ◽

The Relationship

The aim of this study, which was carried out in the French-speacking part of Switzerland, was to examine the relationship between suicide attempts and self-mutilation by adolescents and young adults. The population, aged 14-25 years (N = 308), included a clinical sample of dependent subjects (drug abuse and eating disorders) compared to a control sample. On the basis of the Mini Neuropsychiatric Interview ( Sheehan et al., 1998 ), DSM-IV criteria were used for the inclusion of the clinical population. The results concerning the occurrence of suicide attempts as well as on self-mutilation confirm most of the hypotheses postulated: suicidal attempts and self-mutilation were more common in the clinical group compared to the control group, and there was a correlation between suicide attempts and self-mutilation. However, there was only a partial overlap, attesting that suicide and self-harm might correspond to two different types of behaviour.

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