Does it pay off to link functional gene expression to denitrification rates via modelling?
<p>The abundances of functional genes and transcripts have provided new insights into microbially mediated biogeochemical processes and might improve quantitative predictions of turnover rates.<br>However, the relationship between reaction rates and the gene and transcript abundances may not be a simple correlation.<br>Most mechanistic reaction models cannot predict molecular-biological data, and it is unclear how they can be informed by such data.</p><p>We developed a mechanistic model that considers transcript abundances of denitrification genes, enzyme concentrations, biomass, and solute concentrations as state variables that are interrelated by ordinary differential equations, and thus mechanistically links molecular-biological data to reaction rates.<br>Important features of transcript dynamics can be reproduced with the transcript-based model.</p><p>We calibrated the model using data from a batch experiment with a denitrifying organism at the onset of anoxia.<br>We explored the relationship between transcript abundances and reaction rates by analyzing the model results.<br>The transcript abundances reacted very quickly to substrate concentrations so that we could simplify the model by assuming a quasi steady state of the transcripts.</p><p>We compared our model to a classical Monod-type formulation, which was as good at simulating the concentrations of nitrogen species as the transcript-based model, but it cannot make use of any molecular-biological data.<br>Our results, thus, suggest that enzyme kinetics (substrate limitation, inhibition) control denitrification rates more strongly than the dynamics of gene expression.</p>