scholarly journals ALGORITHMS OF CRESCENT STRUCTURE DETECTION IN HUMAN BIOLOGICAL FLUID FACIES

2017 ◽  
Vol XLII-2/W4 ◽  
pp. 169-172
Author(s):  
V. R. Krasheninnikov ◽  
O. E. Malenova ◽  
A. S. Yashina
Keyword(s):  
Medical Diagnosis ◽  
Early Stage ◽  
Biological Fluid ◽  
Biological Fluids ◽  
Health Examination ◽  
Ischemic Disease ◽  
Probability Of False Alarm ◽  
Structure Detection ◽  
Automated Processing ◽  
Mass Health

One of the effective methods of early medical diagnosis is based on the image analysis of human biological fluids. In the process of fluid crystallization there appear characteristic patterns (markers) in the resulting layer (facies). Each marker is a highly probable sign of some pathology even at an early stage of a disease development. When mass health examination is carried out, it is necessary to analyze a large number of images. That is why, the problem of algorithm and software development for automated processing of images is rather urgent nowadays. This paper presents algorithms to detect a crescent structures in images of blood serum and cervical mucus facies. Such a marker indicates the symptoms of ischemic disease. The algorithm presented detects this marker with high probability when the probability of false alarm is low.

scholarly journals Algorithm for detecting spherulite marker in human blood serum facies

2019 ◽  
pp. 109-113
Author(s):  
O E Malenova ◽  
L I Trubnikova ◽  
A S Yashina ◽  
M L Albutova
Keyword(s):  
Thin Film ◽  
Mammary Gland ◽  
Medical Diagnosis ◽  
Visual Analysis ◽  
Biological Fluids ◽  
Uterine Fibroids ◽  
Detection Methods ◽  
Atypical Form ◽  
Probable Presence ◽  
Characteristic Features

One of the effective methods of early medical diagnosis is the method of wedge dehydration. It is based on the analysis of facies images. Facia is a thin film of dried human biological fluids. The presence of special structures (markers) indicates various pathologies of the organism at their earliest stages. In this article, the algorithm for detecting spherulite marker on microscopic images of human serum facies is presented. The presence of spherulites on facies is the norm. However, the atypical form of spherulite is a marker of precancerous diseases: uterine fibroids, endometrial hyperplastic processes and the mammary gland. Due to the visual analysis of the marker, its characteristic features were identified. Then algorithmic detection methods for these features were developed. The decision on the probable presence of a marker was made if there was a combination of features of this marker. As a result of the application of the developed algorithm, most images of atypical spherulites were identified.

scholarly journals Biodegradable Conducting Polymer Coating to Mitigate Early Stage Degradation of Magnesium in Simulated Biological Fluid: An Electrochemical Mechanistic Study

2019 ◽  
Vol 6 (18) ◽  
pp. 4893-4901
Author(s):  
Sina S. Jamali ◽  
Simon E. Moulton ◽  
Yue Zhao ◽  
Sanjeev Gambhir ◽  
Maria Forsyth ◽  
...  
Keyword(s):  
Conducting Polymer ◽  
Polymer Coating ◽  
Early Stage ◽  
Biological Fluid ◽  
Mechanistic Study

scholarly journals Galectin-3 as a Next-Generation Biomarker for Detecting Early Stage of Various Diseases

Biomolecules ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 389 ◽  
Author(s):  
Akira Hara ◽  
Masayuki Niwa ◽  
Kei Noguchi ◽  
Tomohiro Kanayama ◽  
Ayumi Niwa ◽  
...  
Keyword(s):  
Early Stage ◽  
Biological Activities ◽  
Biological Fluids ◽  
Inflammatory Cells ◽  
Tumor Formation ◽  
Term Outcome ◽  
Long Term Outcome ◽  
Galectin 3 ◽  
Binding Lectin

Galectin-3 is a β-galactoside-binding lectin which is important in numerous biological activities in various organs, including cell proliferation, apoptotic regulation, inflammation, fibrosis, and host defense. Galectin-3 is predominantly located in the cytoplasm and expressed on the cell surface, and then often secreted into biological fluids, like serum and urine. It is also released from injured cells and inflammatory cells under various pathological conditions. Many studies have revealed that galectin-3 plays an important role as a diagnostic or prognostic biomarker for certain types of heart disease, kidney disease, viral infection, autoimmune disease, neurodegenerative disorders, and tumor formation. In particular, it has been recognized that galectin-3 is extremely useful for detecting many of these diseases in their early stages. The purpose of this article is to review and summarize the recent literature focusing on the biomarker characteristics and long-term outcome predictions of galectin-3, in not only patients with various types of diseases, but associated animal models.

The Analysis of Phenylbutazone and Its Active Metabolite, Oxyphenbutazone, in Equine Tissues (Muscle, Kidney, and Liver), Urine, and Serum by LC-MS/MS

2017 ◽  
Vol 100 (4) ◽  
pp. 1110-1122
Author(s):  
Joe O Boison ◽  
Patricia Dowling ◽  
Johanna L Matus ◽  
Jana Kinar ◽  
Ron Johnson
Keyword(s):  
Biological Fluid ◽  
Biological Fluids ◽  
Internal Standards ◽  
Tissue Samples ◽  
Once Daily ◽  
Muscle Tissues ◽  
Residue Depletion ◽  
Kidney Liver ◽  
Kinetics Of ◽  
Urine And Serum

Abstract This study reports the use of two validated LC with tandem MS (MS/MS) methods to study the residue depletion profile of phenylbutazone (PBZ) and its metabolite oxyphenbutazone (OXPBZ) from equine serum, urine, and muscle, kidney, and liver tissues. One LC-MS/MS method, with an LOQ of 1.0 ng/mL for PBZ and 2.0 ng/mL for OXPBZ, was used for the analysis of the two drugs in the biological fluids (equine urine and serum); the other LC-MS/MS method, with an LOQ of 0.5 ng/g for PBZ and OXPBZ, was used for the analysis of the drugs in the equine tissue samples. PBZ was administered intravenously to two horses dosed with 8.8 mg/kg PBZ once daily for 4 days and sacrificed humanely at a slaughter plant 7 days after the last drug administration. Urine, serum, and kidney, liver, and muscle tissues were collected from the two horses and shipped on ice to the laboratory and stored at −20°C until analysis. The concentrations of PBZ and OXPBZ residues in the biological fluid and tissue samples collected at slaughter were measured with the two validated LC-MS/MS methods using deuterated internal standards. The results demonstrate that the validated methods are fit for studying the depletion kinetics of PBZ residues in equine tissues and biological fluids.

scholarly journals A Privacy-Preserving Intelligent Medical Diagnosis System Based on Oblivious Keyword Search

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Zhaowen Lin ◽  
Xinglin Xiao ◽  
Yi Sun ◽  
Yudong Zhang ◽  
Yan Ma
Keyword(s):  
Medical Diagnosis ◽  
Keyword Search ◽  
Personal Information ◽  
Privacy Preserving ◽  
Search Process ◽  
Health Examination ◽  
Diagnosis System ◽  
Paillier Cryptosystem ◽  
Medical Diagnosis System ◽  
Computational Ability

One of the concerns people have is how to get the diagnosis online without privacy being jeopardized. In this paper, we propose a privacy-preserving intelligent medical diagnosis system (IMDS), which can efficiently solve the problem. In IMDS, users submit their health examination parameters to the server in a protected form; this submitting process is based on Paillier cryptosystem and will not reveal any information about their data. And then the server retrieves the most likely disease (or multiple diseases) from the database and returns it to the users. In the above search process, we use the oblivious keyword search (OKS) as a basic framework, which makes the server maintain the computational ability but cannot learn any personal information over the data of users. Besides, this paper also provides a preprocessing method for data stored in the server, to make our protocol more efficient.

scholarly journals Nanoparticle-Aided Detection of Colorectal Cancer-Associated Glycoconjugates of Extracellular Vesicles in Human Serum

2021 ◽  
Vol 22 (19) ◽  
pp. 10329
Author(s):  
Rufus Vinod ◽  
Randa Mahran ◽  
Erica Routila ◽  
Janne Leivo ◽  
Kim Pettersson ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Human Serum ◽  
Cell Lines ◽  
Extracellular Vesicles ◽  
Late Stage ◽  
Early Stage ◽  
Biological Fluids ◽  
Serum Samples ◽  
Benign Condition ◽  
Hek 293

Extracellular vesicles (EVs) are found in all biological fluids, providing potential for the identification of disease biomarkers such as colorectal cancer (CRC). EVs are heavily glycosylated with specific glycoconjugates such as tetraspanins, integrins, and mucins, reflecting the characteristics of the original cell offering valuable targets for detection of CRC. We report here on europium-nanoparticle (EuNP)-based assay to detect and characterize different surface glycoconjugates of EVs without extensive purification steps from five different CRC and the HEK 293 cell lines. The promising EVs candidates from cell culture were clinically evaluated on small panel of serum samples including early-stage (n = 11) and late-stage (n = 11) CRC patients, benign condition (n = 11), and healthy control (n = 10). The majority of CRC cell lines expressed tetraspanin sub-population and glycovariants of integrins and conventional tumor markers. The subpopulation of CD151 having CD63 expression (CD151CD63) was significantly (p = 0.001) elevated in early-stage CRC (8 out of 11) without detecting any benign and late-stage samples, while conventional CEA detected mostly late-stage CRC (p = 0.045) and with only four early-stage cases. The other glycovariant assays such as CEACon-A, CA125WGA, CA 19.9Ma696, and CA 19.9Con-A further provided some complementation to the CD151CD63 assay. These results indicate the potential application of CD151CD63 assay for early detection of CRC patients in human serum.

scholarly journals New insights on microRNAs in diabetic nephropathy: potential biomarkers for diagnosis and therapeutic targets

2017 ◽  
Vol 20 (1) ◽  
pp. 42-50 ◽  
Author(s):  
Elena Sergeevna Kamyshova ◽  
Irina Nikolaevna Bobkova ◽  
Irina Mikhailovna Kutyrina
Keyword(s):  
Diabetic Nephropathy ◽  
Early Detection ◽  
Early Stage ◽  
Biological Fluids ◽  
Renal Tissue ◽  
In Vivo Models ◽  
New Biomarkers

Diabetic nephropathy (DN) is a severe complication of diabetes mellitus associated with the progressive deterioration of renal function. Although microalbuminuria is considered as a gold standard for DN diagnosis, it has limited predictive powers and specificity as a diagnostic tool for the early stage of DN. Therefore, new biomarkers are required for the early detection of DN. Studies using in vitro and in vivo models of DN have revealed an important role of microRNAs (miRNAs), short non-coding RNAs that modulate physiological and pathological processes by inhibiting target gene expression, in DN development. Recent studies have shown that the dysregulation of miRNAs, which is associated with the key features of DN, such as the mesangial expansion and accumulation of extracellular matrix proteins, is related to fibrosis and glomerular dysfunction. Thus, the up- and downregulation of miRNA expression in the renal tissue or biological fluids, including urine, may represent new biomarkers for the diagnosis and monitoring of DN progression. In this review, we highlight the significance of miRNAs as biomarkers for the early detection of DN and emphasise their potential role as a therapeutic target.

scholarly journals Exosomal Long NonCoding Rnas as Cancer Biomarkers and Therapeutic Targets

2020 ◽  
Vol 9 (4) ◽  
pp. 297-304
Author(s):  
O. A. Beylerli ◽  
I. F. Gareev ◽  
V. N. Pavlov ◽  
Zhao Shiguang ◽  
Chen Xin ◽  
...  
Keyword(s):  
Early Stage ◽  
Biological Fluids ◽  
Therapeutic Targets ◽  
Membrane Vesicles ◽  
Extensive Study ◽  
Bioactive Molecules ◽  
Public Healthcare ◽  
Bodily Fluids ◽  
Non Coding Rnas

Extensive study of extracellular vesicles began about ten years ago. Exosomes are extracellular membrane vesicles 30–100 nm in diameter secreted by various types of cells and present in most biological fluids. For a long time they were considered non-functional cellular components. However, it has been proven that they serve as a means of intercellular exchange of information. They can move bioactive molecules such as proteins, lipids, RNA, and DNA. Several studies have shown that their contents, including proteins and non-coding nucleic acids, may be of particular interest as biomarkers of diseases. The most promising of all these molecules are non-coding RNAs (ncRNAs), including microRNAs and long non-coding RNAs (lncRNAs). LncRNAs are a large group of non-coding RNAs (ncRNAs) longer than 200 nucleotides. As regulatory factors lncRNAs play an important role in complex cellular processes, such as apoptosis, growth, differentiation, proliferation, etc. Despite many advances in diagnosis and treatment (surgery, radiation therapy, chemotherapy), cancer remains one of the most important public healthcare problems worldwide. Every day brings a better understanding of the role of exosomes in the development of cancer and metastases. Liquid biopsy has been developed as a method for the detection of cancer at an early stage. This is a series of minimally invasive tests of bodily fluids offering the advantage of real-time tracking of the tumour development. In fact, circulating exosomal lncRNAs have been found to be closely linked to processes of oncogenesis, metastasis and treatment. In this paper we review current studies into the functional role of exosomal lncRNAs in cancer and discuss their potential clinical use as diagnostic biomarkers and therapeutic targets for cancer.

scholarly journals On possibility of using routine biochemical techniques for research of alternative biological human liquids

2020 ◽  
Vol 4 (35) ◽  
pp. 54-57
Author(s):  
S. I. Murskiy
Keyword(s):  
Russian Federation ◽  
Clinical Laboratory ◽  
Biological Fluid ◽  
Biological Fluids ◽  
Reproductive Age ◽  
Laboratory Research ◽  
Iso 15189 ◽  
Biochemical Analyzer ◽  
Automatic Biochemical Analyzer ◽  
The Russian Federation

Biochemical studies of alternative human biological fluids are not common in laboratory practice due to the lack of validation methods. The aim of the study was to validatе on semen plasma routine biochemical research methods using an automatic biochemical analyzer. The studies were carried out on a Cobas Integra 400 plus automatic biochemical analyzer (Roche, Switzerland) using original Roche reagents (Switzerland). Materials for the study were 30 samples of semen plasma obtained from clinically healthy men of reproductive age. Planning and organization of validation activities were carried out in accordance with GOST ISO 15189–2015. The functional characteristics of the methods were determined in accordance with Order 45 of the Ministry of Health of the Russian Federation «On a system of measures to improve the quality of clinical laboratory research in healthcare facilities of the Russian Federation», and protocols of the CLSI Institute of Clinical Laboratory Standards (USA). It was possible to establish acceptable levels of linearity, precision and specificity for 31 of the 33 analytes studied. Also, the necessary dilution for 12 analytes was established empirically. Establishing the analytical reliability of biochemical methods for studying semen plasma opens up new possibilities for studying the metabolic characteristics of this biological fluid, and therefore expands the range of possibilities for its use as an object of laboratory research.

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