Peculiarities of clonal micropropagation of kamchatka bilberry (vaccinium praestans lamb.) at the stages of «introduction into in vitro culture» and «proper micropropagation»

2021 ◽  
pp. 14-19
Author(s):  
Irina Borisovna Kuznetsova ◽  
Anton Igorevich Chudetsky ◽  
Sergey Sergeevich Makarov
Keyword(s):  
Clonal Micropropagation

Research of carbohydrate type influence on Lonicera caerulea l. Morphogenetic potential in vitro

2020 ◽  
Vol 61 ◽  
pp. 54-60
Author(s):  
N. D. Orlova ◽  
D. A. Egorova
Keyword(s):  
Multiplication Factor ◽  
Lonicera Caerulea ◽  
Genetic Characteristics ◽  
Clonal Micropropagation ◽  
Morphogenetic Potential

The clonal micropropagation technique of valuable Lonicera caerulea L. varieties (Indigo Gem, Moskovskaya 23, Diana, Jugana) has been improved. The effect of various types and concentrations of carbohydrates on microshoots regeneration of the genus Lonicera L. representatives was studied. It is shown that the concentration of carbohydrates has the greatest effect on microshoots height and the type of carbohydrate nutrition has the greatest effect on multiplication factor. The type of carbohydrate nutrition (42 %) had the greatest influence on multiplication factor. Peculiarities of varieties, genetic characteristics interaction, carbohydrate nutrition type and concentration had a lesser effect (20 %, 14 % and 11 % respectively). The variety Moskovskaya 23 was characterized by the highest microshoots number (49.6 mm). The highest multiplication factor in the studied varieties, regardless of the type of carbohydrate nutrition, was observed at concentration of 40 g/l (11.57).

scholarly journals FLAVONOIDS OF IRIS SIBIRICA L. GROWN IN VITRO CULTURE

2021 ◽  
pp. 301-308
Author(s):  
Olesya Nikolayevna Mazko ◽  
Lyudmila Ivanovna Tikhomirova ◽  
Lyudmila Vladimirovna Shcherbakova ◽  
Natal'ya Grigor'yevna Bazarnova ◽  
Dmitriy Alekseyevich Karpitsky
Keyword(s):  
In Vitro Culture ◽  
High Performance ◽  
Raw Materials ◽  
Quantitative Composition ◽  
Qualitative Composition ◽  
Nutrient Media ◽  
Qualitative And Quantitative ◽  
Clonal Micropropagation ◽  
Intact Plants

The aim of this study was to evaluate the effect of 6-benzylaminopurine (BAP) separately and in interaction with auxins on the change in the qualitative and quantitative composition of flavonoids in the raw materials of regenerating plants Iris sibirica L. Cambridge grade in comparison with aeroponic and intact raw materials using the method of high-performance liquid chromatography. Raw materials of I. sibirica Cambridge variety obtained in vitro culture had a richer qualitative composition of flavonoids than intact plants. The dependence of the accumulation of flavonoids on the concentration of 6-benzylaminopurine in nutrient media was noted. The presence of 13 compounds was observed in extracts of 70% ethyl alcohol from regenerating plants grown at the lowest concentration of BAP (1.0 µM) within the experiment. In quantitative terms, the flavonoid apigenin was maximally determined on a medium with BAP 1 µM, and kaempferol - on media with BAP 5.0 µM, supplemented with auxins. For a medium with 7.5 µM BAP, the lowest variety of compounds was observed (9) and the lowest kaempferol content. Auxins influenced the synthesis of flavonoids. The amount of flavonoids in all variants of the experiment increased by an average of 13% in the presence of auxins. The stages of the technological process of obtaining raw materials I. sibirica Cambridge variety on the basis of clonal micropropagation and cultivation in aeroponics conditions allowed to obtain raw materials that do not contain heavy and toxic metals, are not infected with pathogens and pests. With 1 m2 of useful area of aeroponics for 1 year, it is possible to collect 5 times more raw materials than with field cultivation. According to the qualitative composition of phenolic compounds, aeroponic raw materials are identical to intact plants.

scholarly journals Plantlet formation from internode bases of carnation (Dianthus caryophyllus L.) in vivo - useful to mutation breeding or not?

1978 ◽  
Vol 26 (1) ◽  
pp. 31-40
Author(s):  
J.B.M. Custers
Keyword(s):  
Dianthus Caryophyllus ◽  
Mutation Breeding ◽  
Main Axis ◽  
Main Shoot ◽  
Axillary Shoots ◽  
Bud Formation ◽  
Plantlet Formation ◽  
Clonal Micropropagation

After decapitation of the main shoot and subsequently the axillary shoots of carnation plants, annular zones extending between axils of opposite leaves produced numerous buds. Low temperatures (10-14 deg C) were essential for this bud formation, which was restricted to young internodes. The season affected the time till bud formation, and determined whether the buds were formed from the main axis or the laterals. Indications were found that these buds were adventitious. The possibility of using these buds in mutation breeding, and the possible risks if they are formed during clonal micropropagation in vitro, are discussed. (Abstract retrieved from CAB Abstracts by CABI’s permission)

scholarly journals Effect of Light on Rhizogenesis of Forest Berry Plants during Clonal Micropropagation

2021 ◽  
pp. 520-528
Author(s):  
Sergey Makarov ◽  
Sergey Rodin ◽  
Irina Kuznetsova ◽  
Anton Chudetsky ◽  
Svetlana Tsaregradskaya
Keyword(s):  
In Vitro Rooting ◽  
Light Emitting ◽  
Clonal Micropropagation ◽  
Fluorescent Lamps ◽  
Planting Material ◽  
Medicinal Value ◽  
American Cranberry ◽  
Food Market ◽  
Spectral Ranges

Introduction. Forest berry plants are popular on the food market and in pharmacy for their high nutritional and medicinal value. Plantations of forest berry plants can proliferate on unused lands, including depleted peatlands. Clonal micropropagation is the most effective method for obtaining large quantities of high quality planting material. Light-emitting diodes are highly effective for clonal micropropagation. The research objective was to study the effect of different spectral ranges on the process of root formation of forest berry plants in vitro. Study objects and methods. The research featured regenerant plants of half-highbush blueberry, arctic bramble, American cranberry, European cranberry, lingonberry, and Kamchatka bilberry of different cultivars. A set of experiments made it possible to study the effect of lighting type on the growth and development of the root system of forest berry plants in vitro using white fluorescent lamps, white spectrum LED lamps, and LED lamps with a combination of white, red, and blue spectra at the in vitro rooting stage of clonal micropropagation. Results and its discussion. The largest number (3.4–14.6 pcs.) and the maximum total length (10.0–156.9 cm) of roots were observed under LED lamps with a combination of white, red, and blue spectra. The effect was by 1.1–2.8 and 2.0–4.5 times higher than in the case of white-spectrum LED lamps, and by 2.3–7.0 and 3.3–14.9 times than in the case of fluorescent lamps. Variety and shape proved to have no significant effect on biometric indicators. Conclusion. LED lamps had a positive effect on the process of rhizogenesis of forest berry plants during clonal micropropagation. They appeared to be more effective than fluorescent lamps. The combination of white, blue, and red spectra increased the biometric parameters of plants at the stage of in vitro rooting.

scholarly journals Polyploidy in tissues of plants in vitro of grape somaclones

2021 ◽  
Vol 34 ◽  
pp. 03002
Author(s):  
Viktor Klimenko ◽  
Ekaterina Lushchay ◽  
Valeryi Zlenko
Keyword(s):  
Leaf Area ◽  
Cytogenetic Analysis ◽  
Colchicine Treatment ◽  
Reverse Correlation ◽  
Grape Variety ◽  
Meristematic Tissue ◽  
Clonal Micropropagation

In vitro experimental plants obtained by clonal micropropagation of 9 grape somaclones of 5 original forms were the material for cytogenetic research. A biological microscope XSP-146TP was used for cytogenetic analysis. 823 cases of deviation from diploidy were observed in total. Significant tissue ploidy was observed in the meristematic tissue of in vitro plants of grape somaclones obtained by colchicine treatment of proembryogenic cells of various varieties. The significant direct correlation was found between the frequency of polyploidy in meristem tissues of in vitro plants and the number of chloroplasts in the stomata of grape somaclones. The reverse correlation was found between the frequency of polyploidy and the number of stomata on the leaf area. Somaclone No. 72, obtained as a result of regeneration from colchicinated proembryogenic cells of the Ruta grape variety and identified as a tetraploid (2n = 4x = 76), is recommended for use in the polyploid creation program.

scholarly journals Clonal Micropropagation of Cranberry (Oxycoccus palustris Pers.)

2021 ◽  
Vol 51 (1) ◽  
pp. 67-76
Author(s):  
Sergey Makarov ◽  
Irina Kuznetsova ◽  
Mikhail Upadyshev ◽  
Sergey Rodin ◽  
Anton Chudetsky
Keyword(s):  
Survival Rate ◽  
Nutrient Medium ◽  
Average Length ◽  
Forest Products ◽  
Hybrid Form ◽  
Clonal Micropropagation ◽  
Planting Material ◽  
Total Growth

Introduction. The last decade saw a considerable increase in the demand for European cranberry planting material (Oxyccocus palustris Pers.) among consumers of non-timber forest products. Cranberry possesses high nutritional and medicinal value. Cultivars and hybrids of European cranberry prove extremely productive for plantation growth using the method of clonal micropropagation with revitalized planting material. Study objects and methods. The research featured European cranberry plants of the Dar Kostromy cultivar and its hybrid form 1-15-635. The study focused on the effect of various medications and growth regulators on the biometric profile of European cranberry and its adaptation to non-sterile conditions at all stages of in vivo clonal micropropagation. Results and discussion. During the introduction stage, the highest viability belonged to the explants treated with AgNO3 (95–96%) and Lizoformin 3000 (5%) as the main sterilizing solutions at a 10-min exposure and a 5% solution of Ecosterilizer (1:1) at a 20-min exposure (90–95%). During the micropropagation proper, the number, average length, and total growth of shoots increased as the concentration of cytokinin 2ip in the WPM 1/4 nutrient medium rose from 1.0 to 5.0 mg/L. At the stage of in vitro rooting, the maximal number, average length, and total growth of roots in regenerated plants for both cultivars were observed when Kornerost 5.0 mg/L was added to the WPM 1/4 nutrient medium. At the stage of adaptation to in vivo conditions, Micogel 0.2 mg/L contributed to the highest survival rate (94–100%). Conclusion. During clonal micropropagation in vitro, the biometric profile of European cranberry (Oxyccocus palustris Pers.) and its survival rate under non-sterile conditions in vivo proved to depend on various growth-regulating substances and their concentrations.

scholarly journals Culture medium for mint micropropagation in vitro

2020 ◽  
Author(s):  
N.A. Yegorova ◽  
◽  
M.S. Zagorskaya ◽  
O.V. Yakimova ◽  
◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Culture Medium ◽  
Medium Composition ◽  
Multiplication Rate ◽  
Ms Medium ◽  
Second Stage ◽  
Clonal Micropropagation ◽  
Propagation Technique

The influence of the culture medium composition on the development of explants at the second stage of clonal micropropagation of mint (Mentha canadensis L. K59(4n)) was studied in order to improve the in vitro propagation technique. It was shown that the maximum multiplication rate (11.5) was provided by MS medium supplemented with BAP (1.0 mg/L), IAA (0.5 mg/L) and 2% sucrose.

scholarly journals Use of growth regulators and experimental LED phytoirradiator in clonal micropropagation of garden strawberry (Fragaria × ananassa, Duchesne ex Weston)

2019 ◽  
Vol 20 (4) ◽  
pp. 324-333 ◽  
Author(s):  
M. G. Markova ◽  
E. N. Somova
Keyword(s):  
Experimental Data ◽  
Growth Regulators ◽  
Growth Regulator ◽  
Fragaria Ananassa ◽  
Clonal Micropropagation ◽  
Fluorescent Lamps ◽  
Combined Use ◽  
The Impact ◽  
Reproduction Factor

The article provides experimental data of 2017-2018 study on the effect of growth regulators and LED phytoirradiator on the proliferation and rooting of promising garden strawberry (Fragaria ananassa) varieties in vitro. Micro-shoots of Korona and Brighton strawberry varieties were taken as the object of the research. Strawberry micro-shoots were cultivated under fluorescent lamps in the control variant. A programmable combined blinking LED phytoirradiator was under study. The combined effect of cytokinin and gibberellic acid by adding them to the Murashige and Skoog nutrient medium, as well as the impact of Siliplant and EcoFus growth regulators on strawberry micropropagation has been studied. It was established that in the cultivation of Korona variety the combined use of Siliplant and EcoFus under illumination with LED phytoirradiator provided an increase in the reproduction factor. The coefficient was 5.0 pcs./explant that was 1.7 times higher than the control (3.0 pcs/explant), the LSD05 1.4 pcs/explant. The maximum reproduction factor of remontant strawberry Brighton variety was obtained in the variant with the use of Siliplant and LED phytoirradiator and amounted to 4.9 pcs./explant (4.2 pcs./explant in the control), the LSD05 was 1.5 pcs./ explant. Regardless of the lighting, the use of RibavExtra in all variants under study increased the rooting rate of the strawberry Korona micro-shoots from 92.8 to 99.1%, the LSD05 6.1%. The use of LED phytoirradiator in comparison with the luminescent one (94.3%) provided a significant increase in the rooting rate of the strawberry Korona micro-shoots to 98.1% regardless of the growth regulators used, the LSD05 3.5%. The combined use of LED phytoirradiator and Ribav-Extra growth regulator in concentrations of 1.0 and 1.5 mg/l resulted in rooting of strawberry Korona micro-shoots up to 100%. Regardless of the growth regulator used, the use of LED phytoirradiator in comparison with the luminescent one (88.9%) provided a significant increase in the rooting rate of the strawberry Brighton micro-shoots to 97.2%, the LSD05 4.6%. The rooting rate of the remontant strawberry Brighton microshoots was 100% in the variant with the use of Ribav-Extra in the concentration of 1.0 mg /l combined with LED phytoirradiator 20 days after transplanting for rooting.

Sign in / Sign up

Export Citation Format

Share Document