scholarly journals Assessment of flavonoids and phenolic compound accumulation in invasive Solidago canadensis L. in Slovakia

10.5219/1378 ◽  
2020 ◽  
Vol 14 ◽  
pp. 587-594
Author(s):  
Olga Shelepova ◽  
Yulia Vinogradova ◽  
Olena Vergun ◽  
Olga Grygorieva ◽  
Ján Brindza
Keyword(s):  
Phenolic Compounds ◽  
Therapeutic Potential ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Flavonoid ◽  
Raw Material ◽  
Herbal Remedies ◽  
Total Phenolic ◽  
Solidago Canadensis ◽  
Dpph Method

Solidago canadensis L. was introduced to Europe as an ornamental plant from North America in 1645 and began to spread during the XIX-XX centuries. Nowadays the species is considered the most aggressive invasive species. On the other hand, S. canadensis is considered to be a medicinal plant. The raw material known as Herba Solidaginis includes herbs of S. canadensis, S. gigantea, and S. virgaurea. These species are known for their diuretic, anti-inflammatory, antimicrobial, antioxidant, antispasmodic properties. The purpose of our study was to analyze the chemical compounds and some biological properties of S. canadensis, growing in Slovakia, to evaluate its therapeutic potential. The total phenolic content (TPC) of the extracts from aerial parts of S. canadensis was determined by the Folin-Ciocalteu method. The evaluation of total flavonoid content (TFC) was performed by using a spectrophotometric method. The flavonoids content was expressed as rutin equivalents (mg REs) per g DW vegetal product. The phytochemical profile of S. canadensis extracts was assessed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The radical scavenging activity of samples was measured using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Reducing power of extracts was determined by the phosphomolybdenum method. Total phenolic contents (TPC) and total flavonoid contents (TFC) of the extracts varied from 204.19 to 293.43 mg GAE.g-1 DW, and 64.99 – 175.25 g QE.g-1 DW, respectively; the best results were obtained for ethanol extract. Some phenolic compounds were identified by HPLC with significant amounts of rutin (211.20 µg.mL-1), quercetin (122.08 µg.mL-1), quercitrin (102.50 µg.mL-1) and chlorogenic acid (147.00 µg.mL-1). The DPPH values in the inflorescences were higher than in the leaves: the antioxidant activity of leaf extracts was in the range from 5.34 to 17.16 mg TE.g-1, for inflorescences, this parameter ranged from 6.09 to 19.87 mg TE.g-1. The high total phenolic compounds and flavonoids can be used as a valuable source of phytochemicals in herbal remedies. Our study of S. canadensis, growing in Slovakia, shows the promising potential that can be evaluated as an effective antioxidant and antimicrobial agent in herbal medicines.

scholarly journals Glycemic Response and Bioactive Properties of Gluten-Free Bread with Yoghurt or Curd-Cheese Addition

Foods ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1410
Author(s):  
Carla Graça ◽  
Joana Mota ◽  
Ana Lima ◽  
Ricardo Boavida Ferreira ◽  
Anabela Raymundo ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Glycemic Index ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Glycemic Response ◽  
Gluten Free ◽  
Antioxidant Power ◽  
Bioactive Properties ◽  
Anti Inflammatory

The influence of flour replacement by yogurt or curd-cheese additions (from 10% to 20%, w/w) on the glycemic response and bioactivity improvements of gluten-free bread was evaluated. Starch digestibility, measured by an in vitro digestion model, was applied to determine the effect on starch fractions. The bread glycemic index was calculated. Bread antioxidant capacity (2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) and ferric-ion-reducing antioxidant power (FRAP) methods) and total phenolic compounds were assessed. Anti-inflammatory properties according to enzymatic matrix metalloproteinase (MMP)-9 inhibitory activity were also studied. Considering the higher level of both dairy products tested (20%, w/w) and comparing with control bread results, a reduction of around 35% in the glycemic response of curd cheese bread was achieved, resulting in intermediate index level (glycemic index (GI) 55–69), with yogurt bread still showing a high glycemic index (GI > 70). In terms of bread bioactivity, curd cheese bread expressed better reducing power effects, whereas yogurt bread showed more effective radical-scavenging capacity. An increase in bread phenolic compounds by yogurt (55.3%) and curd cheese (73.0%) additions (at 20%) were also registered. MMP-9 inhibition activity was higher in the dairy bread than in control bread, suggesting an improvement in terms of anti-inflammatory properties. The supplementation of the gluten-free bread by yogurt or curd cheese was shown to be a promising strategy to reduce the glycemic response and to improve the bioactive properties of the bread, that which can contribute to preventive diets of celiac patients and irritable bowel syndrome individuals.

scholarly journals Anti-oxidant activity of methanolic extract of aquatic flowering plant Nymphaea capensis leaf

2020 ◽  
Author(s):  
Mohammad Alam ◽  
Md. Shahrear Biozid ◽  
Md. Faruk ◽  
Md. Jainul Abeden ◽  
Khandoker Usran Ferdous ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Flavonoid ◽  
Flowering Plant ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Anti Oxidant

Abstract Background: Nymphaea capensis is an aquatic flowering plant which is included in the family of Nymphaeaceae. Literature review on the plants of Nymphaeaceae family exhibited significant medicinal activities. Therefore, the objective of the present study is to evaluate possible anti-oxidant activity of crude methanol extract of N. capensis leaf.Method: In antioxidant study, methanolic plant extract was evaluated for 1,1-diphenyl,2-picrylhydrazyl (DPPH) and reducing power capacity. Moreover, total phenolic and total flavonoid content of plant extracts were determined and expressed in mg of gallic acid equivalent per gram of dry sample (mg GAE/g dry weight).Result: In the DPPH free radical scavenging assay, methanol extract showed concentration dependent inhibition of the free radicals. IC50 of Ascorbic acid was 14.84 µg/ml whereas N. capensis was 130.94 µg/ml. In case of reducing capacity, at conc. 62.5, 125, 250, 500, 1000 ug/ml, the absorbances of Ascorbic acid were 0.65, 1.12, 1.45, 1.78 and 1.89 respectively. In case of N. capensis, the absorbances were 0.46, 0.75, 1.04, 1.27 and 1.50 respectively. The extract displayed a concentration dependent increase in reducing power. In the case of total phenolic content, the methanol extract of N. capensis contained a considerable amount of phenolic contents which was 215±7 of GAE mg/g. In the case of total flavonoid content, methanol extract of N. capensis contained significant amount of flavonoid contents which was 184.75±6.78 of GAE mg/g.Conclusion: These results suggested that the methanol extract of N. capensis possess considerable anti-oxidant activity.

scholarly journals Phenolic Compounds and Antioxidant Activity of Castanopsis phuthoensis and Castanopsis grandicicatricata

2016 ◽  
Vol 55 ◽  
pp. 77-87 ◽  
Author(s):  
Phung Thi Tuyen ◽  
Do Tan Khang ◽  
Luong The Minh ◽  
Truong Ngoc Minh ◽  
Pham Thi Thu Ha ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Phenolic Extracts

In this study, total phenolic, flavonoid contents, antioxidant capacity, and phenolic compositions of Castanopsis phuthoensis and Castanopsis grandicicatricata (Fagaceae family) were investigated. It was found that bark extracts were rich of phenolic contents, whereas leaf extracts were abundant of flavonoids. The total phenolics varied from 11.20 to 35.47 mg gallic acid equivalent g-1 dry weight (DW), and the total flavonoids were from 2.24 to 12.55 mg rutin equivalent g-1 DW. The results of antioxidant activity showed that the DPPH (1,1-diphenyl-2-picryl-hydrazyl) radical scavenging activity of the free phenolic extracts were higher than the bound phenolic extracts. Regarding the reducing power and β-carotene bleaching assays, the free phenolic extracts showed remarkably strong antioxidant capacity that were similar to the levels of the standard BHT (dibutyl hydroxytoluene) did. It could be concluded that free phenolic extracts were more effective in antioxidant activities than bound phenolic extracts. A highly significant correlation between phenolic contents and antioxidant activity in extracts were observed. By HPLC analysis, seven phenolic acids were detected including gallic, p-hydroxybenzoic, vanillic, sinapic, p-coumaric, ellagic, and vanillin. Of which, gallic, ellagic, and sinapic acids were the most abundant compounds in the two species. The results suggest C. phuthoensis and C. grandicicatricata contain rich sources of natural antioxidants and phenolic compounds which are probably considered in pharmaceutical use.

scholarly journals Antioxidant and antimicrobial activities of the essential oil and solvent extracts of Mentha pulegium L.

2015 ◽  
Vol 13 (2) ◽  
pp. 109-119 ◽  
Author(s):  
Ivan Palic ◽  
Jovana Ickovski ◽  
Aleksandra Djordjevic ◽  
Violeta Mitic ◽  
Vesna Stankov-Jovanovic ◽  
...  
Keyword(s):  
Essential Oil ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Flavonoid ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Mentha Pulegium ◽  
Antioxidant Power ◽  
Dry Extract

We report the total phenolic (TPC; expressed as gallic acid equivalents, GAE, per milligram of dry extract weight) and the total flavonoid contents (TFC; expressed as quercetin equivalents, QE, per milligram of dry extract weight) and antimicrobial and antioxidant activities of the essential oil and hexane, diethyl ether, ethyl acetate and methanol extracts of Mentha pulegium L. (Lamiaceae) collected in Serbia. The total phenolic content was in the range of 129.43-388.29 ?g GAE/mg, while TFC ranged from 57.81 to 160.94 QE/mg; the highest TPC and TFC were found in the methanol extract. The antimicrobial activity (against five bacteria and two fungi species) of the essential oil and solvent extracts was assessed using disc-diffusion method. However, the studied samples demonstrated a poor antimicrobial potential. The antioxidant activity was screened using five different tests: 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid radical cation decolorization assay (ABTS), total reducing power (TRP), ferric reducing antioxidant power (FRAP) and cupric reducing antioxidant capacity assay (CUPRAC); the methanol extract showed the strongest antioxidant potential. The results of the different antioxidant assays were correlated mutually and with the total flavonoid and total phenolic contents (regression analysis and agglomerative hierarchical clustering).

In Vitro Antioxidant, Anti-Inflammatory And Anti-Microbial Activity of Carica Papaya Seeds

2020 ◽  
pp. 19-38 ◽  
Author(s):  
Pooja G. Singh ◽  
Madhu S. B ◽  
Shailasreesekhar Shailasreesekhar ◽  
Gopenath TS ◽  
Kanthesh M. Basalingappa ◽  
...  
Keyword(s):  
Carica Papaya ◽  
Therapeutic Potential ◽  
Reducing Power ◽  
In Vitro Study ◽  
Antioxidant Property ◽  
Total Phenolic ◽  
Dpph Method ◽  
Papaya Seeds ◽  
Anti Inflammatory

Papaya seeds are reported to have higher therapeutic potential in comparison to the fruits in which they reside. Thus, the present in-vitro study aimed to evaluate and compare the anti-oxidant, anti-inflammatory and anti-microbial effect of seed extracts on Carica papaya L. (Caricaceae). The bioactive form the seeds were sequentially fractionated with hexane, chloroform, diethyl ether, and methanol in the increasing order of polarity. Total phenolic and flavonoid contents were estimated. These extracts were assessed for an antioxidant property by 1, 1-diphenyl-2-picryl-hydroxyl (DPPH) method and reducing power assay was carried out using the FeCl3 method. Inhibition of 15-lipoxygenase (LOX) by these extracts at 5 -25μg to asses anti-inflammatory capacity was studied.

scholarly journals Effect of traditional sun-drying and oven-drying on carotenoids and phenolic compounds of apricot (Prunus armeniaca L.)

2019 ◽  
Vol 3 (6) ◽  
pp. 186-194
Author(s):  
Ala eddine Derardja ◽  
Malika Barkat
Keyword(s):  
Free Radical ◽  
Phenolic Compounds ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Drying Methods ◽  
Oven Drying ◽  
Sun Drying ◽  
Dpph Method

Background: The indubitable role of phytochemicals such as carotenoids and phenolic compounds in human health has prompted the researchers to study the factors affecting the stability and the availability of these compounds. Aims: This study investigates the effect of two drying processes; oven-drying (OD) and traditional sun-drying (TSD) on carotenoids and phenolic compounds of apricots. Material and Methods: OD was performed at 65°C, and TSD was performed by direct exposure of apricot to sunlight at daytime temperatures around 40°C and relative humidity between 25 and 35%, following an Algerian traditional method of drying. Carotenoids and phenolic compounds were extracted, and then total carotenoids (TC), total phenolic compounds (TPC), total flavonoids (TF) and total tannins (TT) were spectrophotometrically quantified. The free radical scavenging activity (FRSA) of the phenolic extracts was measured by the DPPH method. Results: Carotenoids and phenolic compounds were significantly affected by both drying methods. OD decreased TC and TT by 44% and 12%, respectively, and increased TPC and TF by 4%. TDS affected negatively all the measured components, where TC, TPC, TF, and TT decreased by 67%, 15%, 43%, and 36%, respectively. However, the highest FRSA was reported for the TSD apricots (40%) followed by OD apricots (36%), and fresh apricots (32%). Conclusions: The effect of drying on apricot antioxidants depends on the applied drying method and the studied component. The direct sunlight exposure and the duration of drying condemned TSD to be more harmful on carotenoids and phenolic compounds compared to OD, where carotenoids where more fragile during TSD. In addition, OD improved the content of phenolic compounds by improving their extractability. However, TSD apricots seem to be a better source of free radical scavenging compounds. Keywords: Apricot, traditional sun-drying, oven-drying, carotenoids, phenolic compounds.

scholarly journals A Comparative Analysis of Different Varietal of Fresh and Dried Figs by In Vitro Bioaccessibility of Phenolic Compounds and Antioxidant Activities

2021 ◽  
Vol 25 (1) ◽  
pp. 15-30
Author(s):  
Farida Kehal ◽  
Loucif Chemache ◽  
Makhlouf Chaalal ◽  
Meriem Benbraham ◽  
Esra Capanoglu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Gastrointestinal Digestion ◽  
Sun Drying ◽  
Before And After

Abstract Varietal and sun-drying effects on phenolic compounds and their antioxidant activity were investigated during the in vitro gastrointestinal digestion of fresh and dried figs. The total phenolic compounds (phenolic, flavonoids, and proanthocyanidin) and their antioxidant activity (ferric reducing power; free radical scavenging activity DPPH, and phosphomolybdenum test) were evaluated before and after digestion. The total phenolic compounds and the antioxidant capacities of fresh and dried figs obtained before digestion were significantly (p < 0.05) higher than those obtained after digestion. A significant decrease (p < 0.05) of phenolic compounds and their antioxidant activity was observed during different digestion phases (oral phase > gastric phase > intestinal phase). A positive correlation was found between the total phenolic compounds and the antioxidant activity tested for both fresh and dried figs. Furthermore, the results showed that the digestion has no effect on the total phenolic compounds; however, a negative influence of the pH and the enzymes was observed on these compounds and their antioxidant activity. During in vitro gastrointestinal digestion, the varietal and the sun-drying has no significant effect on the phenolic compounds and their antioxidant activity. Likewise, the fresh or dry variety kept a high content before and after the digestion.

scholarly journals Antioxidant activities and phenolic constituents of Cephalotaxus oliveri Mast. aerial parts

2012 ◽  
Vol 77 (4) ◽  
pp. 437-451 ◽  
Author(s):  
Lingbin Zeng ◽  
L.L.P. Vrijmoed
Keyword(s):  
Phenolic Compounds ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Aerial Parts

The present study was to analyze the antioxidant activity and the responsible chemical constituents in Cephalotaxus oliveri Mast. aerial parts. DPPH (2,2-diphenyl-1-picryl hydrazyl), ABTS [2,2?-azinobis(3-ethylbenzothiazoline- 6-sulfonic acid)], reducing power and total phenolic content assays indicate that the methanol extract of the aerial parts is the most potent radical-scavenger and reducing agent with the highest level of phenolic content among the test extracts. The highly positive linear correlations implicate that the four assays have similar capacity to predict the antioxidant potential of the aerial parts and phenolic compounds present contribute significantly to DPPH and ABTS radical scavenging activity and reducing power of the extracts. The plant also displayed considerable superoxide anion radical scavenging activity. LC-MS/MS and GC-MS analyses resulted in the identification of 22 compounds in the methanol extract, among which are 15 phenolic compounds. The total amount of the phenolic compounds in the methanol extract determined by HPLC method was more than 5622.39 ?g/g dry weight. The considerable antioxidant potential and a high content of phenolic antioxidants suggest that C. oliveri aerial parts are a potential source of natural antioxidants.

scholarly journals Anti-oxidant activity of methanolic extract of aquatic flowering plant Nymphaea capensis leaf

2020 ◽  
Author(s):  
Mohammad Alam ◽  
Md. Shahrear Biozid ◽  
Md. Faruk ◽  
Md. Jainul Abeden ◽  
Khandoker Usran Ferdous ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Flavonoid ◽  
Flowering Plant ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Anti Oxidant

Abstract Background: Nymphaea capensis is an aquatic flowering plant which is included in the family of Nymphaeaceae. Literature review on the plants of Nymphaeaceae family exhibited significant medicinal activities. Therefore, the objective of the present study is to evaluate possible anti-oxidant activity of crude methanol extract of N. capensis leaf.Method: In antioxidant study, methanolic plant extract was evaluated for 1,1-diphenyl,2-picrylhydrazyl (DPPH) and reducing power capacity. Moreover, total phenolic and total flavonoid content of plant extracts were determined and expressed in mg of gallic acid equivalent per gram of dry sample (mg GAE/g dry weight).Result: In the DPPH free radical scavenging assay, methanol extract showed concentration dependent inhibition of the free radicals. IC50 of Ascorbic acid was 14.84 µg/ml whereas N. capensis was 130.94 µg/ml. In case of reducing capacity, at conc. 62.5, 125, 250, 500, 1000 ug/ml, the absorbances of Ascorbic acid were 0.65, 1.12, 1.45, 1.78 and 1.89 respectively. In case of N. capensis, the absorbances were 0.46, 0.75, 1.04, 1.27 and 1.50 respectively. The extract displayed a concentration dependent increase in reducing power. In the case of total phenolic content, the methanol extract of N. capensis contained a considerable amount of phenolic contents which was 215±7 of GAE/g. In the case of total flavonoid content, methanol extract of N. capensis contained significant amount of flavonoid contents which was 184.75±6.78 of GAE/g.

scholarly journals Lonicera iberica M. Bieb.: Investigation Antioxidant Activity and Bioactive Chemicals

2021 ◽  
Vol 9 (6) ◽  
pp. 1124-1128
Author(s):  
Fatma Ergün
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Phenolic Substance ◽  
Amount Of Substance ◽  
Phenolic And Flavonoid

In this study, it was investigated the total amounts of phenolic and flavonoid substances and antioxidant activities in different solvent extracts of Lonicera iberica M. Bieb. wild fruit. Total phenolic contents of the extracts were determined as equivalent to gallic acid using Folin-Ciocalteu reagent, and total flavonoid contents as equivalent to quercetin by aluminium nitrate method. In addition, the antioxidant properties of the extracts were determined using free radical scavenging (DPPH) and reducing power (FRAP) methods. The amount of total phenolic substance of L. iberica fruits in hexane and methanol extracts was calculated as 30.96 ± 0.67 mg of GAE / g and 23.70 ± 1.56 mg of GAE / g, respectively. In addition, the amount of total flavonoid substance was calculated as 46.50 ± 8.54 mg of QE / g and 42.09 ± 2.58 mg of QE / g, respectively. It was determined that DPPH radical scavenging activity correlated with total phenolic and flavonoid amount of substance, and L. iberica had a strong antioxidant effect. It is predicted that our study will shed light on new researches, since it is the first study done with L. Iberica fruits in this field.

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